• Journal of Life Science
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• v.21 no.5
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• pp.664-670
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• 2011

Oxidative stress results in sustained release of heat shock protein 90 (HSP90) from vascular smooth muscle cells (VSMCs). We investigated whether extracellular HSP90 predisposed VSMCs to pro-inflammatory phenotype. Exposure of human aortic smooth muscle cells to HSP90 not only significantly enhanced CXCL10 secretion but also increased CXCL10 transcription. HSP90-mediated CXCL10 secretion was attenuated by OxPAPC, a TLR-2/4 inhibitor, and curcumin, a TLR-4 dimerization inhibitor. Inhibitors of diphenyleneiodium chloride and the Akt pathway also attenuated CXCL10 secretion in response to HSP90. The gene delivery of I${\kappa}$B using recombinant adenoviruses and treatment with resveratrol, which inhibit NF-${\kappa}$B activity, significantly attenuated HSP90-induced CXCL10 secretion from VSMCs. We propose that extracellular HSP90 contributes to an inflammatory reaction in the stressed vasculature by inducing CXCL10 expression of VSMCs, and that TLR-4, Akt, and NF-${\kappa}$B play active roles in the process.

• Korean Journal of Health Education and Promotion
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• v.25 no.3
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• pp.111-124
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• 2008

Objectives: The purpose of this study was to suggest effective methods for not only preventing college students from being exposed to smoking but also helping them stop smoking, by examining and analysing a variety of factors related to their smoking behavior. Methods: To accomplish the purpose, this study was carried out using 1,553 valid questionnaires selected from 1,584 questionnaires completed and returned by 1,800 randomly chosen students of 2 colleges and 3 universities in Gyeongsangbuk-do Province and Daegu City during the period of April 3 through April 21, 2006. Results: It was turned out that while the total smoking rate of whole students was 52.4% in male students and 9.1% in female students, the smoking rate after their admission to the college was 27.1% in males and 1.5% in females. It was also shown that both the perceived susceptibility and barrier factors of Health Belief Model were statistically significant, and that the perceived seriousness factors were entirely significant in all other variables except the body seriousness. The perceived benefit factors were significant in only the variable that there would not be any improvement in academic performance even after stopping smoking. A relation between smoking and stop smoking program or smoking prevention program showed that the non-smoking rate was significantly high among the college students who participated in such programs while in middle school. When variables having a significant effect on smoking students after their admission were analysed, it was shown that the smoking rate was higher among males than females students, and that the more the number of smoking friends is and the more the number of students thinking that stop smoking would make it difficult to release stress and make friends is, the higher the smoking rate is among students. It is required, therefore, to encourage students to continuously participate in more smoking prevention programs from middle school, and to put an emphasis on a variety of smoking-related illnesses and advantages from non-smoking. Conclusions: Since the current smoking rate among college students is relatively high but most of the smoking students surveyed are ready to actually try to stop smoking, it will be possible to significantly reduce the smoking rate among college students if proper methods are suggested to eliminate barrier factors they face while participating in the stop smoking programs.

• Journal of Music and Human Behavior
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• v.9 no.1
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• pp.37-53
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• 2012

This study is conducted to analyse the process and the pattern of motivation for musical expression in clinical music therapy for the adults in the mental health center. The participant were 8 adults purposive sampling who had more than one year music therapy experience in the mental health center and their age ranged from 27 to 53. A major category derived and analysed from the grounded theory method by Strauss and Corbin. The qualitative analysis indicated that motivation for musical expression in music therapy, which was the central phenomenon in this study, was determined by the music therapist and songs used in the sessions. The central phenomenon was caused by the therapist's friendly attitude, high musical capacity, the familarity of the selected songs and the significancy of the lyrics of selected songs. The levels of reliability about the therapist's personality and musical ability as a professional music therapist and the positive arousal of the songs used in sessions affected as the contextual condition. Spontaneous musical expression in music therapy brought the escape from reality, release of stress and sharing by the intervening condition 'empathy' and this resulted in mood regulation, the instillation of hope and group cohesion. It has been found that there were three types of motivation in music therapy: intrinsic motivation, relationship dependent extrinsic motivation and evasive motivation.

• The Korean Journal of Physiology and Pharmacology
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• v.22 no.2
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• pp.215-223
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• 2018

Intracellular $Ca^{2+}$ mobilization is closely linked with the initiation of salivary secretion in parotid acinar cells. Reactive oxygen species (ROS) are known to be related to a variety of oxidative stress-induced cellular disorders and believed to be involved in salivary impairments. In this study, we investigated the underlying mechanism of hydrogen peroxide ($H_2O_2$) on cytosolic $Ca^{2+}$ accumulation in mouse parotid acinar cells. Intracellular $Ca^{2+}$ levels were slowly elevated when $1mM\;H_2O_2$ was perfused in the presence of normal extracellular $Ca^{2+}$. In a $Ca^{2+}-free$ medium, $1mM\;H_2O_2$ still enhanced the intracellular $Ca^{2+}$ level. $Ca^{2+}$ entry tested using manganese quenching technique was not affected by perfusion of $1mM\;H_2O_2$. On the other hand, $10mM\;H_2O_2$ induced more rapid $Ca^{2+}$ accumulation and facilitated $Ca^{2+}$ entry from extracellular fluid. $Ca^{2+}$ refill into intracellular $Ca^{2+}$ store and inositol 1,4,5-trisphosphate ($1{\mu}M$)-induced $Ca^{2+}$ release from $Ca^{2+}$ store was not affected by $1mM\;H_2O_2$ in permeabilized cells. $Ca^{2+}$ efflux through plasma membrane $Ca^{2+}-ATPase$ (PMCA) was markedly blocked by $1mM\;H_2O_2$ in thapsigargin-treated intact acinar cells. Antioxidants, either catalase or dithiothreitol, completely protected $H_2O_2-induced$ $Ca^{2+}$ accumulation through PMCA inactivation. From the above results, we suggest that excessive production of $H_2O_2$ under pathological conditions may lead to cytosolic $Ca^{2+}$ accumulation and that the primary mechanism of $H_2O_2-induced$ $Ca^{2+}$ accumulation is likely to inhibit $Ca^{2+}$ efflux through PMCA rather than mobilize $Ca^{2+}$ ions from extracellular medium or intracellular stores in mouse parotid acinar cells.

• Journal of Korean Orthopaedic Sports Medicine
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• v.2 no.1
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• pp.37-43
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• 2003

Purpose: This study is to analyse the injury patterns of knee ligament and the factors influencing ligament injuries of knee, and to evaluate the changes of knee function and activity after ski injury. Materials and Methods: Thirty cases of ligament injuries of knee were studied with a questionaire, stress radiographs, magnetic resolution imaging, and physical examination. Mean age was 28.6 years old and mean follow up was 29.6 months. Clinical evaluation of Lysholm knee scoring scale was used for knee function and Tegner activity score was used for activity change after ski injury. Results: The anterior cruciate ligament (ACL) injury accompanied with medial collateral ligament(MCL) injury was most in 11 cases and isolated MCL injury was the next in 9 cases. The common types of injury mechanism were Phantom foot phenomenon (13 cases, 43$\%$) and valgus external rotation injury (12 cases, 40$\%$), which constitute 83$\%$ of all case. At the last follow up, the mean Lysholm score was 93.4 and mean Tegner activity score was 4.2. The reduced Tegner activity score after injury was 1.9. Among several injury groups, the evaluation of knee function and activity was best in the isolated MCL injury group and worst in the ACL injury accompanied with MCL injury group. The factors to influence ski injury were participation to ski class, release of binding, and skiing long time more than 2 hours. Conclusions: Lysholm score at last follow up revealed good grading, but sports activity after ski injury was reduced when compared with pre-injury state. It seems to need a active, systemic sports rehabilitation program after sports injury.

• Journal of Korean Orthopaedic Sports Medicine
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• v.2 no.1
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• pp.62-70
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• 2003

Purpose: We examined the kinematics and kinetics of the shoulder in youth baseball pitchers in light of the mechanisms of development of little league shoulder and humeral retrotorsion. Materials and Methods: The joint kinematics and the net force and torque acting on the humerus were calculated in fourteen youth pitchers throwing in a simulated game. Results: The major force component acting on the humerus was a tensile force of 378$\pm$81 N that peaked just after ball release. The predominant torque on the humerus was an external rotation torque about the long axis of the humerus. This torque reached a peak value of 35.3$\pm$6.7 Nm about 73$\%$through the pitching motion. This torque is approximately 66$\%$ of the torque required to fracture of the adult humerus. Conclusions: The direction of the humeral torque was consistent with the development of increased humeral retrotorsion in the throwing arm. Shear stress arising from the high torque during the late cocking phase likely leads to deformation the relatively weak proximal humeral epiphysis. The external rotation torque applied to the humerus during the pitch also agrees with the proposed mechanism for development little league shoulder, which has been hypothesized to be due to rotational stresses acting on the epiphysis during the throwing motion.

• Nutrition Research and Practice
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• v.6 no.2
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• pp.97-105
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• 2012

$Schizandra$ $chinensis$ Baillon is a traditional folk medicine plant that is used to treat and prevent several inflammatory diseases and cancer in Korea, but the underlying mechanisms involved in its anti-allergic activity are not fully understood. This study was designed to investigate mechanisms of anti-allergic activity of a $Schizandra$ $chinensis$ Baillon water extract (SCWE) in immunoglobulin E (IgE)-antigen complex-stimulated RBL2H3 cells and to assess whether gastric and intestinal digestion affects the anti-allergic properties of SCWE. Oxidative stress is an important consequence of the allergic inflammatory response. The antioxidant activities of SCWE increased in a concentration-dependent manner. RBL-2H3 cells were sensitized with monoclonal anti-dinitrophenol (DNP) specific IgE, treated with SCWE, and challenged with the antigen DNP-human serum albumin. SCWE inhibited ${\beta}$-hexosaminidase release and expression of interleukin (IL)-4, IL-13, and tumor necrosis factor-alpha (TNF-${\alpha}$) mRNA and protein in IgE-antigen complex-stimulated RBL2H3 cells. We found that digested SCWE fully maintained its antioxidant activity and anti-allergic activity against the IgE-antigen complex-induced activation of RBL-2H3 cells. SCWE may be useful for preventing allergic diseases, such as asthma. Thus, SCWE could be used as a natural functional ingredient for allergic diseases in the food and/or pharmaceutical industries.

• Proceedings of the SCSK Conference
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• 2003.09a
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• pp.13-34
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• 2003

Fructose-1, 6-diphosphate (FOP), a glycolytic metabolite is reported to ameliorate inflammation and inhibit the nitric oxide production in murine macrophages stimulated with endotoxin. It is also reported that FOP has cytoprotective effects against hypoxia or ischemia/reperfusion injury in brain and heart. In this study, we examined whether FDP has protective effects on UV-induced oxidative damage in skin cell culture system and human skin in vivo. FDP had a protective role in UVB-induced LDH release and ROS accumulation in HaCaT although it did not show direct radical scavenging effect in the experiment using 1, 1-diphenyl-2-picrylhydrazyl (DPPH). FDP also preserved cellular GSH content after UV irradiation in HaCaT and normal human fibroblast culture system. Cellular oxidative stress induces multiple downstream signaling pathways that regulate expression of multiple gene including MMP-1 and collagen, we examined the effects of FDP on UV-induced alteration of these protein expression in fibroblast culture and human skin in vivo. The increased MMP-1 expression in fibroblast and human skin by UV irradiation was significantly decreased by FDP. FDP also prevented the UV-induced decrease of collagen expression in fibroblast and human skin. Moreover, the decreasing the intracellular levels of reducing equivalents in human fibroblast by glutathione (GSH) depletion lowered the UVA dose threshold for reduction of procollagen expression, indicating that the differences of glutathione contents define the susceptibility of fibroblasts towards UV-induced reduction of procollagen expression. FDP also preserved cellular GSH content after UV irradiation, indicating that FDP has protective effects on UV-induced reduction of procollagen expression, which are possibly through maintaining intracellular reducing equivalent. Based on these premises, we examined the effect of daily use of a moisturizer containing FDP on facial wrinkle in comparison with vehicle moisturizer lacking FDP. In the clinical study, FDP significantly decreased facial wrinkle compared with vehicle alone after 6 months of use. Our results suggest that FDP has anti-aging effects in skin by increasing cellular antioxidant system and preventing oxidative signal and inflammatory reaction. Therefore FDP may be useful anti-aging agent for cosmetic purpose.

• The korean journal of orthodontics
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• v.25 no.2 s.49
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• pp.187-200
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• 1995

To estimate the characteristics of Korean Ni-Ti alloy orthodontic wire, this study investigated compositions, tensile properties, bending properties, heat treatment effects, and ion releasing degrees, and compared these characteristics to those of the imported Ni-Ti alloy wire. The results obtained are as follows ; 1. Ti and -Ni elements in ORTHOLLOY were in a range showing superelasticity, and there was a little difference in the Ni and Ti contents of ORTHOLLOY as compared with those of SENTALLOY. 2. The results of the tensile test concerning ORTHOLLOY exhibited a superelastic effect, indicating an area of a definite amount of stress in spite of the changes in the range from $2\%\;to\;8\%$ in the strain rate. 3. ORTHOLLOY presented higher load values than SENTALLOY in the same deflection values when the wire was tested in three-point bending. A load range displaying a superelastic effect was 80-l00g, 140-l80g, and 130-200g respectively, in wire diameters of 0.014', 0.016', and 0.018' 4. By heat treatments at $400^{\circ}C$ and at $500^{\circ}C$, a load range showing the effect of superelasticitly was lessened by the duration of the heat treatment time. The superelastic effect was destroyed as a result of the 10 minutes heat treatment at $600^{\circ}C$. 5. The quantity of the Ni ion released from ORTHOLLOY, tended to be greater than the amount of released Ni ion in SENTALLOY. The Co ion released was very little(<0.01ppm) in SENTALLOY and ORTHOLLOY irrespective of the lapse of time. Released Ni ions on the 1st day were at the maximum, and the releasing rate showed plateaus after three days. 6. The surface morphology of SENTALLOY was relatively regular irrespective of the lapse of rime, and the corrosion tendency was not observed. However, the surface morphology of ORTHOLLOY was rather irregular and shelved fitting corrosion after immersion.

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.9
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• pp.1226-1234
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• 2012

3,4,3',5'-Tetrahydroxy-trans-stilbene (piceatannol) is a derivative of resveratrol with a variety of biological activities, including anti-inflammatory, anti-proliferative, and anti-cancer activities. We assessed the mechanisms by which piceatannol inhibits inflammatory responses using lipopolysaccharide (LPS)-treated Raw264.7 murine macrophages. Piceatannol (0~10 ${\mu}mol/L$) decreased LPS-induced release of nitric oxide, tumor necrosis factor (TNF)-${\alpha}$, interleukin (IL)-6, IL-$1{\beta}$, and inhibited LPS-induced protein expression of inducible nitric oxide synthase (iNOS). Activation of nuclear factor-kappaB (NF-${\kappa}B$), activator protein (AP)-1, and signal transducer and activator of transcription 3 (STAT3) are crucial steps during an inflammatory response. Piceatannol prevented LPS-induced degradation of inhibitor of ${\kappa}B$ ($I{\kappa}B$), translocation of p65 to the nucleus, and phosphorylation of stress-activated protein kinase/c-Jun NH2-terminal kinase (SAPK/JNK). Additionally, piceatannol inhibited LPS-induced phosphorylation of STAT3 and IL-6-induced translocation of STAT3 to the nucleus. Furthermore, piceatannol increased the protein and mRNA levels of hemeoxygenase (HO)-1, the rate-limiting enzyme of heme catabolism that plays a critical role in mediating antioxidant and anti-inflammatory effects. Piceatannol further induced antioxidant response elements (ARE)-driven luciferase activity in Raw264.7 cells transfected with an ARE-luciferase reporter construct containing the enhancer 2 and minimal promoter region of HO-1. These results suggest that piceatannol exerts anti-inflammatory effects via the down-regulation of iNOS expression and up-regulation of HO-1 expression.