• 제목/요약/키워드: sucrose.

검색결과 3,457건 처리시간 0.034초

컴퓨터 프로그래밍에 의한 이론적 임계미셀 농도 및 미셀화 영역의 산정에 관한 연구 (Studies on the Estimation of Theoretical cmc and Micellization Range by Computer Programming)

  • 김태영;조경행;최성옥;정노희;남기대
    • 한국응용과학기술학회지
    • /
    • 제14권3호
    • /
    • pp.63-69
    • /
    • 1997
  • 폴리올류 비이온성 계면활성제인 sucrose monolaurate, sucrose monomyristate, sucrose monopalmitate, sucrose monostearate 그리고 sucrose monooleate와 같은 5종의 sucrose monoester 유도체들을 선택하여 묽은 수용액내에서 임계미셀 농도와 미셀화 영역을 결정하는 방법으로서 컴퓨터 프로그래밍에 의한 표면장력 곡선의 최대화를 제안하였다. 임계미셀 농도와 미셀화 영역은 몇몇 실험치를 직접 프로그램에 대입함으로써 곡선의 최대화에 의해서 이론적으로 산정할 수 있다. 이론적으로 구한 임계미셀 농도값들은 실측치와 거의 일치함을 알 수 있다.

Optimization of main factors using response surface method for the enhanced production of hGM-CSF from transgenic Nicotiana tabacum cell suspension cultures

  • Lee, Ki-Yong;Lee, Sang-Yoon;Kim, Dong-Il
    • 한국생물공학회:학술대회논문집
    • /
    • 한국생물공학회 2003년도 생물공학의 동향(XIII)
    • /
    • pp.351-355
    • /
    • 2003
  • Screening 실험을 통하여 결정된 주요인자인 sucrose, nitrogen, 배양온도로 인자의 최적수준을 결정하는 표면반응법중 하나인 Box-Behnken design을 수행하였다. 각 인자의 상관관계를 통하여 sucrose는 90 g/L, nitrogen은 41 mM, 온도는 $22^{\circ}C$가 최적수준으로 결정이 되었으며 확인실험을 통하여 대조구보다 세포생장이 증가하였을 뿐만 아니라 hGM-CSF의 생산도 약 2배 정도 증가되었음을 확인하였다. 이것은 고농도의 sucrose로 인해 배지내로의 hGM-CSF의 분비가 촉진되었기 때문이며 또한 저온으로 인해 분비된 hGM-CSF를 분해하는 protease 활성이 감소되었기 때문인 것으로 사료된다.

  • PDF

Altered Invertase expression induced by BCTV on Arabidopsis

  • Kim, Soyeon;Park, Eunsuk;Lee, Tack-Kyun;Lee, Sukchan
    • 한국식물병리학회:학술대회논문집
    • /
    • 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
    • /
    • pp.74.2-74
    • /
    • 2003
  • Arabidopsis infected with beet curly top virus (BCTV) has the systemic symptoms like stunting of Plant growth, curling of leaves and shoot tips, and callus induction. The regulation of sucrose metabolism by BCTV infection is essential for obtaining the energy source in the process of virus replication and symptom development. Sucrose metabolism-associated gene expression and biochemical enzyme activity were analyzed with the rossette leaves and inflorescencestems of BCTV infected Arabidopsis by the time course of 1, 7, 14, 21 day postinoculation. The expression of invertase and sucrose synthase genes ( encoding sucrose-cleaving enzymes )was increased and reversely the level of Atkin10a ( sucrose non-fermenting gene ) was decreased, resulting by semi-quantitative reverse transcription polymerase chain reaction. The biochemical analysis of invertase and sucrose synthase activity was performed. The activity of neutral invertase in the inflorescence stems was elevated remarkably. The photosynthetic response in the source of sucrose metabolism was consistent with the down-regulation of ribulose 1,5 bisphosphate carboxylase gene, and lower activity than mock-inoculated plants. The levels of genes pertaining to the cell cycle, hormone, and biotic stress-related pathway showed an increase or a decrease dependent on viral symptoms. Therefore, sucrose sensing by BCTV infection can regulate the expression of sucrose metabolism-related key enzymes such as invertase and Atkin10a, and these gene products might influence to symptom development.

  • PDF

Effects of High Level of Sucrose on the Moisture Content, Water Activity, Protein Denaturation and Sensory Properties in Chinese-Style Pork Jerky

  • Chen, W.S.;Liu, D.C.;Chen, M.T.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제15권4호
    • /
    • pp.585-590
    • /
    • 2002
  • The effects of a high level of sucrose on the moisture content, water activity, protein denaturation and sensory properties in Chinese-style pork jerky were investigated. The pork jerky with different levels (0, 12, 15, 18 and 21%) of sucrose was prepared. Fifteen frozen boneless pork legs from different animals were used in this trial. Sucrose is a non-reducing disaccharides and would not undergo non-enzymatic browning. Some studies pointed out that sucrose might be hydrolyzed during freezing, dehydration and storage into glucose and fructose, and cause non-enzymatic browning in meat products. The results showed that moisture content and water activity of pork jerky decreased with increase of the level of sucrose. At the same time, shear value was increased due to the reduced moisture content and water activity by osmotic dehydration. However, a higher level of sucrose had a significantly negative effect on protein solubility and extractability of myosin heavy chain of pork jerky due to non-enzymatic browning. From the results of sensory panel tests, the pork jerky with 21% of sucrose seems to be more acceptable by the panelists in hardness, sweetness and overall acceptability.

보존용액에 함유된 당, 살균제 및 ABA가 절화국화의 수확후 품질에 미치는 영향 (Effect of Sucrose, Germicides and ABA in the Preservatives on Postharvest Quality of Cut Chrysanthemum 'Kyoungsubang`)

  • 박윤영;김학윤;조문수
    • 생명과학회지
    • /
    • 제10권6호
    • /
    • pp.591-597
    • /
    • 2000
  • An investigation was conducted to elucidate the effect of sucrose, germicides and abscisic acid (ABA) in the preservatives on postharvest quality, such as fresh weight, solution uptake, vase life, diameter and thickness of flower, and chlorolhyll of leaf in cut chrysanthemum 'Kyoungsubang`. Fresh weight of cut chrysanthemum was highest in 250mg/L aluminum sulfate [{TEX}$Al_{2}(SO_{4})_{3}${/TEX}]+3% sucrose and 250 mg/L 8-hydroxyquinoline sulfate (8-HQS)+ 3% sucrose. Addition of sucrose and germicides inhibited microorganisms growth in solution, and apparently promoted solution uptake. The increased fresh weight seems to attribute to the elevated uptake. Germicide 8-HQS was more effective than {TEX}$Al_{2}(SO_{4})_{3}${/TEX} in the aspect of solution uptake and fresh weight. Treatment of 250 mg/L 8-HQS +3% sucrose showed the longest vase life (24.67±2.52 days) and the lowest rate of leaf chlorosis (or senescence). Addition of {TEX}$Al_{2}(SO_{4})_{3}${/TEX} and sucrose accelerated leaf chlorosis and increased diameter of flower, respectively. Sucrose treatment prolonged the vase life of flowers. Except control and 250 mg/L 8-HQS, content of chlorophyll was rapidly decreased after 6 days at upper, middle and lower leaves in order.

  • PDF

은나노 소금과 은나노 설탕을 이용한 은나노 입자의 항균 특성 연구 (Antimicrobial Activity of Silver Nanoparticles Attached on the Surface of Salt and Sucrose)

  • 선용호
    • KSBB Journal
    • /
    • 제28권4호
    • /
    • pp.249-253
    • /
    • 2013
  • In this study, we analyzed the attachment amount and the surface properties, such as shape and attachment aspect of silver nanoparticles on the surface of salt and sucrose. In addition, we investigated the antimicrobial activity of silver nanoparticles by measuring total colony counts and total coliforms in sewage according to the time and the amount of silver nanoparticles. As a result, it is found that silver nanoparticles are attached on the surface of salt and sucrose from the SEM images and there is no microorganisms on the surface of salt and sucrose. Silver nanoparticles on salt were rod shape but silver nanoparticles on sucrose were round shape. Also, the content of silver was 0.735 mg/g on salt and 0.885 mg/g on sucrose. In all experiments, total colony counts and total coliforms sharply declined initially, and it decreased gradually as change the time. When injection amount of nano silver sucrose and salt increased, the width of decline was greater. The amount of total colony counts and total coliforms of nano silver salt was much lower and the disinfection efficiency was higher comparing with nano silver sucrose. That means the case of nano silver salt is much better at the antimicrobial activity.

Characteristics of Sucrose Thermal Degradation with High Temperature and High Pressure Treatment

  • Woo, Koan-Sik;Hwang, In-Guk;Lee, Youn-Ri;Lee, Jun-Soo;Jeong, Heon-Sang
    • Food Science and Biotechnology
    • /
    • 제18권3호
    • /
    • pp.717-723
    • /
    • 2009
  • Thermal degradation characteristics of sucrose was investigated. A 20% sucrose solution was heated to temperatures of $110-150^{\circ}C$ for 1-5 hr. Chromaticity, pH, organic acids, 5-hydroxymethylfurfural (HMF), free sugars, electron donating ability (EDA), and ascorbic acid equivalent antioxidant capacity (AEAC) of the heated sucrose solutions were evaluated. With increasing temperatures and times, the L-, a-, and b-values decreased; however, total color difference (${\Delta}E_{ab}$) increased. The pH and sucrose contents decreased, and fructose and glucose contents increased with increasing heating temperature and time. Organic acids, such as formic acid, lactic acid, and levulinic acid, and HMF contents increased with increasing heating temperatures and times. EDA (%) and the AEAC of the heated sucrose solutions increased with increasing heating temperature and time. The heated sucrose solution was more effective than unheated sucrose solution, having higher EDA (90 fold), and AEAC (13 fold).

Membrane Filter와 Sucrose 농도가 도라지(Platycodon grandiflorum A. DC.) 기내 배양묘의 생장에 미치는 영향 (Effects of Membrane Filter and Sucrose Concentrations on the Growth of Balloon Flower (Platycodon grandiflorum A. DC.) Plantlets In Vitro)

  • 최소라;김명준;은종선;안민실;임회춘;류정;유동현
    • Journal of Plant Biotechnology
    • /
    • 제31권3호
    • /
    • pp.209-217
    • /
    • 2004
  • 기내 배양환경을 조절하여 건전한 유식물체를 획득하기 위해 도라지를 무균발아시켜 shoot부분을 NAA 0.1 mg/L가 첨가된 MS 기본배지에 치상한 후 membrane filter 부착 유무와 sucrose 농도를 달리 처리하여 생장반응을 조사하였다. Membrane filter 미부착구에서는 배양용기 내의 $CO_2$$C_2$H$_4$ 농도가 membrane filter 부착구에 비해 높았으며 membrane filter가 부착되지 않았을 때 $CO_2$ 농도는 배양기간이 경과될수록 증가하였으나 $C_2$H$_4$ 농도는 감소하는 경향이었다. 식물체 생장은 membrane filter 부착구가 미부착구에 비해 양호하였고, 미부착구에서는 sucrose 농도가 증가할수록 생육이 양호하게 나타났다. 생체중과 건물중은 membrane filter를 부착하지 않은 sucrose 4.5% 처리구에 비해 membrane filter를 부착한 sucrose 무첨가구에서 비슷하거나 높았다. 엽록소 함량은 membrane filter 부착구에서 미부착구에 비해 높았으며 당 함량도 비슷한 경향이었는데 두 처리간 차이는 더 컸다. 기공은 membrane filter 부착구에서 미부착구에 비해 닫혀 있었으며 엽육조직은 membrane filter를 부착하거나 sucrose 농도가 높을수록 잘 발달하였다. 또한 상토에 이식한 후 13일째 생존율은 sucrose 처리 농도에 관계없이 membrane filter 미부착구는 0%인데 비해 부착구는 100%로 나타났다.

Properties of a New Adhesive Composed of Gambir-Sucrose

  • SUCIPTO, Tito;WIDYORINI, Ragil;PRAYITNO, Tibertius Agus;LUKMANDARU, Ganis
    • Journal of the Korean Wood Science and Technology
    • /
    • 제48권3호
    • /
    • pp.303-314
    • /
    • 2020
  • Gambir is a non-wood forest product with a potential of being used as wood adhesive, due to about 33% catechin in it. Meanwhile, catechins and sucrose have not been studied as adhesives. Therefore, basic characteristics of gambir-sucrose adhesives were investigated. In this research, adhesives were prepared by dissolving gambir and sucrose in distilled water, at different blending ratios of the gambir/sucrose such as 100/0, 75/25, 50/50, and 25/75 wt%. Furthermore, gas chromatography-mass spectrometry (GC-MS) was employed to determine the gambir chemical compositions, and Fourier transform-infrared (FTIR) spectroscopy was carried out to identify chemical bonds. Particleboards with a target density of 0.8 g/㎤ were then manufactured by hot-pressing for 10 min at 200℃. The internal bond (IB) strength of particleboard was subsequently measured. Based on the GC-MS analysis, 31.11% of catechin was identified. In addition, the viscosity, density, solid content, and gelation time of the adhesives, and insoluble matter content (IMC) in boiling water were 7.30~33.24 mPa.s, 1.2~1.3 g/㎤, 25.56~28.44%, 73~420 min, and 29.75~62.10%, respectively. Adding sucrose to the adhesive was observed to raise the IMC from 49.05 to 62.10%, at 180℃ and 200℃. FT-IR analysis showed that the gambir absorption peaks occurred at approximately 1620 cm-1, assigned to the C=O stretching of 5-hydroxymethylfurfural, which tended to increase with the addition of sucrose. The reaction between gambir and sucrose was observed in the form of the dimethylene ether bridge. The 25/75 wt% gambir-sucrose adhesives and 200℃ hot-pressed temperature resulted in the highest IB strength (0.89 MPa), and met the requirement of JIS A5908-2003 type 18. Consequently, the gambir-sucrose adhesive could be used as a particleboard adhesive.

고속액체크로마토그래피에 의한 시판(市販) 수종과실류(數種果實類)의 유리당정량(遊離糖定量) (Determination of Free Sugars in Some Fruits by Liquid Chromatography)

  • 이응호;구재근;이종수;하진환
    • Applied Biological Chemistry
    • /
    • 제27권3호
    • /
    • pp.158-162
    • /
    • 1984
  • 우리나라에서 가을에 수확되는 과실의 가공적성(加工適性)에 대한 자료를 얻을 목적으로 사과, 배, 복숭아, 포도 및 감귤중의 유리당을 HPLC법으로 정향하였다. 감귤류에 있어서 함량이 많은 것은 sucrose, fructose 및 glucose순이었으며, 품종에 따라 유리당함량의 차이가 심하였고, sucrose는 품종에 따라 약간의 차이는 있지만, 총유리당 함량의 약 $53{\sim}65%$를 차지하였다. 복숭아도 감귤과 마찬가지로 함량이 많은 것은 sucrose, fructose, glucose순이었다. 포도와 배의 경우는 fructose, glucose sucrose순으로 함량이 많았고, 특히 장십랑의 fructose함량은 다른 품종보다 2배이상 높았다. 사과의 경우 태양과 홍옥은 fructose, glucose, sucrose순으로 함량이 많았으나, 골덴, 부사, 인도, 육오는 fructose, sucrose, glucose순으로 함량이 많았다.

  • PDF