Source-sink Relationships of Soybean as Influenced by Drought Stress during the Pod and Seed-developing Stage

  • Shin Seong-Hyu (Yeongnam Agricultural Research Institute, NICS, RDA) ;
  • Park Keum-Yong (Yeongnam Agricultural Research Institute, NICS, RDA) ;
  • Shin Sang-Ouk (Yeongnam Agricultural Research Institute, NICS, RDA) ;
  • Lim Sea-Gyu (Yeongnam Agricultural Research Institute, NICS, RDA) ;
  • Ha Tae-Joung (Yeongnam Agricultural Research Institute, NICS, RDA) ;
  • Kim Do-Soon (R&D Park, LG Life Sciences, Ltd.)
  • Published : 2006.09.01

Abstract

This study was conducted to investigate the influence of drought stress during the pod developing and seed filling stage on source-sink relationships of soybean (Glycine max). Drought treatments were imposed by withholding water at the full-pod stage, 19 days after flowering, and then limited watering was relieved at 15 days after the initiation of drought treatment. Soybean seed yield was reduced by 39% mainly due to decreased pod number under drought stress, but the 100-seed weight was relatively less reduced. In spite of the 15-day drought during the full-pod stage, soybean produced good seeds showing similar l00-seed weight, protein, starch and soluble sugar content to those from the well-watered. Although drought during the full-pod stage caused source limitations; i.e. accelerated leaf senescence and reduced leaf soluble sugars, it did not cause limitations of other source characteristics such as SGR and leaf starch level. This is because the reduction in size of sinks, such as pod and seed abortions compensated for source limitations, resulting in balanced source-sink as expressed by LAR and the ratio of leaf area to seed dry weight. Drought stress during the pod developing and seed filling stage did not disrupt the source-sink balance

Keywords

References

  1. Ackerson, R. C., V. D. Havelka, and M. G. Boyle 1984. $CO_2-enrichment$ effects on soybean physiology. II. Effects of stage-specific $CO_2$ exposure. Crop Sci. 24 : 1150-1154 https://doi.org/10.2135/cropsci1984.0011183X002400060034x
  2. Board J. E. and Q. Tan. 1995. Assimilatory capacity effects on soybean yield components and pod number. Crop Sci. 35 : 846-851 https://doi.org/10.2135/cropsci1995.0011183X003500030035x
  3. Board, J. E., A. T. Wier, and D. J. Boethel. 1994. Soybean yield reductions caused by defoliation during mid to late seed filling. Agron. J. 86: 1074-1079 https://doi.org/10.2134/agronj1994.00021962008600060027x
  4. Brevedan, R. E. and D. B. Egli. 2003. Short periods of water stress during seed filling, leaf senescence, and yield of soybean. Crop Sci. 43 : 2083-2088 https://doi.org/10.2135/cropsci2003.2083
  5. Chinnusamy, V. and R. Khanna-Chopra. 2003. Effect of heat stress on grain starch content in diploid, tetraploid and hexaploid wheat species. J. Agro. & Crop Sci. 189: 242-249 https://doi.org/10.1046/j.1439-037X.2003.00036.x
  6. Crafts-Brandner, F. E., S. J. Below, J. E. Harper, and R. H. Hageman. 1984. Effects of pod removal on metabolism and senescence of nodulating and nonnodulating soybean isolines. I. Metabolic constituents. Plant Physiol. 75 : 311-317 https://doi.org/10.1104/pp.75.2.311
  7. De Souza, P. I., D. B. Egli, and W. P. Bruening. 1997. Water stress during seed filling and leaf senescence in soybean. Agron. J. 89 : 807-812 https://doi.org/10.2134/agronj1997.00021962008900050015x
  8. Desclaux, D., T. T. Huynh, and P. Roumet. 2000. Identification of soybean plant characteristics that indicate the timing of drought stress. Crop Sci. 40 : 716-722 https://doi.org/10.2135/cropsci2000.403716x
  9. Dybing, C. D., H. Ghisasi, and C. Paech. 1986. Biochemical characterization of soybean ovary growth from anthesis to abscission of aborting ovaries. Plant Physiol. 81 : 1069-1074 https://doi.org/10.1104/pp.81.4.1069
  10. Eagles, C. F. 1971. Changes in net assimilation rate and leaf-area ratio with time in Dactylis glomerata L. Annal. Botany. 35 : 63-74 https://doi.org/10.1093/oxfordjournals.aob.a084468
  11. Egli, D. B. 1999. Variation in leaf starch and sink limitations during seed filling in soybean. Crop Sci. 43 : 2083-208 https://doi.org/10.2135/cropsci2003.2083
  12. Egli, D. B. and W. P. Bruening. 2001. Source-sink relationships, seed sucrose levels and seed growth rates in soybean. Annal. Botany. 88 : 235-242 https://doi.org/10.1006/anbo.2001.1449
  13. Egli, D. B. and Y. Zhenwen. 1991. Crop growth rate and seed number per unit area in soybean. Crop Sci. 31 : 439-442 https://doi.org/10.2135/cropsci1991.0011183X003100020043x
  14. Egli, D. B., R. D. Guffy, L. W. Meckel, and J. E. Leggett. 1985. The effect of source-sink alterations on soybean seed growth. Annal. Botany. 55 : 395-402 https://doi.org/10.1093/oxfordjournals.aob.a086918
  15. Egli, D. B., J. E. Leggett, and A. Cheniae. 1980. Carbohydrate levels in soybean leaves during reproductive growth. Crop Sci. 20 : 468-473 https://doi.org/10.2135/cropsci1980.0011183X002000040012x
  16. Fader, G. M. and H. R. Koller. 1985. Seed growth rate and carbohydrate pool sizes of the soybean fruit. Plant Physiol. 79 : 663-666 https://doi.org/10.1104/pp.79.3.663
  17. FAO/WHO. 2003. FAO food nutrition paper 77. Rome. ISSN 02544725
  18. Fehr, W. R. and C. E. Caviness. 1977. Stages of soybean development. Iowa State University Special Report 80. Iowa State University, Ames, USA
  19. Heatherly, L. G. and R. W. Elmore. 2004. Managing inputs for peak production. pp. 514-523. In: Boerma H. R. and J. E. Specht (Eds), Soybeans: Improvement, production, and uses (3rd ed).. American Soc. of Agronomy, Crop Sci. Soc. of America, and Soil Sci. Soc. of America, Madison, Wisconsin, USA
  20. Heberer, J. A., F. E. Below, and R. H. Hageman. 1985. Drying method effect on leaf chemical constituents of four crop species. Crop Sci. 35: 1063-1069
  21. Huber, S. C., H. Rogers, and F. L. Mowry. 1984. Effects of water stress on photosynthesis and carbon partitioning in soybean plants grown in the field at different $CO_2$ levels. Plant Physiol. 76 : 244-249 https://doi.org/10.1104/pp.76.1.244
  22. Lichtenthaler, H. K. and A. R. Wellburn. 1983. Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem. Soci. Trans. 11 : 591-592 https://doi.org/10.1042/bst0110591
  23. Liu, F. 2004. Physiological regulation of pod set in soybean (Glycine max L. Merr) during drought at early reproductive stages. Ph.D. Thesis. Department of Agricultural Sciences, The Royal Veterinary and Agricultural University, Copenhagen, Denmark
  24. McWilliams, D. A., D. R. Berglund, and G. J. Endres. 1999. Soybean growth and management quick guide. North Dakota State University, Fargo, USA
  25. Miceli, F., S. J. Crafts-Brandner, and D. B. Egli. 1995. Physical restriction of pod growth alters development of soybean plants. Crop Sci. 35 : 1080-1085 https://doi.org/10.2135/cropsci1995.0011183X003500040027x
  26. Morrison, M. J., H. D. Voldeng, and E. R. Cober. 1999. Physiological changes from 58 years of genetic improvement of short-season soybean cultivars in Canada. Agron. J. 91 : 685-689 https://doi.org/10.2134/agronj1999.914685x
  27. Munier-Jolain, N. G., N. M. Munier-Jolain, R. Roche, B. Ney, and C. Duthion. 1998. Seed growth rate in grain legumes I: Effect of photo-assimilate availability on seed growth rate. J. Exp. Botany. 49 : 1963-1969 https://doi.org/10.1093/jexbot/49.329.1963
  28. Nelson, D. W. and L. E. Sommers. 1973. Determination of total nitrogen in plant material. Agron. J. 65 : 109-112 https://doi.org/10.2134/agronj1973.00021962006500010033x
  29. Saitoh, K., T. Mahmood, and T. Kuroda. 1999. Effect of moisture stress at different growth stages on flowering and pod set in determinate and indeterminate soybean cultivars. Japanese J. Crop Sci. 68 : 537-544 https://doi.org/10.1626/jcs.68.537
  30. Serraj, R., T. R. Sinclair, and L. H. Allen. 1998. Soybean nodulation and $N_2$ fixation response to drought under carbon dioxide enrichment. Plant Cell Environ. 21 : 491-500 https://doi.org/10.1046/j.1365-3040.1998.00298.x
  31. Setter, T. L., B. A. Flannigan, and J. Melkonian. 2001. Loss of kernel set due to water deficit and shade in maize: carbohydrate supplies, abscisic acid, and cytokinins. Crop Sci. 41 : 1530-1540 https://doi.org/10.2135/cropsci2001.4151530x
  32. Sionit, N. and P. J. Kramer. 1977. Effect of water stress during different stages of growth of soybeans. Agron. J. 69 : 274-278 https://doi.org/10.2134/agronj1977.00021962006900020018x
  33. Streeter, J. G. 2003. Effects of drought on nitrogen fixation in soybean root nodules. Plant Cell Environ. 26 : 1199-1204 https://doi.org/10.1046/j.1365-3040.2003.01041.x
  34. Upmeyer, D. J. and H. R. Koller. 1973. Diurnal trends in net photosynthesis rate and carbohydrate levels of soybean leaves. Plant Physiol. 51 : 871-874 https://doi.org/10.1104/pp.51.5.871
  35. Watanabe, S., K. Kojima, Y. Ide, and S. Sasaki. 2000. Effects of saline and osmotic stress on praline and sugar accumulation in Populus euphratica in vitro. Plant Cell Tissue Organ Cult. 63 : 199-206 https://doi.org/10.1023/A:1010619503680
  36. Westgate, M. E., J. R. Schussler, D. C. Reicosky, and M. L. Rate. 1989. Effect of water deficits on seed development in soybean: II. Conservation of seed growth rate. Plant Physiol. 91 : 980-985 https://doi.org/10.1104/pp.91.3.980
  37. Zinselmeier, C., B. R. Jeong, and J. S. Boyer. 1999. Starch and the control of kernel number in maize at low water potentials. Plant Physiol. 121 : 25-35 https://doi.org/10.1104/pp.121.1.25