• Science of Emotion and Sensibility
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• v.13 no.1
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• pp.129-146
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• 2010

System response times(SRTs) are getting important while increasing the function of system. In the past, most research studied SRTs on situation of computer usage. There are lacking the research on simple domain. This study focuses on behavioral and emotional effect on situation of cell phone usage(experience 1; independent variables: degree of SRTs, dependent variables: behavioral and emotional evaluation). And this study investigates the behavioral and emotional effect in same SRTs on different situation(experience 2; independent variables: degree of SRTs, usage mode, dependent variables: behavioral and emotional evaluation, stress, mental strain). The result indicated that long SRTs increased problem solving time and negative emotion. User evaluated the system differently according to usage mode after they performed the same task. In other word, if user had a strong goal, then they felt more negative emotion and mental effort than the user don't have a strong goal. In the goal mode group, it was important there are being of delay or not. This study demonstrated that SRTs and usage mode influence user's emotion and behavior performance in same task.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.10
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• pp.1471-1477
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• 2017

Objective: Since athletic performance is a most importance trait in horses, most research focused on physiological and physical studies of horse athletic abilities. In contrast, the molecular analysis as well as the regulatory pathway studies remain insufficient for evaluation and prediction of horse athletic abilities. In our previous study, we identified AXL receptor tyrosine kinase (AXL) gene which was expressed as alternative spliced isoforms in skeletal muscle during exercise. In the present study, we validated two AXL alternative splicing transcripts (named as AXLa for long form and AXLb for short form) in equine skeletal muscle to gain insight(s) into the role of each alternative transcript during exercise. Methods: We validated two isoforms of AXL transcripts in horse tissues by reverse transcriptase polymerase chain reaction (RT-PCR), and then cloned the transcripts to confirm the alternative locus and its sequences. Additionally, we examined the expression patterns of AXLa and AXLb transcripts in horse tissues by quantitative RT-PCR (qRT-PCR). Results: Both of AXLa and AXLb transcripts were expressed in horse skeletal muscle and the expression levels were significantly increased after exercise. The sequencing analysis showed that there was an alternative splicing event at exon 11 between AXLa and AXLb transcripts. 3-dimentional (3D) prediction of the alternative protein structures revealed that the structural distance of the connective region between fibronectin type 3 (FN3) and immunoglobin (Ig) domain was different between two alternative isoforms. Conclusion: It is assumed that the expression patterns of AXLa and AXLb transcripts would be involved in regulation of exercise-induced stress in horse muscle possibly through an $NF-{\kappa}B$ signaling pathway. Further study is necessary to uncover biological function(s) and significance of the alternative splicing isoforms in race horse skeletal muscle.

• Molecules and Cells
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• v.25 no.4
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• pp.559-565
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• 2008

Members of the TGA family of basic domain/leucine zipper transcription factors regulate defense genes through physical interaction with NON-EXPRESSOR OF PR1 (NPR1). Of the seven TGA family members, TGA4/octopine synthase (ocs)-element-binding factor 4 (OBF4) is the least understood. Here we present evidence for a novel function of OBF4 as a regulator of flowering. We identified CONSTANS (CO), a positive regulator of floral induction, as an OBF4-interacting protein, in a yeast two-hybrid library screen. OBF4 interacts with the B-box region of CO. The abundance of OBF4 mRNA cycles with a 24 h rhythm under both long-day (LD) and short-day (SD) conditions, with significantly higher levels during the night than during the day. Electrophoretic mobility shift assays revealed that OBF4 binds to the promoter of the FLOWERING LOCUS T (FT) gene, a direct target of CO. We also found that, like CO and FT, an OBF4:GUS construct was prominently expressed in the vascular tissues of leaf, indicating that OBF4 can regulate FT expression through the formation of a protein complex with CO. Taken together, our results suggest that OBF4 may act as a link between defense responses and flowering.

• Journal of the Korean Society for Nondestructive Testing
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• v.21 no.4
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• pp.398-405
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• 2001

The rivet joint has typical structural feature that can be initiation site for the fatigue crack due to the combination of local stress concentration around rivet hole and the moisture trapping. From a viewpoint of structural assurance, it is crucial to evaluate the size of crack around the rivet holes by appropriate nondestructive evaluation techniques. Lamb wave that is one of guided waves, offers a more efficient tool for nondestructive inspection of plates. The neural network that is considered to be the most suitable for pattern recognition has been used by researchers in NDE field to classify different types of flaws and flaw sizes. In this study, clack size evaluation around the rivet hole using the neural network based on the back-propagation algorithm has been tarried out by extracting some features from the ultrasonic Lamb wave for A12024-T3 skin panel of aircraft. Special attention was paid to reduce the coupling effect between the transducer and the specimen by extracting some features related to time md frequency component data in ultrasonic waveform. It was demonstrated clearly that features extracted from the time and frequency domain data of Lamb wave signal were very useful to determine crack size initiated from rivet hole through neural network.

• Proceedings of the Korean Biophysical Society Conference
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• 2003.06a
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• pp.39-39
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• 2003

In our previous study, WEHI-231, an immature B cell line, showed intractable increase in [C $a^{2+}$]$_{c}$ after the B-cell receptor (BCR) ligation and treatment with 2-aminoethoxydiphenylborate (2-APB), which was never observed in Bal-17, a mature B cell line (Nam et al., 2003, FEBS Lett). In this study, a whole cell voltage clamp study revealed a specific expression of a novel type of $K^{+}$ current, namely voltage-independent background-type $K^{+}$ channels (IK-bg), in WEHI-231 cells. IK-bg was dramatically increase by the application of 2-APB (50 $\square$M), which induced severe hyperpolarization of WEHI-231 from -45 ㎷ to -90 ㎷, When dialyzed with $Mg^{2+}$ and ATP-free pipette solution, a spontaneous development of IK-bg and membrane hyperpolarization were observed. IK-bg was insensitive to classical $K^{+}$ channel blockers (TEA, glibenclamide, $Ba^{2+}$(1 mM)), whereas blocked by quinine and quinidine in a voltage-dependent manner ($IC_{50}$/=6~9 $\square$M at +60㎷). Phorbol myrstate, a PKC activator, decreased the amplitude of IK-bg. Extracellular acidification (pH 6.5) slightly inhibited IK-bg. Arachidonic acid, riluzole, or hyposmotic stress could not affect the IK-bg after the full development by the intracellular dialysis with Mg-ATP-free solution. In a cell-attached mode of single channel recording from WEHI231, we found two types of voltage-independent $K^{+}$ channels with unitary conductance of 300 pS and 120 pS, respectively. Both channels showed very short mean open times and their open probabilities were increase by the application of 2-APB. In Bal-17 cells, no such $K^{+}$ current was observed in 50 cells tested. In summary, WEHI-231 immature B cells express background $K^{+}$ channels. The pharmacological properties and the large unitary conductance suggest that novel types of two-pore domain $K^{+}$ channels (2-P-K channels) might be expressed in WEHI-231, which may provide an intriguing targets of signal transduction in the immature B lymphocytes.e B lymphocytes.

• International Journal of Industrial Entomology and Biomaterials
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• v.28 no.2
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• pp.71-84
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• 2014

The full-length heat shock protein 88 (HSP88) complementary DNA (cDNA) of Paecilomyces tenuipes Jocheon-1 was obtained by screening the Paecilomyces tenuipes (P. tenuipes) Jocheon-1 Uni-Zap cDNA library and performing 5' RACE polymerase chain reaction (PCR). The P. tenuipes Jocheon-1 HSP88 cDNA contained an open reading frame (ORF) of 2,139-basepair encoding 713 amino acid residues. The deduced amino acid sequence of the P. tenuipe s Jocheon-1 HSP88 cDNA showed 77% identity to Nectria haematococca HSP88 and 45-76% identity to other fungal homologous HSP88s. Phylogenetic analysis and BLAST program analysis confirmed that the deduced amino acid sequences of the P. tenuipes Jocheon-1 HSP88 gene belonged to the ascomycetes group within the fungal clade. The P. tenuipes Jocheon-1 HSP88 also contained the conserved ATPase domain at the N-terminal region. The cDNA encoding P. tenuipes Jocheon-1 HSP88 was expressed as an 88 kilodalton (kDa) polypeptide in baculovirus-infected insect Sf9 cells. Under higher temperature conditions for the growth of the entomopathogenic fungus, mRNA expression of P. tenuipes Jocheon-1 HSP88 was quantified by real time PCR (qPCR). The results showed that heat shock stress induced a higher level of mRNA expression compared to normal growth conditions.

• Journal of the Society of Naval Architects of Korea
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• v.34 no.1
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• pp.1-10
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• 1997

Viscous flow around actual ship is calculated by an use of RANS equations. The propriety of this computing method, usefulness to hull form design and the scale effect which is the effect of viscous flow depending on the scale of ship model are investigated. Reynolds stress is modelled by using k-${\varepsilon}$ turbulence model and the law of wall is applied near the body. Body fitted coordinates are introduced for the treatment of the arbitrary 3-dimensional shape of the ship hull form. The transformed equations in the computational domain are numerically solved by an employment of FVM. In the calculation of pressure, SIMPLE method is adopted and the solution of the discretized equation is obtained by the line-by-line method with the use of TDMA The calculations of two ships, 4410 TEU container carrier and 50,000 DWT class bulk carrier, are performed at model and actual ship scale. The results are compared and discussed with the model test results which are viscous resistance, nominal wake distribution at propeller plane and limiting streamline on the hull surface. They describe the effect of stem form and the scale effect very well. In particular, the calculated nominal wake distribution and limiting streamline are agreed qualitatively with the experiments and the viscous resistance values are estimated within ${\pm}5%$ difference from the resistance tests.

• Journal of the Korean Society of Safety
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• v.18 no.2
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• pp.132-138
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• 2003

The use of long rails in high-speed railway bridges causes additional stresses due to nonlinear behaviours between the rail and bridge decks in the neighbourhood of the deck joints. In the seismic response analysis of high-speed railway bridges, since structural response is highly sensitive to properties of the ground motion, spatial variation of the ground excitation affects responses of the bridges, which in turn affect stresses in the rails. In addition, it is shown that high-speed trains need very long distances to stop when braking under seismic occurrence corresponding to operational earthquake performance level so that verification of the safe stoppage of the train is also required. In view of such additional stresses due to long rails, sensibility of structural response to the properties of the ground motion and braking distance needed by the train to stop safely, this paper proposes and establishes a time domain nonlinear dynamic analysis method that accounts for braking loads, spatial variation of the ground motion and material nonlinearities of rails to analyze long rail stresses in high-speed railway bridges subjected to seismic event. The accuracy of the proposed method is demonstrated through an application on a typical site of the Korean high-speed railway.

• Proceedings of the Korea Water Resources Association Conference
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• 2012.05a
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• pp.34-34
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• 2012

Heavy storms rainfall has caused many landslides and slope failures especially in the mountainous area of the world. Landslides and slope failures are common geologic hazards and posed serious threats and globally cause billions in monetary losses and thousands of casualies each year so that studies on slope stability and its failure mechanism under rainfall are being increasing attention of these days. Rainfall-induced slope failures are generally caused by the rise in ground water level, and increase in pore water pressures and seepage forces during periods of intense rainfall. The effective stress in the soil will be decreased due to the increased pore pressure, which thus reduces the soil shear strength, eventually resulting in slope failure. During the rainfall, a wetting front goes downward into the slope, resulting in a gradual increase of the water content and a decrease of the negative pore-water pressure. This negative pore-water pressure is referred to as matric suction when referenced to the pore air pressure that contributes to the stability of unsaturated soil slopes. Therefore, the importance is the study of saturated unsaturated soil behaviors in evaluation of slope stability under heavy rainfall condition. In an actual field, a series of failures may occur in a slope due to a rainfall event. So, this study attempts to develop a numerical model to investigate this failure mechanism. A two-dimensional seepage flow model coupled with a one-dimensional surface flow and erosion/deposition model is used for seepage analysis. It is necessary to identify either there is surface runoff produced or not in a soil slope during a rainfall event, while analyzing the seepage and stability of such slopes. Runoff produced by rainfall may result erosion/deposition process on the surface of the slope. The depth of runoff has vital role in the seepage process within the soil domain so that surface flow and erosion/deposition model computes the surface water head of the runoff produced by the rainfall, and erosion/deposition on the surface of the model slope. Pore water pressure and moisture content data obtained by the seepage flow model are then used to analyze the stability of the slope. Spencer method of slope stability analysis is incorporated into dynamic programming to locate the critical slip surface of a general slope.

• Experimental and Molecular Medicine
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• v.51 no.2
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• pp.1.1-1.14
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• 2019

Hypoxia-inducible factor-$1{\alpha}$ ($HIF-1{\alpha}$) mediates tumor cell adaptation to hypoxic conditions and is a potentially important anticancer therapeutic target. We previously developed a method for synthesizing a benzofuran-based natural product, (R)-(-)-moracin-O, and obtained a novel potent analog, MO-460 that suppresses the accumulation of $HIF-1{\alpha}$ in Hep3B cells. However, the molecular target and underlying mechanism of action of MO-460 remained unclear. In the current study, we identified heterogeneous nuclear ribonucleoprotein A2B1 (hnRNPA2B1) as a molecular target of MO-460. MO-460 inhibits the initiation of $HIF-1{\alpha}$ translation by binding to the C-terminal glycinerich domain of hnRNPA2B1 and inhibiting its subsequent binding to the 3'-untranslated region of $HIF-1{\alpha}$ mRNA. Moreover, MO-460 suppresses $HIF-1{\alpha}$ protein synthesis under hypoxic conditions and induces the accumulation of stress granules. The data provided here suggest that hnRNPA2B1 serves as a crucial molecular target in hypoxiainduced tumor survival and thus offer an avenue for the development of novel anticancer therapies.