• Title/Summary/Keyword: stem and root

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Histological and Morphological Characteristics of New and Latent Bud Formation in Panax ginseng C.A. Meyer (인삼의 신아 및 잠아발생의 조직 형태적 특성)

  • 정찬문;정열영
    • Journal of Ginseng Research
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    • v.19 no.3
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    • pp.281-286
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    • 1995
  • This experiment was conducted to obtain the basic information on new- and latent-bud formation, and stem vestige arrangement on the rhizome of Panax ginseng C.A. Meyer. Latent buds emerged from meristematic region between shoot and root of the embryo, and new buds for the next year were distributed both at the bottom portion of the stem and the rhizome. In the new buds, organs such as leaf, stem, and flower bud were already completely differentiated, while the latent bud had an undifferentiated meristematic tissue arranged linearly in a vertical line, indicating that each year new- and latent-buds are formed successively. This result suggests that the number of stem vestige may be used for the determination of ginseng age. Key words Rhizome, new-bud, latent-bud, histology, morphology, stem vestige, vestige arrangement.

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Accumulated Concentration of Lead in Plant Organ of Arabidopsis thaliana Exposed to Lead (납에 노출된 애기장대의 식물기관에 축적된 납 농도)

  • Park, Jong-Bum
    • Journal of Life Science
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    • v.17 no.10
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    • pp.1414-1418
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    • 2007
  • This study was to examine the accumulated concentration of lead in the organ of Arabidopsis thaliana grown in the soil added three different concentrations of lead. The accumulated concentrations of lead in the stem of plant grown in the soil added official standard concentration of lead of pollutant exhaust notified by the Ministry of Environment (1 mg/l), concentration ten times higher than the official standard concentration (10 mg/l) and concentration fifty times higher (50 mg/l) were similar to the rate of increase between three different concentrations, and increased average 24% compared with normal plant stem. The accumulated concentrations of lead in the leaf of plant grown in the soil added three different concentrations of lead were increased average 57% compared with normal plant leaf. And accumulated concentrations of lead in the leaf was no significant difference according to increase of lead concentration added in the soil as stem, the rate of increase was similar to between three different concentrations. The accumulated concentrations of lead in the root of plant grown in the soil added official standard concentration of lead of pollutant exhaust and concentration ten times higher were increased average 114% compared with normal plant root, but increased about 861% in the concentration fifty times higher than the official standard concentration. This result contrast with the data of stem and leaf. The accumulated concentration of lead in the plant body of Arabidopsis thaliana was increased according to increase of lead concentration added in the soil. Especially, the accumulated concentration of lead in the plant body grown in the concentration fifty times higher than the official standard concentration was increased about 2.6 times than normal plant. These results show that lead contaminated within the soil was more accumulated in the root than the stem or leaf, and accumulated concentrations of lead in the stem and leaf were not increased in proportion to the concentration of lead in the soil, but very increased in proportion to the concentration in the root.

Regenerative Endodontic Treatment (임상가를 위한 특집 1 - 재생 근관 치료)

  • Jung, Il Young
    • The Journal of the Korean dental association
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    • v.51 no.10
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    • pp.542-550
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    • 2013
  • The immature teeth with apical periodontitis present considerable challenges to clinicians. Therefore, new treatment protocols have been suggested to overcome the problems encountered in traditional methods. Regenerative treatment (revascularization) is one of such methods. Many case reports on the revascularization of infected immature teeth have been published, and in most of them, immature teeth with even a periapical abscess continued root formation after the disinfection of the root canal system. We now believe that this continued root formation is not an exceptional incident. As a result, it appeared that apexification has been giving way to a revascularization technique, which is a new option, in treating necrotic immature teeth. These new methods appear to be based on the healing potential of stem cells. The potential of healing or regeneration of stem cells, which are located around teeth, seems to be greater than we thought before. This review summarizes the current techniques for considering regenerative endodontic treatment procedures in treating the immature permanent tooth with pulp necrosis.

Structural Components of Cell Wall in Various Parts of Korean Ginseng(Panax ginseng C. A. Meyer) (고려인삼의 부위별 세포벽 구성성분)

  • 정영륜;박찬수
    • Journal of Ginseng Research
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    • v.7 no.2
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    • pp.169-171
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    • 1983
  • The structural components of cell wall in various parts of Korean ginseng were analysed. Pectin was abundant in the cortex and epidermis of root, and leaf. Hemicellulose in root was more than in other parts of ginseng. Cellulose content of stem and seed coat was much higher compared to other parts and lignin content was highest in seed coat.

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Physiological Response of Tetrapleura tetraptera (Schum. and Thonn.) Taub. to Soil Textural Class, Moisture and Light Intensity

  • Akinyele, Adejoke O.;Wakawa, Lucky Dartsa
    • Journal of Forest and Environmental Science
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    • v.33 no.2
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    • pp.97-104
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    • 2017
  • Investigation was carried out on response of Tetrapleura tetraptera (Schum. and Thonn.) to soil, water and light with the view of its domestication and introduction to different ecological regions. The experiment was arranged in a factorial experiment of $3{\times}3{\times}3$ in a completely randomized design (CRD) with three replicates. The factors were: soil textural class (Loamy sand, Sand and Sandy clay loam), watering regime (daily, twice a week and once a week) and light intensity (100%, 75% and 50%). Soil textural classes had significant influence on collar diameter, stem height, number of leaflets, root/shoot ratio and relative growth rate of Tetrapleura seedlings. Seedlings grown on loamy sand recorded the highest mean value- 2.28 mm for collar diameter, stem height- 12.9 cm, number of leaflets- 19.9, chlorophyll b- $0.34mg\;mL^{-1}$, leaf relative water content- 27.4% and relative growth rate- $0.037mg\;g^{-1}\;day^{-1}$. Watering regime had significant influence on the collar diameter of Tetrapleura. Seedlings watered daily recorded the highest mean value- 2.25 mm for collar diameter. Light intensity significantly influenced collar diameter and root/shoot ratio. Seedlings exposed to 100% light intensity recorded higher mean value for collar diameter- 2.28 mm and root/shoot ratio- 1.481 cm. The interaction between soil textural class and light intensity significantly affected collar diameter, stem height and number of leaflets. Higher mean value for collar diameter (2.47 mm) stem height (13.25 cm) and number of leaflets (21.16) were recorded while the interaction between soil textural class, light intensity and watering regime was significant for only number of leaflets. Tetrapleura exhibited some level of tolerance to different soil texture, drought and light intensity. Therefore, Tetrapleura has the potentials to be raised in different ecological zones characterized by difference in soil, rainfall and amount of sunshine.

Antioxidative Activities of Methanol Extracts from Different Parts of Chrysanthemum zawadskii (구절초의 부위별 메탄올 추출물의 항산화활성)

  • Chung, Hai-Jung;Jeon, In-Sook
    • Food Science and Preservation
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    • v.18 no.5
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    • pp.739-745
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    • 2011
  • The major objective of this study was to investigate the antioxidant activities of methanolic extracts from different parts (flower, leaf stem, and root) of Chrysanthemum zawadskii by employing various in-vitro assay systems. The extraction yields from the flower, leaf stem, and root were 18.347, 12.93, and 11.33-----, respectively. The total polyphenol content was highest in the flower (17.16 mg/100 g) and lowest in the root (11.33 mg/100 g). The antioxidant activities were raised within creasing amounts of extracts, and the extracts from the flower showed the highest effect on the superoxide anion radical scavenging, metal chelating on ferrous ions and reducing power. In addition, the leaf stem also showed good antioxidant activity in various systems. These results suggest that the methanolic extracts from the flower and leaf stem possess excellent antioxidant activities and may thus serve as potential sources of natural antioxidants.

Effect of Crude Extracts from Allium spp. on Growth of Several Crop Seedling (파속 식물의 조추출물이 몇가지 작물의 유묘생육에 미치는 영향)

  • 최상태;안형근;장영득
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.5
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    • pp.526-534
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    • 1996
  • This study was conducted to find out the effects of crude extracts from welsh onion, onion, chinese chives and garlic on the seedling growth of chrysanthemum, lettuce, rice, radish, chinese cabbage, cucumber and oriental melon. The crude extracts from Allium spp. inhibited the growth of chrysanthemum and lettuceseedlings at low concentration, the effect increased as the concentration grew higher, and especially in lettuce, the radicle growth was inhibited greater than the hypocotyl growth.The root extracts from welsh onion, chinese chives, and stem-leaf extracts from welsh onion, chinese chives and garlic significantly promoted the root growth of rice seedlings at 300∼700ppm and at 500∼1,000ppm, respectively, and the leaf sheath was elongated at low concentration of all extracts. The stem-leaf extracts from garlic and onion promoted the root and hypocotyl growth of chinese cabbage up to 2,000ppm or 3,000ppm. Root extracts from all Allium spp. promoted the elongation of chinese cabbage root up to 300∼700ppm, but they didn't influence its hypocotyl elongation. At low concentration of onion, garlic and chinese chives extracts, the growth of radish seedlings was highly promoted. The promotive effect, however, declined as the concentration increased. Only, extracts of chinese chives promoted the growth of radish seedling up to 5,000ppm. The stem-leaf extracts from onion and garlic promoted hypocotyl growth in pepper, up to 5,000ppm, but had little effect on other treatments. All of the extracts markedly promoted both root(main and lateral root) and hypocotyl growth in cucumber seedlings. A higher degree of promotion was made in the cucumber lateral root by onion and garlic extracts. The seedling growth of oriental melon was slightly increased by low concentration, but high concentration inhibited the root and hypocotyl growth.

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Oxidative Stress in the Cell and Antioxidant Activity of Kalopanax Pictus Extracts (음나무 추출물의 세포 내 산화 스트레스와 항산화 활성)

  • Kim, Sea-Hyun;Park, Youngki;Jang, Yong-Seok;Han, Jingyu;Chung, Hun-Gwan
    • Journal of the Korean Wood Science and Technology
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    • v.35 no.6
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    • pp.126-134
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    • 2007
  • This study reviewed the application of an extract from Kalopanax pictus stem bark and root bark as natural antioxidants. To investigate the effect on cell toxic level against transformed mouse fibroblast L929 in formula added with various extracts from Kalopanax pictus stem bark and root bark, this experiment was carried out by in vitro cytotoxicity method. Using DCFA-DA method, oxidative stress in cell was measured with other antioxidant activity methods including DPPH assay and NBT assay. Active oxygen inhibition rate for root bark insoluble hot water extracts showed the highest with 57.9% for 15 min treatment. In DPPH and NBT test, antioxidant activities of methanol extract from stem bark and insoluble hot water extract from stem bark were 96% (at 0.1%) and 95% (at 0.5%), respectively.

Potential Reasons for Prevalence of Fusarium Wilt in Oriental Melon in Korea

  • Seo, Yunhee;Kim, Young Ho
    • The Plant Pathology Journal
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    • v.33 no.3
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    • pp.249-263
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    • 2017
  • This study aims to examine the potential reasons for the current prevalence of the fusarium wilt in the oriental melon. Twenty-seven Fusarium isolates obtained from oriental melon greenhouses in 2010-2011 were identified morphologically and by analysis of elongation factor-1 alpha gene (EF-$1{\alpha}$) and internal transcribed spacer (ITS) rDNA sequences as 6 Fusarium species (8 isolates of F. oxysporum, 8 F. commune, 5 F. proliferatum, 3 F. equiseti, 2 F. delphinoides, and 1 F. andiyazi), which were classified as same into 6 EF-$1{\alpha}$ sequence-based phylogenetic clades. Pathogenicity of the Fusarium isolates on the oriental melon was highest in F. proliferatum, next in F. oxysporum and F. andiyazi, and lowest in the other Fusarium species tested, suggesting F. proliferatum and F. oxysporum were major pathogens of the oriental melon, inducing stem rots and vascular wilts, respectively. Oriental melon and watermelon were more susceptible to F. oxysporum than shintosa and cucumber; and cucumber was most, oriental melon and watermelon, medially, and shintosa was least susceptible to F. proliferatum, whose virulence varied among and within their phylogenetic subclades. Severe root-knot galls were formed on all the crops infected with Meloidogyne incognita; however, little indication of vascular wilts or stem and/or root rots was shown by the nematode infection. These results suggest the current fungal disease in the oriental melon may be rarely due to virulence changes of the fusarium wilt pathogen and the direct cause of the severe root-knot nematode infection, but may be potentially from other Fusarium pathogen infection that produces seemingly wilting caused by severe stem rotting.

Occurrence of Root Rot caused by Fusarium fujikuroi on Adzuki Bean in Korea

  • Min Sun Ha;Hyunjoo Ryu;Sung Kee Hong;Ho Jong Ju;Hyo-Won Choi
    • The Korean Journal of Mycology
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    • v.50 no.4
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    • pp.319-329
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    • 2022
  • In July 2020, wilting symptoms were observed among adzuki bean plants (Vigna angularis var. angularis L.) in the fields in Yeosu, Korea. Infected plants showed yellowing of leaves, browning inside the stems, splitting of stem bark, and wilting. When these plants were uprooted, their roots were found to be brown. The fungal pathogens NC20-737, NC20-738, and NC20-739 were isolated from symptomatic stem and root tissues. These pathogens were identified as a Fusarium fujikuroi species complex based on their morphological characteristics. Molecular identification was performed using the DNA sequence of translation elongation factor 1 alpha and the RNA polymerase II second largest subunit regions. The nucleotide sequences of all three isolates were similar to the F. fujikuroi reference isolates NRRL 13566 and NRRL 5538 of the National Centre for Biotechnology Information GenBank. A pathogenicity test was conducted by the soil inoculation method with cornmeal sand inoculum. Approximately 3 weeks after inoculation, symptoms were observed only in the inoculated adzuki bean seedlings. To the best of our knowledge, this is the first report of Fusarium root rot caused by F. fujikuroi in adzuki beans, both in Korea and worldwide.