• Title/Summary/Keyword: submergence

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Combining In Silico Mapping and Arraying: an Approach to Identifying Common Candidate Genes for Submergence Tolerance and Resistance to Bacterial Leaf Blight in Rice

  • Kottapalli, Kameswara Rao;Satoh, Kouji;Rakwal, Randeep;Shibato, Junko;Doi, Koji;Nagata, Toshifumi;Kikuchi, Shoshi
    • Molecules and Cells
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    • v.24 no.3
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    • pp.394-408
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    • 2007
  • Several genes/QTLs governing resistance/tolerance to abiotic and biotic stresses have been reported and mapped in rice. A QTL for submergence tolerance was found to be co-located with a major QTL for broad-spectrum bacterial leaf blight (bs-blb) resistance on the long arm of chromosome 5 in indica cultivars FR13A and IET8585. Using the Nipponbare (japonica) and 93-11 (indica) genome sequences, we identified, in silico, candidate genes in the chromosomal region [Kottapalli et al. (2006)]. Transcriptional profiling of FR13A and IET8585 using a rice 22K oligo array validated the above findings. Based on in silico analysis and arraying we observed that both cultivars respond to the above stresses through a common signaling system involving protein kinases, adenosine mono phosphate kinase, leucine rich repeat, PDZ/DHR/GLGF, and response regulator receiver protein. The combined approaches suggest that transcription factor EREBP on long arm of chromosome 5 regulates both submergence tolerance and blb resistance. Pyruvate decarboxylase and alcohol dehydrogenase, co-located in the same region, are candidate downstream genes for submergence tolerance at the seedling stage, and t-snare for bs-blb resistance. We also detected up-regulation of novel defense/stress-related genes including those encoding fumaryl aceto acetate (FAA) hydrolase, scramblase, and galactose oxidase, in response to the imposed stresses.

A Study on the Trapezoidal Cutthroat Flumes for Measuring Flow in Open-Channels (개수로의 유량 측정을 위한 제형 Cutthroat Flume에 관한 연구)

  • 윤주상;정하우
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.19 no.4
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    • pp.4533-4543
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    • 1977
  • The purpose of this study was to investigate and compare flow discharges of rectangular, V-notch and trapezoidal type of cutthrooat flumes, and the published data for trapezoidal parshall flumes. And the trapezoidal cutthroat flumes were also compared in their accuracy of discharge measurements for various convergence ratios in the inlet section and divergence ratios in the outlet section. Five flumes were studied, and all the flumes were 45cm long with flat-bottom and were made of well-finished transparent acryl plate of 3mm thickness. One rectangular, one V-notch and three trapezoidal types were numbered 1 to 5 as shown in Fig. III-1. The measured depth of water was ranged from 5 to 20cm. The results obtained in this study are summarized as follows: 1. The general discharge equations for tested prototypes are listed for free flow in Table IV-1 and for submergence flow in Table IV-4. 2. In both free and submerged flow, the accuracy of the discharge formula obtained by this test is highly significant at 1% level as shown in Table IV-2 and Table IV-6. The accuracy of disharges measured depends upon the convergence and divergence ratios in the trapezoidal types: the less the ratios of convergence as well as divergence, the lower the accuracy. 3. Submergence ratios tend to increase in the order of flume number except flume No. 4. This implies that trapezoidal cutthroat flumes are more acceptable than rectangular or V-notch ones for free flow. 4. The transition submergence for the trapezoidal Parshall flumes ranges from 80-85 percent, which is slightly higher than the tested flume. However, the trapezoidal cutthroat flume No. 5 has higher transition submergence ratio, ranging from 73-78 percent, than other trapezoidal ones. The difference between the trapezoidal Parshall flumes and the trapezoidal cutthroat flumes in transition submergence seems small enough to be ignored in their field use. 5. Trapezoidal cutthroat flume is simple and economical to construct in existing openchannels whose shapes are generally trapezoidal. In order to obtain the best rating accuracy, flume No. 3 among the tested trapezoidal types is recommended, because it shows the highest accuracy for both free and submerged flow.

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Pontic site development with an implant submergence technique for unaesthetic implant in the anterior maxilla (상악 전치부 임플란트의 비심미성 개선을 위한 임플란트 침수(submergence)를 동반한 치조제 증대술)

  • Song, Yujeong;Lee, Ju-Youn
    • Journal of Dental Rehabilitation and Applied Science
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    • v.36 no.4
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    • pp.289-295
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    • 2020
  • Improving implant esthetics is very difficult, especially in cases where unaesthetic problems are related to implants in the maxillary anterior dentition. A 69-year old male patient was referred by a prosthodontist for periodic pus discharge and an unaesthetic implant prosthesis (maxillary right lateral incisor). The implant was placed too deeply and showed soft tissue volume deficiency and a long clinical crown. After a clinical and radiographic examination, implant submergence and alveolar ridge augmentation were performed to enhance the aesthetics instead of an explantation. The treatment plan was as follows: extraction the adjacent teeth with tooth mobility, secondary caries, and poor prognosis; placement an additional dental implant with hard and soft tissue grafting; fabrication a fixed bridge using implant abutments. A fixed esthetic prosthesis using implants was fabricated, and the patient was satisfied with the prosthesis. A ridge augmentation with implant submergence may be an alternative for solving the problems of unaesthetic implant restorations in the esthetic zone.

Hydrodynamic Forces Characteristics of a Circular Cylinder with a Damping Plate (감쇠판이 부착된 원기둥의 동유체력 특성)

  • Cho, Il-Hyoung
    • Journal of Ocean Engineering and Technology
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    • v.25 no.1
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    • pp.1-7
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    • 2011
  • The radiation of water waves by a heaving truncated circular cylinder with damping plate is solved in the frame of the three-dimensional linear potential theory. The damping plate has a distinct advantage in reducing the motion response of a floating circular cylinder by increasing the added mass and the damping coefficient. Using the matched eigenfunction expansion method, the characteristics of hydrodynamic added mass and the damping coefficient are investigated with various system parameters, such as the radius and submergence depth of the damping plate. It is found that both added mass and the damping coefficient are significantly increased due to the arranged features of the larger damping plate with shallow submergence, which are positive factors as a motion reduction device of the floating offshore platform. Also the numerical results for an oscillating submerged disk show that the added mass is negative and that the damping coefficient has a peak value at resonant frequency when submergence depth is sufficiently small.

A Prediction of the Air-lift Pump Performance by gas-liquid Flow Charac teristics of Riser (Riser의 기액유동 특성에 따른 Air-lift 펌프의 성능예측)

  • 박찬수
    • Journal of Advanced Marine Engineering and Technology
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    • v.23 no.2
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    • pp.252-258
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    • 1999
  • As an effective means to convey crushed materials from seabed to on board ship and to raise hazardous or abrasive liquids air-lift pump provides a reliable mechanism due to its simple config-uration and easy-to-operate principle. The present study is focused on fundamental investigation of related performance by the analysis program based on the gas-liquid two-phase flow in circular pipes. The program covers pump operating in isothermal and vertical two-phase flow with Newto-nian liquids. it is summarized as important result that an optimum air mass flow rate exists for the maximum lifted liquid mass flow rate in terms of a given submergence rates and furthermore attachment of downcomer gives little effects on riser performance the conveyed liquid flow rate increases with larger submergence rate.

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Genetic interaction of Sub1A and Pup1 in rice

  • Shin, Na-Hyun;Yoo, Soo-Cheul;Chin, Joong Hyoun
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.147-147
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    • 2017
  • Rice is one of the major staple food in Asia, covering around half of the world population. More than 40% of rice cultivation area are subject to abiotic stresses such as drought, submergence and phosphate deficiency. Pyramiding useful genes into elite variety is a promising strategy to develop tolerance varieties to multiple abiotic stresses. However, some genes are not functionally compatible when they are introgressed into the same elite variety. Here, we tested the functional compatibility of Sub1 and Pup1, major QTLs for tolerance to submergence and phosphate (P)-deficiency conditions, respectively. Phenotypic analysis revealed that IR64-Sub1 Pup1(SP1) plants harboring both Sub1 and Pup1 QTLs showed significant tolerance to submerged conditions, similarly in IR64-Sub1 (Sub1) plant, while SP1 plants failed to tolerate to P-deficiency conditions; only IR64-Pup1 (Pup1) showed strong P-deficiency tolerance phenotype. In submerged conditions, the expression levels of Sub1A and PSTOL1, major genes for Sub1 and Pup1 QTLs, respectively, were not significantly different in between Pup1 and SP1 plants. On the other hand, the expression of both Sup1A and PSTOL1 was significantly downregulated in P-deficiency conditions, suggesting that Sub1 and Pup1 repressed gene expression each other in P-deficiency conditions. These results suggest Pup1 does not compromise the Sub1 function in submerged conditions while Sub1 suppresses the function of Pup1 in (P)-deficient condition, possibly by regulating transcript level of Pup1. In conclusion, Sub1 and Pup1 are functionally compatible in terms of submergence tolerance but not in P-deficiency conditions. Further analysis need to be performed to elucidate how Sup1 suppresses the function of Pup1 in P-deficiency conditions.

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A Study on the Propeller Thrust for a Moored Ship (계류중인 선박의 프로펠러 추력 추정에 관한 연구)

  • Ha, M.K.;Song, I.H.;Kim, D.J.;Wee, K.S.;Kim, S.W.
    • Journal of the Society of Naval Architects of Korea
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    • v.34 no.1
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    • pp.50-59
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    • 1997
  • Shipbuilder checks the status of main engine and propeller operation before sea trial. Generally these tests are carried out at a quay during fitting out of the ship. For these tests the operator has to estimate the maximum RPM with permissible torque and thrust to ensure the safety of the mooring line and ship. In this paper, the propeller characteristics according to the draft variation for a moored ship is inveatigated. From these tests, it is shown that shaft submergence is a dominant parameter in the propeller performance at shallow shaft submergence and that the propeller performance is dependent upon the propeller RPM when the shaft submergence is kept unchanged. In this study, a simple formula of the required thrust for a given propeller shaft submergence and propeller RPM is derived. 1be propeller thrust, which is calculated by another formula in case of dtep draft, is compared with results of bollard pull test for FPSO.

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Effect of Free Surface Based on Submergence Depth of Underwater Vehicle

  • Youn, Taek-Geun;Kim, Min-Jae;Kim, Moon-Chan;Kang, Jin-Gu
    • Journal of Ocean Engineering and Technology
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    • v.36 no.2
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    • pp.83-90
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    • 2022
  • This paper presents the minimum submergence depth of an underwater vehicle that can remove the effect of free surface on the resistance of the underwater vehicle. The total resistance of the underwater vehicle in fully submerged modes comprises only viscous pressure and friction resistances, and no wave resistance should be present, based on the free surface effect. In a model test performed in this study, the resistance is measured in the range of 2 to 10 kn (1.03-5.14 m/s) under depth conditions of 850 mm (2.6D) and 1250 mm (3.8D), respectively, and the residual resistance coefficients are compared. Subsequently, resistance analysis is performed via computational fluid dynamics (CFD) simulation to investigate the free surface effect based on various submergence depths. First, the numerical analysis results in the absence of free surface conditions and the model test results are compared to show the tendency of the resistance coefficients and the reliability of the CFD simulation results. Subsequently, numerical analysis results of submergence depth presented in a reference paper are compared with the model test results. These two sets of results confirm that the resistance increased due to the free surface effect as the high speed and depth approach the free surface. Therefore, to identify a fully submerged depth that is not affected by the free surface effect, case studies for various depths are conducted via numerical analysis, and a correlation for the fully submerged depth based on the Froude number of an underwater vehicle is derived.

Oxidative Stress in Rice (Oryza sativa L.) Seedlings Induced by Flooding

  • Lee, Keun Pyo;Jung, Jin
    • Journal of Applied Biological Chemistry
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    • v.44 no.4
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    • pp.159-162
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    • 2001
  • Plant stress incurred by flooding was studied in terms of oxidative stress, using greened rice seedlings subjected to a complete submergence followed by re-exposure to air under illumination ($30W/m^2$). It appeared that shoot tissues of the seedlings suffered oxygen deficiency during the flooding treatment, pertinent to the general concept. Interestingly enough, however, membrane peroxidation in shoots was enhanced by the submergence, as assessed by the content of 2-thiobarbituric acid-reactive substances (TBARS), and the re-aeration resulted in a rapid reduction of TBARS content. Such pattern of response was also seen in the change in the steady state level of $H_2O_2$. In contrast, superoxide dismutase and glutathione reductase that are involved in the detoxifying processes of superoxide in plant cells were significantly activated only during the re-aeration. These results allowed us to suggest the followings as a working hypothesis. Photorespiration-linked production of $H_2O_2$ may largely contribute to the increase in $H_2O_2$ level as well as TBARS production in shoots during the submergence. An abrupt re-supply of $CO_2$ by the re-aeration brings the photosynthetic apparatus back to full operation, suppressing photorespiration and probably causing a momentary, excess formation of superoxide and its dismutation product through side reaction, which gives rise to activating substrate-inducible antioxidative enzymes.

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Thermal Reliability Analysis of the Bearing Units in a Centrifugal Pump (원심펌프 베어링 유닛의 열신뢰성 분석)

  • Moon, Jung-Hwan;Moon, Seung-Jae;Lee, Jae-Heon
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.19 no.4
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    • pp.313-320
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    • 2007
  • In this paper, the experimental study has been carried out to investigate the reliability lifetime of two bearing units based on the oil temperature. Measurements for the oil temperature as well as the bearing temperature during normal operation were performed to study the effects of oil viscosity and oil submergence percentages in the two bearing units. The optimal lubrication condition to increase the lifetime of the bearing unit A was found that its viscosity and submergence percentage were VG32 and 25%, respectively. For the bearing unit B, when the oil viscosity and submergence percent were VG32 and 75%, the lubrication condition was the most efficient. Finally, the adjusted rating times of both the bearing units were calculated to be over 28,000 h, which is greater than the minimum adjusted rating times of 25,000 h. Therefore, they satisfied the regulated lifetime of API 610.