• 대한한의학회지
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• 제27권1호
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• pp.23-35
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• 2006

Objectives : This study was conducted to investigate the inhibitory effects of Gaejibokryunghwan on cell proliferation in HeLa cells. Methods : Human uterine cervical carcinoma HeLa cells were cultured in the 1%, 5% and 10% concentration of Gaejibokryunghwan extract solution. All three were cultured for 24 hours, 48 hours and 72 hours each, to examine the inhibitory effects of Gaejibokryunghwan. Afterwards, we drew out the effect of Gaejibokryunghwan extract solution by making 5 analysis. First analysis was to measure the proliferation rate of cells. Second was FACS analysis. Third was to estimate the activity or caspase-3. Fourth, we used XTT assay to analyze the activation or cells. Ana lastly, a molecular biological method was used to determine activation of MAP kinase in the HeLa cells. Results : After 24, 48 and 72 hours cultivation, the proliferation of HeLa cells showed the dose-dependent decrease in all Gaejibokryunghwan extract solution groups compared to the control group. In the FACS analysis, Gaejibokryunghwan extract solution groups showed increased caspase expression compared to the control group, except for the group for 48 and 72 hours in 1 % concentrate. Caspase-3 activities were increased in all, except tile group cultured for 24 hours in 5% concentrate and the groups cultured for 48 hours in 1% and 5% concentrate. In the XTT study, 1% Gaejibokryunghwan extract solution groups showed increase compared to the control group, but other Gaejibokryunghwan extract solution containing groups showed significant decrease compared to the control after 24, 48 and 72 hours of cultivation. The expressions of MAP kinase were decreased in all Gaejibokryunghwan extract solution containing groups compared to the control group after 24, 48 and 72 hours of cultivation. Conclusions : From this study, we could suggest that Gaejibokryunghwan be available to the inhibition of proliferation of human cervical carcinoma cell line, HeLa cells in vitro.

• 부모자녀건강학회지
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• 제3권1호
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• pp.60-72
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• 2000

Studies are needed to determine the standard norms for sleep/activity patterns in new born baby and there have been no established reports of discrepancy of sleep/activity patterns in new born baby among various races. The purpose of this study is to determine whether the NCASA(Nursing Child Assessment Sleep Activity Record) would be suitable to evaluate and to provide the basic resources for a Korean model of sleep/activity patterns during the new born baby, and to provide a basis for nursing intervention for mothers of new born baby. The subjects of this study were 38 normal new born baby who visited the postpartum care center and two general hospitals located in pusan from January 1 to April 28, 2000. They all agreed to participate in this study. The method of data collection was through convenient sampling. Data were collected through questionnaires including demographic data, birth hi story, and general informations concerning the infant, mother and family. The instrument of this study was the NCASA translated by the Korean parent Child Health Academic Association. The collected data were analysed by mean. standard deviation, frequency, and percentage by use of SPSS/PC. The conclusions obtained from this study are summarized as follows: 1. The mean amount of daytime sleep was 10.23 hours. The mean amount of nighttime sleep was 5.53 hours. The mean amount of total daily sleep was 15.77 hours. The mean of the longest sleep period was 4.49 hours. The mean regularity of daytime sleep was 49.69%. The mean regularity of nighttime sleep was 66.98%. The mean regularity of total daily sleep was 55.81%. The mean frequency of nighttime wakenings was 3.09 times. 2. The mean amount of daytime awake periods was 6.12 hours. The mean amount of nighttime awake periods was 2.11 hours. The mean amount of daily total awake periods was 8.23 hours. The mean of the longest awake periods was 3.76 hours. 3. The mean frequency of daytime feeding was 5.71 times. The mean frequency of nighttime feeding was 2.65 times. The mean frequency of total daily feeding was 8.36 times. The mean frequency regularity of feeding was 62.50%. 4. The mean amount of a mother's day time was 16.36 hours. The mean amount of a mother's night time was 7.64 hours. In conclusion, The new born baby slept more during the daytime compare than nighttime and more active during the daytime. On the other hand. although nighttime sleeping the length of the mother was normal, but sleep was interrupted by the infant over 3 times on average. Therefore this research study will contribute to nursing practice and nursing research by its implication through postnatal educational nursing programs at hospital and nursing intervention programs that would help individual caring of early infant mothers at home.

• 한국보건간호학회지
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• 제11권2호
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• pp.180-193
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• 1997

This study was designed to set up more developed curriculum in Nursing department of a junior college. The sample for the study were fifteen curriculums of fifteen selected junior colleges in Seoul and Kyunggi area. The credits and hours of each curriculum were analyzed into means and compared with the one of the example college. The data were categorized into seven sections; cultural subject, basic medical science, major subjects such as Nursing, Fundamental Nursing, electives, teaching and non-teaching subjects, and clinical practice. The data were analyzed by means of descriptive statistics. The results of the study were as follows; 1. Credit hours of Cultural subjects of each college ranges from $5.8\%\;to\;25.7\%$ of the total graduate credit hours; most commonaly lectured subjects are English 05 colleges), Korean (11 colleges), psychology (10 colleges), and computer (10 colleges). 2. In the case of the example college, Anatomy and Psychology were jointed as one subject, Basic Nursing Science, and some more study in depth should be made to develop more of this type of conjunction among related subjects on basic medical science. 3. As for the mendatory subjects of the example college, subjects on Adult Nursing was fourteen credits (14 hours), which was higher than the average 12.9 hours of other colleges compared. 4. Credit hours of Basic Nursing Laboratory were eight to ten credits (12-18 hours), which was higher than actual class hours. As more and more hospitals test clinical aptitude when recruiting nurses, more emphasis should be paid to the clinical practice. 5. Among fifteen sample curriculums six to twelve electives were offered with twelve to twenty-three credits. Most commonly opened subjects were Physical Examination (5), Nursing English (14). and Geriatrics Nursing (13). Nursing English are considered to be more important in the view of clinical practice. and Oriental Nursing, Nursing Information and Health Insurance Management should be considered as specialized subjects. 6. Teaching and Non-teaching subjects In case of the example school. Clinical Emergency Medicine, Introduction to Emergency Medicine were offered for these non-teaching class students so they could prepare for the qualification examination. 7. Clinical Practice The average credit hours for clinical practice of the sample college were 20.9 credit hours $(66.5\%)$ and the example school offered twenty credit hours which was slightly lower than the other forteen.

• 수산해양기술연구
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• 제58권3호
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• pp.205-213
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• 2022

The experimental fish transplanted from China in 2015 was used after seedling production and cultivated. Breeding management for experiment was carried out from October 2020 to February 2021. Also, it succeeded in inducing artificial maturation three to four months earlier than wild broodstock and secured good quality fertilized eggs. The average size of fertilized eggs was 1.22 mm, at 20℃ Blastodisc (15 minutes post-fertilization), 2 cell (50 minutes), 4 cell (1 hours), 8 cell (2 hours), 16 cell (2 hours and 30 minutes), 32 cell (2 hours and 50 minutes), morula (3 hours), blastula (8 hours), gastrula (15 hours), skull formation (20 hours), organ formation (30 hours) and hatching yolk larvae stage (35 hours). The total length of the just hatched larvae were 2.50 ± 2 mm, and then gain growth of 42.5 mm by 60 days, reaching 45 ± 5 mm.

• 한국가축번식학회지
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• 제16권1호
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• pp.21-38
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• 1992

The present study was carried out to investigate morphologic changes in the corpus luteum of the pregnant rat by electron microscope after administration of prostaglandin F2$\alpha$(PGF2$\alpha$). Pregnant rates were treated with PGF2$\alpha$(1,500$\mu\textrm{g}$/rat) and their corpura lutea were observed morphologically. The results obtained in this study were summarized as follows ; 1. The weight of the ovaries and corpura lutea were decreased slightly at 8~24 hours after PGF2$\alpha$ administratin but no significant differences were observed. 2. The number of corpora lutea and luteal cells decreased slightly at 12~48 hours and 18~24 hours after PGF2$\alpha$ tretment but there were no signifciant differences between control and treatment. 3. The weight of uterus and the unmber of embryo decreased slightly at 96 hours and at 18~96 hours after PGF2$\alpha$ administration but no significant differences were obtained. 4. In the electron microscopic observatons, lipid droplets which are electron dense and appear in the cytoplasm moderately increased in number after PGF2$\alpha$ treatment. The lipid droplets were surrounded by mitochodria and appeared in the autophagic vacuoles. 5. Moderated and high electron dense mitochondria which are round or elongated in shape showed pleomorphism from 3 hours after PGF2$\alpha$ treatment. Destruction of tubular of vesicular cristae was observed at 6 hours after the treatment. Dense body and myelin figures in matrix of mitochondria were also appeared. 6. Well-developed smooth endoplasmic reticulum(sER) showed tubular or vesicular cisternae. A number of whorl membranes containing ribosomes, mitochondria and lipid droplets were observed at 1.5 hour after treatment. sER was abundant in luteal cells at 12 hours were treatment. 7. Well-developed Golgi pparatus appeared obviously 6 hours and more prominently at 12 hours. Those Golgi vesicles were remarkably dilated. 8. Generally, a few rough endoplasmic reticulum (rER) were appeared after treatment and cisternae showed slight dilatation. No differences among the treatments were observed. However, slight dilation of cisternae was observed at 1.5 hours after treatment. 9. Ribosomes composed of free and polyribosomes were abundant before treatment but polyribosomes were appeared at 12 to 24 hours after treatment. 10. Intercellular space were slightly extended at 3 hours and markedly extended at 12 hours. Numerous microvillous protrusions were observed at these times. Membranous multivesicular structures and autophagic vacuoles were also appeared in the intercellular space. 11. At 3 hours after the treatment, autophagic vacuoles appeared in the cytoplasm of the cell. They increased in number with time and were observed to transfer to the intercellular space. Lysosomal dense body appeared in the cytoplasm and the inclusion body was also observed in nucleus at 12 to 24 hours after treatment.

• 한국정보교육학회:학술대회논문집
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• 한국정보교육학회 2021년도 학술논문집
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• pp.107-112
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• 2021

본 연구에서는 초등학교에서 정보교육을 하기 위해 얼마나 시수가 필요할지 알아보기 위해 서울 소재 사립초등학교의 정보교육 시수를 분석해보고자 하였다. 최근 실시된 2022개정교육과정 온라인 토론참여 및 조사결과 안내에 따르면 학생, 학부모가 정보교육이 강화되기를 원하는 것으로 나타났다. 현재 공립초등학교에서의 정보교육은 2015개정교육과정을 기준으로 실과교과에 별도 단원으로 구성되어 17시간 이상을 실시하게 되어 있다. 서울에 있는 사립초등학교 28개의 평균 정보교육 시수를 조사한 결과 6년 동안 약 152시간의 정보교육시수를 운영하고 있는 것으로 조사되었다. 이는 공립초등학교와 약 9배정도의 교육시수 격차가 발생한 것이다. 인공지능이 중요하게 대두되고 있는 현대 사회에서 정보교육시수는 학생들의 미래역량강화를 위해 꼭 확보되어야 한다. 따라서 초등학교급에서도 정보교육을 하나의 교과로 독립하고 시수를 확보하여야 할 것이다.

• Journal of Korean Biological Nursing Science
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• 제17권3호
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• pp.236-244
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• 2015

Purpose: The study was conducted to identify factors affecting the intraoperative core body temperature (CBT) of surgical patients under general anesthesia. Methods: This study was performed through a prospective descriptive research design. The sample consisted of 138 patients who had undergone elective laparotomy surgery. Age, weight, height, the basal preoperative CBT, blood pressure, and heart rate were collected. CBT was again measured at induction of anesthesia, 1 hour, 2 hours, and 3 hours following induction of general anesthesia. Results: Factors affecting intraoperative hypothermia < $36^{\circ}C$ at 1 hour following induction, were CBT at induction and total body fat (TBF) ($R^2=.569$, p<.001); at 2 hours after induction, CBT at induction and TBF ($R^2=.507$, p<.001); at 3 hours after induction, CBT at induction (${\beta}=0.34$), TBF, age and the ambient temperature in the operating room ($R^2=.449$, p<.001). Conclusion: CBT at induction and TBF appear to be factors affecting intraoperative CBT within 2 hours after induction of anesthesia; CBT at induction, TBF, advanced age and the ambient temperature after 3 hours following induction. We recommend keeping surgical patients warm before induction of anesthesia and providing intraoperative warming for surgical patients of advanced age with low TBF and when the duration of general anesthesia will last more than 3 hours.

• Safety and Health at Work
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• 제13권1호
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• pp.17-22
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• 2022

Background: This study aimed to identify work-related risk factors, including long working hours and night/shift work, for unmet health care need using data of a representative panel of Korean adults. Methods: Associations between work-related factors and unmet health care need were analyzed using data of 3,440 participants (10,320 observations) from the 2011-2013 Korean Health Panel Study. A generalized estimating equation was used for the analysis of repeated measures. Results: The prevalence of unmet health care was 16.6%. After adjusting sex, age, socioeconomic status, work characteristics, and working more than 60 hours per week (odds ratio [OR]: 1.43, 95% confidence interval [CI]: 1.23-1.65) or 50-59 hours per week (OR: 1.26, 95% CI: 1.08-1.46) instead of 40-49 hours per week and night/shift work (OR: 1.27, 95% CI: 1.06-1.51) were associated with unmet health care need. Conclusion: Long working hours and night/shift work are risk factors for unmet health care need among the Korean working population.

• Safety and Health at Work
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• 제14권1호
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• pp.59-65
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• 2023

Objectives: Epidemiological evidence linking long working hours and shift work to metabolic syndrome remains inadequate. We sought to evaluate the impact of reducing working hours on metabolic syndrome. Methods: We compared the prevalence of metabolic syndrome among male manual workers in a manufacturing company (N = 371) before and after the introduction of policy to reduce daily work hours from 10 to 8 hours. Components of metabolic syndrome were measured in periodic health examinations before the intervention, 6-9 months after, and 1.5-2 years after the intervention. Generalized estimating equation models were used to estimate changes in the prevalence of metabolic syndrome. Analyses were stratified by day work versus shift work. Results: The results showed a significantly decreased prevalence of metabolic syndrome 6-9 months following the intervention in day workers (risk ratio = 0.68, 95% confidence interval 0.52-0.88), but the benefit disappeared after 1.5-2 years. Shift workers showed a decreased prevalence of metabolic syndrome for the whole follow-up duration after the intervention, although the change was not statistically significant. Conclusion: Reducing working hours was associated with short-term improvement in metabolic syndrome in male manual workers.

• 대한간호학회지
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• 제14권1호
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• pp.60-68
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• 1984

The main purpose of this study is to observe patterns of pain of surgical patients following surgery The postoperative pain was checked with the interval of every 2 hours from 6 hours to 80 hours after surgery. Graphic rating scale from unidimensional concept of pain and sensory intensity scale and unpleasantness scale from two dimensional concept of pain were used for pain measurement. Thirty two patients were participated in this study in which 22 were undergone upper abdominal surgery, 7 thyroid or neck surgery and 3 other surgeries. The findings obtained from this study were as follows: 1) In all cases of using 3 different pain measurement tools, postoperative pain was markedly decreased since 36 hours after surgery. In case of patient's less cooperation, either sensory intensity scale or graphic rating scale may be chosen for the measurement of pain. 2) Pain amounts measured by sensory intensity scale were highly correlated with those measured by unpleasantness scale in all situations except several situations having few cases included. Unpleasantness scale may be separately used for themeasurement of affective response due to pain. 3) Almost 90% of total amount of analgesics used for relief of pain were used within 36 hours after surgery. 4) Mean frequency of analgesics used by every patient during 80 hours following surgery was 0.84.