• Title/Summary/Keyword: stepwise

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The Successful Process of Technology Commercialization Training Program : Focus on the Absorptive Capacity (기술사업화 교육프로그램의 성공적인 프로세스 개발 연구 - 흡수역량을 중심으로-)

  • Kim, Myung-Seuk;Choi, Jong-In
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.12 no.6
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    • pp.2506-2514
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    • 2011
  • The research object is stepwise process of absorptive capacity is important factor to get the successful result of technology commercialization training program. We analyze the relationship between absorptive capacity and educational performance with empirical study to the students who attended the technology commercialization training program. Results show that potential absorptive capacity has positive effect on realized absorptive capacity. Realized absorptive capacity also has the positive effect on educational performance. We conclude the successful technology commercialization training program is based on the stepwise process of absorptive capacity. That means technology commercialization training program will set up based on not functional but stepwise process. Further more, technology commercialization training program must be practical, focusing, interdisciplinary, and repetitive courses.

Stepwise Synthesis of Quercetin Bisglycosides Using Engineered Escherichia coli

  • Choi, Gyu Sik;Kim, Hyeon Jeong;Kim, Eun Ji;Lee, Su Jin;Lee, Youngshim;Ahn, Joong-Hoon
    • Journal of Microbiology and Biotechnology
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    • v.28 no.11
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    • pp.1859-1864
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    • 2018
  • Synthesis of flavonoid glycoside is difficult due to diverse hydroxy groups in flavonoids and sugars. As such, enzymatic synthesis or biotransformation is an approach to solve this problem. In this report, we used stepwise biotransformation to synthesize two quercetin bisglycosides (quercetin 3-O-glucuronic acid 7-O-rhamnoside [Q-GR] and quercetin 3-O-arabinose 7-O-rhamnoside [Q-AR]) because quercetin O-rhamnosides contain antiviral activity. Two sequential enzymatic reactions were required to synthesize these flavonoid glycosides. We first synthesized quercetin 3-O-glucuronic acid [Q-G], and quercetin 3-O-arabinose [Q-A] from quercetin using E. coli harboring specific uridine diphopsphate glycosyltransferase (UGT) and genes for UDP-glucuronic acid and UDP-arabinose, respectively. With each quercetin 3-O-glycoside, rhamnosylation using E. coli harboring UGT and the gene for UDP-rhamnose was conducted. This approach resulted in the production of 44.8 mg/l Q-GR and 45.1 mg/l Q-AR. This stepwise synthesis could be applicable to synthesize various natural product derivatives in case that the final yield of product was low due to the multistep reaction in one cell or when sequential synthesis is necessary in order to reduce the synthesis of byproducts.

Elasto-plastic Analysis and In-situ Measurement on Rock Behaviors with Stepwise Excavation of the Steep Soft Seam at a Great Depth (심부 급경사 연약층의 채굴 진행에 따른 주변 암반 거동의 탄소성 해석 및 현장계측)

  • 정소걸;신중호
    • Tunnel and Underground Space
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    • v.14 no.4
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    • pp.295-303
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    • 2004
  • For the deep underground excavation site with the geological complexity of soft seam and hard rock, the numerical analysis and in-situ measurement on the behaviors of roadway and surrounding rock according to stepwise excavation of the steep soft seam are carried out. The strata behavior is modeled using elasto-plastic FEM considering the empirical failure criteria of Hoek & Brown and the strain-softening model. Hydraulic pressure capsule, MPBX and tape extensometer are installed around the roadway for the in-situ measurement of rock stress and deformation. Despite the complexity of geology and excavation procedure, the elasto-plastic analysis considering the empirical failure criteria of Hoek & Brown and the strain-softening model shows good agreement with the in-situ measurement. Comparison of numerical modeling with in-situ measurement enables to predict the behaviors of the roadway and to obtain design parameters for the excavation and support at depth.

A Study on the Ego-Resilience and Social Adaptability of the children and Adolescence in Institutional Care (시설보호아동$\cdot$청소년의 자아탄력성과 사회적 적응에 관한 연구)

  • Hyun Eun-Min;Park Hea-Young
    • Journal of Families and Better Life
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    • v.23 no.1 s.73
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    • pp.19-29
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    • 2005
  • The purpose of this study was to investigate ego-resilience and social adaptability of the children and adolescents in institutional care. The study also investigated the protective factors and the risk factors on ego-resilience and social adaptability and their relationship. The subjects in this study were 140 children and adolescents in institutional care. The research data were analyzed using Frequency, Pearson's Correlation, Stepwise Multiple Regression Analysis and T-Test in SPSS WIN 10.0 program. The major findings were as follows. First, ego-resilience of the children and adolescents in institutional care was related to locus of control, depression and anxiety and unsatisfaction of institutional care. Result of stepwise multiple regression analysis showed that depression and anxiety and locus of control in the order had significant effects on ego-resilience. Second, social adaptability of the children and adolescents in institutional care was related to social support and parent-child bond. Unsatisfaction of institutional care and social support in the order had significant effects on social adaptability by stepwise multiple regression analysis. Third, the children and adolescents who had higher level of ego-resilience reported better social adaptability. The overall research results revealed that the risk factors such as unsatisfaction of institutional care and depression and anxiety than the protective factors had more significant effects on ego-resilience and social adaptability of children and adolescents in institutional care. This finding suggested that elimination of risk factors rather than development of protective factors was a more important task for the welfare of children and adolescents in institutional care.

Stepwise Addition Technology for the High Yield Production of Chitosanoligosaccharide (키토산올리고당 생산을 위한 다단계 첨가방법)

  • Lee, Ki-Sun;Kim, Seung-Mo;Lim, Hyun-Soo
    • KSBB Journal
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    • v.14 no.6
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    • pp.639-642
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    • 1999
  • Optimization of the chitosanoligosaccharide production was studied with chitosanase. The optimum conditions for the enzymic reaction have been determined. Enzyme stability was maintained above 90% after 6 days at pH 5.0. The optimum initial reaction rate was appeared in 1.0% of chitosan solution. The production yield of chitosanoligosaccharides was over at 0.4%~2.0% of chitosan. At 4.0% of chitosan solution, however, the production yield was decreased to 64.6%. To increase the yield, stepwise addition of substrate into the reactor was investigated. In this case, the yield was increased from 64.6% to 83.2% and the final concentrations of chitosanoligosaccharide was 12.26 mg/mL. By TLC analysis, most of the chitosanoligosaccharides produced were 3-5 mers.

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Design of a Stepwise Enrichment Curriculum for the Gifted and Talented in Informatics (정보과학영재를 위한 단계별 심화 교육과정의 설계)

  • Kim, KyungKyu;Lee, JongYun
    • The Journal of Korean Association of Computer Education
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    • v.17 no.1
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    • pp.35-50
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    • 2014
  • The current curriculums for the gifted and talented students in informatics are not practical and systematic in teaching methods because it is hard to provide the gifted and talented students with appropriate education individualized at their own levels. Furthermore, there are still some problems in previous curriculums for the gifted and talented students in informatics as follows. First, there are not sufficient on previous curriculums for the gifted and talented education in informatics in middle and high schools. Second, previous curriculums in use do not contribute to cultivating the creativity, problem-solving, logical thinking abilities and insight for the gifted students. Third, there is no curriculum, which reflects on individual's ability and aptitude, for the gifted and talented education in informatics. Therefore, in this paper, we suggest stepwise enrichment curriculums that enables the gifted and talented students to be equipped with creative problem-solving skills and computational thinking abilities. In this regard, at first, The problem with existing research and educational institutes for the gifted and talented in informatics were analyzed. Next we developed a concrete stepwise enrichment curriculum in accordance with the practical educational objectives of the gifted and talented in informatics.

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Kinetic Study on SNAr Reaction of 1-Y-Substituted-phenoxy-2,4-dinitrobenzenes with Hydroxide Ion: Effect of Substituent Y on Reactivity and Reaction Mechanism

  • Kang, Tae-Ah;Cho, Hyo-Jin;Um, Ik-Hwan
    • Bulletin of the Korean Chemical Society
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    • v.35 no.7
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    • pp.2135-2138
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    • 2014
  • A kinetic study is reported for the SNAr reaction of 1-Y-substituted-phenoxy-2,4-dinitrobenzenes (1a-1h) with OH- in 80 mol % $H_2O$/20 mol % DMSO at $25.0{\pm}0.1^{\circ}C$. The second-order rate constant ($k_{OH^-}$) increases as the substituent Y in the leaving group changes from an electron-donating group (EDG) to an electronwithdrawing group (EWG). The Br${\o}$nsted-type plot for the reactions of 1a-1h is linear with ${\beta}_{lg}$ = -0.16, indicating that the reactivity of substrates 1a-1h is little affected by the leaving-group basicity. A linear Br${\o}$nsted-type plot with ${\beta}_{lg}=-0.3{\pm}0.1$ is typical for reactions reported previously to proceed through a stepwise mechanism in which formation of a Meisenheimer complex is the rate-determining step (RDS). The Hammett plot correlated with ${\sigma}_Y{^{\circ}}$ constants results in a much better correlation than that correlated with ${\sigma}_Y{^-}$constants, implyng that no negative charge is developing on the O atom of the leaving group (or expulsion of the leaving group is not advanced at all in the TS). This excludes a possibility that the $S_NAr$ reaction of 1a-1h with $OH^-$ proceeds through a concerted mechanism or via a stepwise pathway with expulsion of the leaving group being the RDS. Thus, the current reactions have been concluded to proceed through a stepwise mechanism in which expulsion of the leaving group occurs rapidly after the RDS.

Kinetic Study on Aminolysis of Phenyl 2-Pyridyl Carbonate in Acetonitrile: Effect of Intramolecular H-bonding Interaction on Reactivity and Reaction Mechanism

  • Song, Ji-Hyun;Lee, Jae-In;Um, Ik-Hwan
    • Bulletin of the Korean Chemical Society
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    • v.35 no.7
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    • pp.2081-2085
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    • 2014
  • Second-order rate constants ($k_N$) have been measured spectrophotometrically for the reactions of phenyl 2- pyridyl carbonate (6) with a series of cyclic secondary amines in MeCN at $25.0{\pm}0.1^{\circ}C$. The Br${\o}$nsted-type plot for the reaction of 6 is linear with ${\beta}_{nuc}$ = 0.54, which is typical for reactions reported previously to proceed through a concerted mechanism. Substrate 6 is over $10^3$ times more reactive than 2-pyridyl benzoate (5), although the reactions of 6 and 5 proceed through the same mechanism. A combination of steric hindrance, inductive effect and resonance contribution is responsible for the kinetic results. The reactions of 6 and 5 proceed through a cyclic transition state (TS) in which H-bonding interactions increase the nucleofugality of the leaving group (i.e., 2-pyridiniumoxide). The enhanced nucleofugality forces the reactions of 6 and 5 to proceed through a concerted mechanism. In contrast, the corresponding reaction of 4-nitrophenyl 2-pyridyl carbonate (7) proceeds through a stepwise mechanism with quantitative liberation of 4-nitrophenoxide ion as the leaving group, indicating that replacement of the 4-nitrophenoxy group in 7 by the PhO group in 6 changes the reaction mechanism (i.e., from a stepwise mechanism to a concerted pathway) as well as the leaving group (i.e., from 4-nitrophenoxide to 2-pyridiniumoxide). The strong electron-withdrawing ability of the 4-nitrophenoxy group in 7 inhibits formation of a H-bonded cyclic TS. The presence or absence of a H-bonded cyclic TS governs the reaction mechanism (i.e., a concerted or stepwise mechanism) as well as the leaving group (i.e., 2-pyridiniumoxide or 4-nitrophenoxide).

Effects of Empowerment and Regarding Standard Precautions for Healthcare associated Infection Control in Healthcare Workers on Performances (의료종사자의 임파워먼트와 의료관련감염 관리 표준주의지침 인지도가 수행도에 미치는 요인)

  • Jung, Mi-Ra
    • Journal of Digital Convergence
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    • v.16 no.2
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    • pp.243-250
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    • 2018
  • The purpose of this study was to examine relationships among empowerment, awareness and performance on standard precautions and identify the factors that affect performance of standard precaution in the health care workers. The data were collected from 200 health care workers working in the general hospital located Y city. The data were analyzed by descriptive statistics, t-test, ANOVA, Scheffe, Pearson's correlation coefficient, and stepwise multiple regression. The result of the stepwise multiple regression indicates the awareness predict 49.0% (F=191.98, P<.001) in the performance of standard precaution. Therefore, it is necessary to develop awareness of the standard precaution strategy program for improving the performance of standard precautions in health care workers.

A Multivariate Analysis of Korean Professional Players Salary (한국 프로스포츠 선수들의 연봉에 대한 다변량적 분석)

  • Song, Jong-Woo
    • The Korean Journal of Applied Statistics
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    • v.21 no.3
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    • pp.441-453
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    • 2008
  • We analyzed Korean professional basketball and baseball players salary under the assumption that it depends on the personal records and contribution to the team in the previous year. We extensively used data visualization tools to check the relationship among the variables, to find outliers and to do model diagnostics. We used multiple linear regression and regression tree to fit the model and used cross-validation to find an optimal model. We check the relationship between variables carefully and chose a set of variables for the stepwise regression instead of using all variables. We found that points per game, number of assists, number of free throw successes, career are important variables for the basketball players. For the baseball pitchers, career, number of strike-outs per 9 innings, ERA, number of homeruns are important variables. For the baseball hitters, career, number of hits, FA are important variables.