• Journal of Digital Convergence
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• v.17 no.7
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• pp.225-233
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• 2019

The purpose of this study is to investigate the affecting factors on perception of marriage needs in 20-30s unmarried workers. This study used raw data from the survey on the actual social and economic conditions of the youth of 2016. Among the 2,534 subjects, 904 unmarried workers in their 20s and 30s were included in the analysis. Dependent variables were marriage needs, and independent variables were age, sex, height, weight, education level, monthly income, parents' economic level, and health-related variables were self-rate health, stress, regular exercise, and life satisfaction included. Statistical analysis was performed binominal logistic regression analysis. In results, there were no factors influencing perception of marriage needs in 20s men, and Education was a predictor in 20s women. In 30s men, the more regular exercise, the less the monthly income, the more positive the perception of marriage needs was. In the 30s women, the better the self-rate health, the more positive the perception of marriage needs was. These findings suggest that health may affect the marriage perception of 30s unmarried workers.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.3
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• pp.544-552
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• 2019

The purpose of this study was to find concrete measures to improve the subjective health level, happiness and quality of life of the elderly according to economic level and to propose social and policy alternatives accordingly. As a research method, 63,929 elderly people aged 65 or older were surveyed using the Community Health Survey (Indicator Bank) _v09, and the frequency of health use by economic level, subjective health level, euphoria and quality of life Analysis and Chi square analysis and independent t-test. Multi variate logistic regression analysis was performed with subjective health level as a dependent variable and multiple linear regression analysis was performed to determine the factors affecting euphoria and quality of life. The results of the study are as follows. In the case of recipients, medical use was lower than that of non-recipients, lower education level, female age of 75 years or older, and less stress, In case of present or past recipients, the result of non - receipt increased as the subjective health level was worse, and the non - recipient had higher euphoria and quality of life. As a result, there is a need for alternatives to increase opportunities for medical use among the recipients, with particular attention being paid to women and elderly people over 75 years old. It is expected to be used as a basic data to effectively improve the health promotion, happiness and quality of life of the elderly people of low income group.

• Journal of the Korea Convergence Society
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• v.10 no.10
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• pp.161-171
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• 2019

This study aims the meta analysis based on the results of the empirical studies related to Job of life-long educators who are working in life-long education center, and the relationship between variables. In order to deliver this objective, articles published in domestic journals from December 2000 to April 2019 were collected, and total of 14 studies and 170 sub data were coded. With those data, the meta-analysis was executed by CMA(Comprehensive Meta Analysis) 3.0 program. The results of this meta-analysis study are as follows. First, the overall effect size associated with Job of life-long educators was 0.767, indicating between medium and large effect size. Second, the effect sizes of dependent variables that are influenced by job of life-long educators were Organization immersion, Job performance, Job satisfaction, and Job stress in order. Third, the effect size of Individual focus variables study was almost twice as big as that of mutual relation focus variables. The implications of this study were suggested based on the analysis results to provide the directivity about how we consider life-long educators related to their job.

• Journal of Applied Biological Chemistry
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• v.62 no.3
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• pp.229-237
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• 2019

More effective treatments are needed for non-alcoholic fatty liver disease (NAFLD). We hypothesized that water extracts of blackberry fruits (BF) and leaves (BL) and their combinations (BFL) reduce fat deposition in HepG2 cells and modulate shor-tchain fatty acids (SCFA) and fecal bacteria in vitro. HepG2 cells were treated with BF, BL, BFL1:2, and BFL1:3 for 1 h, and 0.5 mM palmitate was added to the cells. Moreover, low ($30{\mu}g/mL$) and high doses ($90{\mu}g/mL$) of BL and BF were applied to fecal bacteria in vitro, and SCFA was measured by GC. BL, BF, BFL1:2, and BFL1:3 reduced triglyceride deposition in the cells in a dose-dependent manner, and BFL1:2 and BFL1:3 had a stronger effect than BF. The content of malondialdehyde, an index of oxidative stress, was also reduced in BL, BF, and BFL1:2 with increasing superoxide dismutase and glutathione peroxidase activities. The mRNA expression of acetyl CoA carboxylase, fatty acid synthase, and sterol regulatory element-binding protein-1c was reduced in BL, BF, BFL1:2, and BFL1:3 compared to the control, and BFL1:2 had the strongest effect. By contrast, the carnitine palmitolytransferase-1expression, a regulator of fatty acid oxidation, increased mostly in BFL1:2 and BFL1:3. Tumor necrosis factor-${\alpha}$ and interleukin-$1{\beta}$ expression was reduced in BL compared to that in BF and BFL1:2 in HepG2 cells. Interestingly, BL increased propionate production, and BF increased butyrate and propionate production and increased total SCFA content in fecal incubation. BF increased the contents of Bifidobacteriales and Lactobacillales and decreased those of Clostridiales, whereas BL elevated the contents of Bacteroidales and decreased those of Enterobacteriales. In conclusion, BFL1:2 and BFL1:3 may be potential therapeutic candidates for NAFLD.

• Health Policy and Management
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• v.31 no.1
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• pp.74-90
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• 2021

Background: It is getting important to improve the oral health status of the elderly because oral health status may affect their health status of the whole body. In this respect, we aimed to explore the association of oral health status and behavior factors with self-rated health status by sex. Methods: Using the data from the 7th Korea National Health and Nutrition Examination Survey for health surveys and oral examinations (2016-2018), we analyzed a total of 3,070 people aged 65 or older (men: 1,329; women: 1,741). Our dependent variable, self-rated health status, was divided into two groups: not good (bad and very bad) and good (very good, good, and fair), whereas our independent variables of interest were oral health status and behavior factors. In addition to descriptive analysis and the Rao-Scott chi-square test, reflecting survey characteristics, we conducted hierarchical multivariable logistic regression analyses adjusted for socio-demographics and health status and behavior factors. All analyses were stratified by sex. Results: The proportion of people having 'not good' self-rated health was 36.5% in women but 24.5% in men. In a model adjusted for all covariates, the self-rated health status showed significant association with the self-rated oral health status. For example, in men, the risk of having 'not good' self-rated health was high in people having 'poor' (odds ratio [OR], 5.31; 95% confidence interval [CI], 2.34-12.03) self-rated oral health status and in those having 'fair' (OR, 4.03; 95% CI, 1.68-9.70) in comparison with those having 'good' self-rated oral health status. Dental status regarding speaking difficulty seemed to be very important in influencing self-rated health status. For instance, in women, compared to people having 'no discomfort' speaking difficulty, the risk of having 'not good' self-rated health was high in people having 'not bad' (OR, 1.60; 95% CI, 1.14-2.24) and 'discomfort' (OR, 1.79; 95% CI, 1.30-2.47) speaking difficulty. The covariates significantly associated with the risk of having 'not good' self-rated health were: physical activity, chronic disease, stress, and body mass index in both sexes; health insurance type and drinking only in men; and economic activity only in women. Conclusion: Oral health status and behavioral factors were associated with self-rated health status among the elderly, differently by sex. This suggests that public health policies toward better health in the elderly should take their oral health status and oral health behaviors into account in a sex-specific way.

• Journal of Mushroom
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• v.19 no.4
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• pp.310-315
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• 2021

We investigated the effect of sodium chloride-associated abiotic stress on the development of Pleurotus ostreatus. We examined the growth characteristics of fruiting bodies, constituent amino acids, and fragrance pattern to determine the effect of culturing Pleurotus ostreatus on a sawdust substrate supplemented with sodium chloride in a dose-dependent manner. Pleurotus ostreatus fruiting bodies exhibited an increasing tendency towards augmented yields when grown in the presence of 0.5% sodium chloride as compared with that grown in the control group. However, increasing the supplementation of sodium chloride from 1.0 % to 2.0% resulted in significantly decreased yields of Pleurotus ostreatus fruiting bodies in these groups as compared with that in control groups. Further assessment revealed the presence of 14 types of amino acids in the fruiting bodies, including aspartate, threonine, serine, glycine, alanine, methionine, valine, isoleucine, leucine, phenylalanine, tyrosine, lysine, histidine, and arginine, at lower levels in all the sodium chloride-treated groups than in the control group; except for glutamic acid and proline. Similarly, fragrance pattern analysis of the Pleurotus ostreatus fruiting body by chromatography confirmed that the intensity of the substances presumed to be octane compounds, to which the unique flavor of mushrooms is attributed, was lower in all the sodium chloride-treated groups than in the control group.

• Biomolecules & Therapeutics
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• v.31 no.1
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• pp.73-81
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• 2023

Sirtuins (SIRTs) belong to the nicotinamide adenine dinucleotide (NAD+)-dependent class III histone deacetylase family. They are key regulators of cellular and physiological processes, such as cell survival, senescence, differentiation, DNA damage and stress response, cellular metabolism, and aging. SIRTs also influence carcinogenesis, making them potential targets for anticancer therapeutic strategies. In this study, we investigated the anticancer properties and underlying molecular mechanisms of a novel SIRT1 inhibitor, MHY2251, in human colorectal cancer (CRC) cells. MHY2251 reduced the viability of various human CRC cell lines, especially those with wild-type TP53. MHY2251 inhibited SIRT1 activity and SIRT1/2 protein expression, while promoting p53 acetylation, which is a target of SIRT1 in HCT116 cells. MHY2251 treatment triggered apoptosis in HCT116 cells. It increased the percentage of late apoptotic cells and the sub-G1 fraction (as detected by flow cytometric analysis) and induced DNA fragmentation. In addition, MHY2251 upregulated the expression of FasL and Fas, altered the ratio of Bax/Bcl-2, downregulated the levels of pro-caspase-8, -9, and -3 proteins, and induced subsequent poly(ADP-ribose) polymerase cleavage. The induction of apoptosis by MHY2251 was related to the activation of the caspase cascade, which was significantly attenuated by pre-treatment with Z-VAD-FMK, a pan-caspase inhibitor. Furthermore, MHY2251 stimulated the phosphorylation of c-Jun N-terminal kinase (JNK), and MHY2251-triggered apoptosis was blocked by pre-treatment with SP600125, a JNK inhibitor. This finding indicated the specific involvement of JNK in MHY2251-induced apoptosis. MHY2251 shows considerable potential as a therapeutic agent for targeting human CRC via the inhibition of SIRT1 and activation of JNK/p53 pathway.

• Journal of Physiology & Pathology in Korean Medicine
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• v.36 no.6
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• pp.209-212
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• 2022

We recently reported that Gastrodia elata extracts (GEE) had an effects to protect against lipopolysaccharide-induced cognitive impairment in vivo model. In this study, we investigated the neuroprotective effects and the mechanism of action of GEE in hydrogen peroxide (H₂O₂)-induced cell death of SH-SY5Y human neuroblastoma cell. The SH-SY5Y cells were divided into five groups, including control(non-treated group), 100 μM H₂O₂, 100, 200, 500 ㎍/㎖ GEE+ 100 μM H₂O₂ groups. Pre- and co-treatment with GEE prevented cell death induced by 100 μM H₂O₂ for 24 h in SH-SY5Y cells. Our findings also showed that anti-oxidants enzymes (Cu/Zn superoxide dismutase, Mn superoxide dismutase, catalase) were up-regulated by 100 μM H₂O₂. But GEE suppressed H₂O₂-induced anti-oxidants enzymes decrease in a dose-dependent manner. Treatment with GEE also inhibited phosphorylation of eukaryotic initiation factor-2α (eIF-2α) and p38 by H₂O₂. Taken together, the neuroprotective effects of GEE in terms of recovery of antioxidant enzymes expression, down-regulation of eIF-2α and p38 phosphorylation, and inhibition of cell death are associated with reduced oxidative stress in SH-SY5Y cells.

• Journal of the Korean Geotechnical Society
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• v.23 no.6
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• pp.97-102
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• 2007

Though the stability analysis of soil slopes widely employs the limit equilibrium method, the study on the jointed rock slopes must consider the direction of joint and the characteristics of Joint at the same time. This study analyzes the result of the change in the factors which show the characteristics of discontinuity and the shape factor of rock slopes, and so on, in an attempt to validate the propriety as to the interpretation of jointed rock slope stability which uses the general finite element program. First, the difference depending on the flow rules was compared, and the factor effect study was conducted. The selected independent variables included the direction of joint which displays the mechanical characteristics of discontinuity, adhesive cohesion, friction angle, the inclination and height of rock slope which reveal the shape of slope and surcharge load. And the horizontal displacement was numerically interpreted at the 1/3 point below the slope, a dependent variable, to compare the relative degree of factor effects. The findings of study on factor effects led to the validation that the result of horizontal displacement for each factor satisfied various engineering characteristics, making it possible to be applied to stability interpretation of jointed rock slope. A modelling is possible, which considers the application of the result of real geotechnical surveys & laboratory studies and the non-linear characteristics when designing the rock slope. In addition, the stress change which may result from the natural disaster, such as precipitation, and the construction, can be expressed. Furthermore, as the complicated rock condition and the ground supporting effect can be considered through FEM, it is considered to be very useful in making an engineering decision on the cut-slope, reinforcement and so on.

• Journal of Life Science
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• v.33 no.4
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• pp.349-356
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• 2023

Gout, a chronic inflammatory arthritic disease, is characterized by hyperuricemia. Gout can be induced by an inflammatory response to monosodium urate (MSU) crystals mediated by pro-inflammatory cytokine release following activation of the NOD-like receptor protein 3 (NLRP3) inflammasome. Many sulfur-containing phytochemical compounds in garlic (Allium sativum L.) are considered active ingredients because of their potential pharmacological benefits for various diseases, but their efficacy in NLRP3 inflammasome activation-mediated gout has not been demonstrated. In this study, we investigated whether diallyl disulfide (DADS) and diallyl trisulfide (DATS), representative garlic-derived sulfur compounds, have an inhibitory effect on MSU-induced NLRP3 inflammasome activation. Our results showed that under non-cytotoxic conditions, DADS and DATS significantly blocked nitric oxide production and interleukin (IL)-1β release in response to MSU in lipopolysaccharide (LPS)-primed RAW 264.7 macrophages. DADS and DATS also attenuated enhanced expression of NLRP3 and its adapter protein, apoptosis-associated speck-like protein, which was associated with downregulation of and caspase-1 p20 and IL-1β expression, suggesting that MSU-induced LRP3 inflammasome activation was counteracted by DADS and DATS. Furthermore, DADS and DATS blocked oxidative stress, an upstream event for NLRP3 inflammasome activation, as evidenced by the fact that they scavenged reactive oxygen species (ROS) production. Taken together, our findings demonstrate that DADS and DATS suppressed NLRP3 inflammasome activation by inhibiting the ROS/NLRP3 pathway and that they have potential as treatments for NLRP3-dependent gouty arthritis.