• Toxicological Research
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• 제7권2호
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• pp.151-155
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• 1991

Thirty six crude drug samples have been prepared from different parts of twenty five plants belonging to different families, and antimutangenic activities were studied by using SOS chromotest (E. coli PQ 37). The following crude extracts of PNG medicinal plants which had a appreciable antimutagenic activity against mitomycin C were: Artocarpus communis (stem bark), Cycas circinalis (leaves), Merremia peltata (leaves), Intsia palembanica (leaves), Annona muricata (stem bark), and Artocarpus altilis (root bark).

• 생명과학회지
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• 제11권4호
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• pp.328-334
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• 2001

Male Sprageu-Dawely rats fed a cholesterol diet (Control group) or cholesterol diet supplemented with the water-soluble extract of stem bark from Cudrania tricuspidata(CTSB group) at the level of 0.5% for 2 weeks. Concentration of triglyceride in serum of CTSB group was significantly lower than that of control group. However, the other lipids concentrations of liver and serum, body weight gain, and food intake did not show significant difference between CTSB group and control group. Antioxidative activities of water-soluble extract from stem bark of Cudrania tricuspidata on the lipid peroxidation in rat were also studied in vivo by measuring the formation of thiobarbituric acid reactive substance(TBARS). Concentration of TBATS in the liver homogenates of CTSB group was significantly higher than that in control group. Nonheme iron concentration was significantly increased in the liver of CTSB group compared to control group. suggested that enhanced nonheme irom was associated with enhanced peroxidation of liver in CTSB group. These results suggested that water soluble extract from stem bark of Cudrania tricuspidata exert the hypotriglycerolemic effect and might amplify the lipid peroxidation of tissues in cholesterol fed rats.

• Natural Product Sciences
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• 제15권4호
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• pp.208-212
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• 2009

The heartwood of Rhus verniciflua Stokes (Anacardiaceae) is widely used for a medicinal plant to treat diabetes mellitus and lingering intoxication in the folkloric society of Korea, while the stem bark has been traditionally used to treat menstrual disorder and helminthiasis. We previously reported that a flavonoid, fustin, isolated from the heartwood of R. verniciflua is effective in Alzheimer‘s disease and rheumatoid arthritis. To explore the possibility to produce more flavonoid-rich fraction from this plant, the MeOH extracts from the plant parts of stem bark, outer heartwood, and inner heartwood were quantitatively analyzed by HPLC. Phenolic or flavonoid compounds (chlorogenic acid, caffeic acid, p-coumaric acid, sulfuretin, fustin, fisetin, luteolin and astragalin) were detectable in the HPLC chromatogram. The orange-colored inner heartwood was found to contain the highest levels of fustin (16.96 mg/g) and sulfuretin (2.22 mg/g). Moreover, the inner heartwood accumulated fustin and sulfuretin at least 4-fold higher level as compared to the stem bark and outer heartwood. The levels of total phenolic compounds positively correlated with the extents of antioxidant properties. Therefore, the inner heartwood of R. verniciflua could be used to increase fustin concentration of the extract which is capable of treating Alzheimer‘s disease and rheumatoid arthritis.

• Advances in Traditional Medicine
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• 제3권4호
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• pp.205-211
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• 2003

Stem bark of Cinchona sp. (Rubiaceae) is one of the well known drugs for its therapeutic values in traditional as well as modern medicine. Even though a lot of work has been carried out on quinoline alkaloids of Cinchona, its phenolic constituents received very little attention. In the present study, we evaluated antioxidant properties of C. officinalis stem bark methanolic extract and water extract containing phenolic compounds (total phenolics 21.37, 5.18% w/w respectively in the two extracts) in different in vitro and ex vivo models viz., antiradical activity by DPPH reduction, superoxide radical scavenging activity in riboflavin/light/NBT system, nitric oxide radical scavenging activity in sodium nitroprusside/Greiss reagent system and inhibition of lipid peroxidation induced by iron-ADP-ascorbate in liver homogenate and haemolysis of erythrocytes induced by phenylhydrazine in erythrocyte membrane stabilization study. Both the extracts exhibited very good antioxidant activity in all the models tested. The phenolic compounds including tannins present in the stem bark seem to offer protection from the oxidative damage.

• 한국자원식물학회지
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• 제23권5호
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• pp.406-414
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• 2010

본 연구는 제주도에 자생하는 차나무과 식물을 대상으로 식품소재 또는 생약으로의 활용 방안을 모색하고자 angiotensin I converting enzyme(ACE) 저해활성, aminopeptidase N(APN) 저해활성 및 $\alpha$-amylase 저해활성을 조사하고, 항산화활성을 검색하고 TLC를 이용하여 분석하였다. ACE 저해활성은 후피향나무(수피)와 비쭈기나무(잎)에서 50% 이상의 저해활성을 보였으며, APN 저해활성은 비쭈기나무(잎과 수피)와 후피향나무(수피)에서만 양의 활성을 보였다. $\alpha$-amylase 저해활성은 동백나무(열매), 우묵사스레피나무(수피), 후피향나무(수피)와 차나무(줄기)에서 30% 이상의 저해활성을 보였다. 항산화활성은 비쭈기나무(수피), 후피향나무(수피), 차나무(잎)에서 30% 이상의 다소 높은 전자공여능을 나타내었다. 특히, 비쭈기나무(수피)는 dot-blot test에 의해 다른 종에 비해 활성이 높아 $1.25\;{\mu}g/ml$의 낮은 농도에서도 높은 항산화활성을 보였다. TLC 분석에 의해 비쭈기나무(수피)에서 EGC(Rf 0.26) 활성이 높았으며, 비쭈기나무, 우묵사스레피나무, 후피향나무의 수피에서 EGCG(Rf 0.09) 활성이 높게 검출되었다. 그리고, 표준 catechin류와는 다른 것으로 보이는 5개의 밴드(Rf 0.54, 0.46, 0.44, 0.16, 0.03)는 Folin-Ciocalteu Reagent 방법과 Ferric chloride-alcohol 방법을 이용하여 polyphenol류인 것으로 추정되었다. 이상의 결과를 토대로 사스레피나무를 제외한 차나무과 식물들은 생리활성이 높아 식품 소재 또는 생약으로의 활용이 가능할 것으로 보이며, 활성성분의 분리 및 동정 그리고 이들 물질을 이용한 임상실험 등 보다 심도있는 연구가 필요할 것으로 보인다.

• Advances in Traditional Medicine
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• 제3권1호
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• pp.1-7
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• 2003

Some Korean plants were found to inhibit HIV-1 protease activity. The extracts of Acanthopanax koreanum (stem bark), Berchemia berchemiaefolia (stem), Berchemia berchemiaefolia (bark), Distylium racemosum (leaves), Distylium racemosum (stem), Lindera erythrocarpa (leaves), Physalis alkekengi var. francheti (root), Platycarya strobilacea (stem), Rodiola rosea (root), Rosa davurica (stem), Syringa dilatata (leaves), Viburnum awabuki (stem) and Viburnum awabuki (leaves) showed significant inhibitory effect against HIV-1 protease. Camelliatannin H from Camellia japonica and uvaol from Cratagus pinatrifida were potent active inhibitors of HIV-1 protease with $IC_{50}$ values of $0.9\;{\mu}M$ and $5.5\;{\mu}M$, respectively. The cure and prevention of AIDS have been a global challenge since it was discovered in the ealy 1980s. However, the development of anti-HIV agent that can effectively treat or prevent this disease are still demanded.

• Natural Product Sciences
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• 제10권5호
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• pp.207-210
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• 2004

Three chalcones and a stilbenoid have been isolated from the stem bark of Lindera erythrocarpa. They were elucidated as 2'-hydroxy-3',4',5',6'- tetramethoxychalcone, 2',4'-dihydroxy-3',6'-dimethoxy chalcone, 2',4',5',6'- tetrahydroxy-3'-methoxychalcone, and 5,6-dihydroxy-2,3,4-trimethoxystilbene. Chemical structures were elucidated on the basis of MS, $^1H,\;^{13}C$ and 2D-NMR spectroscopic data. This is the first report on the isolation of these compounds from the L. erythrocarpa.

• Archives of Pharmacal Research
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• 제27권3호
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• pp.283-286
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• 2004

The methylene chloride soluble fraction of MeOH extract from the stem bark of Styrax japonica S. et Z. (Styracaceae) showed significant cytotoxicity by SRB method against five human tumor cell lines. Four known pentacyclic triterpenoids, oleanolic aldehyde acetate (1), erythrodiol-3-acetate (2), euphorginol (3), and anhydrosophoradiol -3-acetate (4) were isolated by activity-guided fractionation. Their structures were determined by chemical and spectral analysis. Compounds 1-4 were isolated from S. japonica for the first time.

• 생약학회지
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• 제29권3호
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• pp.163-168
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• 1998

Seven antioxidative compounds were isolated from chloroform and ethyl acetate extracts of the stem bark of Eucalyptus globulus (Myrtaceae). They were identified as rhamnazin (1), rhamnetin (2), naringenin (3), eriodictyol (4), quercetin (5), taxifolin (6) and dihydrokaempferol-3-rhamnoside (7) on the basis of various spectroscopic analyses. These compounds inhibited lipid peroxidation with $IC_{50}$ values of 0.08-30 ${\mu}g/ml$.

• Current Research on Agriculture and Life Sciences
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• 제30권2호
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• pp.110-114
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• 2012

In the present study, acetone extract and sub-fractions of Albizia amara stem bark were evaluated for their free radical potential. The results showed that the crude extract and all the fractions exhibited antioxidant and free radical scavenging activities under different in vitro assays. Among the different fractions, the ethyl acetate fraction exhibited higher DPPH and ABTS radical scavenging activities than the standard quercetin. A. amara stem bark might be valuable source of natural antioxidants that could be used for medicinal and food applications.