BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Sucrose-permeability Induced by Reconstituted Connexin32 in Liposomes.

  • Rhee, Senng-Keun (Department of Biochemistry, College of Science, Yeungnam University) ;
  • Hong, Eun-Jnng (Department of Biochemistry, College of Science, Yeungnam University)
  • Received : 1994.11.13
  • Published : 1995.03.31
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Abstract

Functional study of the gap junction channel has been hindered by its inaccessibility in situ. Identification of forms of this channel in artificial membrane has been elusive because of the lack of identifying channel physiology. Connexin32 forms gap junction channels between neighboring cells in rat liver. Connexin32 was affinity-purified using a monoclonal antibody and reconstituted into artificial phospholipid vesicles. The reconstituted connexin32 formed channels through the vesicle membrane that were permeable to sucrose (Stokes radius: $5{\AA}$). The permeability to sucrose was reversibly reduced by acidic pH. In addition, the pH effect on the permeability to sucrose fit well with by the Hill's equation (where, n=2.7 and pK=6.7).

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