Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Effects of lipopolysaccharides on the maturation of pig oocytes

  • Yi, Young-Joo (Department of Agricultural Education, College of Education, Sunchon National University) ;
  • Adikari, Adikari Arachchige Dilki Indrachapa (Department of Agricultural Education, College of Education, Sunchon National University) ;
  • Moon, Seung-Tae (Department of Agricultural Education, College of Education, Sunchon National University) ;
  • Lee, Sang-Myeong (Laboratory of Veterinary Virology, College of Veterinary Medicine, Chungbuk National University) ;
  • Heo, Jung-Min (Department of Animal Science and Biotechnology, Chungnam National University)
  • Received : 2021.01.19
  • Accepted : 2021.02.09
  • Published : 2021.03.01
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Abstract

Bacterial infections in the female reproductive tract negatively affect ovarian function, follicular development, and embryo development, leading to the eventual failure of fertilization. Moreover, bacterial lipopolysaccharides (LPS) can interfere with the immune system and reproductive system of the host animal. Therefore, this study examined the effect of LPS on the in vitro maturation (IVM) of pig oocytes. Oocytes were matured in TCM199 medium in the presence of varying concentrations of LPS (0 - 50 ㎍·mL-1). The maturation rate, cortical granules (CGs) migration, and chromosome alignment were subsequently evaluated during the meiotic development of the oocytes. We observed a dose-dependent and significant decrease in the metaphase II (MII) rate with increasing concentrations of LPS (97.6% control [0 ㎍·mL-1 LPS] vs. 10.4-74.9% LPS [1 - 50 ㎍·mL-1], p < 0.05). In addition, compared to the control oocytes without LPS, higher levels of abnormal CGs distribution (18.1 - 50.0% LPS vs. 0% control), chromosome/spindle alignment (20.3 - 56.7% LPS vs. 0% control), and intracellular ROS generation were observed in oocytes matured with LPS (p < 0.05). Nitrite levels were also increased in the maturation medium derived from the oocytes matured with LPS (p < 0.05). These results indicate that LPS induces oxidative stress during IVM and affects oocyte maturation, including CGs migration and chromosome alignment of pig oocytes.

Keywords

Acknowledgement

We thank Hee-Jeong Lee for experimental assistance. This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2013R1A6A3A04063769).

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