• 한국정밀공학회지
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• 제29권3호
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• pp.307-312
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• 2012

This study aims to investigate the effect of the post weld treatments on the fatigue life of T-type welded structure made by a SM50A steel material, generally used for excavators, because changes in the geometry, material and surface properties of welded regions affect the fatigue life of welded structures. T-type test specimens were prepared by the CO2 welding of rolled steel plates (SM50A steel) with a thickness of 10 mm at a welding speed of 30 cm/min and these Ttype welded specimens were further treated by UIT (Ultrasonic Impact Treatment) and/or toegrinding post welding treatment methods. In order to investigate improvements on the fatigue life of the samples. 3-point bending fatigue tests were conducted with a stress ratio of R=0.1 under a cyclic loading environment at a frequency of 5 Hz, via a hydraulic fatigue testing machine (${\pm}100\;kN$, MTS 809). The tests were performed at room temperature. The fatigue life of UIT specimens was approximately 25 times longer than that of as-welded specimens at a stress amplitude of 281 MPa, while toe-grinding specimens exhibited 4.15 times longer fatigue life. The current results could provide important guidelines to determine the proper post weld treatment methodologies of T-type welded parts for excavators with a satisfactory fatigue life although under severe operating conditions.

• 터널과지하공간
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• 제21권3호
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• pp.164-173
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• 2011

본 연구에서는 압축공기에너지 저장 공동의 콘크리트 플러그 최적 형상을 조사하기 위해 플러그 형상에 따른 저장 공동의 안정성을 수치해석적으로 평가하였다. 고려한 플러그 형상은 원통형, 암반에 근입된 원통형, 테이퍼형, 쐐기형이었고, 강도감소법에 의한 안전율과 압축 공기의 압력으로 인해 콘크리트에 발생하는 항복 영역의 부피비를 토대로 안정성을 분석하였다. 안정성 분석 결과, 암반에 근입된 원통형과 테이퍼형 플러그가 원통형과 쐐기형 플러그보다 역학적으로 더 안정한 것으로 분석되었다. 그러나 암반에 근입된 플러그의 경우 플러그와 암반이 접촉하는 부분에서 응력 집중이 발생하여 암반에 근입된 원통형 플러그보다는 테이퍼형 플러그 가 최적의 형상인 것을 확인하였다.

• 한국자기공명학회논문지
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• 제19권3호
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• pp.99-105
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• 2015

Hsp33 is a prokaryotic molecular chaperon that exerts a holdase activity upon response to an oxidative stress at raised temperature. In particular, intramolecular disulfide bond formation between the four conserved cysteines that bind a zinc ion in reduced state is known to be critically associated with the redox sensing. Here we report the backbone NMR assignment results of the half-oxidized Hsp33, where only two of the four cysteines form an intramolecular disulfide bond. Almost all of the resolved peaks could be unambiguously assigned, although the total assignments extent reached just about 50%. Majority of the missing assignments could be attributed to a significant spectral collapse, largely due to the oxidation-induced unfolding of the C-terminal redox-switch domain. These results support two previous suggestions: conformational change in the first oxidation step is localized mainly in the C-terminal zinc-binding domain, and the half-oxidized form would be still inactive. However, some additional regions appeared to be potentially changed from the reduced state, which suggest that the half-oxidized conformation would be an intermediate state that is more labile to heat and/or further oxidation.

• Journal of Plant Biotechnology
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• 제36권4호
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• pp.407-414
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• 2009

A cDNA clone encoding phytocystatin was isolated from Brassica rapa seedlings, through rapid amplification of cDNA ends (RACE). This gene (name as Brcpi1; GenBank accession no.: EF079953) had a total length of 881 bp with an open reading frame of 609 bp, and encoded predicted polypeptide of 203 amino acid (aa) residues including a putative N-terminal signal peptide. Other relevant regions found its sequence included the G and PW conserved aa motifs, and the consensus LARFAV sequence for phytocystatins and the reactive site QVVAG. The BrCPI1 protein shared 95, 94, 81, 80 and 78% identity with other CPI proterins isolated from Brassica oleracea (BoCPI-1), Arabidopsis thaliana (AtCY SB), Glycine max (GmCPI), Oryza sativa (OsCYS-2) and Zea may (ZmCPI) at amino acid level, respectively. Southern blot analysis showed that Brcpi1 was a low copy gene. Expression pattern analysis revealed that Brcpi1 was a tissue-specific expressing gene during reproductive growth and strongly expressed at mature seedling stages. Furthermore, overexpression of Brcpi1 in transgenic Arabidopsis was enhanced tolerance to salt and cold stresses. Meanwhile the juvenile seedling of Brcpi1 transgenic plants was not affected by various concentrations ABA in MS medium. Taken together, the results showed that Brcpi1 functioned as a cysteine protease inhibitor and it exhibited a protective agent against diverse types of abiotic stress, which induced this gene in a tissue- and stress-specific manner.

• Plant Biotechnology Reports
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• 제4권1호
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• pp.85-94
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• 2010

In vitro shoot regeneration of gladiolus in three different culture systems, viz., semi-solid agar (AS), membrane raft (MR), and duroplast foam liquid (DF) cultures was evaluated following the kinetics of shoot multiplication and hyperhydricity at optimized growth regulator combinations. Compared to the AS system, matrixsupported liquid cultures enhanced shoot multiplication. The peak of shoot multiplication rate was attained at 18 days of incubation in the MR and DF systems, whereas the maximum rate in the AS system was attained at 21 days. An early decline in acceleration trend was observed in liquid cultures than the AS culture. The hyperhydric status of the regenerated shoots in the different culture systems was assessed in terms of stomatal attributes and antioxidative status. Stomatal behavior appeared to be normal in the AS and MR systems. However, structural anomaly of stomata such as large, round shaped guard cells with damage in bordering regions of stomatal pores was pronounced in the DF system along with a relatively higher $K^+$ ion concentration than in the AS and MR systems. Antioxidative status of regenerated shoots was comparable in the AS and MR systems, while a higher incidence of oxidative damages of lipid membrane as evidenced from malondialdehyde and ascorbate content was observed in the DF system. Higher oxidative stress in the DF system was also apparent by elevated activities of superoxide dismutase, ascorbate peroxidase, and catalase. Among the three culture systems, liquid culture with MR resulted in maximum shoot multiplication with little or no symptoms of hyperhydricity. Shoots in the DF system were more prone to hyperhydricity than those in the AS and MR systems. The use of matrix support such as membrane raft as an interface between liquid medium and propagating tissue could be an effective means for rapid and efficient mass propagation with little or no symptoms of hyperhydricity.

• 대기
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• 제18권2호
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• pp.137-146
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• 2008

This study examines one thermal index, perceived temperature (PT), over the Korean Peninsula during 2007 summer. Heat/cold stress has been described using air temperature and humidity for warm seasons and air temperature and wind velocity in the cold conditions, while PT is based on a heat budget model of the human body that considers air temperature, humidity, wind velocity and radiation effect regardless of climates, regions and seasons. PT is higher about $4-5^{\circ}C$ than air temperature in the summer. Humidity increases PT, while wind tends to reduces PT possibly by evaporation of water vapor. The geographical distribution of summer PT indicates that the lowest PT happened in the east central region, with the appearance of the highest PT in the inland of southern region in Korea. Although the latitudinal trend shows that PT decreases northward, inland PT is higher than that of coastal region. Compared to the heat index or the discomfort index that considers air temperature and humidity, PT represents distinctive regional characteristics of thermal comfort. The distribution of PT shows that it may be a useful thermal index for the assessment of thermal comfort or stress region in the Korean Peninsula.

• 한국지반환경공학회 논문집
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• 제13권3호
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• pp.21-27
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• 2012

동토를 대상으로 건설되는 지반구조물과 방사능 폐기물 처리장의 점토벽체의 성능을 결정하기 위해서는 정확한 세립토의 열전도계수 산정이 중요하다. 그러나 세립토의 경우 시료 성형과정에서의 교란, 포화상태 유지, 그리고 측정 장치의 장기발열로 인해 함수비 구배 발생과 같은 기존 열전도계수 측정 장비의 문제점으로 인해 열전도계수 측정에 오차가 발생한다. 본 연구에서는 압밀장비를 개조하여 열전도계수 측정용 니들프로브를 삽입한 상태에서 압밀시험을 활용하여 하중조건과 건조밀도의 변화에 따른 열전도계수를 연속적으로 측정하였다. 또한 실내실험 결과를 토대로 흙입자 구성성분의 열전도계수를 고려한 예측 모델의 신뢰성을 분석하였다.

• Perspectives in Nursing Science
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• 제12권1호
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• pp.14-22
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• 2015

Purpose: Forests have positive effects on health due to phytoncide, thus increasing physical activity and stress relief. However, research has not been conducted on the daily health benefits of existing forests. Therefore, this study attempts to compare the health status and behaviors of residents in urban and forested areas. Methods: This cross-sectional study used anthropometric measures, blood tests, heart rate variability, depression, stress, and health behavior self-reports for adults between 35 and 79 years from two regions. Results: Adults living in a forested region had better health consequences-including lower prevalence of osteoarthritis (6.4%) and mean bone mineral density (-0.84) -than those in an urban region (osteoarthritis: 13.7%; bone mineral density: -1.55). The percentage of 'physically active' participants (measured in MET-minutes) differed significantly different between the forested (49.1%) and urban (7.3%) areas. However, health behaviors such as smoking, alcohol consumption, and regular heath check-up rates were worse among residents from the forested, than the urban area. Conclusion: We concluded that more proactive forest therapy programs are needed to prove the health differences.

• 대한기계학회논문집
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• 제16권6호
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• pp.1156-1162
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• 1992

본 연구에서는 종횡비가 다른 3가지의 날카로운 모서리를 가진 타원 슬롯노즐 (AR=1,2,4)을 풍동 수축부 끝에 장착하여 타원제트의 유동특성 및 축교차현상을 3-D LDV 시스템을 이용하여 조사하였다.

• 한국식품영양과학회:학술대회논문집
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• 한국식품영양과학회 2001년도 International Symposium on Food,Nutrition and Health for 21st Century
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• pp.22-37
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• 2001

Melatonin is secreted during the hours of darkness and is thought to influence the circadian and seasonal timing of a variety of physiological processes. Serotonin N-acetyltransferase (AA-NAT) which is found to be expressed in pineal gland, retina, and various tissues, catalyses the conversion of serotonin to N-acetylserotonin and is known as the rate-limiting enzyme in the biosynthetic pathway of melatonin. The compounds that modulate the activity of AA-NAT can be used to treat serotonin-and melatonin-related diseases such as insomnia, depression and seasonal affective disorders (SAD). Several assay methods have been developed by which to measure AA-NAT activity. We have also developed a simple, rapid and sensitive AA-NAT assay method that takes advantage of differences in the organic solubilities between acetyl CoA and N-acetyltryptamine. We screened modulators of AA-NAT activity from the water extracts of the medicinal plants. We found MNP1005 which strongly inhibited the activity of AA-NAT ($IC_{50}$=2.2$\mu$M). Enzyme inhibitory kinetic studies revealed that MNP1005 exhibited a noncompetitive inhibition toward tryptamine. The antidepressant effect of MNP1005 was investigated on behavioral despair test so called forced swimming test (FST). MNP1005 significantly increased swimming behavior by reducing immobility with treatment of 10 mg/kg when compared to the vehicle-treated control group (P < 0.05). This suggests that MNP1005 possesses antidepressant activity. The influence of chronic MNP1005 treatment on the expression of brain-derived neurotrophic factor (BDNF) was examined by in situ hybridization and Northern blot. Chronic treatment of MNP1005 blocked the downregulation of BDNF mRNA in the frontal cortex and other cortex regions in response to restraint stress.