• The Plant Pathology Journal
• /
• v.38 no.4
• /
• pp.372-382
• /
• 2022

Soybean is an important source of protein and for a wide range of agricultural, food, and industrial applications. Soybean is being affected by Xanthomonas citri pv. glycines, a causal pathogen of bacterial pustule disease, result in a reduction in yield and quality. Diverse microbial communities of plants are involved in various plant stresses is known. Therefore, we designed to investigate the microbial community differentiation depending on the infection of X. citri pv. glycines. The microbial community's abundance, diversity, and similarity showed a difference between infected and non-infected soybean. Microbiota community analysis, excluding X. citri pv. glycines, revealed that Pseudomonas spp. would increase the population of the infected soybean. Results of DESeq analyses suggested that energy metabolism, secondary metabolite, and TCA cycle metabolism were actively diverse in the non-infected soybeans. Additionally, Streptomyces bacillaris S8, an endophyte microbiota member, was nominated as a key microbe in the healthy soybeans. Genome analysis of S. bacillaris S8 presented that salinomycin may be the critical antibacterial metabolite. Our findings on the composition of soybean microbiota communities and the key strain information will contribute to developing biological control strategies against X. citri pv. glycines.

• Nuclear Engineering and Technology
• /
• v.55 no.8
• /
• pp.2823-2834
• /
• 2023

Austenitic stainless steel welds (ASSWs) of nuclear components undergo aging-related degradations caused by high temperature and neutron radiation. Since irradiation leads to the change of material characteristics, relevant quantification is important for long-term operation, but limitations exist. Although ion irradiation is utilized to emulate neutron irradiation, its penetration depth is too shallow to measure bulk properties. In this study, a systematic approach was suggested to estimate mechanical properties of ion irradiated 308 ASSW. First of all, weld specimens were irradiated by 2 MeV proton to 1 and 10 dpa. Microstructure evolutions due to irradiation in δ-ferrite and austenite phases were characterized and micropillar compression tests were performed. In succession, dislocation density based stress-strain (S-S) relationships and quantification models of irradiation defects were adopted to define phases in finite element analyses. Resultant microscopic S-S curves were compared to verify material parameters. Finally, macroscopic behaviors were calculated by multiscale simulations using real microstructure based representative volume element (RVE). Validity of the approach was verified for the unirradiated specimens such that the estimated S-S curves and 0.2% offset yield strengths (YSs) which was 363.14 MPa were in 10% agreement with test. For irradiated specimens, the estimated YS were 917.41 MPa in 9% agreement.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.25 no.1
• /
• pp.61-69
• /
• 2001

EFP(Fitness For Purpose) type defect assessment methodologies based on ECA(Engineering Critical Analysis) have been established and are in use for the structural integrity evaluation of gas pipelines. ECA usually includes the fracture mechanics analysis, and it is assumed that the J-integral uniquely characterizes the crack-tip stress-strain field. However, it has been proven that the J-integral alone can not be sufficient to characterize the crack-tip field under low levels of constraint with a single parameter. Since pipeline structures are made of ductile material, locally loaded in tension, cracks may experience low level of constraint, and therefore, J-dominance will be lost. For this reason, the level of constraint must be quantified to establish a precise assessment procedure for pipeline defects. The objective of this paper is to investigate the fracture behavior of a crack in gas pipeline(KS D 3507) by quantifying the level of constraint. For this purpose, tensile tests and CTOD tests were performed at room temperature(24$\^{C}$) and low temperature(-40$\^{C}$) to obtain the material properties. J-Q analyses were performed for SENB and SENT specimens based on 2-D finite element analyses, in order to investigate the in-plane constraint effects on pipeline defects. For precise assessment of cracks, especially shallow cracks, in KS D 3507 pipeline, constraint effect must be considered.

• Steel and Composite Structures
• /
• v.28 no.5
• /
• pp.641-653
• /
• 2018

This paper presents experiment and bearing capacity analyses of steel dual-lintel column (SDC) joints in Chinese traditional style buildings. Two SDC interior joints and two SDC exterior joints, which consisted of dual box-section lintels, circular column and square column, were designed and tested under low cyclic loading. The force transferring mechanisms at the panel zone of SDC joints were proposed. And also, the load-strain curves at the panel zone, failure modes, hysteretic loops and skeleton curves of the joints were analyzed. It is shown that the typical failure modes of the joints are shear buckling at bottom panel zone, bending failure at middle panel zone, welds fracturing at the panel zone, and tension failure of base metal in the heat-affected zone of the joints. The ultimate bearing capacity of SDC joints appears to decrease with the increment of axial compression ratio. However, the bearing capacities of exterior joints are lower than those of interior joints at the same axial compression ratio. In order to predict the formulas of the bending capacity at the middle panel zone and the shear capacity at the bottom panel zone, the calculation model and the stress state of the element at the panel zone of SDC joints were studied. As the calculated values showed good agreements with the test results, the proposed formulas can be reliably applied to the analysis and design of SDC joints in Chinese traditional style buildings.

• The Journal of Engineering Geology
• /
• v.30 no.1
• /
• pp.1-15
• /
• 2020

The Mohr-Coulomb model is mainly used in evaluating the behavior of the ground in numerical analyses of domestic ground excavation. This study analyzes its limitations and compares its numerical results with the hyperbolic model, a model that closely follows actual ground behavior during excavation. Recent applications of the Mohr-Coulomb model in Korea have tended to impose arbitrary special boundary conditions to control the problem of excessive heaving of the ground excavation surface. This adjustment only controls the size of the heaving of the excavation surface, implying that the ground behavior is distorted from the actual behavior. This study compares results from the hyperbolic model (hardening soil model) and the Mohr-Coulomb model, and confirms that the hyperbolic model provides both a more-suitable solution to the problem of heaving during excavation and the actual stress-strain behavior. In numerical analyses of ground excavation, the hyperbolic model is expected to give results consistent with the actual ground behavior.

• Steel and Composite Structures
• /
• v.46 no.4
• /
• pp.451-469
• /
• 2023

The use of precast hollow-core slabs (PCHCS) in civil construction has been increasing due to the speed of execution and reduction in the weight of flooring systems. However, in the literature there are no studies that present a finite element model (FEM) to predict the load-slip relationship behavior of pushout tests, considering headed stud shear connector and PCHCS placed at the upper flange of the downstand steel profile. Thus, the present paper aims to develop a FEM, which is based on tests to fill this gap. For this task, geometrical non-linear analyses are carried out in the ABAQUS software. The FEM is calibrated by sensitivity analyses, considering different types of analysis, the friction coefficient at the steel-concrete interface, as well as the constitutive model of the headed stud shear connector. Subsequently, a parametric study is performed to assess the influence of the number of connector lines, type of filling and height of the PCHCS. The results are compared with analytical models that predict the headed stud resistance. In total, 158 finite element models are processed. It was concluded that the dynamic implicit analysis (quasi-static) showed better convergence of the equilibrium trajectory when compared to the static analysis, such as arc-length method. The friction coefficient value of 0.5 was indicated to predict the load-slip relationship behavior of all models investigated. The headed stud shear connector rupture was verified for the constitutive model capable of representing the fracture in the stress-strain relationship. Regarding the number of connector lines, there was an average increase of 108% in the resistance of the structure for models with two lines of connectors compared to the use of only one. The type of filling of the hollow core slab that presented the best results was the partial filling. Finally, the greater the height of the PCHCS, the greater the resistance of the headed stud.

• Computers and Concrete
• /
• v.31 no.5
• /
• pp.419-432
• /
• 2023

Beam-column joints are a critical component of reinforced concrete frame structures. They are responsible for transferring forces between adjoining beams and columns while limiting story drifts and maintaining structural integrity. During severe loading, beam-column joints deform significantly, affecting, and sometimes governing, the overall response of frame structures. While most failure modes for beam and column elements are commonly considered in plastic-hinge-based global frame analyses, the beam-column joint failure modes, such as concrete shear and reinforcement bond slip, are frequently omitted. One reason for this is the dearth of published guidance on what type of hinges to use, how to derive the joint hinge properties, and where to place these hinges. Many beam-column joint models are available in literature but their adoption by practicing structural engineers has been limited due to their complex nature and lack of practical application tools. The objective of this study is to provide a comparative review of the available beam-column joint models and present a practical joint modeling approach for integration into commonly used global frame analysis software. The presented modeling approach uses rotational spring models and is capable of modeling both interior and exterior joints with or without transverse reinforcement. A spreadsheet tool is also developed to execute the mathematical calculations and derive the shear stress-strain and moment-rotation curves ready for inputting into the global frame analysis. The application of the approach is presented by modeling a beam column joint specimen which was tested experimentally. Important modeling considerations are also presented to assist practitioners in properly modeling beam-column joints in frame analyses.

• Structural Engineering and Mechanics
• /
• v.84 no.4
• /
• pp.437-452
• /
• 2022

For the first time, the higher-order shear and normal deformable plate theory (HOSNDPT) is used for the vibration and flutter analyses of the multilayer functionally graded graphene platelets reinforced composite (FG-GPLRC) plates under supersonic airflow. For modeling the supersonic airflow, the linear piston theory is adopted. In HOSNDPT, Legendre polynomials are used to approximate the components of the displacement field in the thickness direction. So, all stress and strain components are encountered. Either uniform or three kinds of non-uniform distribution of graphene platelets (GPLs) into polymer matrix are considered. The Young modulus of the FG-GPLRC plate is estimated by the modified Halpin-Tsai model, while the Poisson ratio and mass density are determined by the rule of mixtures. The Hamilton's principle is used to obtain the governing equations of motion and the associated boundary conditions of the plate. For solving the plate's equations of motion, the Galerkin approach is applied. A comparison for the natural frequencies obtained based on the present investigation and those of three-dimensional elasticity theory shows a very good agreement. The flutter boundaries for FG-GPLRC plates based on HOSNDPT are described and the effects of GPL distribution patterns, the geometrical parameters and the weight fraction of GPLs on the flutter frequencies and flutter aerodynamic pressure of the plate are studied in detail. The obtained results show that by increasing 0.5% of GPLs into polymer matrix, the flutter aerodynamic pressure increases approximately 117%, 145%, 166% and 196% for FG-O, FG-A, UD and FG-X distribution patterns, respectively.

• Journal of Microbiology and Biotechnology
• /
• v.34 no.4
• /
• pp.871-879
• /
• 2024

Our group had isolated Bifidobacterium breve strain BS2-PB3 from human breast milk. In this study, we sequenced the whole genome of B. breve BS2-PB3, and with a focus on its safety profile, various probiotic characteristics (presence of antibiotic resistance genes, virulence factors, and mobile elements) were then determined through bioinformatic analyses. The antibiotic resistance profile of B. breve BS2-PB3 was also evaluated. The whole genome of B. breve BS2-PB3 consisted of 2,268,931 base pairs with a G-C content of 58.89% and 2,108 coding regions. The average nucleotide identity and whole-genome phylogenetic analyses supported the classification of B. breve BS2-PB3. According to our in silico assessment, B. breve BS2-PB3 possesses antioxidant and immunomodulation properties in addition to various genes related to the probiotic properties of heat, cold, and acid stress, bile tolerance, and adhesion. Antibiotic susceptibility was evaluated using the Kirby-Bauer disk-diffusion test, in which the minimum inhibitory concentrations for selected antibiotics were subsequently tested using the Epsilometer test. B. breve BS2-PB3 only exhibited selected resistance phenotypes, i.e., to mupirocin (minimum inhibitory concentration/MIC >1,024 ㎍/ml), sulfamethoxazole (MIC>1,024 ㎍/ml), and oxacillin (MIC >3 ㎍/ml). The resistance genes against those antibiotics, i.e., ileS, mupB, sul4, mecC and ramA, were detected within its genome as well. While no virulence factor was detected, four insertion sequences were identified within the genome but were located away from the identified antibiotic resistance genes. In conclusion, B. breve BS2-PB3 demonstrated a sufficient safety profile, making it a promising candidate for further development as a potential functional food.

• Journal of Korean Tunnelling and Underground Space Association
• /
• v.16 no.1
• /
• pp.25-50
• /
• 2014

In order to identify a load transfer mechanism of ground anchors, the behavior of multi load transfer ground anchor systems was investigated and compared with those of compression type anchors and tension type anchors. Large scale model tests were performed and stress-strain relationships were obtained. The load transfer mechanism of ground anchors was also investigated in the field tests. Finally, numerical analyses to predict the load-displacement relationships of anchors were conducted. It is concluded that the load transfer characteristics of MLT anchors are mechanically much more superior in the pull-out resistance effect than those of existing compression and tension type anchors. From the results of research work, we could suggest that the max pull-out capacity of anchor capacity to each the soil condition. Also, the MLT anchors can be used to achieve both structural enhancement and economic construction in earth retaining or supporting structures.