• BMB Reports
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• 제28권2호
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• pp.184-190
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• 1995

Functional study of the gap junction channel has been hindered by its inaccessibility in situ. Identification of forms of this channel in artificial membrane has been elusive because of the lack of identifying channel physiology. Connexin32 forms gap junction channels between neighboring cells in rat liver. Connexin32 was affinity-purified using a monoclonal antibody and reconstituted into artificial phospholipid vesicles. The reconstituted connexin32 formed channels through the vesicle membrane that were permeable to sucrose (Stokes radius: $5{\AA}$). The permeability to sucrose was reversibly reduced by acidic pH. In addition, the pH effect on the permeability to sucrose fit well with by the Hill's equation (where, n=2.7 and pK=6.7).

• Archives of Pharmacal Research
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• 제19권4호
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• pp.264-268
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• 1996

Panax-ginseng saponin has been known to exert various pharmacological effects on cellular metabolism. This study was performed to determine the effect of ginseng saponin on gap junction channel-mediated intercellular communication, using an established in vitro system of reconstituted gap junction channels. Gap junction channels are a specialized plasma membrane fraction, which are permeable to relatively large water-soluble molecules. The sucrose permeable property of reconstituted gap junction channels was completely inhibited with 0.1 % (w/v) of ginseng saponin. We also compared the effect of ginseng saponin with that of Triton X-100, a nonionic detergent, on the same system. Triton X-100 showed significantly different effect on sucrose-permeability of gap junction channel from that was affected by ginseng saponin. The structures of liposomes containing gap junction channels was significantly destroyed by Triton X-100.

• 멤브레인
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• 제5권2호
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• pp.81-88
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• 1995

NaCl과 sucrose 용액을 역삼투용 워난틀형 모듈에 공겁하여 투과실험하였으며 Kimura-Sourirajan[8]이 제안한 방법으로 분리성능을 분석하였다. 사용한 분리막의 순수투과계수는 압력에 무관하게 $2.17 \times 10^{-6}$(gmol/$cm^2$-sec-bar)로 일정하게 유지되었다. Sucrose 용액의 농도분극화 현상은 NaCl 용액보다 심각하였으며 40bar 이상에서는 압력이 증가함에 따라서 오히려 투과선속이 감소하였다. 용질 배제율은 NaCl 수용액의 경우 압력이 증가함에 따라서 감소하였으나 sucrose 수용액의 경우는 압력에 따라서 배제율도 증가하였다.

• 한국가축번식학회지
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• 제25권2호
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• pp.191-198
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• 2001

본 연구는 체내, 체외수정란 및 배양체계가 세포막투과력 및 GMP vitrification후 생존성에 미치는 영향을 조사하고자 실시하였다. 체내수정란은 6마리 한우를 FSH와 PG $F_{2{\alpha}}$ 에 의한 과배란처리하여 생산하였다. 체외수정란은 난관상피세포 공배양 (OCS) 및 HECM-6 (DCS) 방법으로 생산하였다. 생산된 배반포기 배는 세포력투과력과 GMP vitrification 후 생존성의 조사를 위하여 사용되었다. 세포력투과력은 35$^{\circ}C$ 가온판과 0.5 M sucrose 용액에서 0, 2, 5 및 7분간의 노출시간에 세포질의 “가로 $\times$ 세로”의 직경을 조사하였다. 세포질의 용적은 조사한 직경을 4/3.$\pi$ $r^3$ 공식으로 계산하였다. 배반포의 동결보존은 GMP vitrification 방법으로 실시하였으며, 융해 후 0.25와 0.15 M sucrose 용액 및 TCM199에 각각 5분간 세척한 후 TCM199에 24 또는 48시간동안 배양하였다. 체내수정란의 0, 2, 5 및 7분 때의 용적변화(100, 37.1, 34.3 및 31.6%)는 OCS(100, 59.8, 48.9 및 47.9%)와 DCS(100, 57.2, 47.3 및 46.9%) 보다 유의적으로 높게 수축되었다(P＜0.05). 또한 체내수정란(93.6%)의 동결융해 후 생존성은 OCS 및 DCS (81.9 및 83.6%) 보다 유의적으로 높았다(P＜0.05). 현 배양체계에서 체외수정란의 형태는 체내수정란과 유사하였지만, 세포막투과력 및 응해 후 생존성 등의 질적인 면에서는 큰 차이를 보였다. 결론적으로 세포력 투과력 및 동결융해 후 생존성 등의 질적인 면에서 체내수정란은 OCS 또는 DCS 배양체계에서 생산된 체외수정란보다 우수하였다.

• Journal of Pharmaceutical Investigation
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• 제30권2호
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• pp.99-105
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• 2000

This study compared the permeability of $[^3H]taurine,\;[^3H]phenylalanine,\;and\;[^3H]oxytocin$ through the blood-brain barrier (BBB) in mice and rats with common carotid artery perfusion (CCAP) method that modified internal carotid artery perfusion (ICAP) method. External carotid artery (ECA) was cannulated with coagulating pterygopalatine artery (PPA) in ICAP method, while CCA was cannulated without coagulating PPA in CCAP method. Also, for evaluation of BBB permeability of drugs in mice and rats, we used intravenous injection technique. The results of CCAP method in mice at a perfusion flow-rate of 2 ml/min, the brian volume of distribution $(V_D)$ of $[^{14}C]sucrose,\;[^3H]taurine,\;[^3H]phenylalanine,\;and\;[^3H]oxytocin$ were similar to the result of ICAP method in rats at perfusion flow rate of 4 ml/min. The area under the plasma concentration-time curve and brain uptake of $[^3H]taurine$ by intravenous injection technique, were $65.5{\pm}9.7%ID^*min/ml\;and\;0.515{\pm}0.093%ID/g$, respectively, in mice, and the corresponding values were $8.00{\pm}0.03%ID^*min/ml\;and\;0.052{\pm}0.003%ID/g$ in rats. But the BBB permeability surface-area product of $[^3H]taurine$ was similar between mice and rats. In conclusion, the CCAP method in mice was simple, fast and comparable to ICAP method in rats for drug permeability through the BBB.

• 공업화학
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• 제2권1호
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• pp.64-69
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• 1991

1, 2-Dithiolane 기를 갖는 lipid를 합성하여 vesicle 을 형성시킨 다음 이것을 ring opening polymerization 하여 고분자화된 vesicle을 합성하였다. 중합반응의 반응속도를 UV를 이용하여 측정한 결과, 반응속도가 1차반응 속도식을 만족하였으며 반응속도 상수값은 $3.84{\times}10^{-2}min^{-1}$이었다. 또한 sucrose를 이용한 투과도 실험에서 고분자화된 vesicle의 투과도는 $4.7{\times}10^{-8}cm\;hr^{-1}$이었으며 이 값은 고분자화되지 않은 경우보다 1.5배 낮은 것이다.

• Archives of Pharmacal Research
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• 제23권4호
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• pp.391-395
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• 2000

To establish the usefulness of oxytocin (OT) as an analgesic for women in delivery, the pharmacokinetic parameters and blood-brain barrier (BBB) permeability of [$^3H$] OT were obtained using an intravenous injection technique or the internal carotid artery perfusion/capillary depletion (ICAP/CDM) method. Brain uptake of OT was similar to that of sucrose, plasma space marker, indicating that OT has a poor BBB permeability. Moreover, the analgesic effects of OT injected through the jugular vein on nociception were evaluated by the tail-flick method. The antinociceptive effects of OT injected at a dose of 0.2 ${m}g/kg$or 2 ${m}g/kg$ were dose-dependent. In addition, the analgesic effects of OT on the CNS were unaffected by naloxone, a m-receptor antagonist. In a similar manner to the opioid system, OT may play a modulatory role in antinociception.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2001년도 추계학술대회 및 정기총회
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• pp.90-90
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• 2001

CCAP법을 이용하였을 때 SAM에서 taurine의 뇌 분포용적은 정상생쥐보다 85 % 감소하였다. 그리고 10, 15, 30초 동안의 뇌 분포용적은 linear regression을 나타내었다. 또한 intravenous injection technique의 결과, SAMP8에서의 sucrose space가 정상생쥐에 비하여 감소하였음을 알 수 있었다. 반면에 choline의 경우는 정상생쥐보다 SAMP8의 뇌분포용적이 73% 증가하였다.

• Toxicological Research
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• 제6권1호
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• pp.109-119
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• 1990

Ro09-0198, a cyclic peptide isolated from culture filtrates of Streptoverticillium griseoverticillatum, induced lysis of erythrocytes. Ro-09-0198-induced hemolysis was temperature-dependent and the sensitivity of hemolysis differed greatly among animal species. Preincubation of the peptide with phosphatidylethanolamine reduced the hemolytic activity, whereas other phospholipids present in erythrocytes in nature had no effect. A study of the structural requirements on phosphatidylethanolamine necessary for interaction with the peptide indicates that Ro09-0198 recognizes strictly a particular chemical structure of phosphatidylethanolamine: dialkylphosphoethanolamine as well as 1-acylglycerophosphoethanolamine showed the same inhibitory effct on hemolysis induced by Ro09-0198 as diacylphosphatidyl-ethanolamine, whereas phosphoethanolamine gave no inhibitory effect. Neither phosphatidyl-N-monomethylethanolamine nor alkylphosphopropanolamine had an inhibitory effect. Proton resonances of the peptide were observed in dimethyl sulfoxide solution in the presence of 1-dodecanoyl-sn-glycerophosphoethanolamine. This peptide caused permeability increase and aggregation of liposomes containing phosphatidylethanolamine. A glycerol backbone and a primary amino group of phosphatidylethanolamine are necessary for interaction with Ro09-0198 to cause membrane damage. Ro09-0198 induced a selective permeability change on liposomes. Glucose and umbelliferyl phosphate were effluxed significantly, but sucrose was only slightly permeable and inulin could not be released. Platelet aggregation and serotonin release simultaneously induced by Ro09-0198. Addition of peptide to rat platelet, loaded with the fluorescent $Ca^{++}$ chelator quin-2, caused immediate rise in cytosolic free $Ca^{++}$ to liposomal membrane containing phosphatidylethanolamine was observed dose dependently.

• 한국식품과학회지
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• 제43권3호
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• pp.321-328
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• 2011

본 연구에서는 대추를 필름 형태의 식품 소재로 개발하였다. 대추 필름 제작에 고분자 분쇄 방법으로 사용된 HPH는 대추 필름의 인장 강도와 수분 투과도를 향상시켰다. 기존에 개발된 많은 다른 생고분자 필름보다 높은 인장 강도와 낮은 수분 투과도는 열접합강도와 함께 식품에 있어 HPH 대추 필름의 상업적 적용 가능성을 보여주었다. 대추 필름은 대추 고유의 형태와 크기에 제한 없이 여러 식품에 코팅 또는 롤 형태로 사용될 수 있을 것으로 기대된다.