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Microbial Assessment of Wild Cabbage and its Control (양배추의 미생물 오염도 평가 및 제어)

  • Cho, Joon-Il;Kim, Keun-Sung;Bahk, Gyung-Jin;Ha, Sang-Do
    • Korean Journal of Food Science and Technology
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    • v.36 no.1
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    • pp.162-167
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    • 2004
  • In this study, untreated (UT), water soaking (WT), and sanitizing solutions [chlorine at 100 ppm (CL): ethanol at 10% (ET); hydrogen peroxide at 1% (HP); chlorine at 100 ppm + ethanol at 10%(CE); chlorine at 100 ppm + hydrogen peroxide at 1% (CH); ethanol at 10% + hydrogen peroxide at 1% (EH); chlorine at 100 ppm + ethanol at 10% + hydrogen peroxide at 1% (CEH)] were compared in terms of their antimicrobial effectiveness against natural microflora of wild cabbage (Brassica oleracea var. capitata). All samples were kept in sanitizing solutions for 2 min, and effectiveness of sanitizing agents was evaluated based on number of decimal reduction of total aerobic mesophilic, total coliforms, E. coli, lactic acid bacteria, and yeast and mold counts. Average initial levels of these organisms in samples were $9.21{\pm}0.15,\;6.60{\pm}0.06,\;6.08{\pm}0.03,\;and\;3.66{\pm}0.08\;log_{10}\;CFU/g$ for total aerobic mesophilic bacteria, total coliforms, lactic acid bacteria, and yeasts and molds, respectively, Escherichia coli was not detected in any tested samples. Decimal reduction of populations of total aerobic mesophilic, total coliforms, E. coli, lactic acid bacteria, and yeasts and molds were: in $WT\;8.09,\;5.36,\;5.82,\;and\;3.57 log_{10}\;CFU/g;\;in \;CL\;7.39,\;4.10\;5.24,\;2.45\;log_{10}\;CFU/g;\;in\;ET\;6.78,\;4.23,\;5.20,\;2.50\;log_{10}\;CFU/g;\;in\;HP\;6.11,\;4.27,\;5.28,\;2.46\;log_{10}\;CFU/g;\;in\;CE\;6.18,\;4.26,\;5.31,\;2.49\;log_{10}\;CFU/g;\;in\;CH\;6.10,\;3.77,\;5.33,\;2.46\;log_{10}\;CFU/g;\;in\;EH\;6.07\;3.82,\;4.76,\;2.41\;log_{10}\;CFU/g;\;and\;in\;CEH\;5.27,\;3.45,\;4.45,\;2.15\;log_{10}\;CFU/g,$ respectively. Statistical analysis of the results showed effectiveness of CEH sanitizing solution for elimination of microbial contamination was the highest among all sanitizer treatments.

Medicolegal Problems in Pediatric Area (소아과 영역에서 의료분쟁)

  • Kwon, Soo Jeong;Jang, Ji Young;Kim, Nam Su;Yum, Myung Kul;Seol, In Joon;Jung, Ku Won
    • Clinical and Experimental Pediatrics
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    • v.48 no.8
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    • pp.813-819
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    • 2005
  • Purpose : Medicolegal problems start when the patient asserts the mistake of doctor and doctor does not accept it. The purpose of this study is to assess the actual condition of medicolegal problems and to provide solutions of medicolegal problems in the pediatric field. Methods : There is not official statistical data about medicolegal problems in our country. We gathered data of legal insurance program of Korean Medical Association(KMA) and court cases and other fragmentary data. Results : Between 1981 and 1995, of total 2,338 cases reported to legal problem insurance program of KMA, most common ones were 748 cases of obstetrics and gynecology. Pediatric case was ranked at the 5th, 74 cases(3.1%). According to analysis of 41 medicolegal cases' after 1990, maltreatment of patient had the highest incidence of 14 cases, injection and medication were related to 12 cases, misdiagnosis was 9 cases, patient management were related to 4 cases, and others were 2 cases. The trial result of the medicolegal cases was that 31 cases were compensated, and 8 cases were defeated, and 2 cases were still in the process. Conclusion : The aspect of medical legal problem has the tendency of radicalism and systematization. This brings an economic destitution in the patient and gives damage to a doctor. In order to reduce medicolegal problem, doctor should offer a duty of explanation and efforts to his best to satisfy patient and endeavor to make an intimate doctor-patient relationship.

Bioequivalence of Burophil Capsule to Surfolase Capsule (Acebrophylline 100 mg) (설포라제 캡슐(아세브로필린 100 mg)에 대한 부로필 캡슐의 생물학적 동등성)

  • Cho, Hea-Young;Park, Eun-Ja;Kang, Hyun-Ah;Kim, Se-Mi;Park, Chan-Ho;Oh, In-Joon;Lim, Dong-Koo;Lee, Myung-Hee;Lee, Yong-Bok
    • Journal of Pharmaceutical Investigation
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    • v.35 no.3
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    • pp.179-185
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    • 2005
  • Acebrophylline is a compound produced by salifying ambroxol with theophylline-7 -acetic acid. After acebrophylline administration, the salt splits into these two components which feature a peculiar pharmacokinetic behavior, an adequate ambroxol and a low theophylline-7-acetic acid serum levels. The purpose of the present study was to evaluate the bioequivalence of two acebrophylline capsules, Surfolase (Hyundai Pharm. lnd. Co., Ltd.) and Burophil (Kuhnil Pharm. Co., Ltd.), according to the guidelines of the Korea Food and Drug Administration (KFDA). The release of ambroxol from the two acebrophylline formulations in vitro was tested using KP VIII Apparatus II method with various dissolution media (pH 1.2, 4.0, 6.8 buffer solution and water). Twenty eight healthy male subjects, $23.25{\pm}1.43$ years in age and $64.82{\pm}6.77$ kg in body weight, were divided into two groups and a randomized $2{\times}2$ cross-over study was employed. After two capsules containing 100 mg as acebrophylline were orally administered, blood was taken at predetermined time intervals and the concentrations of ambroxol in serum were determined using HPLC with electrochemical detector (ECD). The dissolution profiles of two formulations were similar at all dissolution media. In addition, the pharmacokinetic parameters such as $AUC_t$, $C_{max}$ and $T_{max}$ were calculated and ANOVA test was utilized for the statistical analysis of the parameters using logarithmically transformed $AUC_t$, $C_{max}$ and untransformed $T_{max}$. The results showed that the differences between two formulations based on the reference drug Surfolase, were -1.64, -3.33 and -0.92% for $AUC_t$, $C_{max}$ and $T_{max}$, respectively. There were no sequence effects between two formulations in these parameters. The 90% confidence intervals using logarithmically transformed data were within the acceptance range of log 0.8 to log 1.25 $(e.g., \;log\;0.93{\sim}log\;1.05\;and\;log\;0.88{\sim}log\;1.05$ for $AUC_t$, and $C_{max}$, respectively). Thus, the criteria of the KFDA bioequivalence guideline were satisfied, indicating Burophil capsule was bioequivalent to Surfolase capsule.

Bioequivalence of SCD Zaltoprofen Tablet to Soleton® Tablet (Zaltoprofen 80 mg) (솔레톤 정(잘토프로펜 80 mg)에 대한 삼천당잘토프로펜 정의 생물학적동등성)

  • Kang, Hyun-Ah;Park, Sun-Ae;Kim, Dong-Ho;Kim, Hwan-Ho;Yun, Hwa;Kim, Kyng-Ran;Yoo, Hee-Doo;Park, Eun-Ja;Cho, Hye-Young;Lee, Yong-Bok
    • Journal of Pharmaceutical Investigation
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    • v.36 no.3
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    • pp.209-215
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    • 2006
  • Zaltoprofen, (2-(10,11-dihydro-10-oxodibenzo[b,f]thiepin-2-yl)propionic acid) is an NSAID with powerful anti-inflammatory effects as well as an analgesic action on inflammatory pain. The purpose of the present study was to evaluate the bioequivalence of two zaltoprofen tablets, $Soleton^{\circledR}$ (CJ Corp.) and SCD Zaltoprofen (Samchundang Pharmaceutical Co., Ltd.), according to the guidelines of the Korea Food and Drug Administration (KFDA). The release of zaltoprofen from the two zatoprofen formulations in vitro was tested using KP Vlll Apparatus ll method with various dissolution media. Twenty six healthy male subjects, $23.2{\pm}2.26$ years in age and$64.7{\pm}8.08$ kg in body weight, were divided into two groups and a randomized $2{\times}2$ cross-over study was employed. After a single tablet containing 80 mg as zaltoprofen was orally administered, blood samples were taken at predetermined time intervals and the concentrations of zaltoprofen in serum were determined using HPLC with UV detector. The dissolution profiles of two formulations were similar in all tested dissolution media. The pharmacokinetic parameters such as $AUC_t$, $C_{max}$ and $T_{max}$ were calculated, and ANOVA test was utilized for the statistical analysis of the parameters using logarithmically transformed $AUC_t$, $C_{max}$ and untransformed $T_{max}$. The results showed that the differences between two formulations based on the reference drug, $Soleton^{\circledR}$ were 6.33, 5.91 and 17.7% for $AUC_t$, $C_{max}$ and untransformed $T_{max}$, respectively. There were no sequence effects between two formulations in these parameters. The 90% confidence intervals using logarithmically transformed data were within the acceptance range of log 0.8 to log 1.25 (e.g.,log $1.01{\sim}1og\;1.11$ and log $0.928{\sim}1og\;1.18$ for $AUC_t$ and $C_{max}$, respectively). Thus, the criteria of the KFDA bioequivalence guideline were satisfied, indicating SCD Zaltoprofen tablet was bioequivalent to $Soleton^{\circledR}$ tablet.

Research on the Effect of the Control Methods of Irrigation Water on the Growth and Yield of Paddy Rice. (한발기에 있어서 용수관리 방법이 수도생육과 그 수량에 미치는 영향에 관한 연구)

  • 김시원
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.13 no.1
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    • pp.2177-2190
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    • 1971
  • This experiment was made to determienthe effect of various soil moisture contents in simulated drought conditions on different stages of rice growth. The drought conditions were developed at such three rice-growing stages as transplanting, immediately after transplanting and young ear forming. Three different lengths of drought periods, which are ten days, twenty days and thirty days, were applied for each growing stage of rice. The rice variety used this experiment is Nong-rim 29. This experiment was conducted at the university farm of the Kon-Kuk University during the period of $1968{\sim}1970$. Three reprications for each of 12 treatments and split plot design were employed in this study. Bottomless wood square boxes, $1^m{\times}1^m{\times}1^m$, were burried in the test plot and box top was covered with poloyethylene sheets to avoid natural rainfall drops. Standard plots were irrigated continuously with a water depth of 40mm/day and those of drought treatments were irrigated continuously up to the beginning of the planned drought period, and they were irrigated again with a depth of 40mm/day up to the maturing stage of rice. Other methods for rice raising followed those methods developed by the Field Crops Experiment Station of the Office of Rural Development. During this experiments, climatic conditions in regard to rainfalls, sunshine hours, and temperatures were observed. According to this observation, those values measured deviate slightly from the annual means. However the growing condition of rice plants were normal. The pH value of irritation water is nearly neutral, and soils in the test plots are relatively fertile, being similar to ordinary paddy soils. Analysis of variances for number of stalks, plan-height, ear sprouting date, length of stalks, ear length, number of ears per plant, fertility, grain weitght, weight of plant, and yield were carried out. The variances for plant height, ear sprouting date, length of stalk ear length, and yield has statistical significance under drought treatments applied at three different growing stages. The variance showing the effect of lengths of drought period is highly significant for all the treatments studied except that of grain weight. The interaction between drought periods and drought treatments at different growing stages is significant for plant height, stalk length, ear length, number of ears, fertility and yield, these results indicated that droughts at different growing stages have influence on plant height, ear length, yield, and length of drought period also has strong influence on all factors studied except grain weight. The combination of drought treatments at different rice growing stages and lengths of drought periods has different effects on various agronomic characteristics, including yield. Plant height under drought treatment practiced at transplanting stage is the lowest, and drought treatment applied immediately after transplanting resulted in the least number of stalks. The effect of different lengths of drought periods on plant height and number of stalks depends signis ficantly on increasing days of drought. Ear sprouting date tends to be delayed for one or two days undedrought treatments at transplanting period and with increasing days of drought. Better yield is secured in drought treatment applied immediately after transplanting. Adverse effect war observed when drought treatment was applied at ear forming period. These effects may be attributed to the alternation of irrigation and drought causing vigorous root activity. In general, yield linearly decreases as the length of the drought period increases. The results obtained in this study demonstrate that, in order to mimimize damage due to drought, and, to save irrigation water, paddy fields, immediately after transplanting, may be not irrigated, since there is sufficient moisture in the soil, and that sufficient irrigation water should be applied again in the ear forming stage of rice plant.

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Fatty Acid Compositions and Physicochemical Properties of Feta Cheese Made from Bovine Milk (우유로 제조한 휘타치즈의 지방산 조성과 물리화학적 특성)

  • 박승용
    • Journal of Animal Science and Technology
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    • v.48 no.4
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    • pp.611-622
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    • 2006
  • Fatty acid compositions and physicochemical properties of feta cheese made from bovine milk were studied. Nutritional compositions of feta cheese were fat 22.79%, protein 10.57% with moisture content of 59.87%. The log cfu/g of lactic acid bacteria in bovine feta cheese decreased from 10.25 to 7.95 and pH also changed from pH 6.22 to pH 5.55 during storage at 4℃ for 14 d aging. The color of feta cheese turned into more whitish (L-value, 100.1) with a red (a-value, 4.6) and gray (b-value,-4.1) color after 14day's aging. For the texture profile analysis of bovine feta cheese, resilience was increased significantly (p<0.01) throughout the aging periods and adhesiveness was rapidly increased right after progressing of aging at both temperatures, but no difference was found between the aging periods. Hardness, fracturability, gumminess and chewiness were gradually increased at 0℃, but no statistical significances were found. Springiness and cohesiveness were not changed at both temperatures. In organoleptic evaluations, organoleptic intensities in sweetness, milky taste and saltiness were significantly enhanced over those of the control cheese at the level of p<0.01, and masticatory texture at p<0.05 with the progress of aging to 14d. Organoleptic preferences were significantly (p<0.01) enhanced except smell, color, mouth feel, and masticatory texture with the aging. In the fatty acid compositions of feta cheese analyzed by gas chromatography, the content of SFA (52.61%) was slight higher than that of USFA (47.39%) composed with MUFA (28.98%) and PUFA (18.41%). Among the nutritionally important fatty acids; ω6 (9.27%) and ω3 (0.55%) fatty acids, CLA (0.12%), arachidonic acid (0.19%) and DHA (0.12%) were also found in bovine feta cheese.

The Concepts of Weight Control and Dietary Behavior in High School Seniors (고등학교 3학년생의 체중조절에 대한 인식과 관련 식행동 분석)

  • Chung, Hye-Kyung;Lee, Hae-Young
    • Journal of Nutrition and Health
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    • v.43 no.6
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    • pp.607-619
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    • 2010
  • The purpose of this study was to identify the concepts of body image, weight control and food behavior in 520 Korean high school seniors from Seoul and Gyeonggi Province. A questionnaire survey was conducted to investigate body image, concern of weight control, experience of weight control, the first time to try weight control, plan for weight control, reasons of weight control and food behaviors related with weight control. Anthropometric parameters such as weight and height were measured. Finally, data from 497 high school seniors were used for statistical analysis. The results were as follows. Many subjects had misperceptions. Among the subjects who perceived themselves as 'fat', 57.8% were normal weight and 0.5% underweight. Among the subjects who perceived themselves as 'thin', 59.5% were normal weight, 0.9% overweight and 0.9% obese. Girls were more concerned about weight control than boys (4.20 vs. 3.66). Weight loss had been experienced by 61.5% of the subjects and weight gain by 12.8%. First weight control was attempted by 71.3% of the subject at high school and by 28.7% at middle school. The majority of subjects planned for weight control before their freshman year. The boys choose 'health' as the primary reason for weight control, whereas the girls chose 'good appearance'. The total score of food behaviors varied significantly according to BMI. Overweight and obese subjects had more desirable food behaviors than normal weight and underweight subjects (24.00, 24.06 vs. 21.92, 21.52). However, total scores of food behaviors showed no variation according to sex, weight control attempt and body image. In conclusion, the high school seniors had misconceptions about body image and weight control. They were exposed to a high risk of inappropriate weight control and food behaviors. Therefore, proper nutritional education programs need to be developed for high school seniors to prevent nutrition problems related to weight control and to maintain desirable food behaviors.

Effectiveness of Soft Stimulation Protocol, Compared with Conventional GnRH Antagonist Multiple dose Protocol in Patients Undergoing Controlled Ovarian Stimulation with Intrauterine Insemination (과배란유도하 자궁강내 인공수정시술을 받는 환자에서 연성자극요법과 성선자극호르몬 길항제 다회투여법의 효과 비교)

  • Kim, Chung-Hoon;Kang, Hyuk-Jae;Kim, So-Ra;Jeon, Gyun-Ho;Lee, Hyang-Ah;Kim, Sung-Hoon;Chae, Hee-Dong;Kang, Byung-Moon
    • Clinical and Experimental Reproductive Medicine
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    • v.37 no.2
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    • pp.135-142
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    • 2010
  • Objective: To evaluate the effectiveness of soft stimulation protocol using GnRH antagonist/clomiphene citrate (CC)/recombinant FSH (rFSH) in patients undergoing controlled ovarian stimulation (COS) with intrauterine insemination (IUI), compared with GnRH antagonist multiple dose protocol (MDP) using GnRH antagonist/rFSH. Methods: Eighty infertile women were randomized to soft stimulation protocol group (n=40) or GnRH antagonist MDP group (n=40). In both groups, IUI was performed 36~40 hours after hCG injection. Statistical analysis was performed using Student's t-test, $\chi^2$ test or Fisher's exact test as appropriate. Results: Total dose and days of rFSH required for COS were significantly fewer in soft stimulation protocol group (p<0.001, p<0.001). A premature LH surge did not occur in any patients of both groups. Clinical pregnancy rate per cycle was similar between the two groups. Conclusion: Soft stimulation protocol provides comparable pregnancy rates to GnRH antagonist MDP despite fewer total dose and days of rFSH, and so can become one of the patient-friendly, cost-effective alternatives for infertile patients undergoing COS with IUI.

A Survey on the Knowledge and Attitudes of Women in Taegu toward Weaning Diet for Infants (이유식에 대한 대구지역 여성들의 인식조사)

  • Kim, Kyung-Ah;Lee, Eun-Sil;Lee, Young-Hwan;Shin, Son-Moon;Choi, Kwang-Hae
    • Journal of Yeungnam Medical Science
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    • v.14 no.2
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    • pp.370-382
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    • 1997
  • Successful weaning is very important to provide adequate nutrients for normal growth and to induce a good diet habit. We conducted a survey on the knowledge and attitudes of women who live in Taegu toward weaning in infants, to figure out how much they know about weaning diet for the infants, which source they prefer to get the information about weaning diet, whether their knowledge affect their practice about weaning in infants, and the most effective way to educate them. A survey was conducted with the prepared questionnaire on women who responded at the residential area and market place, and those who visited Department of Pediatrics, Yeungnam University Hospital from July 25 to September 15, 1997. Statistical analysis was done with student t-test and ANOVA using SPSS program. Among total 303 responders, those who aged between 30 and 39 years were 37.2%. Scores for the six questions about weaning policy showed highest in the age group between 30 and 39 years. Higher score was recorded in the more educated group, in those who get informations about weaning diet from books, magazines and pediatricians. The majority of the responders had wrong concepts, such as feeding weaning diet using bottle(69.1%), feeding mixed with formula in the same bottle(64.5%), addition of salt(68.4%). Those who had higher educational level preferred books, magazines rather than neighbors or mother to get informations about weaning diet. Those who preferred books or magazines started weaning, stopped bottle feeding, and used spoon earlier, and their children had unbalanced diet habits less frequently. Home-made food was used most frequently, and commercial products were used less frequently. Those who were employed preferred home-made food rather than commercial products, it may be caused by the difference of the educational backgrounds from those of the unemployed. In conclusion, most responders ask neighbors and mother to get informations about weaning, and the incorrect knowledge from them could cause inadequate weaning practice and induce bad diet habit in their children. We have to find the reasons why they did not refer books or magazines frequently, and the way to resolve the problem.

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Clinical Study of the Correlation of Tumor Necrosis Factor Alpha and the Proteinuria of Henoch-Schönlein Nephritis and Idiopathic Nephrotic Syndrome (Henoch-Schönlein Purpura 신염과 특발성 신증후군에서 Tumor Necrosis Factor Alpha와 단백뇨와의 관련성에 관한 임상적 연구)

  • Jeong, Dong-Ho;Park, Jeong-Hyun;Jeong, Hye-Cheon;Koo, Hyun-Hoe;Lee, Jun-Ho;Ha, Tae-Sun
    • Clinical and Experimental Pediatrics
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    • v.45 no.2
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    • pp.240-246
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    • 2002
  • Purpose : It is not clear that the development of glomerular injury and aggravation by tumor necrosis factor alpha($TNF-{\alpha}$) is related to intrarenal or serum concentration of $TNF-{\alpha}$. So, we studied the relationship between the concentration of $TNF-{\alpha}$ and aggravation of glomerular damage in the Henoch-$Sch{\ddot{o}}nlein$ nephritis(HSN) and idiopathic nephrotic syndrome(INS). Methods : We collected the sera and urines of 21 patients with Henoch-$Sch{\ddot{o}}nlein$ purpura(HSP) and 22 patients with INS visited Chungbuk National University hospital from March 1998 to March 2001. The concentration of $TNF-{\alpha}$ in the sera and urines were measured by sandwich ELISA. Results : Serum $TNF-{\alpha}$ levels in the HSP patients with renal involvement were significantly higher than those without renal involvement(P=0.009). But urine $TNF-{\alpha}$ levels have no correlation with renal involvement(P=0.088). In the HSN patients, proteinuria have a significant correlation with serum $TNF-{\alpha}$ levels(P=0.004) but less correlation with urine $TNF-{\alpha}$ levels(P=0.053). Otherwise, proteinuria have no correlation with serum $TNF-{\alpha}$ levels(P=0.763) but have a significant correlation with urine $TNF-{\alpha}$ levels(P=0.007) in INS. Conclusion : These result suggest that the serum concentration of $TNF-{\alpha}$ would be important to glomerular involvement in HSP. And, it is interesting that proteinuria shows a significant relation with serum $TNF-{\alpha}$ levels in the HSN, but with urine $TNF-{\alpha}$ levels in the INS. This means the major production of $TNF-{\alpha}$ may be originated by extrarenal inflammation in the HSN and by intrarenal tubulo-interstitial damage due to proteinuria in the INS.