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Enhanced Lipid Production of Chlorella sp. HS2 Using Serial Optimization and Heat Shock

  • Kim, Hee Su;Kim, Minsik;Park, Won-Kun;Chang, Yong Keun
    • Journal of Microbiology and Biotechnology
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    • 제30권1호
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    • pp.136-145
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    • 2020
  • Chlorella sp. HS2, which previously showed excellent performance in phototrophic cultivation and has tolerance for wide ranges of salinity, pH, and temperature, was cultivated heterotrophically. However, this conventional medium has been newly optimized based on a composition analysis using elemental analysis and ICP-OES. In addition, in order to maintain a favorable dissolved oxygen level, stepwise elevation of revolutions per minute was adopted. These optimizations led to 40 and 13% increases in the biomass and lipid productivity, respectively (7.0 and 2.25 g l-1d-1 each). To increase the lipid content even further, 12 h heat shock at 50℃ was applied and this enhanced the biomass and lipid productivity up to 4 and 17% respectively (7.3 and 2.64 g l-1d-1, each) relative to the optimized conditions above, and the values were 17 and 14% higher than ordinary lipid-accumulating N-limitation (6.2 and 2.31 g l-1d-1). On this basis, heat shock was successfully adopted in novel Chlorella sp. HS2 cultivation as a lipid inducer for the first time. Considering its fast and cost-effective characteristics, heat shock will enhance the overall microalgal biofuel production process.

계획된 행위이론에 근거한 대학생의 헌혈의도 및 헌혈행위 예측요인 (Predictive Factors on Blood donation Intention and Behavior in College Students: Base on the Theory of Planned Behavior)

  • 김유정;최인희
    • 한국산학기술학회논문지
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    • 제15권6호
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    • pp.3789-3798
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    • 2014
  • 본 연구는 Ajzen의 계획된 행위이론(TPB)에 근거하여 대학생의 헌혈의도와 헌혈행위에 영향을 미치는 요인을 파악하는 데 목적이 있다. 연구대상은 G시에 위치한 일개 대학 대학생 258명으로 하였으며, 자료 수집은 2013년 6월 3일부터 14일까지의 기간에 구조화된 자기기입식 설문조사로 하였다. 자료 분석은 SPSS/WIN 19.0을 이용하였고, 대학생의 헌혈의도에 영향을 미치는 요인은 단계별 중회귀 분석을, 헌혈행위에 영향을 미치는 요인은 로지스틱 회귀분석을 시행하였다. 대학생의 헌혈의도에 영향을 미치는 요인을 확인한 결과 헌혈에 대한 지각된 행위통제, 헌혈에 대한 태도, 헌혈에 대한 주관적 규범순이었으며, 65.6%의 영향력이 있었다. 또한, 헌혈행위에 영향을 미치는 요인을 확인한 결과 헌혈의도(OR=1.129, p=.010)만이 유의하였으며, 헌혈에 대한 지각된 행위통제는 헌혈행위에 유의한 영향을 미치지 않은 것으로 나타났다. 따라서 추후 대학생의 헌혈의도를 증가시키기 위한 프로그램에서 지각된 행위통제, 태도 및 주관적 규범을 강화하는 것은 헌혈의도를 높이고 이로써 헌혈행위를 증가시킬 수 있을 것으로 본다.

한국 남자의 연령별 골밀도에 영향을 미치는 영양요인 분석 (Nutritional Factors Related to Bone Mineral Density in the Different Age Groups of Korean Men)

  • 유춘희;이정숙;이일하;김선희;이상선;강순아
    • Journal of Nutrition and Health
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    • 제37권2호
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    • pp.132-142
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    • 2004
  • Nutritional factors affecting bone mineral density (BMD) in the different age groups of Korean men were investigated to obtain baseline data for maintaining bone health. Information on diet and anthropometry was collected in 80 elementary school children, 83 high school students, 87 adults aged 25 to 35 years and 98 elderly people over 60 years of age. Data for food and nutrient intake were obtained by 24-hour recall method. BMDs of lumbar spine (L$_2$- L$_4$) and femoral neck were measured by dual energy x-ray absorptiometry. The relationship between BMD and nutritional factors were analyzed. In the femoral neck, 5.7% of adults was classified as osteopenia and 47.9% and 37.8% of the elderly were classified as osteopenia and osteoporosis. It was shown that plant protein, Ca, p, Fe, thiamin, riboflavin and vitamin C intakes were related with BMD in all age groups. As for the mean adequacy ratio (MAR) of nutrients, the lowest quartile group of BMD showed significantly lower MAR among children. The RDA percent of nutrients was a strong influential factor on BMD. Subjects who consumed below 75% of Korean RDA in energy, vitamin A, thiamin, and vitamin C showed lower BMD. Stepwise multiple regression analysis revealed that MAR in children, vegetable Ca in adolescents, and vitamin C in adults and elderly people were the highest influential factor on BMD. Therefore, the above results demonstrated that not only calcium but also other nutrients such as protein, iron, vitamin A, riboflavin, and vitamin C were necessary in order to keep the healthy bone status. In addition, although there were various dietary factors that influenced bone density, MAR was identified as the major factor that affected bone density. Thus, a balanced diet that includes all nutrients is necessary for a healthy bone density. (Korean J Nutrition 37(2) : 132-142, 2004)

점착특성을 갖는 내열 폴리이미드/폴리실록산 이중층 필름 제조 연구 (Preparation of Thermostable Polyimide/Polysiloxane Double Layered Films with Pressure-sensitive Adhesion Property)

  • 권은진;정현민
    • 폴리머
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    • 제38권4호
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    • pp.544-549
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    • 2014
  • 이중층 필름 구조로서 상부에 폴리실록산 층과 하부에 폴리이미드 층을 갖는 내열 점착 필름을 제조하였다. 이중층 필름제조 과정에서 폴리실록산이 용해된 tetrahydrofuran(THF) 용액이 폴리이미드 층 상부에 도포된 이후, 상온~$80^{\circ}C$ 온도범위에서 에이징(aging) 과정을 거쳐 두 층 사이에서 나노 상분리에 의한 도메인이 500 nm 두께의 중간층으로 형성되었고 이에 대한 모폴로지는 투과전자현미경을 통해 조사되었다. 이러한 중간층 형성을 통해 상부 폴리실록산은 균일하고 안정적 층을 형성함으로 재현성 있는 점착특성을 나타내었으며, $300^{\circ}C$ 처리에서도 8-13 g/inch의 점착성질을 나타내었다. 또한 이중층 폴리이미드/폴리실록산과 나노 도메인 중간층 구조를 갖는 필름은 안정된 단일 박막으로 얻어지며 $435^{\circ}C$의 높은 열분해 온도를 가지고, $300^{\circ}C$에서 점착특성이 유지되는 결과를 보여 마이크로일렉트로닉스의 공정 조건에 적합한 활용 가능성을 보였다.

간호대학생의 의사소통 능력과 대인불안 (Interpersonal Communication Competence and Social Anxiety in Nursing Students)

  • 황선경;이윤지
    • 한국간호교육학회지
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    • 제21권4호
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    • pp.518-527
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    • 2015
  • Purpose: This study aimed to identify a relationship between the communication competence and social anxiety in nursing students. Methods: With a cross-sectional survey, a convenience sampling of 303 nursing students was taken from P-university in Y-city. The instrument for the study was a questionnaire that is consisted of the general characteristics, an Interaction Anxiousness Scale (IAS) and a Global Interpersonal Communication Competence Scale (GICC). Data was analyzed using descriptive statistics, t-test, one-way ANOVA, Kruskal-Wallis, Pearson's correlation coefficients and stepwise multiple regression with SPSS version 21.0. Results: The mean score of the IAS was $41.89{\pm}8.74$ and the GICC was $3.51{\pm}0.34$. Communication competence significantly differed by the satisfaction of major (F=10.86, p<.001). In addition, the satisfaction of interpersonal relationships was significantly different in both communication competence (F=17.52, p<.001) and social anxiety (F=23.85, p<.001). Social anxiety had a negative correlation with communication competence (r=-.48, p<.001). Social anxiety accounted for 55.3% of the variance -social relaxation (${\beta}$=-.62), assertiveness (${\beta}$=-.18), interaction management (${\beta}$=.14) and efficiency (${\beta}$=-.13)- of communication competence's subcategories. Conclusion: It was found that communication competence has an influence on social anxiety. Therefore, education programs are needed to promote communication competence for a decline of social anxiety in nursing students.

관상동맥질환자의 질병관련 지식, 자기효능감, 사회적 지지가 환자역할행위에 미치는 영향 (Influences of Knowledge, Self-efficacy, and Social Support on Sick Role Behavior in Patients with Coronary Artery Disease)

  • 김순희;이선희
    • 한국보건간호학회지
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    • 제28권2호
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    • pp.228-240
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    • 2014
  • Purpose: This study was conducted in order to determine the influences of knowledge, self-efficacy, and social support on sick role behavior in patients with coronary artery disease. Methods: The participants were 192 patients with coronary artery disease in D city. Data were collected from January 1, 2014 to April 30, 2014 using a questionnaire. Data analysis was performed using SPSS/WIN 19.0 using ANOVA, t-test, Pearson correlation, and stepwise multiple regression. Results: Factors having an influence on sick role behavior were age (70 years old ${\beta}$=.275, p<.001), occupation (Office job ${\beta}$=-.230, p<.001), knowledge (${\beta}$=.204, p<.001), self-efficacy (${\beta}$=.188, p<.001), underlying disease (stroke ${\beta}$=.142, p=.008), and family type (live with their children ${\beta}$=-.128, p=.038). These factors explained 56% of the variance in sick role behavior. Conclusions: More positive knowledge and self-efficacy are needed for the sick role behavior. In addition, results of the study indicated that age, occupation, underlying disease, and family type were very important in predicting health behaviors of patients with coronary artery disease. Thus, it would be necessary to include patients' characteristics in designing an education program for people with coronary artery disease.

.Net Framework를 이용한 영어 이러닝 시스템 (English E-Learning System Based on .NET Framework)

  • 전수빈;정인범
    • 한국정보통신학회논문지
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    • 제16권2호
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    • pp.357-372
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    • 2012
  • 대부분의 이러닝 시스템은 복잡한 가입 절차를 거쳐야 하고 교육과정 또한 여러 부분으로 나뉘어 있어 서비스를 이용하기가 쉽지 않다. 이는 사용자가 학습에 대한 관심을 쉽게 잃게 만들 수 있고 학습 능률도 떨어뜨릴 수 있는 문제점을 가지고 있다. 또한 대부분의 이러닝 과정은 유아 및 초등학생들을 대상으로 하고 있지 않아 보호자 없이는 학습을 원활이 할 수 없는 설정이다. 따라서 본 논문에서는 초등학생들을 대상으로 하는 영어 이러닝 시스템을 설계 및 구현 한다. 제안하는 시스템은 사용자들의 나이가 어리다는 것을 참고하여 사용자 측면의 환경을 최대한 쉽고 흥미롭게 구성 한다. 본 시스템은 3가지의 클래스로 구성되고 사용자들은 능력에 맞게 미리 정해진 코스 정보를 이용하여 각 클래스별로 코스를 진행하게 된다.

학령기 비만아동의 자기효능감과 건강증진 생활양식과의 관계연구 (A Study on the Relationship between Self-Efficacy and Health Promoting Lifestyle Profile of Childhood Obesity)

  • 박경숙;오숙희
    • Child Health Nursing Research
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    • 제10권2호
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    • pp.173-179
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    • 2004
  • Purpose: The purpose of this study was to offer basic data concerning the prevention of obesity for obese children to identify the relationship between their self-efficacy and the degree of performance on health promoting lifestyle profile. Method: The subjects were 148 fourth to sixth grade elementary school students in Gwang-ju, Korea during the period from September 10 to September 25, 2001. The collected data were analyzed by mean, standard deviation, t-test, ANOVA, Pearson's Correlation Coefficient and Stepwise Multiple Regression by using the SPSS program. Result: The relationship between general characteristics of subjects and self-efficacy were significant differences according to eating time(F=5.338, p=.006). The relationship between general characteristics of subjects and health promoting lifestyle profile were significant differences according to grade(F=5.615, p=.005), eating time(F=8.591, p=.000), concern about weight control(F=3.915, p=.022), experience of weight control(t=7.632, p=.006). Health promoting lifestyle profile was showed significant positive correlations with self-efficacy(r=.537, p=.000). Self-efficacy(27.6%) was the highest factor predictor health promoting lifestyle profile of obesity students. In addition to eating time, concern about weight control, explained for 33.3% in health promoting lifestyle profile. Conclusion: Results of this study point to self-dfficiency as a major factor in the health promoting behavior of childhood obesity. According to the results of this study, There is a reason to develop a health program to promote self-efficiency.

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만성관절염 환자의 건강증진행위와 통증, 자아존중감, 가족지지 및 자기효능감과의 관계 (The Relationship among The Health Promoting Behavior, Pain, Self-esteem, Family Support and Self-efficacy in Patients with Chronic Arthritis)

  • 오현아;김종임
    • 근관절건강학회지
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    • 제11권1호
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    • pp.50-60
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    • 2004
  • Purpose: The purpose of this study was to examine the relationships among the health promoting behavior, pain, self-esteem, family support, self-efficacy in patients with chronic arthritis. Method: The data for this study were collected from February 19, 2003 to April 7, 2003. The subjects were 150 chronic arthritis patients who visited University Hospital in D City. The research instruments used in this study were HPLP II, Visual analogue scale, Self-esteem scale, Family support scale, Self-efficacy scale. In data analysis, SPSS PC ver 10.0 program was utilized and data were analyzed using descriptive statistics and t-test, ANOVA, Multiple Stepwise Regression and Pearson's correlation coefficient. Result: The average score of the pain, self-esteem, family support, self-efficacy, health promoting behavior was 5.38, 3.37, 4.29, 70.30, 2.68, respectively and the variable with the highest degree of it's subscale was interpersonal support(3.08), whereas the one with the lowest degree was exercise(1.66). There was a positive correlation(r=.327, p=.000) between the self-esteem and health promoting behavior. There was a positive correlation(r=.540, p=.000) between the family support and the self-efficacy and health promoting behavior. Family support, self-efficacy and marital status were significant factors to explain a variance of health promoting behavior in patients with chronic arthritis(51.6%). health promoting behavior. There was a positive correlation(r=.477, p= .000) between Conclusion: The health promoting behavior were positively correlated with self-esteem, family support and self-efficacy. These findings help to understand relationships among self-esteem, family support and self-efficacy in chronic arthritis patients. In addition, family support, self-efficacy and marital status were the mainly influencing factors of health promoting behavior. Among these variables family support was the most significant factor to predict a health promoting behavior. According to the results of this study, family support must be considered as a main factor in the nursing strategy for health promoting behavior of chronic arthritis patients.

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최종이용자탐색시스템의 이용과 이용자의 인지적 특성간의 관계 연구 (A Study of Users' Cognitive Characteristics Influencing upon the Usage of End-User Searching Systems)

  • 이상복
    • 한국문헌정보학회지
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    • 제27권
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    • pp.291-339
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    • 1994
  • The purpose of this study is to find personal characteristics that affect users' cognitive characteristics of system, and to verify correlations between this users' cognitive characteristics and selection of system usage in using end -user searching systems (EUSS), For corroborative analysis of this study, preliminary model was constructed referring to Davis' Technology Acceptance Model. The model consists of exogenous variables (personal characteristics) , parameter variables (perceived usefulness, perceived ease of use), and effect variables (selection of system usage), When exogenous variables affect parameter variables, exogenous variables are independent variables and parameter variables are dependent variables. In addition, in correlation of parameter variables, which have been affected by exogenous variables, with effect variables, parameter variables are independent variables and effect variables are dependent variables, As for the research methodology, this study regards the Academic Information System connected with the Internet as EUSS, So questionnaires have been sent to researchers in universities who were conducting direct searching for the system. 229 valid responses to questionnaires have been analyzed according to Pearson Correlation Analysis and Stepwise Selection of Multiple Regression in the statistical software packages, 'SPSS PC+'. The findings and conclusions made in this study are summarized as follows; 1. Among the personal characteristics (age, disciplinary, computer literacy level, perceived usefulness of use education and training, perceived satisfaction of end-user searching, perceived satisfaction of system characteristics), all characteristics but age affect perceived usefulness and perceived ease of use. Specifically, perceived satisfaction of end user searching and perceived satisfaction of system characteristics most affect perceived usefulness and perceived ease of use respectively. 2. Perceived usefulness and perceived ease of use have a direct effect on selection of system usage in using EUSS. 3, Perceived usefulness more affect selection of system usage than perceived ease of use in using EUSS.

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