• FLOWER RESEARCH JOURNAL
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• v.17 no.3
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• pp.147-151
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• 2009

The research was carried out a solution to prevent the breakage of stem resulted from stem cavity in the Dendranthema grandiflorum Ramat. 'Baekma'. Stem cavity occurred in the lower parts of stem for about 5 cm long as the stem grew to 20 cm, and the breakage increased to about 60 cm as the stem grew to 80 cm. Plant height, stem diameter, and flower stalk length were the highest in the planting density of $11cm{\times}11cm$. The light interception by plants increased as the planting density decreased (47, 99 and 143%). The strength and hardness values were the greatest, $567kg{\cdot}cm^{-2}$ and $1,339kg{\cdot}cm^{-2}$ in the planting density of $11cm{\times}11cm$. Plant height and plant weight increased in the foliage fertilization of one time, but the plants in the control flowered earlier. The strength and hardness increased in the foliage fertilization treatments and the greatest in the treatment of one time application of $60mg{\cdot}L^{-1}$ Silicate fertilization. The Si content of stems increased as the fertilization concentration and treatment time increased. The Si content in $30mg{\cdot}L^{-1}$ silicate treated twice was two times as high as that of the control.

• Journal of Veterinary Clinics
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• v.28 no.1
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• pp.63-70
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• 2011

To consider the iliac fossa as the vascular anastomosis site of kidney transplantation for the short-term study of acute rejection in pigs. Twelve domestic pigs weighing 39~48 kg underwent heterotopic renal allgraft transplantation. The experimental animals were divided into 2 groups in terms of renal vascular anastomosis site; the external iliac artery and vein were used in iliac fossa model (n = 6), the abdominal aorta and the caudal vena cava inferior to the kidney were used in abdominal cavity model (n = 6). Renal function was evaluated by daily measurement of plasma creatinine and BUN concentrations. The experiments' health including postoperative complications was also assessed daily for 8 days after transplantation. After euthanazation gross and histopathologic analysis was performed. All six pigs in iliac fossa model developed neuropraxia and lameness of the ipsilateral pelvic limb. However, no necrosis was observed in any pigs. In the abdominal cavity model, durations of both the surgical operation and the vascular anastomosis were significantly longer than those in the iliac fossa model. Furthermore, ischemia injury of the transplanted kidney was increased in abdominal cavity model, which induced accelerated-acute immune response from day 4 after transplantation. Despite of pelvic limb complication, the iliac fossa model showed more advantages including not only less ischemia time related to easy vascular anastomosis, but also less immune response during the acute rejection period. The results indicate that the iliac fossa model may be appropriate to the study of acute rejection in porcine kidney transplantation.

• FLOWER RESEARCH JOURNAL
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• v.17 no.4
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• pp.251-255
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• 2009

This study was conducted to investigate effect of ionic strength and feeding times of a nutrient solution on reduction of stem cavity size and improvement of flower quality in chrysanthemum 'Baekma'. A nutrient solution was applied with different strengths at three stages, namely, transplanting, budding, and flowering. The solution EC was adjusted as 1.61.82.0, 1.81.81.8, 1.82.01.8, and $2.02.02.0dS{\cdot}m^{-1}$ in four treatments. Feeding frequency per a day were 4 times for 12 min., 8 times for 6 min., 12 times for 4 min., and 18 times for 2.7 min. each. Cut flower length as affected by different strengths of a nutrient solution was the greatest in the plot of EC $2.02.02.0dS{\cdot}m^{-1}$. However number of leaves, stem diameter, and leaf size were greater in EC $1.82.01.8dS{\cdot}m^{-1}$ than in other treatments. Also, petal number of petals was the greatest and stem cavity size was the smallest in the plot of EC $1.81.81.8dS{\cdot}m^{-1}$. Plant height, number of leaves, stem diameter, leaf size were greater in the plot with 12 times feed ing per a day. number of petal was most in the plot with 8 times feeding per day, while stem cavity size was the smallest in the plot with 12 times feed ing per a day. Therefore, the better plant growth, the smaller stem cavity size.

• Journal of Korean Neurosurgical Society
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• v.61 no.1
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• pp.10-18
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• 2018

Objective : To investigates the effect of curcumin on proliferation of spinal cord neural stem/progenitor cells (SC-NSPCs) and functional outcome in a rat spinal cord injury (SCI) model. Methods : Sixty adult male Sprague-Dawley rats were randomly and blindly allocated into three groups (sham control group; curcumin treated group after SCI; vehicle treated group after SCI). Functional recovery was evaluated by the Basso, Beattie, and Bresnahan (BBB) scale during 6 weeks after SCI. The expression of SC-NSPC proliferation and astrogliosis were analyzed by nestin/Bromodeoxyuridine (BrdU) and Glial fibrillary acidic protein (GFAP) staining. The injured spinal cord was then examined histologically, including quantification of cavitation. Results : The BBB score of the SCI-curcumin group was better than that of SCI-vehicle group up to 14 days (p<0.05). The coimmunoreactivity of nestin/BrdU in the SCI-curcumin group was much higher than that of the SCI-vehicle group 1 week after surgery (p<0.05). The GFAP immunoreactivity of the SCI-curcumin group was remarkably lower than that of the SCI-vehicle group 4 weeks after surgery (p<0.05). The lesion cavity was significantly reduced in the curcumin group as compared to the control group (p<0.05). Conclusion : These results indicate that curcumin could increase the expression of SC-NSPCs, and reduce the activity of reactive astrogliosis and lesion cavity. Consequently curcumin could improve the functional recovery after SCI via SC-NSPC properties.

• Horticultural Science & Technology
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• v.29 no.4
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• pp.298-305
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• 2011

This study was conducted to enhance the stem firmness of standard chrysanthemum 'Baekma' bred in Korea for commercial quality improvement and inhibition of stem breaking during transportation through foliar spray with calcium agents. Calcium agent screening 'Baekma' was examined using $CaCl_2{\cdot}2H_2O$, $Ca(NO_3)_2{\cdot}4H_2O$, and OS-Ca (natural liquid calcium compounds extracted from oyster shell) depending on each concentration (0, 0.001, 0.01, 0.1, and 1.0%, respectively). All calcium agents sprayed with 1.0% caused chemical injury such as stem bending or leaf burn. OS-Ca also showed more sensitive response to chemical injury than the other calcium agents because OS-Ca was absorbed very well by 'Baekma' leaves. Maximum stem firmness measured during the final harvest was greater in OS-Ca than in the other calcium agents. Especially, maximum stem firmness was greatest in 0.01% OS-Ca. However, elastic strength and maximum bending stress were greater in 0.001% OS-Ca than in the others. Thus, OS-Ca ranged from 0.005 to 0.05%, which did not show any chemical injury, was finally selected as the first candidate for hardening the stem of 'Baekma'. The next experiment using OS-Ca was conducted with the concentrations of 0, 0.005, 0.01, and 0.05%, respectively. From the results, 0.05% OS-Ca showed better plant growth and parameters such as plant height, stem diameter (upper and middle part), the number of leaves, and dry weights of each part than the other concentrations of OS-Ca and control. As for stem firmness depending on OS-Ca concentration, the Ca content within stem, maximum firmness, elastic strength, and maximum bending stress of stem in 'Baekma' sprayed with 0.05% OS-Ca showed the highest values among all the treatments and it turned out to be very high level of significance between control and OS-Ca treatments. However, the area and percentage of the inside cavity within horizontal stem section in 'Baekma' did not show any significance between any treatments including control. Thus, stem firmness of 'Baekma' did not show any correlation with the inside cavity area of stem. In conclusion, we recommend foliar sprays with 0.05% OS-Ca at vegetative growth stage to enhance stem firmness of 'Baekma' during transportation.

• FLOWER RESEARCH JOURNAL
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• v.18 no.4
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• pp.238-243
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• 2010

This study was conducted to investigate effects of nutrient solution pH control agent and foliar spray of ethephon on the reduction of stem inside-cavity and improvement of flower quality in chrysanthemum 'Baekma'. Changes of pH in nutrient solution as affected by the kind of pH control agent showed more settled pattern in the plot of KOH treatment than in others. Plant growth and development such as cut flower length, leaf numbers, cut flower weight and petal number were the greatest in the plot of KOH. However, the size and area of stem cavity was large in KOH rather than $KHCO_3$. As changes in the absorbing patterns of mineral elements as affected by nutrient solution pH control agent in closed system, contents of total nitrate, phosphorus, potassium and calcium by $KHCO_3$ was absorbed into the plant less than KOH, so that it remained a lot of mineral element residues rather than KOH in closed system. Plant growth as affected by the foliar spray of ethephon showed growth retardation effect in the plot of solution diluted to 1 : 500 and growth promotion effect in the plot of solution diluted to 1 : 1,000 or 1 : 2,000. The number of petals was the best in the plot of foliar spray of ethephon solution diluted to 1 : 1,000 before flowering at 45 days showing 331 petals compared to control showing 302 petals. The size and area of stem cavity as affected the foliar spray of ethephon was smaller 1 mm and 7%, respectively, in the plot of solution diluted to 1 : 2,000 before flowering at 30 days than in control. Therefore, treating pH stabilization using KOH after floral initiation stage with the foliar spray of ethephon solution diluted to 1 : 2,000 before flowering at 30 days would help to reduce stem cavity size and improve flower quality in hydroponically grown chrysanthemum 'Baekma'.

• Proceedings of the KSAR Conference
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• 2004.06a
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• pp.275-275
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• 2004

To evaluate the potential of the stem cell therapy as a method for prenatal management of spinal open neural tube defect (ONTD), the influence of embryonic stem cells injected into the amniotic cavity on the re-closure capacity of spinal ONTD was investgated. Spinal neural tube was incised open for a length of 6 somites using chick embryos of Hamburger and Hamilton stage 18 or 19. (omitted)

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2006.05a
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• pp.651-652
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• 2006

The choice of suitable hip implant is one of important factors in total hip replacement (THR). In clinical view points, improper adaptation of hip implant might cause abnormal stress distribution to the bone, which can shorten the lifespan of replaced hip implant. Currently, interest in custom-designed hip implants has increased as studies reveals the importance of geometric shape of patient's femur in modeling and designing custom hip implants. In this study, we have developed the custom-designed hip implant models with various sizes in hip implant, and the stress distribution in the bone was analyzed using Finite Elements methods. It was found that minimizing the gap between implant stem and femoral cavity is crucial to minimize stress concentration in the bone.

• Korean Journal of Medicinal Crop Science
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• v.4 no.3
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• pp.255-260
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• 1996

This study was conducted to investigate the characteristics of aboveground plants and red ginseng of polystem ginseng in 6 years of age having two or more stems in a plant. Total leaf weight and area of polystem ginseng were larger, while its stem diameter, and the leaf weight and area of the big­gest stem in each plant were decreased with increase the stem numbers in a plant. The ratio of shoot weight to root weight in the polystem ginseng with three or more stems was higher than that in the monos­tem ginseng and the polystem ginseng with two stems, In ginseng plants with no more than 2 stems, there were positive correlations between root weight and total leaf weight, and leaf area, but not between leaf weight and area of the biggest stem. Inner cavity and inner white, limiting factors for redginseng quality grade, occurred more in tri-stem ginseng than mono- and di-stem one. Percentages of Heaven (1st grade) and Earth (2nd grade) red ginseng in tri-stem ginseng were decreased compared with mono stem and di-stem ginseng.

• Proceedings of the KSAR Conference
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• 2003.06a
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• pp.77-77
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• 2003

Chimeric animals are referred to as an organism composed of tissues derived from more than one species. In order to examine if a pluripotency of embryonic stem cells can cross the limitation of a species, we tried to establish human-mouse chimeric animals. Human embryonic stem cells were genetically modified to express eGFP using eukaryonic expression vector pcDNA 3.1 (In Vitrogene) for an easy identification. After selection with neomycin, approximately 15 cells were implanted into mouse blastocoele cavity. Ten chimeric blastocysts were transferred to one of the uterine horn of 2.5 days pesudopregnent ICR female. Out of 272 blastocysts transferred to pseudopregnant recipients 20 live newborn were obtained after 20 days. When newborn were obtained, pups were quickly removed immersed into 4% PFA. By histological examination using fluorescent microscope, green fluorescence was observed from the liver, heart, and spleen in newborn mice. Three weeks after born, presence of eGFP sequence within mouse genome (tail and kidney) was reconfirmed by PCR. eGFP sequence was amplified from the progenies of the animal suggesting a genetic transmission of the transgene. These chimeric mice having human cells at the beginning of development, are expected to recognize human cells as “self”, therefore, human cells or tissues will be able to escape the immunological surveillance of the host if grafted into the animal. These animals will serve as a good model system for studying the graft rejection in tissue transplantation and the potential of the cells to work well in many human disease.