• Title/Summary/Keyword: strips

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Two-dimensional gel Electrophoresis of Helicobacter pylori for Proteomic Analysis

  • Jung, Tae-Sung;Kang, Seung-Chul;Choi, Yeo-Jeong;Jeon, Beong-Sam;Park, Jeong-Won;Jung, Sun-Ae;Song, Jae-Young;Choi, Sang-Haeng;Park, Seong-Gyu;Choe, Mi-Young;Lee, Byung-Sang;Byun, Eun-Young;Baik, Seung-Chul
    • The Journal of the Korean Society for Microbiology
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    • v.35 no.2
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    • pp.97-108
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    • 2000
  • Two-dimensional gel electrophoresis (2-DE) is an essential tool of proteomics to analyse the entire set of proteins of an organism and its variation between organisms. Helicobacter pylori was tried to identify differences between strains. As the first step, whole H. pylori was lysed using high concentration urea contained lysis buffer [9.5 M Urea, 4% CHAPS, 35 mM Tris, 65 mM DTT, 0.01% SDS and 0.5% Ampholite (Bio-Rad, pH 3-10)]. The extract ($10\;{\mu}g$) was rehydrated to commercially available immobilised pH gradient (IPG) strips, then the proteins were separated according to their charges as the first dimensional separation. The IPG strips were placed on Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) to separate according to molecular mass of the proteins as the second dimension. The separated protein spots were visualised by silver staining in order to compare different expression of proteins between strains. Approximately 120 spots were identified in each mini-protein electrophoresised gel, furthermore about 65 to 75 spots were regarded as identical proteins in terms of pI value and molecular weight between strains used. In addition, distinct differences were found between strains, such as 219-1, Y7 and Y14, CH150. Two representative strains were examined using strips which had pH range from 4 to 7. This strips showed a number of isoforms which were considered large spots on pH range 3-10. Furthermore, the rest of spots on pH 4-7 IPG strips appeared very distinctive compared to broad range IPG strips. 2-DE seems to be an excellent tool for analysing and identifying variations between H. pylori strains.

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Characteristics of Shear Behavior of Reinforced Concrete Beams Strengthened with Near Surface Mounted CFRP Strips (CFRP 스트립 표면매립공법으로 보강된 철근콘크리트 보의 전단거동 특성)

  • Han, Sang Hoon;Hong, Ki Nam;Shin, Byoung Gil;Lim, Jin Mook;Kwak, So Shin
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.15 no.5
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    • pp.178-189
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    • 2011
  • Tests and analyses were performed in this study to assess the shear strength of Reinforced Concrete(RC) members strengthened by the Near Surface Mounted(NSM) technique in shear, which is drawing attention as an alternative to the Carbon Fiber Reinforced Polymer(CFRP) bonding strengthening technique. Four-point bending tests were performed on 7 RC specimens without any shear reinforcement. The test variables such as the inclination of CFRP strip (45 degrees and 90 degrees), and the spacing of CFRP strip (250mm, 200mm, 150mm, 100mm) were considered. Through the testing scenarios, the effect of each test variable on the failure mode and the shear strength of the RC members strengthened by the NSM technique in shear were assessed. The test results show that the specimens with CFRP strips at 45 degrees go to failure as a result of the strip fracture, but the specimens with CFRP strips at 90 degrees go to failure as a result of the slip of strips. Strips at 45 degrees was the more effective than strips at 90 degrees, not only in terms of increasing beam shear resistance but also in assuring larger deformation capacity at beam failure. In addition, the RBSN analysis appropriately predicted the crack formation and the load-displacement response of the RC members strengthened by the NSM technique in shear.

Surface Acoustic Wave Track Changer Using Multistrip Coupler (다중스트립결합기를 이용한 탄성표면파 빔 궤적 변환기)

  • 안재영;황금찬;박용서
    • The Journal of the Acoustical Society of Korea
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    • v.3 no.2
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    • pp.23-29
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    • 1984
  • In this paper surface acoustic wave beam track changers using symmetric multistrip coupler are designed on the basis of the perturbation theory, and its characteristics are studied. SAW beam track changers, which have the configuratio of the periodic array of metal strips, are designed with the following specifications ; the number of strips : 140, the strip period: 0.25λ, the ratios of the strip width to the period a/p: 0.5, 0.375, 0.25, respectively, and approximately consistent with the theoretical values, and also the bulk wave mode and the 2nd-order effects are suppressed by these track changers.

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The Compensation of Chromaticily Coordinates on Primary Color Reaction of Urine Strips (요분석 스트립의 정색반응에 대한 색도좌표 보정)

  • 김재형;조진욱;남상희
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2001.11a
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    • pp.320-323
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    • 2001
  • A computer simulation was performed to distinguish quantitatively a color reaction in a urine analysis systems by using the spectral power distribution of LEDs, the spectral reflectance of a urine strip, and the spectral sensitivity of photodiode. The CIE tristimulus values and CIE chromaticity coordinates ware modified to be conformable with real color reactions in a urine strips. Results on color simulation showed a of real color in comparison with those obtained by Colorimeter CM2C(Color Savvy).

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Accelerated Life Testing and Validity Evaluation of Finger Strips Used for Electromagnetic Shielding Doors (전자파 차폐 도어용 핑거 스트립의 가속수명시험 및 유효성 평가)

  • Lee, Joo Hong;Kim, Do Sik;Chang, Mu Seong;Cho, Hae Yong
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.39 no.9
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    • pp.831-837
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    • 2015
  • Many persons and electronic devices are exposed to electromagnetic (EM) waves generated from magnetic resonance imaging (MRI) equipment, EM pulses (EMPs), and many other kinds of EM wave devices. Finger strips are used to provide shielding from these EM waves. Because of the high thermal conductivity of finger strips, they are used in the design of specialized doors that are installed in shielded rooms. In this study, we perform an accelerated life test using the load acceleration stress, which affects the main failure mode of finger strips. We predict the life of the finger strip under normal usage conditions based on the results of the accelerated life test. We compare the results with those predicted from the life test under normal usage conditions to evaluate the validity of accelerated life testing.

Effect of Etomidoline on the Isolated smooth Muscle of Rabbit (Etomidoline이 각종 평활근에 미치는 영향)

  • Kim, W.J.;Kim, J.H.;Sheen, Y.Y.
    • The Korean Journal of Pharmacology
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    • v.16 no.2 s.27
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    • pp.25-29
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    • 1980
  • Etomidoline ($Nonspa^{\circledR}$), which is chemically related to tertiary amine, is new synthetic antispasmodic agent with analgesic action. Antispasmodic effect of this agent is stronger than hyoscine butylbromide ($Buscopan^{\circledR}$), quaternary amine, and the absorption from intestine is also much higher. This study was undertaken to determine the effect of etomidoline on duodenal motility and other smooth muscles of rabbit. Strips of various isolated smooth muscle, 2 cm long from adult rabbits weighting about 2 kg, were suspended in a muscle chamber containing Tyrode's solution, which was bubbled with oxygen gas, and the temperature of the solution was kept constant at $38^{\circ}C$. After being washed with fresh solution several times the strips of smooth muscle attained constant motility and tonus. Etomidoline and other drugs were added in various concentrations to the chamber. Contractility of the strips was measured by using polygraph (Grass, model 7). The results are as follows: 1) In isolated rabbit atrium etomidoline produces a slight depression of contractility and the rate is also decreased. 2) On the other hand, etomidoline relaxed isolated strips of stomach, duodenal, and detrusor of rabbit. This relaxing effect of etomidoline on isolated duodenal strip of rabbit was not blocked by ${\alpha}$-adrenergic blocking agent, phenoxybenzamine, but by ${\beta}$-adrenergic blocking agent, propranolol. 3) Etomidoline did not exert any effect on isolated aorta, gall bladder, and trigone of rabbit. From the above results, it may be concluded that the relaxing effect of etomidoline on duodenal strip is related ${\beta}$-adrenergic receptor.

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A Historical Study of Beef Cooking - VII. Hoe(raw beef) - (우육(牛肉) 조리법(調理法)의 역사적(歷史的) 고찰(考察) - 1945년 이전의 문헌을 중심으로 VII.(膾) -)

  • Kim, Tae-Hong
    • Journal of the Korean Society of Food Culture
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    • v.14 no.4
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    • pp.385-393
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    • 1999
  • The purpose of this paper on Hoe (raw beef) is to investigate various kinds of its recipes, with equal focus on seasonings and sauces as well as its main and sub ingredients. The recipes of Hoe can be broadly classified into four large groups such as Salkogi Hoe(Red meat Hoe), Hankazi Naechang-yook Hoe(made from internal organs), Jap Hoe(1)(miscellaneous Meat Hoe) and Jap Hoe(II)(rolled with a whole pinenut) while the cook books written before 1945 indicate that the number of recipes reaches up to 21. The recipe of Salkogi Hoe comprises following three steps. Cut raw beef into thin strips and season them if necessary, then sprinkling sufficient pinenut powder on the strips. Its main sauce is hot pepper paste with vinegar. The recipe of Hankazi Naechang-yook Hoe uses kidney, manyplies, liver and tripes as main ingredients. Kidney should be thinly sliced with mixture of salt, seasame salt, seasame oil, and pepper as its seasonings. Regarding Manyplies, liver and tripes, there exists two possible ways to season them after cutting into strips. You can season with sesame oil and pepper or only with salt. Main ingredients of Jap Hoe(I) consist of beef, pork, kidney, manyplies, liver and tripes, among which minimum two ingredients are selected. Ingredients selected are sprinkled with pinenut powder after cutting into thin strips. And Hot pepper paste with vinegar is used as main sauce. The recipe of Jap Hoe(II) is to cut manyplies into pieces of 2Cm by 5 Cm without removing their black part and roll each piece with a whole peanut in such a manner that the peanut sticks from rolled piece.

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Ca-dependent Alteration in Basal Tone, Basal $^{45}Ca$ Uptake and $^3H-nitrendipine$ Binding in the Aorta of Spontaneously Hypertensive Rats

  • Chang, Seok-Jong;Jeon, Byeong-Hwa;Kim, Hoe-Suk
    • The Korean Journal of Physiology
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    • v.28 no.1
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    • pp.27-35
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    • 1994
  • We investigated the alterations in basal tone of aortic strips by changing the Ca concentration, basal $^{45}Ca$ uptake and $^3H-nitrendipine$ binding of the single cells of aortic smooth muscles in the spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats. While the basal tone of the aortic strips in WKY rats was not affected by alteration of Ca concentration, that in SHR was decreased by the removal of Ca from the bath solution and was recovered by the restoration of Ca to normal levels. This contraction increased in a Ca concentration-dependent manner and reached a maximum at 2 mM Ca. The basal tone of aorta in SHR was suppressed by verapamil $(10^{-6}M)$. The basal tone of aorta in SHR increased about 50% in the strips of endothelial rubbing, compared with that of intact endothelium. Basal $^{45}Ca$ uptake in the aortic single smooth muscle cells of SHR was greater than that of WKY (p<0.01), Specific bindings of $[^3H]nitrendipine$ in the aortic single smooth muscles of SHR and WKY were saturable. The dissociation constant $(K_d)\;was\;0.71{\pm}0.15\;and\;1.18{\pm}0.08nM$ SHR, respectively, and the difference in $K_d$ between two strains was statistically significant (p<0.03). The maximal binding capacity $(B_{max})\;was\;34.6{\pm}3.2\;and\;47.4{\pm}4.3\;fmol/10^6$ SHR respectively, and the difference of $(B_{max})$ between two strains was statistically significant (p<0.05). from the above results, it is suggested that the increase of Ca influx via potential-operated Ca channels and the increase of the number of dihydropyridine-sensitive Ca channels contribute to high basal tone of the aortic strips in SHR.

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Model Tests on the Reinforcement Effect of Unattached Strips to the Cantilever Retaining Wall (비정착 띠보강재의 역T형 옹벽 보강효과에 관한 모형실험)

  • Han, Gyeong-Je;Kim, Un-Yeong;Kim, Myeong-Mo
    • Geotechnical Engineering
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    • v.14 no.2
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    • pp.31-40
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    • 1998
  • To verify the reinforcing effect of the strips which are inserted in the backfill, but not connected to the face wall, model tests are executed. As the reinforcing effect is expected to reduce the active thrust acting on the retaining wall, test programmes included the measurements of the thrust. As a result. it is ascertained that the active thrusts are reduced by as much as 50%. Besides, efficient arrangement and the optimum length of the strips are verified. And the the number of reinforcing strips are increased, are close to the Rankine's hypothesis.

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