• 농업과학연구
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• 제48권1호
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• pp.163-170
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• 2021

Bacterial infections in the female reproductive tract negatively affect ovarian function, follicular development, and embryo development, leading to the eventual failure of fertilization. Moreover, bacterial lipopolysaccharides (LPS) can interfere with the immune system and reproductive system of the host animal. Therefore, this study examined the effect of LPS on the in vitro maturation (IVM) of pig oocytes. Oocytes were matured in TCM199 medium in the presence of varying concentrations of LPS (0 - 50 ㎍·mL^-1). The maturation rate, cortical granules (CGs) migration, and chromosome alignment were subsequently evaluated during the meiotic development of the oocytes. We observed a dose-dependent and significant decrease in the metaphase II (MII) rate with increasing concentrations of LPS (97.6% control [0 ㎍·mL^-1 LPS] vs. 10.4-74.9% LPS [1 - 50 ㎍·mL^-1], p < 0.05). In addition, compared to the control oocytes without LPS, higher levels of abnormal CGs distribution (18.1 - 50.0% LPS vs. 0% control), chromosome/spindle alignment (20.3 - 56.7% LPS vs. 0% control), and intracellular ROS generation were observed in oocytes matured with LPS (p < 0.05). Nitrite levels were also increased in the maturation medium derived from the oocytes matured with LPS (p < 0.05). These results indicate that LPS induces oxidative stress during IVM and affects oocyte maturation, including CGs migration and chromosome alignment of pig oocytes.

• Animal Bioscience
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• 제34권4호
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• pp.575-583
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• 2021

Objective: The aim of the study was to determine the effect of parity and the stage of lactation on the metabolic profile of cows based on the basic chemical milk components and the blood parameters. Methods: The study material consisted of high-yielding Holstein-Friesian cows. In total, 473 cows were examined. According to the parity, cows were divided into four groups: primiparous (P), and multiparous in the second (M2), in the third (M3), and in subsequent lactations (M4). The feeding of cows was based on total mixed ration (TMR) ad libitum. Milk and blood samples were collected individually from each cow three times per standard lactation period. Results: Greater exacerbation of changes in the dynamics of the blood plasma parameters examined was proved for multiparous cows. The highest value of β-hydroxybutyrate acid (0.946 mmol/L) was found for multiparous cows from group M3 at the beginning of lactation. However, it was still in the normal range. The results showed aspartate aminotransferase, and gamma-glutamyl transferase (GGT) activities in dairy cows during lactation had significant variations taking in to account stage of lactation. The highest activity of GGT was found in the group of the oldest cows and measured from 26.36 U/L at the beginning of lactation to 48.75 U/L at the end of the lactation period. Conclusion: The time-related changes in the concentrations of the biochemical parameters described differ markedly among lactating cows, though the housing conditions on the research dairy farm are highly standardised. This indicates that the ability to cope with metabolic stress is mainly affected by the individual predispositions of cows and feed nutrient supply in different stage of lactation. Especially, the feed nutrient supply (in net energy for lactation), which was the best in TMR 1 in comparison TMR 3.

• Composites Research
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• 제34권4호
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• pp.257-263
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• 2021

복합재료는 높은 비 강성 및 비 강도 특성으로 인해 기체 혹은 액체 연료를 저장하기 위한 압력 용기의 설계 및 제작에 널리 활용되고 있다. 이에 따라, 압력용기의 파열압력 또는 파단 변형률의 기계적 특성의 보다 정확한 측정은 상용화 전에 필수적 요소이다. 그러나, 기존의 시험방법을 활용한 복합재료 압력 용기의 안전성 검증은 하중 전달 매체의 변형으로 인한 추가적인 에너지 손실의 발생과, 불필요한 하중 및 모멘트의 발생 등의 한계가 있다. 따라서 본 연구에서는 수직기둥의 이론적인 하중전달 정도와 적용 가능한 수직방향 변위를 고려하여 세그먼트형 링 버스트 시험장치를 설계하였다. 또한, 세그먼트 형 링 버스트 시험장치의 균일한 압력분포를 검증하기 위해 수치해석을 활용하였고, 수압 시험방법과 링 시편의 원주방향 응력 및 변형률 분포를 비교하였다. 복합재료 압력용기의 파괴 거동을 모사하기 위해 Hashin 파손 기준을 적용하였고, 실험적으로 파단 변형률을 측정하여 이를 수치해석 결과와 비교하였다.

• The Plant Pathology Journal
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• 제37권2호
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• pp.87-98
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• 2021

To establish an infection, fungal pathogens must recognize diverse signals from host surfaces. The rice blast fungus, Magnaporthe oryzae, is one of the best models studying host-pathogen interactions. This fungus recognizes physical or chemical signals from the host surfaces and initiates the development of an infection structure called appressorium. Here, we found that protein MoAfo1(appressorium formation, MGG_10422) was involved in sensing signal molecules such as cutin monomers and long chain primary alcohols required for appressorium formation. The knockout mutant (ΔMoafo1) formed a few abnormal appressoria on the onion and rice sheath surfaces. However, it produced normal appressoria on the surface of rice leaves. MoAfo1 localized to the membranes of the cytoplasm and vacuole-like organelles in conidia and appressoria. Additionally, the ΔMoafo1 mutant showed defects in appressorium morphology, appressorium penetration, invasive growth, and pathogenicity. These multiple defects might be partially due to failure to respond properly to oxidative stress. These findings broaden our understanding of the fungal mechanisms at play in the recognition of the host surface during rice blast infection.

• Journal of Ginseng Research
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• 제46권4호
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• pp.543-549
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• 2022

Background: In physical activity or labor, the human body is in a state of high intensity stress, and all parts or physiological functions of the body respond positively to maintain or balance the need for movement. The human body has many physiological changes in the process of movement, and fatigue is the external manifestation of various complex changes inside the human body. Fatigue is also a physiological mechanism of self-protection after the body reaches a certain level of activity, which can prevent the occurrence of life-threatening excessive functional failure. The generation of fatigue is a very complex process, and its mechanism has not been concluded yet. Therefore, it is an important work to search and screen the effective components of natural plants that have anti-fatigue effect and to explore their mechanism. Methods: This was a 8-week, randomized, double-blind, placebo-controlled clinical trial. A total of 110 subjects who passed physical examination were included according to the scheme design, and randomly divided into a test group which was given KRG and a placebo control group. The calculation is carried out according to the standard of sub-high-intensity exercise test. Results: There was no adverse effect on safety index of subjects after taking red ginseng capsule. After KRG treatment, subjective strength grade is significant lower than placebo treatment. Blood lactic acid content is significantly get lower after trial in KRG group, and significant lower than placebo group. Creatine phosphokinase(CK) content is significantly get lower after trial in KRG group, and significant lower than placebo group. Conclusion: According to the criterion in the test scheme, the result shows that KRG is helpful on relieving physical fatigue.

• 항공우주시스템공학회지
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• 제16권5호
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• pp.78-85
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• 2022

산악이나 도서 지역으로의 물품수송, 재난지역의 영상정보 획득 및 긴급 구호물품 등을 수송하는데 멀티콥터 형태의 무인기를 활용하고자 하는 수요가 증가하고 있다. 이와 같은 임무를 성공적으로 수행하기 위해서는 비행 조건에 따라 발생하는 하중을 기체 구조물이 안전하게 지지하는 동시에 프롭로터의 진동 및 공탄성 안정성 확보 여부를 확인할 필요가 있다. 본 논문에서는 엔진과 발전기 조합의 하이브리드 동력 시스템이 장착된 탑재중량 40kg급 멀티콥터 무인기의 구조해석 모델 생성과 하중조건에 따른 변형 및 응력 분포 검토과정을 소개하였다. 또한 비행 속도와 기체의 피치각 조건에 따른 프롭로터 시스템의 진동 특성과 공탄성 안정성 해석 결과를 제시하였다. 프롭로터를 통해 발생하는 최대추력 및 정상, 비정상 착륙조건에 따라 기체에 작용하는 착륙하중을 검토하였으며, 구조물의 파손 없이 지지할 수 있음을 확인하였다. 기체의 비행 속도와 프롭로터의 회전속도에 따라 주요 모드별 감쇠 특성이 안정한 영역에 위치함을 확인하였다.

• Geomechanics and Engineering
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• 제27권5호
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• pp.465-479
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• 2021

One of the important causes of building and infrastructure failure, such as bridges on pile foundations, is the placement of the piles in liquefiable soil that can become unstable under seismic loads. Therefore, the overarching aim of this study is to investigate the seismic behavior of a soil-pile system in liquefiable soil using three-dimensional numerical FEM analysis, including soil-pile interaction. Effective parameters on concrete pile response, involving the pile diameter, pile length, soil type, and base acceleration, were considered in the framework of finite element non-linear dynamic analysis. The constitutive model of soil was considered as elasto-plastic kinematic-isotropic hardening. First, the finite element model was verified by comparing the variations on the pile response with the measured data from the centrifuge tests, and there was a strong agreement between the numerical and experimental results. Totally 64 non-linear time-history analyses were conducted, and the responses were investigated in terms of the lateral displacement of the pile, the effect of the base acceleration in the pile behavior, the bending moment distribution in the pile body, and the pore pressure. The numerical analysis results demonstrated that the relationship between the pile lateral displacement and the maximum base acceleration is non-linear. Furthermore, increasing the pile diameter results in an increase in the passive pressure of the soil. Also, piles with small and big diameters are subjected to yielding under bending and shear states, respectively. It is concluded that an effective stress-based ground response analysis should be conducted when there is a liquefaction condition in order to determine the maximum bending moment and shear force generated within the pile.

• Structural Engineering and Mechanics
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• 제82권2호
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• pp.173-189
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• 2022

This study presents an experimental and numerically study about the effects of fiber reinforcement ratio on the behavior of concrete-filled steel tubes (CFST) under dynamic impact loading. In literature have examined the behavior of GFRP and FRP wrapped strengthened CFST elements impact loads. However, since the direction of potential impact force isn't too exact, there is always the probability of not being matched the impact force of the area where the reinforced. Therefore, instead of the fiber textile wrapping method which strengthens only a particular area of CFST element, we used fiber-added concrete-filled elements which allow strengthening the whole element. Thus, the effect of fiber-addition in concrete on the behavior of CFST elements under impact loads was examined. To do so, six simply supported CFST beams were constructed with none fiber, 2% fiber and 10% fiber reinforcement ratio on the concrete part of the CFST beam. CFST beams were examined under two different impact loads (75 kg and 225 kg). The impactors hit the beam from a 2000 mm free fall during the experimental study. Numerical models of the specimens were created using ABAQUS finite element software and validated with experimental data. The obtained results such as; mid-span displacement, acceleration, failure modes and energies from experimental and numerical studies were compared and discussed. Furthermore, the Von Misses stress distribution of the CFST beams with different ratio of fiber reinforcements were investigated numerically. To sum up, there is an optimum amount limit of the fiber reinforcement on CFST beams. Up to this limit, the fiber reinforcement increases the structural performances of the beam, beyond that limit the fiber reinforcement decreases the performances of the CFST beam under transverse impact loadings.

• Computers and Concrete
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• 제29권 6호
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• pp.407-418
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• 2022

This paper numerically investigates the effect of changes in the mechanical properties (displacement, strain, and stress) of the ultra-high-performance concrete (UHPC) without rebar and the reinforced concrete (RC) using steel re-bars. This reinforced concrete is mostly used in the concrete bridge decks. A mixture of sand, gravel, cement, water, steel fiber, superplasticizer, and micro silica was used to fabricate UHPC specimens. The extended finite element method as used in the ABAQUS software is applied for considering the mechanical properties of UHPC, RC, and ordinary concrete specimens. To calibrate the ABAQUS, some experimental tests have been carried out in the laboratory to measure the direct tensile strength of UHPC by the compressive-to-tensile load converting (CTLC) device. This device contains a concrete specimen and is mounted on a universal tensile testing apparatus. In the experiments, three types of mixed concrete were used for UHPC specimens. The tensile strength of these specimens ranges from 9.24 to 11.4 MPa, which is relatively high compared with ordinary concrete specimens, which have a tensile strength ranging from 2 to 5 MPa. In the experimental tests, the UHPC specimen of size 150×60×190 mm with a central hole of 75 mm (in diameter)×60 mm (in thickness) was specially made in the laboratory, and its direct tensile strength was measured by the CTLC device. However, the numerical simulation results for the tensile strength and failure mechanism of the UHPC were very close to those measured experimentally. From comparing the numerical and experimental results obtained in this study, it has been concluded that UHPC can be effectively used for bridge decks.

• 한국포장학회지
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• 제28권2호
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• pp.143-149
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• 2022

PCS needs to freely switch AC and DC to connect the battery, external AC loads and renewable energy in both directions for energy efficiency. Whenever converting happens, power loss inevitably occurs. Minimization of the power loss to save electricity and convert it for usage is a very critical function in PCS. PCS plays an important role in the ESS(Energy Storage System) but the importance of stabilizing semiconductors on PCB(Printed Circuit Board) should be empathized with a risk of failure such as a fire explosion. In this study, the temperature variation inside PCS was reviewed by cooling fan on top of PCS, and the vibration characteristics of PCS were analyzed during truck transportation for reliability of the product. In most cases, a cooling fan is mounted to control the inner temperature at the upper part of the PCS and components generating the heat placed on the internal aluminum cooling plate to apply the primary cooling and the secondary cooling system with inlet fans for the external air. Results of CFD showed slightly lack of circulating capacity but simulated temperatures were durable for components. The resonance points of PCS were various due to the complexity of components. Although they were less than 40 Hz which mostly occurs breakage, it was analyzed that the vibration displacement in the resonance frequency band was very insufficient. As a result of random-vibration simulation, the lower part was analyzed as the stress-concentrated point but no breakage was shown. The steel sheet could be stable for now, but for long-term domestic transportation, structural coupling may occur due to accumulation of fatigue strength. After the test completed, output voltage of the product had lost so that extra packaging such as bubble wrap should be considered.