• 한국발생생물학회지:발생과생식
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• 제13권3호
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• pp.199-205
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• 2009

HSP70 has widely been induced in in vivo hyperthermia conditions in various organisms to study gene regulation and recently neuroprotectve roles of the induced gene expression under varying conditions. We investigated different responses among various tissues in zebrafish under heat shock to evaluate whether spatial and temporal expression pattern of zebrafish (z) hsp70 in transcriptional and translational level under heat shock stress in different brain regions. Heat shock groups were given for 1 h at $37^{\circ}C$ after recovery by transferring the treated animals back to $28^{\circ}C$ for 1, 2 and 24 h for recovery, respectively. Control (CTRL) group was kept at $28^{\circ}C$. At the end of treatments, five animals were collected and used for isolation of total RNAs and peptides from the corresponding tissues. Expression of zhsp70 mRNA showed different patterns in recovery periods in the tissues including the brain, eye, intestines, muscles, heart and testis by RT-PCR. Unlike the RT-PCR analysis, Northern blot analysis demonstrated nearly 30-fold increase in zhsp70 at 1 h heat shock, suggesting that RT-PCR may not be appropriate in unmasking regulation of the time-dependent zhsp70 expression. In the experiment involving different brain regions, the cerebellum showed gradual activation at 1 h to R1h and decreases in R2h and R24h, while the medulla oblongata and optic tectum showed gradual increase at R1h and decrease at R24h, indicating that different brain tissues respond specifically to heat shock in inducing zhsp70 and recovering from the heat shock status. Western blot analysis also demonstrated that the intracellular levels of zHSP70 in three different brain regions including the cerebellum, medulla oblongata and optic tectum are differently induced and recovered to normal state. These results clearly demonstrate that different regions of the body and the brain tissues are responding differently to heat shock in the aspects of its level of expression and speed of recovery.

• Ocean Systems Engineering
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• 제1권4호
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• pp.337-353
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• 2011

In the present study, an offshore platform having large partial porous cylindrical members, which are composed of permeable and impermeable cylinders, is suggested. In order to calculate the wave force on large partial porous cylindrical members, the fluid domain is divided into three regions: a single exterior region, N inner regions and N beneath regions, and the scattering wave in each fluid region is expressed by an Eigen-function expansion method. Applying Darcy's law to the porous boundary condition, the effect of porosity is simplified. Wave excitation forces and wave run up on the structures are presented for various wave conditions. For the idealized three-dimensional platform having large partial porous cylindrical members, the dynamic response evaluations of the platform due to wave forces are carried out through the modal analysis. In order to examine the effects of soil-structure interaction, the substructure method is also applied. The displacement and bending stress at the selective nodal points of the structure are computed using various input parameters, such as the shear-wave velocity of soil, the wave height and the wave period. Applying the Monte Carlo Simulation (MCS) method, the reliability evaluations at critical structure members, which contained uncertainties caused by dynamic forces and structural properties, are examined by the reliability index with the results obtained from MCS.

• Advances in environmental research
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• 제2권2호
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• pp.131-141
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• 2013

Agricultural practice and improper waste disposal in developing regions have resulted in environmental degradation in land and waters, for which low-cost, proven solutions are needed. We demonstrate in the laboratory the applications of composting and trickling filter techniques to treat olive mill wastes that can be implemented in the West Bank and other regions of the world. To a pomace waste sample from a California mill, we amended with saw dust (wood carbon source) and baking soda ($NaHCO_3$ alkalinity) at weight ratios of waste/wood/$NaHCO_3$ at 70:27:1 and composted it for periods of 11 and 48 days; the compost was used as an additive to potting soil for transplanting. The pomace sample was also blended into slurry and introduced to a water-circulating pond and trickling filter system (P/TF) to examine any inhibitive effect of the pomace on biological removal of the organic waste. The results showed the compost-amended potting soil supported plant growth without noticeable stress over 34 days and the P/TF system removed BOD and COD by >90% from the waste liquid within 2 days, with a first-order rate constant of 1.9 $d^{-1}$ in the pond. An onsite treatment design is proposed that promises implementation for agricultural waste disposal in developing regions.

• Nuclear Engineering and Technology
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• 제40권1호
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• pp.37-48
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• 2008

Flow fields inside feeder pipes have been simulated numerically using a CFD (computational fluid dynamics) code to calculate the shear stress distribution, which is the most important factor in predicting the local regions of feeder pipes highly susceptible to FAC (flow-accelerated corrosion)-induced wall thinning. The CFD approach, with schemes used in this study, to simulate the flow situations inside the CANDU feeder pipes has been verified as it showed a good agreement between the investigation results for the failed feedwater pipe at Surry unit 2 plant in the U.S. and the CFD calculation. Sensitivity studies of the three geometrical parameters, such as angle of the first and second bends, length of the first span between the grayloc hub and the first bend, and length of the second span between the first and the second bends have been performed. CFD analysis reveals that the local regions of feeder pipes of Wolsung unit 1 in Korea, on which wall thickness measurements have been performed so far, are not coincident with the worst regions predicted by the present CFD analysis located in the connection region of straight and bend pipe near the inlet part of the bend intrados. Finally, based on the results of the present CFD analysis, a guide to the selection of the weakest local positions where the measurement of wall thickness should be performed with higher priority has been provided.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 1994년도 봄 학술발표회 논문집
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• pp.1-8
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• 1994

An automated procedure which allows adaptation of spatial and time discretization simultaneously in finite element analysis of linear elastodynamic problems is presented. For dynamic problems having responses dominated by high frequency modes, such as those with impact, explosive, traveling and earthquake loads high gradient stress regions change their locations from time to time. And the time step size may need to vary in order to deal with whole process ranging from transient phase to steady state phase. As the sizes of elements in space vary in different regions, the procedure also permits different time stepping. In such a way, the best performance attainable by the finite element method can be achieved. In this study, we estimate both of the kinetic energy error and stran energy error induced by spatial and time discretization in a consistent manner. Numerical examples are used to demonstrate the performance of the procedure.

• Structural Engineering and Mechanics
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• 제46권1호
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• pp.91-110
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• 2013

This paper proposes a novel iterative procedure for the design of planar reinforced concrete structures in which the reinforcement is designed for stresses calculated in a nonlinear finite element analysis. The procedure is intended as an alternative to strut and tie modeling for the design of complex structures like deep beams with openings. Practical reinforcement arrangements are achieved by grouping the reinforcement into user defined horizontal and vertical bands. Two alternative strategies are proposed for designing the reinforcement which are designated A and B. Design constraints are specified in terms of permissible stresses and strains in the reinforcement and strains in the concrete. A case study of a deep beam with an opening is presented to illustrate the method. Comparisons are made between design strategies A and B of which B is shown to be most efficient. The resulting reinforcement weights are also shown to compare favorably with those previously reported in the literature.

• Journal of Advanced Marine Engineering and Technology
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• 제26권5호
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• pp.573-580
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• 2002

Animation and time-resolved analysis of the wake characteristics of 2-D bluff body flows were examinated by applying the multi-vision PIV to square cylinders(three angles of attack: $0^{circ}, 30^{circ} and 45^{\circ}$) and circular cylinders(three rotating speeds: 0rpm, 76rpm, 153rpm) submerged within a circulating water channel $(Re=10^4)$, The macroscopic shedding patterns and their dominant frequencies were discussed in terms of instantaneous velocity, vorticity and turbulent quantities such as turbulent intensity, turbulent kinetic energy and three Reynolds stresses. Particularly the time-averaged distribution of turbulent intensity 'islands' where their peak magnitudes were focused always small regions behind the bodies without noticeable spatial migration were particularly discovered in all cases. And the dominant frequencies of the turbulent quantities in the wake regions were two times larger than those of the velocity and vorticity.

• Computers and Concrete
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• 제16권3호
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• pp.381-397
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• 2015

Connections are the most important regions in a structural system especially for buildings in seismic zones. In R.C. structures due to large dimensions of members and lack of cognition of the stress distribution in a connection, reaching a comprehensive understanding of the connection behaviors becomes more complicated. The shear wall-to-floor slab connections in lateral load resisting systems have a potential weakness in transferring loads from slabs to shear walls which might change the path of load transformation to shear walls. This paper tries to investigate the effects of seismic load combinations on the behavior of slabs at their connection zones with the shear walls. These connection zones naturally are the most critical regions of the slabs in RC buildings. The investigation carried on in a simulated environment by considering three different structures with different shear wall layout. The final results of our study reveal that layout of shear walls in a building significantly affects the magnification of forces developed at the shear wall-floor slab connections.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 1990년도 봄 학술발표회 논문집
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• pp.6-11
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• 1990

Inelastic behavior of reinforced concrete members is very complex and affected by many factors. Therefore, though using the finite element method which is good to predict the response of R/C member, it has to be proceeded to model these factors appropriately which have influence on the behavioral characteristics of reinforced concrete members. The proposed model consists of the physical single component model having the finite size of plastic regions and the hysteretic rules, by regressing experimental data, which can idealize the hysteretic behavior of R/C member under inelastic cyclic loads. This study confirms the accuracy of the developed analytical model through comparison with the test results of R/C members having a variety of shear-to-depth ratio and maximum shear stress.

• Structural Engineering and Mechanics
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• 제58권4호
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• pp.641-659
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• 2016

This paper deals with the pure bending of incompressible elastic perfectly plastic two-layer sheets under plane strain conditions at large strains. Each layer is classified by its yield stress, shear modulus of elasticity and its initial percentage thickness in relation to the whole sheet. The solution found is semi-analytic. In particular, a numerical technique is only necessary to solve transcendental equations. The general solution is cumbersome because different analytic expressions for the radial and circumferential stresses should be adopted in different regions of the whole sheet. In particular, there are several alternative ways a plastic region (or plastic regions) can propagate. However, for any given set of material and process parameters the solution to the problem consists of a sequence of rather simple analytic expressions connected by transcendental equations. The general solution is illustrated by a simple example.