• Title/Summary/Keyword: sub-population

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Evaluation of Salt Tolerance in Sorghum (Sorghum bicolor L.) Mutant Population

  • Ye-Jin Lee;Baul Yang;Woon Ji Kim;Juyoung Kim;Soon-Jae Kwon;Jae Hoon Kim;Joon-Woo Ahn;Sang Hoon Kim;Haeng-Hoon Kim;Chang-Hyu Bae;Jaihyunk Ryu
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2023년도 임시총회 및 춘계학술대회
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    • pp.38-38
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    • 2023
  • Sorghum (Sorghum bicolor L.) is a promising biomass crop with a high lignocellulose content. This study aimed to select high salt-tolerance sorghum lines for cultivation on reclaimed land. Using 7-day seedlings of the sorghum population consisted of 71 radiation-derived mutants (M2 to M6) and 33 genetic resources, survival rate (SR), plant height (PH), root length (RL), fresh weight (FW), and chlorophyll content (CC) were measured for two weeks after 102 mM (0.6%) NaCl treatment. Furthermore, the characteristics of the sorghum population were confirmed using correlation analysis, PCA (principal component analysis), and the FCE (fuzzy comprehensive evaluation) method. Under 102 mM NaCl conditions, SR ranged from 4.9 (IS645-200-6) to 82.4% (KLSo79125-200-1), with an average of 49.9%. PH varied from 7.5 (Mesusu-100-2) to 33.2 cm (DINE-A-MITE-100-2-10), with an average of 20.4 cm. RL ranged from 1.0 (IS645-200-1) to 17.0 cm (30-100-2), with an average of 7.7 cm. FW varied from 0.1 (IS645-200-6) to 4.5 g/plant (DINE-A-MITE-100-2-10), with an average of 2.1 g/plant. CC ranged from 0.9 (DINE-A-MITE-100-2-2) to 3.1 mg/g (IS12937), with an average of 1.7 mg/g. An overall positive correlation, with SR and FW (r = 0.86, P < 0.01), and FW and CC (r = 0.79, P < 0.01), was shown by correlation analysis. Among the five traits, two principal components were extracted by PCA analysis. PC1 was significantly associated with FW, while PC2 was highly involved with RL. To evaluate the salt tolerance level of the sorghum population when an FCE based on trait data was performed, MFV (membership function value) was 0.68. As a result of compiling the MFV of each line, eight lines with MFV > 0.68 were selected. Ultimately, the radiation-derived mutant lines, DINE-A-MITE-100-2-10 and DINE-A-MITE-100-2-12 were selected as salt-tolerant sorghum lines. The results are expected to inform salt-tolerant sorghum breeding programs, and the high salt-tolerance sorghum lines might be advantageous for cultivation on reclaimed land.

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Single nucleotide polymorphism-based analysis of the genetic structure of Liangshan pig population

  • Liu, Bin;Shen, Linyuan;Guo, Zhixian;Gan, Mailing;Chen, Ying;Yang, Runling;Niu, Lili;Jiang, Dongmei;Zhong, Zhijun;Li, Xuewei;Zhang, Shunhua;Zhu, Li
    • Animal Bioscience
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    • 제34권7호
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    • pp.1105-1115
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    • 2021
  • Objective: To conserve and utilize the genetic resources of a traditional Chinese indigenous pig breed, Liangshan pig, we assessed the genetic diversity, genetic structure, and genetic distance in this study. Methods: We used 50K single nucleotide polymorphism (SNP) chip for SNP detection of 139 individuals in the Liangshan Pig Conservation Farm. Results: The genetically closed conserved population consisted of five overlapping generations, and the total effective content of the population (Ne) was 15. The whole population was divided into five boar families and one non-boar family. Among them, the effective size of each generation subpopulation continuously decreased. However, the proportion of polymorphic markers (PN) first decreased and then increased. The average genetic distance of these 139 Liangshan pigs was 0.2823±0.0259, and the average genetic distance of the 14 boars was 0.2723±0.0384. Thus, it can be deduced that the genetic distance changed from generation to generation. In the conserved population, 983 runs of homozygosity (ROH) were detected, and the majority of ROH (80%) were within 100 Mb. The inbreeding coefficient calculated based on ROH showed an average value of 0.026 for the whole population. In addition, the inbreeding coefficient of each generation subpopulation initially increased and then decreased. In the pedigree of the whole conserved population, the error rate of paternal information was more than 11.35% while the maternal information was more than 2.13%. Conclusion: This molecular study of the population genetic structure of Liangshan pig showed loss of genetic diversity during the closed cross-generation reproduction process. It is necessary to improve the mating plan or introduce new outside blood to ensure long-term preservation of Liangshan pig.

Biodegradation of diesel oil and n-alkanes (C18, C20, and C22) by a novel strain Acinetobacter sp. K-6 in unsaturated soil

  • Chaudhary, Dhiraj Kumar;Bajagain, Rishikesh;Jeong, Seung-Woo;Kim, Jaisoo
    • Environmental Engineering Research
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    • 제25권3호
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    • pp.290-298
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    • 2020
  • A large residual fraction of aliphatic components of diesel prevails in soil, which has adverse effects on the environment. This study identified the most bio-recalcitrant aliphatic residual fraction of diesel through total petroleum-hydrocarbon fractional analysis. For this, the strain Acinetobacter sp. K-6 was isolated, identified, and characterized and investigated its ability to degrade diesel and n-alkanes (C18, C20, and C22). The removal efficiency was analysed after treatment with bacteria and nutrients in various soil microcosms. The fractional analysis of diesel degradation after treatment with the bacterial strains identified C18-C22 hydrocarbons as the most bio-recalcitrant aliphatic fraction of diesel oil. Acinetobacter sp. K-6 degraded 59.2% of diesel oil and 56.4% of C18-C22 hydrocarbons in the contaminated soil. The degradation efficiency was further improved using a combinatorial approach of biostimulation and bioaugmentation, which resulted in 76.7% and 73.7% higher degradation of diesel oil and C18-C22 hydrocarbons, respectively. The findings of this study suggest that the removal of mid-length, non-volatile hydrocarbons is affected by the population of bio-degraders and the nutrients used in the process of remediation. A combinatorial approach, including biostimulation and bioaugmentation, could be used to effectively remove large quantities of aliphatic hydrocarbons persisting for a longer period in the soil.

Influence of Different A Elements on Bonding and Elastic Properties of Zr2AC (A = Al, Si, P, S): A Theoretical Investigation

  • Kang, Dae-Bok
    • Bulletin of the Korean Chemical Society
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    • 제34권2호
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    • pp.609-614
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    • 2013
  • Extended H$\ddot{u}$ckel tight-binding band structure calculations are used to address the chemical bonding and elastic properties of $Zr_2AC$ (A=Al, Si, P, and S). Elastic properties are interpreted by analyzing the density of states and the crystal orbital overlap population for the respective phases. Our results show that the bulk modulus of these ternary compounds is determined by the strength of Zr-A bonds.

Genome-wide scans for detecting the selection signature of the Jeju-island native pig in Korea

  • Lee, Young-Sup;Shin, Donghyun;Won, Kyeong-Hye;Kim, Dae Cheol;Lee, Sang Chul;Song, Ki-Duk
    • Asian-Australasian Journal of Animal Sciences
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    • 제33권4호
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    • pp.539-546
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    • 2020
  • Objective: The Jeju native pig (JNP) found on the Jeju Island of Korea is a unique black pig known for high-quality meat. To investigate the genetic uniqueness of JNP, we analyzed the selection signature of the JNP in comparison to commercial pigs such as Berkshire and Yorkshire pigs. Methods: We surveyed the genetic diversity to identify the genetic stability of the JNP, using the linkage disequilibrium method. A selective sweep of the JNP was performed to identify the selection signatures. To do so, the population differentiation measure, Weir-Cockerham's Fst was utilized. This statistic directly measures the population differentiation at the variant level. Additionally, we investigated the gene ontologies (GOs) and genetic features. Results: Compared to the Berkshire and Yorkshire pigs, the JNP had lower genetic diversity in terms of linkage disequilibrium decays. We summarized the selection signatures of the JNP as GO. In the JNP and Berkshire pigs, the most enriched GO terms were epithelium development and neuron-related. Considering the JNP and Yorkshire pigs, cellular response to oxygen-containing compound and generation of neurons were the most enriched GO. Conclusion: The selection signatures of the JNP were identified through the population differentiation statistic. The genes with possible selection signatures are expected to play a role in JNP's unique pork quality.

한국 근해 태평양난바다곤쟁이(Euphausia pacifica)의 유전적 개체군 구조 (Genetic Population Structure of Euphausia pacifica in Korean Waters)

  • 이보람;박원규;지환성;유효재
    • 한국수산과학회지
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    • 제56권5호
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    • pp.701-707
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    • 2023
  • We investigated Euphausia pacifica population in Korean waters in 2016 By samplings for genetic structur at five stations. Three sampling stations were located in the middle of the water masses which were clustered by temperature and salinity whereas the other stations were at the boundaries of the water masses. We amplified a 566 bp region and compared it with sequences of E. pacifica distributed in other waters. Sequences were classified two clades, and a clade was formed in the station E. Genetic distance of station E was close to E. pacifica present in Bering Sea, while it was distant to E. pacifica present in Yellow Sea near China. In genetic analysis, seven haplotypes were formed. Hap-1 and Hap-2 were shared in all five stations, while Hap-3 was shared in station W and WS. Four independent haplotypes were present in station E. Haplotype and nucleotide diversity were the highest in station E and the lowest in station S. The FST distances between station E and other stations were the highest, but distances among other stations were low. As a result, we concluded that E. pacifica, which is distributed in Korean waters, has a genetic population differentiation in the East Sea (station E).

Role of Activated Carbon Modified by H3PO4 and K2CO3 From Natural Adsorbent for Removal of Pb (II) From Aqueous Solutions

  • Manoochehri, Mahboobeh;Khorsand, Ameneh;Hashemi, Elham
    • Carbon letters
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    • 제13권3호
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    • pp.167-172
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    • 2012
  • Most heavy metals are well-known toxic and carcinogenic agents and when discharged into wastewater represent a serious threat to the human population and the fauna and flora of the receiving water bodies. The present study aims to develop a procedure for Pb (II) removal. This procedure is based on using powdered activated carbon, which was prepared from walnut shells that were generated as plant wastes and modified with potassium carbonate and phosphoric acid as chemical agents. The main parameters, such as effect of pH, effect of sorbent dosage, Pb (II) concentrations, and various contact times influence the sorption process. The experimental results were analyzed by using Langmuir, Freundlich, Tempkin, and Dubinin-Radushkevich adsorption models. The kinetic study of Pb (II) on activated carbon from walnut shells was performed based on pseudo- first order and pseudo- second order equations. The data indicate that the adsorption kinetics follow the pseudo- second order rate. The procedure was successfully applied for Pb (II) removal from aqueous solutions.

Role of modified activated carbon by H3PO4 or K2CO3 from natural adsorbent for removal of Pb(II) from aqueous solutions

  • Manoochehri, Mahboobeh;Khorsand, Ameneh;Hashemi, Elham
    • Carbon letters
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    • 제13권2호
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    • pp.115-120
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    • 2012
  • Most heavy metals are well-known toxic and carcinogenic agents and when discharged into wastewater represent a serious threat to the human population and the fauna and flora of the receiving water bodies. The present study aims to develop a procedure for Pb(II) removal. The study was based on using powdered activated carbon, which was prepared from walnut shells generated as plant wastes and modified with potassium carbonate or phosphoric acid as chemical agents. The main parameters, such as effect of pH, effect of sorbent dosage, Pb(II) concentrations, and various contact times influence the sorption process. The experimental results were analyzed by using Langmuir, Freundlich, Tempkin and Dubinin-Radushkevich adsorption models. The kinetic study of Pb(II) on activated carbon from walnut shells was performed based on pseudo-first order and pseudo-second order equations. The data indicate that the adsorption kinetics follow the pseudo-second order rate. The procedure was successfully applied for Pb(II) removal from aqueous solutions.

RE-ACCELERATION MODEL FOR THE 'SAUSAGE' RADIO RELIC

  • KANG, HYESUNG
    • 천문학회지
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    • 제49권4호
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    • pp.145-155
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    • 2016
  • The Sausage radio relic is the arc-like radio structure in the cluster CIZA J2242.8+5301, whose observed properties can be best understood by synchrotron emission from relativistic electrons accelerated at a merger-driven shock. However, there remain a few puzzles that cannot be explained by the shock acceleration model with only in-situ injection. In particular, the Mach number inferred from the observed radio spectral index, Mradio ≈ 4.6, while the Mach number estimated from X-ray observations, MX−ray ≈ 2.7. In an attempt to resolve such a discrepancy, here we consider the re-acceleration model in which a shock of Ms ≈ 3 sweeps through the intracluster gas with a pre-existing population of relativistic electrons. We find that observed brightness profiles at multi frequencies provide strong constraints on the spectral shape of pre-existing electrons. The models with a power-law momentum spectrum with the slope, s ≈ 4.1, and the cutoff Lorentz factor, γe,c ≈ 3−5×104, can reproduce reasonably well the observed spatial profiles of radio fluxes and integrated radio spectrum of the Sausage relic. The possible origins of such relativistic electrons in the intracluster medium remain to be investigated further.

Nuclear energy consumption and CO2 emissions in India: Evidence from Fourier ARDL bounds test approach

  • Ozgur, Onder;Yilanci, Veli;Kongkuah, Maxwell
    • Nuclear Engineering and Technology
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    • 제54권5호
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    • pp.1657-1663
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    • 2022
  • This study uses data from 1970 to 2016 to analyze the effect of nuclear energy use on CO2 emissions and attempts to validate the EKC hypothesis using the Fourier Autoregressive Distributive Lag model in India for the first time. Because of India's rapidly rising population, the environment is being severely strained. However, with 22 operational nuclear reactors, India boasts tremendous nuclear energy potential to cut down on CO2 emissions. The EKC is validated in India as the significant coefficients of GDP and GDP.2 The short-run estimates also suggest that most environmental externalities are corrected within a year. Given the findings, some policy recommendations abound. The negative statistically significant coefficient of nuclear energy consumption is an indication that nuclear power expansion is essential to achieving clean and sustainable growth as a policy goal. Also, policymakers should enact new environmental laws that support the expansion and responsible use of nuclear energy as it is cleaner than fossil fuels and reduces the cost and over-dependence on oil, which ultimately leads to higher economic growth in the long run. Future research should consider studying the nonlinearities in the nuclear energy-CO2 emissions nexus as the current study is examined in the linear sense.