Nutrition Research and Practice

The Korean Nutrition Society (한국영양학회)
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Suppressive effects of $Schizandra$ $chinensis$ Baillon water extract on allergy-related cytokine generation and degranulation in IgE-antigen complex-stimulated RBL-2H3 cells

  • Chung, Mi-Ja (The Nutraceutical Bio Brain Korea 21 Project Group, Kangwon National University) ;
  • Kim, Jeong-Mi (Division of Bio-Health Technology, College of Biomedical Science, Kangwon National University) ;
  • Lee, Sang-Chul (Corporate-affiliated Research Institute of Academic-Industrial-Institutional Cooperation, Borinara Co. LTD.) ;
  • Kim, Tae-Woo (Well-Being Bioproducts RIC Center, Kangwon National University) ;
  • Kim, Dae-Jung (Well-Being Bioproducts RIC Center, Kangwon National University) ;
  • Baek, Jong-Mi (Division of Bio-Health Technology, College of Biomedical Science, Kangwon National University) ;
  • Kim, Tae-Hyuk (Division of Bio-Health Technology, College of Biomedical Science, Kangwon National University) ;
  • Lee, Jae-Sung (Division of Bio-Health Technology, College of Biomedical Science, Kangwon National University) ;
  • Kim, Kyoung-Kon (Division of Bio-Health Technology, College of Biomedical Science, Kangwon National University) ;
  • Yoon, Jin-A (Department of Food and Nutrition, Baewha Women's University) ;
  • Choe, Myeon (Division of Bio-Health Technology, College of Biomedical Science, Kangwon National University)
  • Received : 2011.07.27
  • Accepted : 2012.02.01
  • Published : 2012.04.30
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Abstract

$Schizandra$ $chinensis$ Baillon is a traditional folk medicine plant that is used to treat and prevent several inflammatory diseases and cancer in Korea, but the underlying mechanisms involved in its anti-allergic activity are not fully understood. This study was designed to investigate mechanisms of anti-allergic activity of a $Schizandra$ $chinensis$ Baillon water extract (SCWE) in immunoglobulin E (IgE)-antigen complex-stimulated RBL2H3 cells and to assess whether gastric and intestinal digestion affects the anti-allergic properties of SCWE. Oxidative stress is an important consequence of the allergic inflammatory response. The antioxidant activities of SCWE increased in a concentration-dependent manner. RBL-2H3 cells were sensitized with monoclonal anti-dinitrophenol (DNP) specific IgE, treated with SCWE, and challenged with the antigen DNP-human serum albumin. SCWE inhibited ${\beta}$-hexosaminidase release and expression of interleukin (IL)-4, IL-13, and tumor necrosis factor-alpha (TNF-${\alpha}$) mRNA and protein in IgE-antigen complex-stimulated RBL2H3 cells. We found that digested SCWE fully maintained its antioxidant activity and anti-allergic activity against the IgE-antigen complex-induced activation of RBL-2H3 cells. SCWE may be useful for preventing allergic diseases, such as asthma. Thus, SCWE could be used as a natural functional ingredient for allergic diseases in the food and/or pharmaceutical industries.

Keywords

References

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