• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.20 no.3
• /
• pp.168-175
• /
• 2019

The purpose of this paper is to examine the characteristics of organic relations according to the characteristics of space and place in which human activities are spreading. The diffusion of various human experiences and the interaction of space and place that form a mutual construction network create momentum for expanding individual or collective experiences. The space and place that lead to the interactions of human activities are expressed in various ways. Through the domestic travel program especially, the ways of expressing space and place are spreading as various "fields of experience." The composition and the expression system of a travel program is transformed into a subject of thought, not a mere experience of space and place through the formation of a "field of experience" that can be thought out and thought of as an existing travel style. Therefore, in this study, we examine the characteristics of the MBC Plus channel from a socio-cultural perspective, expressed through the experience of space and place characteristics expressed through a travel program. The result of an analysis of story development composition for this study shows that the experience of space and place minimizes the boundaries of various cultural discounts according to their relationships to food and communication. In addition, the analysis results of a three-way communication structure show that the emotional code of the audience is maximized through the process of maximizing the effect of reality, and minimizing the gap between the cultural differences and the boundaries through the empathic situation and the empathic subject.

• Korean Journal of Animal Reproduction
• /
• v.26 no.3
• /
• pp.299-308
• /
• 2002

In horse, a single gene encodes both eCG and eLH $\beta$ subunits. The difference between eCG and eLH lies in the structure of their glycoresidues, which are both sialylated and sulfated in LH and sialylated in CG eCG consists of highly glycosyiated $\alpha$- and $\beta$-subunits and is an unique member of the gonadotropin family because it elicits response characteristics of both FSH and LH in other species than the horse. This dual activity of eCG in heterologous species is of fundamental interest to the study of gonadotropin structure-function relationships and the understanding of the molecular bases of the specific interactions of these hormones with their receptors. Thus, eCG is a dintinct molecule from the view points of its biological function and glycoresidue structures. The oligosaccharide at Asn 56 of the $\alpha$-subunit plays an indispensable role, whereas the carboxyl-terminal extension of the eCG $\beta$-subunit with its associated O-linked oligosaccharides is not improtant for, the in vitro LH-like activity of eCG. In contrast, both N- and O-linked oligosaccharides play important roles for FSH-like activity and increase FSH-like activity by removal of N- and O-linked oligosaccharides. Therefore, the dual LH- and FSH-like activities of eCG can be clearly separated by removal of either the N-linked oligosaccharide on the $\alpha$-subunit or CTP-associated O-linked oligosaccharides from its $\beta$-subunit. The glycoresidues seem to play crucial roles fer biological activities. The tethered-eCG was effciently secreted and showed similar LH-like activity to the dimeric eCG $\alpha$/ $\beta$ and native eCG. FSH-like activity of the tethered-eCG was also shown similarly in comparison with the native and wild type eCG $\alpha$/ $\beta$. Our data for the first time suggest that the tethered-eCG can be expressed efficiently and the produced product by the CHO-Kl cells is fully LH- and FSH-like activities in rat in vitro bioassay system. Our results also suggest that this molecular can imply particular models ot FSH-like activity not LH-like activity in the eCG. Taken together, these data indicate that the constructs of tethered molecule will be useful in the study of mutants that affect subunit association and/or secretion.

• Proceedings of the KSAR Conference
• /
• 2000.10a
• /
• pp.10-12
• /
• 2000

Members of the glycoprotein family, which includes CG, LH, FSH and TSH, comprise two noncovalently linked $\alpha$- and $\beta$-subunits. Equine chorionic gonadotropin (eCG), known as PMSG, has a number of interesting and unique characteristics since it appears to be a single molecule that possesses both LH- and FSH-like activities in other species than the horse. This dual activity of eCG in heterologous species is of fundamental interest to the study of the structure-function relationships of gonadotropins and their receptors. CG and LH $\beta$ genes are different in primates. In horse, however, a single gene encodes both eCG and eLH $\beta$-subunits. The subunit mRNA levels seem to be independently regulated and their imbalance may account for differences in the quantities of $\alpha$ - and $\beta$ -subunits in the placenta and pituitary. The dual activities of eCG could be separated by removal of the N-linked oligosaccharide on the $\alpha$-subunit Asn 56 or CTP-associated O-linked oligosaccharides. The tethered-eCG was. efficiently secreted and showed similar LH-like activity to the dimeric eCG. Interestingly, the FSH-like activity of the tethered-eCG was increased markedly in comparison with the native and wild type eCG. These results also suggest that this molecular can implay particular models of FSH-like activity not LH-like activity in the eCG/indicate that the constructs of tethered molecule will be useful in the study of mutants that affect subunit association and/or secretion. A single-chain analog can also be constructed to include additional hormone-specific bioactive generating potentially efficacious compounds that have only FSH-like activity. The LH/CG receptor (LH/CGR), a membrane glycoprotein that is present on testicular Leydig cells and ovarian theca, granulosa, luteal, and interstitial cells, plays a pivotal role in the regulation of gonadal development and function in males as well as in nonpregnant and pregnant females. The LH/CGR is a member of the family of G protein-coupled receptors and its structure is predicted to consist of a large extracellular domain connected to a bundle of seven membrane-spanning a-helices. The LH/CGR phosphorylation can be induced with a phorbol ester, but not with a calcium ionophore. The truncated form of LHR also was down-regulated normally in response to hCG stimulation. In contrast, the cell lines expressing LHR-t63I or LHR-628, the two phosphorylation-negative receptor mutant, showed a delay in the early phase of hCG-induced desensitization, a complete loss of PMA-induced desensitization, and an increase in the rate of hCG-induced receptor down-regulation. These results clearly show that residues 632-653 in the C-terminal tail of the LHR are involved in PMA-induced desensitization, hCG-induced desensitization, and hCG-induced down-regulation. Recently, constitutively activating mutations of the receptor have been identified that are associated with familial male-precocious puberty. Cells expressing LHR-D556Y bind hCG with normal affinity, exhibit a 25-fold increase in basal cAMP and respond to hCG with a normal increase in cAMP accumulation. This mutation enhances the internalization of the free and agonist-occupied receptors ~2- and ~17-fold, respectively. We conclude that the state of activation of the LHR can modulate its basal and/or agonist-stimulated internalization. Since the internalization of hCG is involved in the termination of hCG actions, we suggest that the lack of responsiveness detected in cells expressing LHR-L435R is due to the fast rate of internalization of the bound hCG. This statement is supported by the finding that hCG responsiveness is restored when the cells are lysed and signal transduction is measured in a subcellular fraction (membranes) that cannot internalize the bound hormone.

• Korean Journal of Animal Reproduction
• /
• v.24 no.4
• /
• pp.357-364
• /
• 2000

Members of the glycoprotein family, which includes CG, LH, FSH and TSH, comprise two noncovalently linked $\alpha$- and $\beta$-subunits. Equine chorionic gonadotropin (eCG), known as PMSG, has a number of interesting and unique characteristics since it appears to be a single molecule that possesses both LH- and FSH-like activities in other species than the horse. This dual activity of eCG in heterologous species is of fundamental interest to the study of the structure-function relationships of gonadotropins and their receptors. CG and LH $\beta$ genes are different in primates. In horse, however, a single gene encodes both eCG and eLH $\beta$ -subunits. The subunit mRNA levels seem to be independently regulated and their imbalance may account for differences in the quantities of $\alpha$ - and $\beta$-subunits in the placenta and pituitary. The dual activities of eCG could be separated by removal of the N-linked oligosaccharide on the $\alpha$-subunit Asn 56 or CTP-associated O-linked oligosaccharides. The tethered-eCG was efficiently secreted and showed similar LH-like activity to the dimeric eCG. Interestingly, the FSH-like activity of the tethered-eCG was increased markedly in comparison with the native and wild type eCG. These results also suggest that this molecular can implay particular models of FSH-like activity not LH-like activity in the eCG/indicate that the constructs of tethered molecule will be useful in the study of mutants that affect subunit association and/or secretion. A single-chain analog can also be constructed to include additional hormone-specific bioactive generating potentially efficacious compounds that have only FSH-like activity. The LH/CG receptor (LH/CGR), a membrane glycoprotein that is present on testicular Leydig cells and ovarian theca, granulosa, luteal, and interstitial cells, plays a pivotal role in the regulation of gonadal development and function in males as well as in nonpregnant and pregnant females. The LH/CGR is a member of the family of G protein-coupled receptors and its structure is predicted to of a large extracellular domain connected to a bundle of seven membrane-spanning a-helices. The LH/CGR phosphorylation can be induced with a phorbol ester, but not with a calcium ionophore. The truncated form of LHR also was down-regulated normally in response to hCG stimulation. In contrast, the cell lines expressing LHR-t631 or LHR-628, the two phosphorylation-negative receptor mutant, showed a delay in the early phase of hCG-induced desensitization, a complete loss of PMA-induced desensitization, and an increase in the rate of hCG-induced receptor down-regulation. These results clearly show that residues 632~653 in the C-terminal tail of the LHR are involved in PMA-induced desensitization, hCG-induced desensitization, and hCG-induced down-regulation. Recently, constitutively activating mutations of the receptor have been identified that are associated with familial male-precocious puberty. Cells expressing LHR-D556Y bind hCG with normal affinity, exhibit a 25-fold increase in basal cAMP and respond to hCG with a normal increase in cAMP accumulation. This mutation enhances the internalization of the free and agoinst-occupied receptors ~2- and ~17- fold, respectively. We conclude that the state of activation of the LHR can modulate its basal and/or agonist-stimulated internalization. Since the internalization of hCG is involved in the termination of hCG actions, we suggest that the lack of responsiveness detected in cells expressing LHR-L435R is due to the fast rate of internalization of the bound hCG. This statement is supported by the finding that hCG responsiveness is restored when the cells are lysed and signal transduction is measured in a subcellular fraction (membranes) that cannot internalize the bound hormone.

• Korean journal of communication and information
• /
• v.51
• /
• pp.5-25
• /
• 2010

The purpose of this research examined the relationship between the level of news usage on Influenza A(H1N1) and media users' behavioral intention toward health protection. Specifically, the study investigated causal relationships among exposure of media information on Influenza A(H1N1) as an independent variable, psychological protection motivation factors such as perceived severity, vulnerability, response efficacy, & self-efficacy as mediated variables and behavioral intention on personal and public health protection as dependent variables using structure equation model analysis. Self-reported questionnaires were administrated to 460 college students. The result indicated the followings. First, the level of news usage on Influenza A(H1N1) was not statistically significant on influencing behavioral intention toward personal and public health protection. Second, perceived severity, vulnerability, and self-efficacy were significant variables on behavioral intention toward personal health protection whereas perceived vulnerability and response efficacy were significant on public health protection. The function among factors in protection motivation theory is dependent upon whom protected from illness.

• Korean Journal of Ecology and Environment
• /
• v.51 no.1
• /
• pp.40-59
• /
• 2018

This review summarizes the history of freshwater zooplankton research in Korean Society of Limnology and necessary future topics that are remain poorly investigated in South Korea based on main research topics of published articles focusing on lakes, reservoirs, rivers and wetland ecosystems. In Korea, a total 450 freshwater zooplankton species have been reported (85 species of cladocera, ca. 230 species of rotifera since 1939, and 138 species of copepoda), and they cover 10% of total zooplankton species list. In the present paper, we provide recent species list of zooplankton found in Korea and their taxonomic keys. Over periods of 45 years, there are 25 published papers for zooplankton biota in lentic ecosystems in Korean Journal of Ecology and Environment (KJEE). The ecology of zooplankton communities in rivers has focused on the mechanisms involved in regulating their abundance, diversity and spatio-temporal patterns of genus Bosmina and rotifers (genus Brachionus and Keratella) that are most frequently found from Korea. On the other hand, the studies on zooplankton in wetland has focused on Alona, Chydorus and Ceriodaphnia with special emphasis on their relationships with aquatic macrophytes. Even though studies on the freshwater ecosystem in Korea have a long history, a few of studies on zooplankton biota were conducted at rice paddy, Dumbeong and wetlands. During the last two decades, experimental advances in freshwater zooplankton ecology and understanding of structure and function of this biota were made from a series of articles mainly in journal of KJEE. For future studies, quantitative, experimental and interdisciplinary approaches would be key words to understand zooplankton ecology and their roles in aquatic ecosystems under changing environments, and we have suggested necessary zooplankton research fields and future directions.

• Applied Biological Chemistry
• /
• v.38 no.2
• /
• pp.118-122
• /
• 1995

Escherichia Coli ornithine transcarbamylase is the enzyme which catalyzes the L-citrulline biosynthesis from L-ornithine and carbamyl phosphate. To facilitate the purification of enzyme which will be used for many biochemical studies such as structure and function relationships and catalytic mechanisms, the cloning and expression of E. coli argI gene for ornithine transcarbamylase was conducted. argI was amplified from genomic DNA of E. coli strain of $DH5{\alpha}$, by polymerization chain reaction (PCR) method. The amplified argI gene was ligated to the prokaryotic expression vector pKK223-3 and used for transformation of E. coli TB2 which was deficient of ornithine transcarbamylase. The over-produced enzyme by the tnansformant was purified by ammonium sulfate fractionation, heat denaturation and affinity chromatography. The result of SDS denaturation gel electrophoresis for the purified enzyme showed a single band of about 38 kDa of ornithine transcarbamylase. Kinetic data for the expressed enzyme gave almost the s?????? values as those of the wild type enzyme. The $k_{cat}$, of the enzyme was $1.0{\times}10^5min^{-1}$, and $K_ms$ for ornithine and carbamyl phosphate were 0.35 mM and 0.06 mM, respectively.

• Applied Biological Chemistry
• /
• v.38 no.2
• /
• pp.163-167
• /
• 1995

New twenty 2-(n-octyl)-3-(n-propyl)isothiourea derivatives(S) were synthesized which is modified from the insecticidal Buprofezine (Applaud) in the selective insecticidal activities in-vitro against Diamond-Back moth (Plutella Xylostella Linnaeus). The structure activity relationships(SAR) between the insecticidal activity$(pI_{50})$ and a various physicochemical parameters of the substituent(Z) of S were analyzed by the multiple regression technique. The activities would depend largely on the MR, ${\pi}$ and $L_1$ parameters. The SAR was rationalized by parabolic function of MR, ${\pi}$ and $L_1$ constant, where the optimal values of the constants were $L_1=5.22{{\AA}}$, $MR=15.70\;Cm^3/mol$ and ${\pi}=1.60$, respectively. The steric effects play an important role in determining insecticidal activity. The SAR suggest that the S derivatives having a substituents with a small breadth and an appropriate length as Z group showed potent activity. From the results, the iso-propyl group(Z) substituent (5) with three carbon atom was the most effective compound.

• The Journal of the Korea Contents Association
• /
• v.13 no.7
• /
• pp.463-472
• /
• 2013

The purpose of this study is to examine the relationships among three factors such as the family health, school adjustment and internet addiction, and their subordinated factors. The research is going to clarify if school adjustment has mediating effect in the relationship between the family health and internet addiction. 371 of elementary school students resideing in Dadjeon-city completed questionnaires assessing family health, school adjustment and internet addiction. The sample variance-covariance matrix was analyzed using SPSS 18.0 and AMOS 20.0, the mazimum likelihood minimization function. The goodness of fit was evaluated using the SRMR, RMSEA and its 90% confidence interval, CFI, and TLI. The results are as follows: First, there were significant correlations among family health, school adjustment and internet addiction. Second, the subordinated factors in the family health, school adjustment, and internet addiction regulation are also closely related in the statistically significant positive relationship excepting school adjustment. After conducting the Covariance Structure Analysis to verify the mediating effect of school adjustment in the relationship between the family health and internet addiction, we found that it was appropriate to explain our findings with the structural equation model. when the family health has an effect on internet addiction, the school adjustment partially influences this process. Finally, educational implications and suggestions for future studies are addressed.

• Journal of Korea Water Resources Association
• /
• v.51 no.9
• /
• pp.769-782
• /
• 2018

Estimation of design floods is typically required for hydrologic design purpose. Design floods are routinely estimated for water resources planning, safety and risk of the existing water-related structures. However, the hydrologic data, especially streamflow data for the design purposes in South Korea are still very limited, and additionally the length of streamflow data is relatively short compared to the rainfall data. Therefore, this study collected a large number design flood data and watershed characteristics (e.g. area, slope and altitude) from the national river database. We further explored to formulate a scaling approach for the estimation of design flood, which is a function of the watershed characteristics. Then, this study adopted a Hierarchical Bayesian model for evaluating both parameters and their uncertainties in the regionalization approach, which models the hydrologic response of ungauged basins using regression relationships between watershed structure and model. The proposed modeling framework was validated through ungauged watersheds. The proposed approach have better performance in terms of correlation coefficient than the existing approach which is solely based on area as a predictor. Moreover, the proposed approach can provide uncertainty associated with the model parameters to better characterize design floods at ungauged watersheds.