• Title/Summary/Keyword: streptozotocin-induced hyperglycemic rats

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The Effects of Saengjingamrotang and Saengjingamrotang plus Radix Trichosanthis in Streptozotocin induced Hyperglycemic Rats (생진감로탕(生津甘露湯)과 생진감로탕가천화분(生津甘露湯加天花粉)이 고혈당(高血糖) 백서(白鼠)에 미치는 영향(影響))

  • Baek Jung-Han;Kang Seok-Bong
    • Journal of Society of Preventive Korean Medicine
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    • v.3 no.1
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    • pp.83-100
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    • 1999
  • This present study was carried out to investigate the effect of Saengjingamrotang and Saengjingamrotang plus Radix Trichosanthis in streptozotocin induced hyperglycemic rats. Body weight, serum levels of glucose insulin total cholesterol triglyceride and urine levels of volume glucose protein were measured in streptozotocin induced hyperglycemic rats orally receiving extracts of Saengjingamrotang and Saengjingamrotang plus Radix Trichosanthis for 4 days. According to above mentioned results, Saengjingamrotang and Saengjingamrotang plus Radix Trichosanthis have the effect of decreased blood sugar, serum lipid levels and urine volume, protein, glucose in streptozotocin induced hyperglycemic rats, and so they were expected to be appled to the treatment of diabetes mellitus.

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The Effect of Chonhwasan and Okchunsan in Streptozotocin induced Hyperglycemic Rats (천화산(天花散)과 옥천산(玉泉散)이 고혈당(高血糖) 백서(白鼠)에 미치는 영향(影響))

  • Kim Gweon-Cheol;Park Ji-Hyeon;Kang Seog-Bong
    • Journal of Society of Preventive Korean Medicine
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    • v.4 no.1
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    • pp.132-143
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    • 2000
  • This present study was carried out to investigate the effect of Chonhwasan and Okchunsan in streptozotocin induced hyperglycemic rats. Body weight, serum levels of glucose, insulin, total cholesterol triglyceride and urine levels of volume, glucose, protein were measured in streptozotocin induced hyperglycemic rats orally receiving extracts of Chonhwasan and Okchunsan plus Radix Trichosandis for 4 days. According to the result, Chonhwasan and Okchunsan have the effect of decreased body weight delate and decreased blood sugar, serum lipid metabolism and urine volume, glucose, protein in streptozotocine induced hyperglycemic rats, and they were expected to be applied to the treatment of Diabetes Mellitus.

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The Physiological Activities of Kyung Ok-Ko(II) -Effects on the Hyperglycemia, Hypertension, Anti-Fatigue and Decrease of Body Weight- (경옥고의 생리활성 (II) -고혈당, 고혈압, 지구력 및 체중감소에 미치는 영향-)

  • Whang, Wan-Kyunn;Oh, In-Se;Lee, Suk-Hee;Choi, Soo-Bu;Kim, Il-Hyuk
    • Korean Journal of Pharmacognosy
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    • v.25 no.1
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    • pp.51-58
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    • 1994
  • The studies were conducted to investigate the anti-diabetic activities on the hyperglycemia induced by streptozotocin in rats, anti-hypertensive activities in SHR, anti-fatigue and decrease of body weight activities in mice by Kyung Ok-Ko water extract and drink, which is a traditional preparation in Korea. 1. The blood glucose levels of streptozotocin-induced hyperglycemic rats were dose-dependently decreased by administrations of various doses(300, 600, 1200 mg/kg) of Kyung Ok-Ko water extract. Particularly, administration of 600, 1200 mg/kg were significantly shown to decrease glucose levels comparing with control group. 2. The serum total cholesterol levels of streptozotocin-induced hyperglycemic rats were dose-dependently decreased by administrations of various doses(300, 600, 1200 mg/kg) of Kyung Ok-Ko water extract. Particularly, administration of 600, 1200 mg/kg were significantly shown to decrease total cholesterol levels comparing with control group. 3. The serum triglyceride levels of streptozotocin-induced hyperglycemic rats were dose-dependently decreased by administrations of various doses(300, 600, 1200 mg/kg) of Kyung Ok-Ko water extract. Particularly, administration of 600, 1200 mg/kg were significantly shown to decrease triglyceride levels comparing with control group. 4. The blood pressure in SHRs were dose-dependently lower descended by administration of Kyung Ok-Ko water extract 300, 600, and 1200 mg/kg, respectively. 5. The swimming time of Kyung Ok-Ko water extract(100, 200, 400 mg/kg) and drink(0.7, 1.4, 2.1 ml/kg) were significantly extended to all experimental group dose-dependantly. 6. The decrease of body weight of Kyung Ok-Ko water extract(100, 200, 400 mg/kg) and drink(0.7, 1.4, 2.1 ml/kg) were significantly evaluated dose-dependently in all experimental group.

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Effects of Indongdeung Jigolpi-tang applied to Soyangin on Hyperglycemic Rats induced by Streptozotocin (소양인(少陽人)에 응용(應用)되는 인동등지골피탕(認冬藤地骨皮湯)이 Streptozotocin 투여(投與) 고혈당(高血糖) 백서(白鼠)에 미치는 영향(影響))

  • Ko, Ki Duk;Hong, Sun-Yong
    • Journal of Sasang Constitutional Medicine
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    • v.2 no.1
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    • pp.149-166
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    • 1990
  • In order to investigate experimented effects of Indongdeung Jigolpi-Tang, which is applied to Soyangin, to diabetes, the measurement, comparison and observation made on the contents of volumes of blood glucose, total cholesterol, triglyceride, phospholipid, total protein and electrolyte($K^+$, $Na^+$) in blood serum by giving a high blood glucose, induced by streptozotocin, to hyperglycemic rats, dosing the cooked liquid of I.J.T., and following conclusion was attained. 1. The volume of glucose urine, induced by streptozotocin to hyperglycemic rats, was shown an increase in comparision to a normal flock when $12.50m{\ell}/kg$ of I.J.T. dosed to the rats and checked after two and four weeks and thereby, a sinificant reduction in the volume of blood glucose was made. 2. The volume of total cholesterol of blood serum in the diabetic rats, induced by streptozotocin, was increased in comparision to the normal flock, and in the fouth week of dosing $1.25m{\ell}/kg$ of I.J.T., a significant reduction of total cholesterol volum was shown in the blood serum. 3. The volume of triglyceride in the blood serum of the diabetic rats, induced by the streptozotocin, was increased in comparision to the normal flock, and with dosing $1.25m{\ell}/kg$ and $12.50m{\ell}/kg$ of I.J.T., a significant reduction of triglyceride volium was shwon in the blood serum after second and fourth week. 4. The volume of phospholipid in the blood serum of the diabetic rats, induced by the streptozotocin, was somewhat reduced in comparision to the normal flock, and with dosing $1.25m{\ell}/kg$ of I.J.T., a significant reduction of phospholipid volium in the blood serum was shown after first and second week. 5. The volume of total protein in the blood serum of the diabetic rats, induced by the streptozotocin, was somewhat reduced in comparison to the normal flock however, with dosing the I.J.T., no significant change on the volium of total protein was made in the blood serum. 6. The volume of potassium in the blood serum of the diabetic rats, induced by the streptozotocin, was increased in comparision to the normal flock however, with dosing the I.J.T., no significant change of potassium content was made in the blood serum. 7. The volume of sodium in the blood serum of the diabetic rats, induced by the streptozotocin, was somewhat reduced in comparision to the normal flock, and with dosing $12.50m{\ell}/kg$ of the I.J.T., a significant increase was shown in the blood serum after first week. Soyangin, accompanying with a middle and upper heat, the I.J.T. - that has a merits of cleaning up fever, solving fever, solving poison, solving thirst and restoring exhaustion - can be proved that it has a blood sugar reduction effect and it is considered that it can be meritorious for strengthening vitality, further, many other exhausting diseases, and can actively used for the cases such as thirst causing disease and a disease that causes an abnormal food hunger, thirst causing and frequent going to pass urine, in particular.

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Physiolosical Activities of Mixed Extracts of Acantopancis senticosi Radicis Cortex and Eucommiae Cortex (가시오갈피 및 두충 혼합엑스의 생리활성)

  • Hwang, Wan-Kyun;Choe, Su-Bu;Kim, Il-Hyuk
    • Korean Journal of Pharmacognosy
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    • v.27 no.1
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    • pp.65-74
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    • 1996
  • The studies were conducted to investigate the anti-diabetic activities on the hyperglycemia induced by streptozotocin in rats, Anti-fatigue, Decrease of body weight activities in mouse and anti-gastric ulcer activities in stress-induced rats by Mixed Extracts of Acantopanacis senticosi Radicis Cortex and Eucommiae Cortex in Korea. 1. The blood glucose levels of streptozotocin-induced hyperglycemic rats were dose-dependently decreased by administrations of various doses(100, 200, 400, significantly 200+100mg/kg) of Mixed Extracts from Acantopanacis senticosi Radicis Cortex and Eucommiae Cortex. 2. The serum total cholesterol levels of streptozotocin-induced hyperglycemic rats were dose-dependently decreased by administrations of various doses (100, 200, 400, significantly 200+100mg/kg) of Mixed Extracts from Acantopanacis senticosi Radicis Cortex and Eucommiae Cortex. 3. The serum triglyceride levels of streptozotocin-induced hyperglycemic rats were dose-dependently decreased by administrations of various doses(100, 200, 400, significantly 200+100mg/kg) of Mixed Extracts from Acantopanacis senticosi Radicis Cortex and Eucommiae Cortex. 4. The swimming time levels in mouse were dose-dependently extended by administrations of various doses(100, 200, 400, significantly 200+100mg/kg) of Mixed Extracts from Acantopanacis senticosi Radicis Cortex and Eucommiae Cortex. 5. The body weight levels in mouse were dose-dependently decreased by administrations of various doses(100, 200, 400, significantly 200+100mg/kg) of Mixed Extracts from Acantopanacis senticosi Radicis Cortex and Eucommiae Cortex. 6. Stress-induced gastric ulcer were dose-dependently repaired by administrations of various doses(100, 200, 400, significantly 200+100mg/kg) of Mixed Extracts from Acantopanacis senticosi Radicis Cortex and Eucommiae Cortex.

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Effects of Gentiana scabra Bunge var.buergeri Max. Extract on Blood Glucose and Lipid Composition in Streptozotcin induced Hyperglycemic Rats (용담(Gentiana scabra Bunge var.buergeri Max.) 추출액이 Streptozotocin 유발 당뇨성 흰주의 혈당 및 지질 성분에 미치는 영향)

  • 김용균;김한수
    • Journal of Life Science
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    • v.8 no.4
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    • pp.381-386
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    • 1998
  • This study was designed to observe the effects of the feeding Gentiana scabra Bunge var.buergeri Max. extract on the improvement of the blood glucose, lipids in the serum of streptozotcin(55mg/kg of body weight, I.P. injection) induced hyperglycemic rats (S.D. strain, ) fed the experimental diets for 4 weeks. Concentrations of blood glucose were significantly higher in the streptozotocin administration(I.P.) groups (group 2(streptozotcin+water), 3(streptozocin+Gentiana scabra Bunge extract), 4(streptozotocin+cholesterol+water), 5(streptozotocin+cholesterol+Gentiana scabra Bunge extract) than those in the control group(group 1(water)). Bolld glucose concentrations were rather lower in the group 3(streptozotocin+Gentiana scabra Bunge extract)than in the group 2(streptozotocin+water), and then those of the group 5 were rather lower than in the group 4. There was almost little significance among the groups. concentrations of total cholesterol in serum were lower in the group 5 than those in the group 4, and the group 3 were rather lower than in the group 2. Concentrations of LDL-cholesterol in serum were significantly lower in the group 3 than those 2. In the ratio of HDL-cholesterol concentrations to total cholesterol concentrations, Gentiana scabra Bunge extract administration groups were higher percentage than the other goups. Concentrations of free cholesterol and cholesteryl ester in serum were rather lower in the groups 3,5 than the other groups, and then those of the group were higher percentage than the other groups. concentrations of free cholesterol and cholesteryl ester in serum were rather lower in the groups 3,5 than the groups, and then those of the group 3 were significantly lower than in the group 4. Concentrations of triglyceride and phospholipid in serum were decreased in the group 3 than in the other groups. The activities of aspartate aminotransferase and alanine aminotransferase in serum were increased in the all experimental groups than in the control group. From the above research, the Gentiana scabra Bunge var.buergeri Max. extracts were effective on the improvement of the lipid compositions in serum of streptozotcin induced hyperglycemic and hypercholosterolemic rats.

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An in Vivo Study of Dopamine Metabolism in Hyperglycemic Rat Striatum

  • Lim, Dong-Koo;Lee, Kyung-Min
    • Archives of Pharmacal Research
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    • v.18 no.4
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    • pp.249-255
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    • 1995
  • The changes in the levels of the extracellular dopamine metabolites and the responses to various dopamine agents were studied by using microdialysis inhyperglycemic rat striatum. The hyperglycemia were induced by the administriation of streptozotocin (40 mg/kg, i.p. for 3 days.). The basal levels of striatal dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) were significantly decreased in hyperglycemic rat striatum. After the administration ofl D-1 and D-2 receptor antagonists, SCH-23390 and (-)sulpiride, to rats 14 days after the last administration of STZ, the increased rates in DOPAC levels were higher in hyper- than in normoglycemic rats. However, after the administration of dopamine autoeceptor agonist, 3(-)PPP, the levels of the extracellular HVA were increased in normoglycemic rats, but those were not altered in hyperglycemic rats. The results indicate that the striatal dopamine activities were decreased in the hyperglycemic rats and suggest that release of dopamine may be decreased in hyperglycemic rats. Furthermore it suggest that the increase in the levels of the extracellular dopamine metabolites by dopamine antagonists might be dur to the incrrased sensitivities of the dopamine receptors in hyperglycemic state.

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Effects of KIOM-79 on streptozotocin-induced insulin-dependent diabetes in Sprague-Dawley rats (Streptozotocin을 이용한 제1형 당뇨 유발 흰쥐에서 KIOM-79의 효과)

  • Lee, Jong Hyuk;Yoon, Sang-Pil;Kim, Jin-Sook;Chang, In-Youb
    • Journal of Medicine and Life Science
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    • v.15 no.2
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    • pp.72-78
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    • 2018
  • We investigated the effect of KIOM-79, 80% ethanolic extract of herbal prescription isolated from Magnolia officinalis, Pueraria lobate, Glycyrrhiza uralensis, and Euphorbia pekinensis, on streptozotocin-induced diabetes in Sprague-Dawley rats. The rats were treated orally with KIOM-79 (500 mg/kg/day) 1) for 3 days prior to streptozotocin (60 mg/kg, intraperitoneal) injection or 2) for 9 weeks after establishing diabetes model to examine acute and chronic effects on hyperglycemia and biochemical variables, respectively. As a result, KIOM-79 had little effects on hyperglycemic changes in acute model. Sexual comparison, however, showed reduced hyperglycemia in female rats, especially 24 hours after streptozotocin injection with or without KIOM-79 pretreatment. In chronic model, streptozotocin-induced hyperglycemia was well established, but KIOM-79 treatment showed no statistically significant effects on all variables. Thus, based on our findings KIOM-79 might have little effects on streptozotocin-induced insulin-dependent diabetes although it has been known to have hypoglycemic and antidiabetic effects on non-insulin-dependent diabetes models.

The Effect of Low-Intensity Treadmill Exercise on Blood Levels of Glucose, Insulin and Lipids in Streptozotocin-Induced Diabetic Rats (저강도 treadmill 운동이 streptozotocin 유도 당뇨쥐의 혈당, 인슐린 및 지질 농도에 미치는 영향)

  • 김세종;서혜림;고정림;염종우;예정복;이선주;김경환;손원협;장은숙
    • Journal of Life Science
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    • v.14 no.2
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    • pp.245-251
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    • 2004
  • Exercise is beneficial to the diabetic patients and streptozotocin-induced diabetic rat has been used for the study of exercise effect. The purpose of this study was to establish the optimal condition of induction of hyperglycemic diabetic rat using streptozotocin and to examine the preventive effect of treadmill exercise on the diabetic rat before and after streptozotocin injection. Intraperitoneal injection of increasing amount of streptozotocin up to 40 mg/kg dose-responsively induce hyperglycemic diabetic rat and inversely reduced the blood insulin level. Body weight was also gradually reduced with the increasing amount of streptozotocin. Control and diabetic rats exercised for 4 weeks before streptozotocin injection. The exercise was performed in the treadmill for 25 minutes a day and 5 times a week with low intensity (0 degree tilt, 15 m/min velocity). Following streptozotocin injection, the blood glucose level was measured every week and the rat was sacrificed after 4 weeks to measure the concentration of insulin and blood lipids. The blood levels of glucose and insulin was significantly reduced with exercise before streptozotocin injection, while those were not changed after streptozotocin injection. The levels of blood lipids such as total cholesterol, HDL-cholesterol, LDL-cholesterol and triglyceride were close to normal control rats. From this study, researchers found the optimal condition of preparation of streptozotocin-induced hyperglycemic diabetic rat, and the mild treadmill exercise has beneficial effect on preventing hyperglycemia and hyperlipidemia. Thus, even low intensive running prevent not only diabetes but also diabetic vascular complications.

Effect of Rosa rugosa, Thunbergii to the Streptozotocin induced Hyperglycemic Rats (매괴화(玫瑰花) 잎, 줄기, 뿌리가 streptozotocin으로 유발(誘發)된 당뇨(糖尿)흰쥐에 미치는 영향(影響))

  • Kim, Hee-Chul;Kim, Jung-Sang
    • The Journal of Internal Korean Medicine
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    • v.17 no.1
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    • pp.278-289
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    • 1996
  • This paper aims to probe the effect of Rosa rugosa, Thunbergii which streptozotocin induced hyperglycemic rats was examined by glucose contents, body weight, kidney weight and histological observation. The results obtained were as follows: 1. The glucose level is decreased in the adminstered group of the Rosa rugosa compared with control group. These effects are in order named root group, stem group and leaf group group. BUN is increased fold of 1.52 in the control group compared with normal group, but the administered group of Rosa rugosa are decreased., in order named leaf group, root group and stem group. 2. The kidney hypertrophy to body weight is the higest in the control group compared with the other group in rats, and glucose contents are in order named stem group, leaf group and root group. 3. The loss of body weight is the highest in the control group, and the loss is in order named stem group, leaf group and root group in rats. 4. In histological observations, pancreatic pancreatic islets of control group shows disrupted or condensed nuclei, and the intercellular space between acini are enlarged. In the experimental group, histological damages of the acini and pancreatic islet are recovered compared with control group. Thes results suggest that the extracts of Rosa rugosa are effected to diabetes mellitus, root extract correlated with the function of kidney, and leaf extract is decreased plasma glucose.

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