• 한국발생생물학회지:발생과생식
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• 제24권1호
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• pp.31-41
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• 2020

We investigated the effects of endoplasmic reticulum (ER) stress inhibitor and antioxidant treatments during the micromanipulation of somatic cell nuclear transfer (SCNT) on in vitro development of SCNT embryos. Tauroursodeoxycholic acid (TUDCA), an ER stress inhibitor and vitamin C (Vit. C), an antioxidant, were treated by alone or in combination, then, the level of X-box binding protein 1 (Xbp1) splicing and the expressions of ER stress-associated genes, oxidative stress-related genes, and apoptotic genes were confirmed in the 1-cell and blastocyst stages. In the 1-cell stage, the levels of Xbp1 splicing were significantly decreased in TUDCA and Vit. C treatment groups compared to the control (p<0.05). In addition, the expression levels of most ER stress-associated genes and oxidative stress-related genes were significantly lower in all treatment groups than the control (p<0.05), and the transcript levels of apoptotic genes were also significantly lower in all treatment groups than the control (p<0.05). In the blastocyst stage, decreased expression of ER stress-, oxidative stress-, and apoptosis-related genes were observed only in some treatments. However, the blastocyst formation rates in TUDCA and Vit. C treatment groups (24.8% and 22.0%, respectively) and mean blastocyst cell number in all treatment groups (59.7±4.3 to 63.5±3.3) were significantly higher (p<0.05) than those of control. The results showed that the TUDCA or Vit. C treatment during micromanipulation inhibited both ER and oxidative stresses in the early stage of SCNT embryos, thereby reducing cell damage and promoting in vitro development.

• 한국발생생물학회지:발생과생식
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• 제23권1호
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• pp.43-54
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• 2019

We examined the effects of endoplasmic reticulum (ER) stress inhibitor treatment during the micromanipulation of porcine somatic cell nuclear transfer (SCNT) on the in vitro development of SCNT embryos. ER stress inhibitors such as salubrinal (200 nM) and tauroursodeoxycholic acid (TUDCA; $100{\mu}M$) were added to the micromanipulation medium and holding medium. The expression of X-box binding protein 1 (Xbp1), ER-stress-associated genes, and apoptotic genes in SCNT embryos was confirmed at the one-cell and blastocyst stages. Levels of Xbp1 splicing and expression of ER-stress-associated genes in SCNT embryos at the one-cell stage decreased significantly with TUDCA treatment (p<0.05). The expression of ER-stress-associated genes also decreased slightly with the addition of both salubrinal and TUDCA (Sal+TUD). The expression levels of caspase-3 and Bcl2-associated X protein (Bax) mRNA were also significantly lower in the TUDCA and Sal+TUD treatments (p<0.05). At the blastocyst stage, there were no differences in levels of Xbp1 splicing, and transcription of ER-stress-associated genes and apoptosis genes between control and treatment groups. However, the blastocyst formation rate (20.2%) and mean blastocyst cell number ($63.0{\pm}7.2$) were significantly higher (p<0.05) for embryos in the TUDCA treatment compared with those for control (12.6% and $41.7{\pm}3.1$, respectively). These results indicate that the addition of ER-stress inhibitors, especially TUDCA, during micromanipulation can inhibit cellular damage and enhance in vitro development of SCNT embryos by reducing stress levels in the ER.

• 한국토양비료학회지
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• 제34권4호
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• pp.302-309
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• 2001

수분 스트레스가 루브라오리나무(Alnus rubra) 묘목의 균근(Alpova diplophloeus) 발달과 생장에 미치는 영향을 조사하였다. 5일 주기의 수분 스트레스를 10주 동안 처리한 결과 A. diplophloeus 균근의 형성, 묘목의 생리활동 및 생장이 감소하였다. A. diplophloeus 균근균이 접종된 묘목과 접종되지 않은 묘목은 수분 스트레스에 관계없이 생리활동이나 생장에서 차이가 없었다. 질소고정활동은 탄소동화작용보다 수분스트레스에 덜 민감하였다. 이 결과는 수분스트레스 상태에서 A. diplophloeus 균근은 오리나무의 적응도(fitness)에 기여하지 못함을 의미한다.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• 제20권3호
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• pp.152-157
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• 2009

Objectives : We investigated the parenting stress and depressive mood of mothers with developmentally-delayed children and the effects of participation in a mother-child development promotion program on same. Methods : Subjects were the mothers of 20 developmentally-delayed children. The severity of the mothers' parenting stress was evaluated via the Korean version of the Parenting Stress Index, Short Form (K-PSI-SF) and the severity of their depressive symptoms were evalueted by the Korean version of the Beck Depression Inventory (K-BDI). Results : The mean K-PSI-SF score and mean K-BDI score for these mothers were 93.35 (SD=10.47) and 23.25 (SD=10.42), respectively. These scores correspond to high parenting stress and moderate depression. The mothers who participated in a mother-child attachment- promotion program showed significant decreases in their K-PSI-SF and KBDI scores. Conclusion : Our data suggest that a mother-child attachment promotion program with emphasis on child development is effective in reducing parenting stress and depressive mood in mothers of developmentally-delayed children.

• 한국지역사회생활과학회지
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• 제26권3호
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• pp.565-573
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• 2015

This study examines the relationships between stress and eating habits of adolescents based on the assumption that adolescents require large amounts of nutrients and that eating habits affect their physical development, academic achievement, and emotional development. For this purpose, 213 adolescents in Gangwondo, Korea, were surveyed. Many studies have reported that stress causes changes in eating habits and affects health status. Based on the dietary action guide scores according to stress categories, eating instant foods less was significant (p<0.001), eating fast foods less was significant (p<0.05), and have soft drinks less was significant (p<0.001). The results suggest the importance of balanced nutrition based on the formation of right dietary habits for adolescents. Good nutrition can be determined through right knowledge of nutrition and dietary habits, and right dietary habits can improve health and physical development as well as mental and emotional stability. The analysis did not compare other regions, and therefore any generalization of the results should be made with caution. However, the results offer important insights into stress among adolescents for their effective counseling.

• Nuclear Engineering and Technology
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• 제54권12호
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• pp.4481-4490
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• 2022

In this study, a new rupture disk corrosion test (RDCT) method was developed for real-time detection of stress corrosion cracking (SCC) initiation of Alloy 600 in a primary water environment of pressurized water reactors. In the RDCT method, one side of a disk specimen was exposed to a simulated primary water at high temperature and pressure while the other side was maintained at ambient pressure, inducing a dome-shaped deformation and tensile stress on the specimen. When SCC occurs in the primary water environment, it leads to the specimen rupture or water leakage through the specimen, which can be detected in real-time using a pressure gauge. The tensile stress applied to the disk specimen was calculated using a finite element analysis. The tensile stress was calculated to increase as the specimen thickness decreased. The SCC initiation time of the specimen was evaluated by the RDCT method, from which result it was found that the crack initiation time decreased with the decrease of specimen thickness owing to the increase of applied stress. After the SCC initiation test, many cracks were observed on the specimen surface in an intergranular fracture mode, which is a typical characteristic of SCC in the primary water environment.

• 한국고분자학회:학술대회논문집
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• 한국고분자학회 2006년도 IUPAC International Symposium on Advanced Polymers for Emerging Technologies
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• pp.361-361
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• 2006

To clarify the structure development of cyclic olefin copolymers in stretching and relaxation processes, on-line measurements of optical retardation and tensile force were performed. Birefringence increased continuously whereas stress showed yielding at stretching temperatures of $160^{\circ}C$. At $170^{\circ}C$, stress yielding was not observed and stress and birefringence increased monotonously with an increase in the strain. In the relaxation process, stress and birefringence decreased monotonously at all the temperatures examined. The slope for the stress vs. birefringence relation at initial stage of stretching increased with an increase in temperature, whereas that for the relaxation stage was significantly larger that for the stretching process, and therefore showed a significant hysteresis.

• 한국항해학회지
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• 제25권3호
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• pp.225-236
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• 2001

In the recent years, major ship registers have demanded improved safety on the hull stress of large bulk carriers which are on navigation or cargo handling in harbour. Under these circumstances, a system that monitors hull stress and ship condition is being more and more important. If efficient and appropriate navigational information is given, safety of navigation would be greatly improved. The major ship registers of the globe are investing a great effort on the development of a system that monitors the hull stress of ship. Using this system, information of hull stress and ship motion is given to the users and also the data is stored on the external data storage system simultaneously. Through this study, a software that monitors hull stress was developed. Not only can randomized input-data of the standard hardwares be applied to the system, but also this system can be operated on and applied to real hardware systems.

• Journal of Plant Biotechnology
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• 제37권4호
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• pp.562-566
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• 2010

SOD2-transgenic $T_3$ petunia line (A2-36-2-1-1-35) was treated with different levels of methyl viologen (MV) to determine its resistance to oxidative stress. Four (4) levels of MV (0, 100, 200, and $400\;{\mu}M$) were applied. The SOD2-transgenic $T_3$ petunia line exhibited a very significant oxidative stress resistance at the highest MV concentration ($400\;{\mu}M$) treatment compared to non-transgenic plant. RNA and protein expression of SOD2 transgene and higher parenchyma cell density in the transgenic petunias exhibiting resistance to oxidative stress proves its contribution to the expression of its resistance to oxidative stress.

• Journal of Ginseng Research
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• 제37권4호
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• pp.442-450
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• 2013

The aim of this study was to determine and compare the preventive effect of Korean White Ginseng and Red Ginseng on oxidative stress in $H_2O_2$-treated HepG2 cells. The roots of ginseng were extracted with 70% methanol and partitioned with butanol to obtain saponin fractions, which have been known as bioactive constituents of ginseng. 2',7'-Dichlorofluorescein diacetate (DCF-DA) assay and malondialdehyde (MDA) content were measured for evaluating intracellular reactive oxygen species (ROS) generation. Also, mRNA expressions and activities of antioxidant enzymes were analyzed to determine the antioxidant activity of saponin or non-saponin fractions of ginsengs. According to DCF-DA assay, $H_2O_2$-induced MDA release and ROS generation were significantly reduced by treatment with saponin fractions of white and red ginseng roots. Also, saponin fractions increased effectively intracellular antioxidant enzyme activities including catalase, glutathione peroxidase and superoxide dismutase in $H_2O_2$-treated HepG2 hepatoma cells. In general, red ginseng was more effective than white ginseng for reducing oxidative stress. These results indicate that administration of red ginseng may certainly contribute relatively stronger than white ginseng to prevent from damaging liver function by oxidative stress.