• Toxicological Research
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• 제2권1호
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• pp.1-7
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• 1986

Procarcinogen induced DNA single-strand breaks and unschduled DNA synthesis were measured in primary rat hepatocytes culture. For DNA single-strand breaks assay, rat liver DNA was prelabeled by injection 3H-thymidine during the peak of DNA synthesis following partial hepatectomy. Hepatocytes were isolated from the rat 2 weeks after surgery by a collagenase perfusion techinique and maintained as monolayers in serum free medium on collagen-coated culture dishes. DNA sigle-strand breaks were measured by the alkaline elution techinique.

• 건설안전기술
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• 통권49호
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• pp.84-91
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• 2009

By bond mechanism between the prestressing strand and the concrete surrounding it, the effective force of prestressing must be transferred to the concrete entirely. The distance required to transfer the effective force of prestressing is called the transfer length, and the development length is the bond length required to anchor the strand as it resists external loads on the member. Transfer length was determined from the concrete strain profile at the level of the strands at transfer and development length was determined from various external loading lengths and compared with current code equation. Through the test results, bond failure is predicted based on the distress caused by cracks when they propagate within the transfer zone of prestressing strand. The current code equation was found to be conservative in comparison with the measured value.

• Molecules and Cells
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• 제26권3호
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• pp.308-313
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• 2008

In animals, microRNAs (miRNAs) and small interfering RNAs (siRNAs) repress expression of protein coding genes by assembling distinct RNA-induced silencing complexes (RISCs). It has previously been shown that passenger-strand cleavage is the predominant mechanism when siRNA duplexes are loaded into Argonaute2 (Ago2)-containing RISC, while an unwinding bypass mechanism is favored for miRNA duplexes with mismatches. Here I present experimental data indicating that some mammalian miRNAs are assembled into Ago2-containing RISC by cleaving their corresponding miRNA star strands. This phenomenon may depend on the secondary structure near the scissile phosphate of the miRNA duplex. In addition, I show that ATP is not required for star-strand cleavage in this process. Taken together, the data here provide insight into the miRNA-loading mechanisms in mammals.

• 한국안전학회지
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• 제23권6호
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• pp.115-121
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• 2008

By bond mechanism between the prestressing strand and the concrete surrounding it, the effective force of prestressing must be transferred to the concrete entirely. The distance required to transfer the effective force of prestressing is called the transfer length, and the development length is the bond length required to anchor the strand as it resists external loads on the member. Transfer length was determined from the concrete strain profile at the level of the strands at transfer and development length was determined from various external loading lengths and compared with current code equation. Through the test results, bond failure is predicted based on the distress caused by cracks when they propagate within the transfer zone of prestressing strand. The current code equation was found to be conservative in comparison with the measured value.

• Natural Product Sciences
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• 제8권4호
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• pp.162-164
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• 2002

The methanol extracts of 42 Korean indigenous plants were evaluated for the DNA strand-scission activity. As a result, the 17 extracts were found to be active in the criteria of $IC_{50}$}<$25\;{\mu}g/ml$. Among others, the MeOH extracts of Caesalpinia sappan and Mucuna birdwoodiana showed the most potent DNA strand-scission activity with $IC_{50}$ values of 5.9 and $4.9\;{\mu}g/ml$, respectively. Therefore, the partition and fractionation for C. sappan were performed and tested in the DNA strand-scission assay system for further bioassay-guided fractionation.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2012년도 제38회 춘계학술대회논문집
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• pp.408-410
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• 2012

본 연구에서는 수지 인장시편과 스트랜드 인장시편을 적용하여 탄소섬유/에폭시 복합재의 고온에서의 특성을 조사하였다. 연구결과에 따르면 수지시편의 인장강성은 수지의 유리전이 온도에 근접해감에 따라 서서히 감소하지만 스트랜드 인장시편의 인장강성은 상온에서의 인장강성을 유지한다. 수지시편과 스트랜드 시편의 인장강도는 온도가 유리전이 온도에 근접할수록 급격한 감소를 나타내었다.

• 한국생산제조학회지
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• 제21권4호
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• pp.619-624
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• 2012

In this study, MMT/fiber/polymer composites were fabricated by impregnating chopped strand glass mat into a vinylester resin mixed with clay. Tensile tests has been performed by using a universal testing machine to determine the effect of MMT addition on the tensile properties of MMT/chopped strand glass fiber/vinylester composites. And some pictures which are magnified cross section of breaking parts are has been taken by using a FE-SEM to confirm the behavior at breaking. The contents ratio of MMT applied in the composites were 0.5, 1.0, 1.5, and 2.0 wt% respectively. It has been found that the tensile strength and elastic modulus of MMT/chopped strand glass fiber/vinylester composites were improved at a proper content of MMT. Tensile strength and elastic modulus were maximized at a content of 1.0 wt% due to most effective dispersion of MMT. On the contrary, the failure strain was increased as MMT content was increased.

• Journal of Nutrition and Health
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• 제32권6호
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• pp.654-660
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• 1999

Folate, a precursor of the coenzyme tetrahydrofolate, plays an important role in DNA replication and cell proliferation, and thus could influence rapidly proliferating immune cells such as leukocytes and splenocytes. The effects of dietary folate on folate concentrations of plasma, thymus, spleen and leukocytes were investigated in rats. The animals were raised for 6 weeks on semipurifed experimental diets containing 0mg, 2mg, 4mg, 8mg folate/kg diet. Folate concentrations were determined microbiologically using Lactobacillus casei(ATCC 7469), and DNA strand breaks produced by alkaline treatment were analyzed fluorometrically. When compared to folate adequate diet, the folate deficient diet(0mg folate/kg diet) resulted in lowest folate levels in plasma, thymus, spleen and leukocytes, and the highest DNA strand breaks in spleen cells and leukocytes. Dietary folate levels significantly increased folate concentrations of immune tissues, leukocytes, and the plasma in a dose dependent manner, folate concentrations being highest with a diet providing 8mg folate/kg diet. The percentages of the double strand DNA remaining in the splenocytes and leukocytes after alkaline treatment were significantly increased with higher amounts of dietary folate in a dose dependent manner. Folate intakes of 8mg than 4mg/kg diet was found to be more effective in the prevention of DNA strand breaks. The results of this study suggest that increased folate above the requirement level could improve DNA stabilities in immune cells.

• BMB Reports
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• 제49권7호
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• pp.355-356
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• 2016

The THO/TREX complex consists of several conserved subunits and is required for mRNA export. In metazoans, THO/TREX binds a subset of mRNAs during RNA splicing, and facilitates their nuclear export. How THO/TREX selects RNA targets is, however, incompletely understood. In our recent study, we reported that THO is loaded onto Piwi-interacting RNA (piRNA) precursor transcripts independent of splicing, and facilitates convergent transcription in Drosophila ovary. The precursors are later processed into mature piRNAs, small noncoding RNAs that silence transposable elements (TEs). We observed that piRNAs originating from dual-strand clusters, where precursors are transcribed from both strands, were specifically affected by THO mutation. Analysis of THO-bound RNAs showed enrichment of dual-strand cluster transcripts. Interestingly, THO loading onto piRNA precursors was dependent on Cutoff (Cuff), which comprises the Rhino-Deadlock-Cutoff (RDC) complex that is recruited to dual-strand clusters by recognizing H3K9me3 and licenses convergent transcription from he cluster. We also found that THO mutation affected transcription from dual-strand clusters. Therefore, we concluded that THO/TREX is recruited to dual-strand piRNA clusters, independent of splicing events, via multi-protein interactions with chromatin structure. Then, it facilitates transcription likely by suppressing premature termination to ensure adequate expression of piRNA precursors.

• Structural Engineering and Mechanics
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• 제76권1호
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• pp.67-81
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• 2020

Pretensioned concrete (PC) is widely used in contemporary construction. Bond of prestressing strand is significant for composite-action between the strand and concrete in the transfer and flexural-bond zones of PC members. This study develops a new methodology for quantifying the bond of 18-mm prestressing strand in PC members based on results of a pullout test, the Standard Test for Strand Bond (STSB). The experimental program includes: (a) twenty-four pretensioned concrete beams, using a wide range of concrete compressive strength; and (b) twelve untensioned pullout specimens. By testing beams, the transfer length, flexural-bond length, and development length were all measured. In the STSB, the pullout forces for the strands were measured. Experimental results indicate a significant relationship between the bond of prestressing strand to the code-established design parameters, such as transfer length and development length. However, the code-predictions can be unconservative for the prestressing strands having a low STSB pullout force. Three simplified bond equations are proposed for the design applications of PC members.