• Journal of Plant Biotechnology
• /
• v.39 no.2
• /
• pp.106-113
• /
• 2012

Glycolysis is responsible for the conversion of glucose into pyruvate and for supplying reducing power and several metabolites. Fructose-1,6-bisphosphate aldolase (AtFBA1), a central enzyme in the glycolysis pathway, was isolated by functional complementation of the salt-sensitive phenotype of a calcineurin (CaN)-deficient yeast mutant. Under high salinity conditions, aldolase activity and the concentration of NADH were compromised. However, expression of AtFBA1 maintained aldolase activity and the NADH level in yeast cells. AtFBA1 shares a high degree of sequence identity with known class I type aldolases, and its expression was negatively regulated by stress conditions including NaCl. The fusion protein GFP-AtFBA1 was localized in the cytosol of Arabidopsis protoplasts. The seed germination and root elongation of AtFBA1 knock-out plants exhibited sensitivity to ABA and salt stress. These results indicate that AtFBA1 expression and aldolase activity is important for stress tolerance in yeast and plants.

• JSTS:Journal of Semiconductor Technology and Science
• /
• v.14 no.5
• /
• pp.632-639
• /
• 2014

Mechanical stress and the vacuum level are the two main factors dominating the quality factor of a resonator operated in the vacuum range 1 mTorr to 10 Torr. This means that if the quality factor of a resonator is very insensitive to the mechanical stress in the vacuum range, it is sensitive to mainly the ambient vacuum level. In this paper, a wafer-level packaged MEMS resonator with a highly vacuum-sensitive quality factor is presented. The proposed device is characterized by a package with out-of-plane symmetry and a suspending structure with only a single anchor. Out-of-plane symmetry helps prevent deformation of the packaged device due to thermal mismatch, and a single-clamped structure facilitates constraint-free displacement. As a result, the proposed device is very insensitive to mechanical stress and is sensitive to mainly the ambient vacuum level. The average quality factors of the devices packaged under pressures of 50, 100, and 200 mTorr were 4987, 3415, and 2127, respectively. The results demonstrated the high controllability of the quality factor by vacuum adjustment. The mechanical robustness of the quality factor was confirmed by comparing the quality factors before and after high-temperature storage. Furthermore, through more than 50 days of monitoring, the stability of the quality factor was also certified.

• Mycobiology
• /
• v.46 no.2
• /
• pp.114-121
• /
• 2018

Mon1 is a guanine nucleotide exchange factor subunit that activates the Ypt7 Rab GTPase and is essential for vacuole trafficking and autophagy in eukaryotic organisms. Here, we identified and characterized the function of Mon1, an ortholog of Saccharomyces cerevisiae Mon1, in a human fungal pathogen, Cryptococcus neoformans. Mutation in mon1 resulted in hypersensitivity to thermal stress. The mon1 deletion mutant exhibited increased sensitivity to cell wall and endoplasmic reticulum stress. However, the mon1 deletion mutant showed more resistance to the antifungal agent fluconazole. In vivo studies demonstrated that compared to the wild-type strain, the mon1 deletion mutant attenuated virulence in the Galleria mellonella insect model. Moreover, the mon1 deletion mutant was avirulent in the murine inhalation model. These results demonstrate that Mon1 plays a crucial role in stress survival and pathogenicity in C. neoformans.

• Journal of IKEEE
• /
• v.1 no.1 s.1
• /
• pp.1-10
• /
• 1997

It is well-known that rectangular bulk-Si sensors prepared by etch or epi etch-stop micromachining technology are already in practical use today, but the conventional bulk-Si sensor shows some drawbacks such as large chip size and limited applications as silicon sensor device is to be miniaturized. We consider a circular-shape SOI(Silicon-On-Insulator) micro-cavity technology to facilitate multiple sensors on very small chip, to make device easier to package than conventional sensor like pressure sensor and to provide very high over-pressure capability. This paper demonstrates the cross-functional results for stress analyses(targeting $5{\mu}m$ deflection and 100MPa stress as maximum at various applicable pressure ranges), for finding permissible diaphragm dimension by output sensitivity, and piezoresistive sensor theory from two-type SOI structures where the double SOI structure shows the most feasible deflection and small stress at various ambient pressures. Those results can be compared with the ones of circular-shape bulk-Si based sensor$^{[17]}. The SOI micro-cavity formed the sensors is promising to integrate with calibration, gain stage and controller unit plus high current/high voltage CMOS drivers onto monolithic chip.

• Computers and Concrete
• /
• v.2 no.2
• /
• pp.97-110
• /
• 2005

Rheological behaviour of fresh concrete is an important factor in controlling concrete quality. It is recognized that the measurement of the slump is not a sufficient test method to adequately characterize the rheology of fresh concrete. To further understand the slump measurement and its relationship to the rheological properties, an elasto-viscoplastic, 2-D axisymmetric finite element (FE) model is developed. The FE model employs the Bingham material model to simulate the flow of a slump test. An experimental program is carried out using the Slump Rate Machine (SLRM_II) to evaluate the finite element simulation results. The simulated slump-versus-time curves are found to be in good agreement with the measured data. A sensitivity study is performed to evaluate the effects of yield stress, plastic viscosity and cone withdrawal rate on the measured flow curve using the FE model. The results demonstrate that the computed yield stress compares well with reported experimental data. The flow behaviour is shown to be influenced by the yield stress, plastic viscosity and the cone withdrawal rate. Further, it is found that the value of the apparent plastic viscosity is different from the true viscosity, with the difference depending on the cone withdrawal rate. It is also confirmed that the value of the final slump is most influenced by the yield stress.

• Anxiety and mood
• /
• v.11 no.1
• /
• pp.26-32
• /
• 2015

Objective : The objective of this study was to examine the mental health status and its correlates of the marriage-migrant women in Seoul, Korea. Methods : One hundred and seventy marriage-migrant women and one hundred and sixteen married Korean women were recruited from community to complete Symptom Check List-90-Revision (SCL-90-R). Marriage-migrant women were also asked for their socio-demographic factors, acculturative stress, family-relationship stress, and social support. The scores on the SCL-90-R were compared between marriage-migrant women and married Korean women, and the correlates of marriage-migrant women's mental health were investigated. Results : Compared to married Korean women, marriage-migrant women showed lower levels of mental health problems including somatization, obsession-compulsion, interpersonal sensitivity, depression, anxiety, hostility, paranoid ideation, and psychoticism. Longer length of residence in Korea, lower family income, social support, higher acculturation stress, and family relationship stress were all associated with mental health problems of marriage-migrant women Results : Our results suggest that mental health of marriage-migrant women is not necessarily bad, and several factors may affect their mental health. However, further studies are required in a larger representative sample to confirm the study findings.

• Journal of the Korean Society of Manufacturing Process Engineers
• /
• v.11 no.4
• /
• pp.72-81
• /
• 2012

This study investigates structural, fatigue and modal analyses at bicycle suspension seat post. When weight is applied to the saddle, models 1 and 2 have the weakest strength at the part connected with saddle. And model 2 is greater total deformation and equivalent stress than model 1. Among the cases of nonuniform fatigue loads at models 1 and 2, 'SAE bracket history' with the severest change of load becomes most unstable but 'Sample history' becomes most stable. In case of 'Sample history' with the average stress of 0 to $-10^4MPa$ and the amplitude stress of 0 to $10^4MPa$, the possibility of maximum damage becomes 4%. This stress state can be shown with 5 to 7times more than the damage possibility of 'SAE bracket history' or 'SAE transmission'. Model 1 has better impulse relaxation and passenger sensitivity than model 2. The structural result of this study can be effectively utilized with the design of bicycle suspension seat post by investigating prevention and durability against its damage.

• Proceedings of the Korean Institute of Building Construction Conference
• /
• 2003.11a
• /
• pp.27-31
• /
• 2003

To maintain serviceability of concrete structure more than proper it is necessary not only predict service life through periodical monitor but also need monitoring system to recognize optimal time and method for repair. Recently, CPGFRP, replacing some GFRP with CF, is developed and used for monitoring concrete fraction. But dramatic resistance change of CPGFRP is showed below 0.5% strain and it is not small strain in terms of monitoring micro crack in concrete. In other word, monitoring with CF is not suitable in low stress hut hight stress. In this study, we accessed applicable possibility and reliability of CPGFRP composite as monitoring sense that is proved very sensitive to stress through domestic and oversea previous study. CPGFRP composite plays a role in specimen like steel and increases flexural strength. CPGFRP composite shows resistance increasement in micro crack. In particular, CPUFRP is more sensitive than strangage in low stress. Resistance change ratio curve is very similar to strain curve so sensitivity and reliability is very excellent to monitor concrete fracture.

• Proceedings of the Korean Institute of Building Construction Conference
• /
• 2003.05a
• /
• pp.27-31
• /
• 2003

To maintain serviceability of concrete structure more than proper it is necessary not only predict service life through periodical monitor but also need monitoring system to recognize optimal time and method for repair. Recently, CPGFRP, replacing some GFRP with CF, is developed and used for monitoring concrete fraction. But dramatic resistance change of CPGFRP is showed below 0.5％ strain and it is not small strain in terms of monitoring micro crack in concrete. In other word, monitoring with CF is not suitable in low stress but hight stress. In this study, we accessed applicable possibility and reliability of CPGFRP composite as monitoring sense that is proved very sensitive to stress through domestic and oversea previous study. CPGFRP composite plays a role in specimen like steel and increases flexural strength. CPGFRP composite shows resistance increasement in micro crack. In particular, CPGFRP is more sensitive than strangage in low stress. Resistance change ratio curve is very similar to strain curve so sensitivity and reliability is very excellent to monitor concrete fracture.

• Food Science and Preservation
• /
• v.24 no.4
• /
• pp.536-541
• /
• 2017

Hyperglycemia found in diabetes mellitus causes several physiological abnormalities including the formation of advanced glycation end products (AGEs) and oxidative stress. Accumulation of AGEs and elevation of oxidative stress plays major roles in the development of diabetic complications. Adiponectin secreted from adipocytes is known to improve insulin sensitivity and blood glucose level. Curcumin (CCM), a bioactive component of turmeric, has been reported as a potent antioxidant. Present work aimed to elucidate the roles of CCM in high glucose-induced protein glycation and intracellular events in mature adipocytes. The results demonstrated that CCM inhibited the formation of fluorescent AGEs by approximated 52% at 3 weeks of bovine serum albumin (BSA) glycation with glucose. Correspondingly, CCM decreased the levels of fructosamine and ${\alpha}-dicarbonyl$ compounds during BSA glycation with glucose. These data suggested that CCM might be a new promising anti-glycation agent. Also, CCM reduced high glucose-induced oxidative stress in a dose dependent manner, whereas CCM treatment time-dependently elevated the expression of adiponectin gene in 3T3-L1 adipocytes. The findings from this study suggested the possibility of therapeutic use of CCM for the prevention of diabetic complications and obesity-related diseases.