• 한국환경독성학회:학술대회논문집
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• 한국환경독성학회 2003년도 춘계학술대회
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• pp.187-187
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• 2003

Methylmercury (MeHg), one of the heavy metal compounds, can cause severe damage to the central nervous system in humans. Many reports have shown that MeHg is poisonous to human body through contaminated foods and has released into the environment. Despite many studies on the pathogenesis of MeHg-induced central neuropathy, no useful mechanism of toxicity has been established so far. In this study, two methods, cDNA Microarray and SSH, were performed to assess the expression profile against MeHg and to identify differentially expressed genes by MeHg in neuroblastoma cell line. TwinChip Human-8K (Digital Genomics) was used with total RNA from SH-SY5Y (human neuroblastoma cell line) treated with solvent (DMSO) and 6.25 uM (IC50) MeHg. And we performed forward and reverse SSH method on mRNA derived from SH-SY5Y treated with DMSO and MeHg (6.25 uM). Differentially expressed cDNA clones were sequenced and were screened by dot blot and ribonuclease protection assay to confirm that individual clones indeed represent differentially expressed genes. These sequences were identified by BLAST homology search to known genes or expressed sequence tags (ESTs). Analysis of these sequences may provide an insight into the biological effects of MeHg in the pathogenesis of neurodegenerative disease and a possibility to develop more efficient and exact monitoring system of heavy metals as environmental pollutants.

• Reproductive and Developmental Biology
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• 제32권2호
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• pp.105-110
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• 2008

The endogenous retrovirus-like elements (HERVs) found on several human chromosomes are somehow involved in gene regulation, especially during the transcription level. HERV-H, located on chromosome Xp22, may regulate gastrin-releasing peptide receptor (GRPR) in connection with diverse diseases. By suppression subtractive hybridization screen on SV40-immortalized lung fibroblast (WI-38 VA-13), we discovered that expression of HERV-HX2, a clustered HERV-H sequence on chromosome X, was upregulated in immortalized lung cells, compared to that of normal cells. Expression of HERV-HX2 was then analyzed in various cell lines, including normal somatic cells, cancer cells, SV40-immortalized cells, and undifferentiated and differentiated human embryonic stem cells. Expression of HERV-HX2 was specifically upregulated in continuously-dividing cells, such as cancer cells and SV40-immortalized cells. Especially, HERV-HX2 in HeLa cells was highly upregulated during the S phase of the cell cycle. Similar results were obtained in hES cells, in which undifferentiated cells expressed more HERV-HX2 mRNA than differentiated hES cells, including neural precursor and endothelial progenitor cells. Taken together, our results suggest that HERV-HX2 is upregulated in cancer cells and undifferentiated hES cells, whereas downregulated as differentiation progress. Therefore, we assume that HERV-HX2 may playa role on proliferation of cancer cells as well as differentiation of hES cells in the transcriptional level.

• 한국산학기술학회논문지
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• 제21권5호
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• pp.103-110
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• 2020

수중 로봇의 가장 기본 성능이라 할 수 있는 동적 성능인 유영속도와 동적 효율 향상을 위해 수중생물을 모사한 로봇들이 주로 연구되고 있다. 그중에서 생체모사 소프트 로봇은 유연한 꼬리지느러미를 적용함으로써 높은 자유도를 구현할 수 있다. 다만, 유연한 구동부의 효율을 높이기 위해서는 구동 주파수에 맞추어 꼬리지느러미의 강성이 바뀌어야 한다. 따라서, 연구를 통해 새로운 형태의 가변강성 메커니즘을 구현하고, 이를 연구 과정에서 검증하였다. 본 연구에서는 실제 돌고래의 해부도에서 영감을 얻어, 가변강성 메커니즘을 적용한 돌고래 로봇을 새로이 설계하고 제작하는 과정을 기술하였다. 실제 돌고래의 척추 모양을 모사하여, 절삭과 적층형 공정으로 가변강성 구동부를 제작하였다. 로봇 돌고래를 구동하기 위한 텐던도 실제 돌고래의 텐던 위치를 고려하여 배치하였으며, 추가로 강성 변화를 위한 텐던을 설치하였다. 돌고래의 유선형 외형을 모사하여 로봇 돌고래를 제작하였고, 강성 변화에 따른 로봇 돌고래의 유영속도를 측정하였다. 동일한 구동 주파수에 꼬리지느러미 구동부의 강성을 변화시켰을 때, 로봇 돌고래의 유영속도의 차이가 약 1.24배, 추력으로는 약 1.5배 변화하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제20권5호
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• pp.768-774
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• 2007

To study the effect of dietary docosahexaenoic acid (DHA) enrichment on the expression of hepatic genes in pigs, weaned, crossbred pigs (30 d old) were fed diets supplemented with either 2% tallow or DHA oil for 18 d. Hepatic mRNA was extracted. Suppression subtractive hybridization was used to explore the hepatic genes that were specifically regulated by dietary DHA enrichment. After subtraction, we observed 288 cDNA fragments differentially expressed in livers from pigs fed either 2% DHA oil or 2% tallow for 18 d. After differential screening, 7 genes were found to be differentially expressed. Serum amyloid A protein 2 (SAA2) was further investigated because of its role in lipid metabolism. Northern analysis indicated that hepatic SAA2 was upregulated by dietary DHA enrichment (p<0.05). In a second experiment, feeding 10% DHA oil for 2d significantly increased the expression of SAA2 (compared to the 10% tallow group; p<0.05). The porcine SAA2 full length cDNA sequence was cloned and the sequence was compared to the human and mouse sequences. The homology of the SAA2 amino acid sequence between pig and human was 73% and between pig and mouse was 62%. There was a considerable difference in SAA2 sequences among these species. Of particular note was a deletion of 8 amino acids, in the pig compared to the human. This fragment is a specific characteristic for the SAA subtype that involved in acute inflammation reaction. Similar to human and mouse, porcine SAA2 was highly expressed in the liver of pigs. It was not detectable in the skeletal muscle, heart muscle, spleen, kidney, lung, and adipose tissue. These data suggest that SAA2 may be involved in mediation of the function of dietary DHA in the liver of the pig, however, the mechanism is not yet clear.

• 한국자원식물학회지
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• 제20권2호
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• pp.216-219
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• 2007

Root colonization by a rhizobacterium, Pseudomonas chlororaphis O6, elicited induced systemic resistance (ISR) in the leaves of cucumber plants against fungal and bacterial pathogens. To understand the role of unique genes during strain O6-mediated ISR, a suppressive subtractive hybridization method was undertaken and led to isolation of twenty-five distinct genes. The transcriptional levels of all the genes showed an increase much earlier under O6 treatment than in water control plants only after challenge with pathogen, while no difference detected on the plants without pathogen challenge. This suggests that O6-mediated ISR is associated with the priming phenomenon, an enhanced capacity for the rapid and effective activation of cellular defense responses after challenge inoculation.

• 전자공학회논문지
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• 제51권5호
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• pp.29-34
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• 2014

본 논문에서는 다중 사용자 다중 안테나 하향링크 채널에서 에너지 효율을 향상시키기 위하여 zero-forcing beamforming (ZFBF) 전처리 필터 기반의 기지국 활성 안테나 수 최적화 기법을 제안한다. 제안하는 기법에서는 최적의 안테나 수를 찾는 연산과정의 복잡도를 줄이기 위하여 사용자의 순시 채널 이득 대신 ZFBF의 평균 채널 이득을 사용한다. 그리고 분수함수 형태의 목적함수를 차를 이용한 목적함수로 변환하여 최적의 안테나 수와 최대 에너지 효율을 찾는 과정을 반복 수행하여 문제를 해결한다. 모의실험을 통해 제안하는 기법의 에너지 효율은 exhaustive search 방법으로 찾은 최대 에너지 효율과 거의 동일함을 확인한다.

• 한국지능시스템학회논문지
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• 제15권6호
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• pp.688-694
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• 2005

본 논문에서는 클러스터링을 뉴로-퍼지 모델에 직접 적용하여 모델을 최적화하는 방법을 제안하였다. 기존의 오차미분기반 학습을 통한 뉴로-퍼지 모델의 최적화 과정과는 달리 제안된 방법은 클러스터링 학습과 연계하여 모델을 구성하며 자율적으로 클러스터의 수를 추정하며 동시에 최적화를 수행한다. 순차적인 학습 기법에서는 각각의 학습 기법을 따로 적용하여 모델링을 실시하였으나 제안된 기법에서는 하나의 클러스터링 학습으로 전체 모델의 학습을 실시하였다. 또한 제안된 방법에서는 클러스터링이 수렴하는 만큼 전체 모델의 연산량이 감소하여 학습과정에서 발생하는 연산량 문제를 개선하였다. 시뮬레이션을 통하여 기존의 연구 결과들과 비교하여 제안된 기법의 유용성을 보였다.

• 전자공학회논문지
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• 제54권5호
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• pp.42-47
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• 2017

본 논문에서는 디지털 영상의 배포에서, 위 변조에 사용되는 미디언 필터링 (Median Filtering : MF)을 분류하는 포렌식 검출 알고리즘을 제안한다. 이러한 문제를 해결하기 위한 특징벡터는 영상의 에지 검출량 정보 32, 64, 128에 대한 허프변환(Hough Transform)에 의하여, 각 허프라인 (Hough Line)의 양끝 좌표값과 Angle-Distance 좌표상의 허프픽크치 (Hough Peaks)를 조합하여 42-Dim.으로 구성하였다. 변조된 영상들 중에서 미디언 필터링을 분류하는 검출기는 SVM (Support Vector Machine)에서 특징벡터를 학습하여 구현되었다. 제안된 미디언 필터링 검출 알고리즘은 특징벡터의 길이가 10-Dim.의 MFR (Median Filtering Residual) 스킴 및 686-Dim.의 SPAM (Subtractive Pixel Adjacency Matrix) 스킴과 비교하여 원영상, 평균필터링 ($3{\times}3$), JPEG (QF=90, 70) 압축, 가우시안 필터링 ($3{\times}3$, $5{\times}5$) 영상 모두에서 미디언 필터링의 포렌식 분류율이 99% 이상의 성능을 확인하였다.

• The Plant Pathology Journal
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• 제21권1호
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• pp.47-54
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• 2005

Identification of host genes involved in disease progresses and/or defense responses is one of the most critical steps leading to the elucidation of disease resistance mechanisms in plants. Soybean mosaic virus (SMV) is one of the most prevalent pathogen of soybean (Glycine max). Although the soybeans are placed one of many important crops, relatively little is known about defense mechanism. In order to obtain host genes involved in SMV disease progress and host defense especially for virus resistance, two different cloning strategies (DD RT-PCR and Subtractive hybridization) were employed to identify pathogenesis- and defenserelated genes (PRs and DRs) from susceptible (Geumjeong 1) and resistant (Geumjeong 2) cultivars against SMV strain G7H. Using these approaches, we obtained 570 genes that expressed differentially during SMV infection processes. Based upon sequence analyses, differentially expressed host genes were classified into five groups, i.e. metabolism, genetic information processing, environmental information processing, cellular processes and unclassified group. A total of 11 differentially expressed genes including protein kinase, transcription factor, other potential signaling components and resistant-like gene involved in host defense response were selected to further characterize and determine expression profiles of each selected gene. Functional characterization of these genes will likely facilitate the elucidation of defense signal transduction and biological function in SMV-infected soybean plants.

• BMB Reports
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• 제38권4호
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• pp.420-431
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• 2005

The differentially virulent race T1 of common bunt (Tilletia tritici) was used to inoculate the wheat lines Neepawa (compatible) and its sib BW553 (incompatible) that are nearly isogenic for the Bt-10 resistance gene. Inoculated crown tissues were used to construct a suppression subtractive hybridization (SSH) cDNA library. Of the 1920 clones arrayed from the SSH cDNA library, approximately 10% were differentially regulated. A total of 168 differentially up-regulated and 25 down-regulated genes were identified and sequenced; 71% sequences had significant homology to genes of known function, of which 59% appeared to have roles in cellular metabolism and development, 24% in abiotic/biotic stress responses, 3% involved in transcription and signal transduction responses. Two putative resistance genes and a transcription factor were identified among the up regulated sequences. The expression of several candidate genes including a lipase, two non-specific lipid transfer proteins (ns-LTPs), and several wheat pathogenesis-related (PR)-proteins, was evaluated following 4 to 32 days post-inoculation in compatible and incompatible interactions. Results confirmed the higher overall expression of these genes in resistant BW553 compared to susceptible Neepawa, and the differential up-regulation of wheat lipase, chitinase and PR-1 proteins in the expression of the incompatible interaction.