• Research in Plant Disease
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• v.10 no.2
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• pp.130-134
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• 2004

The pathogenesity of 25 isolates of Colletotrichum gloeosporioides from apple, 42 isolates from pepper, 5 isolates from jujube, 8 isolates from persimmon was evaluated to know transmission to strawberry from other infected plants. Followings are the results. Colony morphology and spore size on potato dextrose agar was similar. When each isolate was inoculated on leaf and petiole of strawberry, isolates from persimmon was the most pathogenic. Five isolates, one pathogenic isolate per each host, were evaluated in simulated field condition under natural rainfall for their natural infectivity. All isolates infected strawberry in field condition, so C. gloeosporioides from other hosts are potential inoculum source of strawberry anthracnose.

• Research in Plant Disease
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• v.8 no.4
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• pp.228-233
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• 2002

This experiment was carried out to investigate the primary inoculum of strawberry anthracnose in nursery field. The pathogen, Colletotrichum gloeosporioides was not detected in soil and weeds of nursery field but symptom of anthracnose was developed in mother plants collected from market after incubation in humid chamber, The symptom of anthracnose was expressed in the strawberry plant that reserved for 17 days in field after inoculation by spore suspension but was not observed there after. When inoculated leaves were observed by SEM, only appressoria were observed 7 days after inoculation. So, it is guessed that dissemination of Colletotrichum sp. into nursery held will be by contamination of mother plants, and diagnosis by naked eyes may be impossible because symptom will be not developed if environment is to be adequate to penetration and in case of imperfect penetration after germination, the pathogen remains appressorium to achieve penetration.

• Research in Plant Disease
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• v.17 no.3
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• pp.295-301
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• 2011

The effect of various fungicides on anthracnose of strawberry, caused by Colletotrichum gloeosporioides, was determined in vitro, and in greenhouse and field trials. The $EC_{50}$ values of benomyl were clearly different between two sensitive and resistant groups of isolates. Iminoctadine tris had lower $EC_{50}$ values than mancozeb and propineb as protective fungicides and the response of mancozeb, propineb and azoxystrobin was variable depending on the isolate. In the greenhouse, pre- and post- inoculation fungicide applications significantly reduced disease compared to the non-treated control. Propineb, mancozeb and azoxystrobin were effective in controlling the disease when applied prior to inoculation. Metconazole and prochloraz-Mn treatments as ergosterol biosynthesis-inhibiting fungicides had the lowest incidence of anthracnose. In the nursery field trials in 2009 and 2010, the reduced fungicide spray program provided similar levels of disease control compared to the calendar-based applications with captan. A reduced spray program based on efficacious fungicides such as prochloraz-Mn will be useful for strawberry growers and provide more options for controlling anthracnose in Korea.

• The Plant Pathology Journal
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• v.24 no.1
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• pp.17-23
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• 2008

A total of 33 isolates of Colletotrichum species obtained from pepper, apple, and strawberry in 2001 and 2002 were identified based on mycological characteristics, responses to fungicides carbendazim and the mixture of carbendazim and diethofencarb, and nucleotide sequence analysis of internal transcribed spacer (ITS) regionMost of the Colletotrichum isolates from pepper could be identified as C. acutatum. The pepper isolates produced grey white mycelia that gradually changed to dark gray. The conidia were variable in size, and almost cylindrical in shape with at least one rounded end. They could grow on PDA amended with carbendazim or with the mixture of carbendazim and diethofencarb at $10{\mu}g/ml$, to which the isolates from apple and strawberry were very sensitive. A part of the ITS regions from the Colletotrichum isolates was amplified with the specific primers designed for C. acutatum (Ca1-1) or C. gloeosporioides (Cg1-3). A primer pair of Ca1-1 and a universal primer (ITS4) amplified a 496-bp DNA fragment from all of the pepper isolates examined and one apple isolate. Taken together, it is conclusive that the Colletotrichum isolates causing the typical lesion of anthracnose on pepper fruits are C. acutatum.

• Proceedings of the Korean Society of Life Science Conference
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• 2007.10a
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• pp.103.2-103.2
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• 2007

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• The Plant Pathology Journal
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• v.33 no.1
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• pp.80-86
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• 2017

One of the major problems in strawberry production is difficulty in diagnosis of anthracnose caused by Colletotrichum acutatum or Glomerella cingulata in latent infection stage. We here developed a diagnostic tool for the latent infection consisting of initial culturing of fungi, DNA extraction, synthesis of PCR-amplified probes and microtube hybridization (MTH) using a macroarray. The initial culturing step is convenient to lure the fungi out of the plant tissues, and to extract PCR-inhibitor-free DNA directly from fungal hyphae. For specific detection of the fungi, PCR primers were designed to amplify the fungal MAT1-2 gene. The subsequent MTH step using the PCR products as probes can replace the laborious electrophoresis step providing us sequence information and high-throughput screening. Using this method, we have conducted a survey for a few thousands nursery plants every year for three consecutive years, and finally succeeded in eliminating latent infection in the third year of challenge.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2000.10a
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• pp.36.1-36
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• 2000

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2000.10a
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• pp.12.1-12
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• 2000

• Horticultural Science & Technology
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• v.34 no.2
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• pp.242-248
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• 2016

This research was conducted to determine the effects of four different substrates, expanded rice hulls (ERH), commercial substrates for strawberries (CSS), clay sand (CS), and loamy sand (LS), on the inhibition of anthracnose crown rot (ACR) in strawberry. Mother plants of 'Seolhyang' strawberry were transplanted into an elevated bed in March, 2013 and March, 2014 and the runners connecting mother plants and daughter plants were cut in early August of both years. After separation, growth characteristics of the daughter plants were measured and then each daughter plant was inoculated with conidial suspensions of Colletotrichum fructicola, one of several species of Colletotrichum that causes ACR in strawberries. The incidence of ACR as influenced by the different substrates was investigated in both years. The daughter plants grown on CSS had the highest values for shoot height, leaf area, and fresh weight. Those grown on ERH and LS substrates also displayed good above-ground growth characteristics except for fresh weight, but the daughter plants grown on CS had the poorest above-ground growth characteristics. The ERH and CS treatments resulted in the highest number of primary roots and the greatest root weight. The CSS-grown daughter plants had the highest ACR disease index, followed by the CS and LS treatments, but there were no significant differences among the three substrates. However, the ERH-grown daughter plants had a markedly lower ACR disease index on October 11, 2013 and October 7, 2014. The CSS-grown daughter plants had high nitrogen and potassium contents and low calcium content, whereas the ERH-grown daughter plants had low nitrogen levels and high silicon levels. The results of this study provide basic information on the ability of the different substrates tested to provide disease suppression of ACR in the propagation of strawberry transplants.

• The Plant Pathology Journal
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• v.38 no.6
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• pp.673-678
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• 2022

Vapours from origanum oil (O) and thyme oil (T) were applied to the four soil-borne strawberry pathogens Fusarium oxysporum f. sp. fragariae, Colletotrichum fructicola, Lasiodiplodia theobromae, and Phytophthora cactorum, causing Fusarium wilt, anthracnose, dieback, and Phytophthora rot, respectively. Increasing T vapour doses in the presence of O vapour strongly inhibited mycelial growths of the four pathogens and vice versa. When mycelia of F. oxysporum f. sp. fragariae and P. cactorum exposed to the combined O + T vapours were transferred to the fresh media, mycelial growth was restored, indicating fungistasis by vapours. However, the mycelial growth of C. fructicola and L. theobromae exposed to the combined O + T vapours have been slightly retarded in the fresh media. Prolonged exposure of strawberry pathogens to O + T vapours in soil environments may be suggested as an alternative method for eco-friendly disease management.