• Title/Summary/Keyword: sugar source

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Investigation on sink/source related traits and their relation of watermelon germplasm to promote use

  • Hwang, Hyun-Chul;Yi, Jung-Yoon;Rhee, Ju-Hee;Hur, On-Sook;Ro, Na-Young;Sung, Jung-Sook;Lee, Ho-Sun;Lee, Jae-Eun;Lee, Sok-Young
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.10a
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    • pp.75-75
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    • 2018
  • Watermelons, Citrullus species(Cucurbitaceae), are native to Africa and have been cultivated since ancient times. T he fruit flesh of wild watermelon is watery, but typically hard-textured, pale-colored and bland or bitter. The familiar sweet dessert watermelons, C. lanatus, featuring non-bitter, tender, well colored flesh, have a narrow genetic base, suggesting that they are originated from a series of selection events in a single ancestral population. In this study, considered as sweet dissert watermelon, genetic resources, C. lanatus, comprising of traditional cultivars and local accessions were collected from 18 different countries in four continents. A total of 60 accessions were characterized morphologically according to RDA genebank descriptors combined with Japan and China, list for 11 qualitative characteristics, leaf length, leaf width, petiole length, petiole diameter-source, stalk end length, stalk diameter, fruit length, fruit diameter, rind thickness, flesh sugar content($^{\circ}brix$), fruit weight-sink, and 6 sink related characters, leaf margin incision-source, fruit shape, fruit skin ground color, fruit skin stain color, fruit skin stain pattern and flesh color-sink, were also investigated. Even though the relatedness between some morphological traits and fruit weight or fruit sweetness showed no significance, the accessions investigated have a great deal of variation for most of the morphological traits. Additionally, the accessions which showed good performance in flesh color and fruit shape (IT271048) and high sugar content of flesh (IT274119, IT290118) above 14brix, were investigated in this experiment. The accessions, which have the information on specific traits including the selected accessions could be introduced, distributed and investigated for further use.

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Source-sink Relationships of Soybean as Influenced by Drought Stress during the Pod and Seed-developing Stage

  • Shin Seong-Hyu;Park Keum-Yong;Shin Sang-Ouk;Lim Sea-Gyu;Ha Tae-Joung;Kim Do-Soon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.4
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    • pp.310-317
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    • 2006
  • This study was conducted to investigate the influence of drought stress during the pod developing and seed filling stage on source-sink relationships of soybean (Glycine max). Drought treatments were imposed by withholding water at the full-pod stage, 19 days after flowering, and then limited watering was relieved at 15 days after the initiation of drought treatment. Soybean seed yield was reduced by 39% mainly due to decreased pod number under drought stress, but the 100-seed weight was relatively less reduced. In spite of the 15-day drought during the full-pod stage, soybean produced good seeds showing similar l00-seed weight, protein, starch and soluble sugar content to those from the well-watered. Although drought during the full-pod stage caused source limitations; i.e. accelerated leaf senescence and reduced leaf soluble sugars, it did not cause limitations of other source characteristics such as SGR and leaf starch level. This is because the reduction in size of sinks, such as pod and seed abortions compensated for source limitations, resulting in balanced source-sink as expressed by LAR and the ratio of leaf area to seed dry weight. Drought stress during the pod developing and seed filling stage did not disrupt the source-sink balance

Lactic Acid Fermentation with Rice Koji as a Carbon Source (탄소원으로서 입국을 이용한 유산균 발효)

  • Park, Suk-Gyun;Ohk, Seung-Ho;Kim, Jin-Man
    • KSBB Journal
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    • v.30 no.1
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    • pp.33-37
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    • 2015
  • Recently, several health benefits of rice wine, makgeolli, were known due to the interest on the traditional Korean liquor and the researches on the rice wine are increasing. Organic acids produced during the process of rice wine fermentation play important roles in the taste and flavor. In this study, we have examined the optimal conditions for lactic acid production in rice koji as a carbon source. Skim milk was also used as a supplementary ingredient for the optimization of lactic acid fermentation. Bacterial growth of Lactobacillus sakei was monitored under this condition. The pH, acidity of the culture and the ethanol tolerance of this bacterium were also tested. Through these experiments, we were able to optimize the growth condition of lactic acid bacteria by the addition of skim milk. This was also able to affect the change of pH, acidity, sugar concentration and alcohol tolerance, which might contribute to the improvement of the quality of rice wine. The optimal condition for the growth was 2 days with 10% (w/v) of skim milk concentration. With these results, it was confirmed that rice koji was an effective carbon source for the growth of lactic acid bacteria.

Changes in Free Sugar, Coixol Contents and Antioxidant Activities of Adlay Sprout (Coix lacryma-jobi L. var. ma-yuen Stapf.) according to Different Growth Stage (생육시기 별 새싹율무의 유리당, 기능성 성분 및 항산화활성 변화)

  • Lee, Hee Jung;Lee, Jeong Hoon;Jung, Jin Tae;Lee, Yun Ji;Oh, Myeong Won;Chang, Jae Ki;Jeong, Heon Sang;Park, Chun Geon
    • Korean Journal of Medicinal Crop Science
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    • v.27 no.5
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    • pp.339-347
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    • 2019
  • Background: Adlay (Coix lacryma-jobi L. var. ma-yuen Stapf.) is an important medicinal and cereal crop that contains high levels of protein, fatty acids and crude fiber, as well as calcium, phosphorus, zinc, minerals, and essential amino acids. Despite its economic significance, little is known about biological activity in adlay sprouts. This study investigated the total free sugar, polyphenol and flavonoid content, and antioxidative activity in adlay sprouts at different growth stages. Methods and Results: The total free sugar and coixol content of germinated adlay sprouts were analyzed by high performance liquid chromatography. The total phenolic content was measured by the Folin and Denis, and flavonoid content by the Davis method. Antioxidative activity was tested by the 2,2-diphenyl-1-picryl hydrazyl method. All measured component, including total phenolic and coixol content ($2.71{\pm}0.02mg/g$ and $59.70{\pm}0.01mg/g$), and antioxidant capacity ($IC_{50}$; $453.93{\mu}g/m{\ell}$) were highest in 3 day old adlay sprouts. In contrast, total free sugar and flavonoid content (3.02% and $0.60{\pm}0.02mg/g$) were highest in sprouts at 5 days after sowing. In addition, there was a strong positive correlation between antioxidative activity and total polyphenol content (r = 0.902, p < 0.001). Conclusions: Adlay sprouts between 3 days and 5 days following germination contain high levels of functional components and free sugar. This study therefore suggests that adlay sprouts can be developed as a valuable health-promoting food and a good source of natural antioxidants.

Purification and Characterization of Bioactivity Compound Acemannan from Aloe vera (알로에 베라로부터 생리 활성 물질인 아세만난 분리 정제와 특성)

  • Ryu, Il-Whan;Sim, Chang-Sup;Lee, So-Young
    • Korean Journal of Pharmacognosy
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    • v.28 no.2
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    • pp.65-71
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    • 1997
  • This study was carried out to purify and to characterize various bioactive material acemannan from Aloe vera. Purified acemannan was mannose (67%) and acetyl group (23%), and the rest of glucose was galactose that consists of long chain polydispered beta-(1, 4) linked mannan polymers. The sugar and acetyl group in the molecule were linked by molar ratio of 3 : 1. This polysaccharide from Aloe vera may provide functional flood and potential drug source with antiviral and immunomodulating properties.

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Ethanol Fermentation by Pichia Stipitis in a Mixture of Pentoses and Hexoses (오탄당과 육탄당의 혼합용액에서 Pichia stipitis에 의한 에탄올 발효)

  • 정봉환;유연우서진호
    • KSBB Journal
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    • v.9 no.4
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    • pp.395-399
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    • 1994
  • P. stipitis CBS5776 was cultivated to examine the characteristics of ethanol fermentation for hexoses (mannose, g1ucose, and galactose) and pentoses(xylose and arabinose). Glucose was the best carbon source among the sugars used in terms of ethanol yield. Glucose was used to produce ethanol with an yield coefficient 0.376g ethanol/g glucose, whereas mannose was converted to produce ethanol with an yield coefficient 0.326g ethanol/g mannose. P. stipitis CBS5776 was also grown in a mixture of sugars to study the pattern of carbon utilization. The yeast utilized glucose and mannose firsts and then galastose and xylose as carbon sources. Arabinose was partially used for biomass when it was present as a sole carbon source, but it was not metabolized at all in a mixture of carbon sources. P. stipitis produced $12.2g/\ell$ ethanol with a yield coefficient 0.332 g ethano1/g sugar in a mixture of sugars.

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Enzymatic saccarification of cellulosic wastes by pectinase

  • Lee, Ji-Eun;Kim, Sam-Gon;Kim, Seong-Jun
    • 한국생물공학회:학술대회논문집
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    • 2003.10a
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    • pp.512-516
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    • 2003
  • The study was aimed to saccharify callulosic waste by pectinase produced from strain KL34 isolated from soil. The enzyme activity in the culture using 1%(w/v) fruit waste as carbon source reached to 3.8 U/ml. In the enzymatic hydrolysis of cellulosic waste, we obtained 9.5g/L reducing sugar in the condition of supernatant containing 5 U/ml enzyme and 10%(w/v) apple rind as substrate. Additionally, in enzymatic hydrolysis of food waste using pectinase from KL34, reducing sugar of 12.7g/L was obtained, indicating enhancement of 1.6 fold compared with that of only cellulase employment.

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Studies on the activities of ALPase, ACPase, ATPase and accumulation of volutin granules upon growth phase in saccharomyces uvarum (Saccharomyces uvarum의 배양시기에 따른 ALPase, ACPase, ATPase 활성도와 volutin과립 축적량)

  • 이기성;최영길
    • Korean Journal of Microbiology
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    • v.23 no.2
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    • pp.90-100
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    • 1985
  • The present study was designed to investigate cellular regulation of phosphate metabolism between catabolically repressed and derepressed states in yeast (Saccharomyces uvarum). The activities of various phospatases and the contents of phosphate compounds were detected according to the culture phase and various phosphate concentrations. As the results, Saccharomyces uvarum derepressed many phosphate metabolizing enzymes such as alkaline phosphatase, acid phosphatase and ATPase more than ten fold simultaneously during catabolic repression (phospgate and sugar starvation). At the same state, the amounts of orthophosphate, nucleotidic labile phosphate and acid soluble polypgosphate were increased, compared to basal levels of normally cultivated cells. $Mg^{++}-stimulated$ type among all phospatases was appeared to have most of the enzyme activity. It could be postulated that $K^+ -stimulated$ alkaline phosphatase was directly or indirectly correlated with the synthesis of acid insoluble polyphosphate $Mg^{++}-stimulated$ phosphatase with the degradation of polyphosphates. In case of cultivation in the medium supplemented with sugar and phosphate (catabolic derepression), phospgatase activities except for alkaline phosphatase were decreased rapidly through the progressive batch culture, After 12 hrs culture, at early exponential phase, the cellular accumulation of acid insoluble polyphosphate increased about 5 fold, compared to those of the starved cells. Under catabolic repression, it could be postulated that intracellular phosphate metabolism was regulated by derepressions of phosphatases. The function of polyphosphate system was shown to compensate the ATP/ADP system as phosphate donor and energy source especially during catabolic repression.

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점토로부터 철불순물의 생물학적 제거에 미치는 탄소원의 영향

  • Lee, Eun-Young;Cho, Kyung-Suk;Ryu, Hee-Wook;Bae, Moo
    • Microbiology and Biotechnology Letters
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    • v.25 no.6
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    • pp.552-559
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    • 1997
  • Fe (III) impurities in clay could be microbially removed by inhabitant dissimilatory Fe (III) reducing microorganisms. Insoluble Fe (III) in clay particles was leached out as soluble reductive form, Fe (II). The microorganisms removed from 10 to 45% of the initial Fe (III) when each sugar was supplemented to be in ranges of 1 - 5 % (w/w; sugar/clay). The microorganisms reduced 2.1 - 12.8 mol of Fe (III) per 100 mol of carbon in sugars metabolized when sugars such as glucose, maltose, and sucrose were used as sole carbon source. Bacillus sp. IRB-W and Pseudomonas sp. IRB-Y were isolated from the enrichment culture of the clay. The isolates were considered to participate in metabolizing organic compounds to fermentative intermediates with relatively little Fe (III) reduction at initial Fe (III) reduction process. By the microbial treatment, the whiteness of the clay was increased form 63.20 to 79.64, whereas the redness was obviously decreased form 13.47 to 3.55. This treatment did not cause any unfavorable modifications in mineralogical compositions of the clay.

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Effect of Additives on Physical Properties of Dover Sole Skin Gelatin Prepared by Ethanol Fractional Precipitation (에탄올처리 찰가자미류껍질 젤라틴의 물리적 특성에 대한 첨가물의 영향)

  • Cho, Soon-Yeong;Ha, Jin-Hwan;Lee, Eung-Ho;Kim, Jin-Soo
    • Applied Biological Chemistry
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    • v.38 no.4
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    • pp.330-333
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    • 1995
  • Effects of additives on physical properties of dover sole skin gelatin prepared by fractional precipitation with ethanol were investigated to obtain basic data for utilizing as food protein source. Physical properties such as gel strength, melting point, gelling point and viscosity of both ethanol- and non-treated gelatins were improved as ferric ion, sugar and ethanol were added to gelatin sol, but were deteriorated as sodium hydroxide and acids were added. Insignificant difference in effects of physical properties on additives such as ferric ion, sodium chloride, sugar, acids and ethanol between ethanol- and non-treated gelatins were not observed.

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