• Title/Summary/Keyword: statistical analysis reproducibility

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Exploratory Data Analysis for microarray experiments with replicates

  • Lee, Eun-Kyung;Yi, Sung-Gon;Park, Tae-Sung
    • Proceedings of the Korean Statistical Society Conference
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    • 2005.11a
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    • pp.37-41
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    • 2005
  • Exploratory data analysis(EDA) is the initial stage of data analysis and provides a useful overview about the whole microarray experiment. If the experiments are replicated, the analyst should check the quality and reliability of microarray data within same experimental condition before the deeper statistical analysis. We shows EDA method focusing on the quality and reproducibility for replicates.

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Gauge Capability Analysis and Designed Experiments (계측기 능력분석과 실험계획법)

  • Baik, Jaiwook;Jo, Jinnam
    • Journal of Korean Society for Quality Management
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    • v.24 no.3
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    • pp.145-159
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    • 1996
  • In today's organization, measurement plays a crucial role in helping improve process or quality. In this paper, we review the measurement error study, classical gauge repeatability and reproducibility study, and designed experiment suited for the determination of the measurement capability. Measurement error study is very simple to use but is rather crude. Hence, it should be used as a preliminary study to determine whether further study is necessary. Classical gauge repeatability and reproducibility (GR&R) study is the most common technique for evaluation of gauge capability. It calculates a percentage that relates the repeatability, reproducibility, and overall R&R to the specification range for the parameter measured. Hence, the individual repeatability and reproducibility statistics will indicate the area on which to concentrate. However, GR&R study only gives a point estimate of each component, which leaves a room for improvement. It is always good to integrate the use of control charts to ascertain the statistical stability of the measurement process. The tools of statistical experimental design are available for a comprehensive design and analysis of the measurement process. Hence, in this paper, we present gauge capability analysis as an experimental design problem and compare it with the classical GR&R study.

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A study on the reproducibility of the natural head position according to the skeletal malocclusion types and sex (부정교합의 유형과 성별에 따른 자연두부위치의 재현성에 관한 연구)

  • Kim, Ha-Ran;Lee, Dong-Yul;Kim, Kwang-Won;Yoon, Young-Jooh
    • The korean journal of orthodontics
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    • v.30 no.3 s.80
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    • pp.307-315
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    • 2000
  • This study was performed to evaluate the reproducibility of natural head position according to skeletal malocclusion types and sex using cephalometric radiographs for establishing orthodontic diagnosis and treatment planning. The sample consisted of 90 young adults (male 45, female 45) who had the skeletal malocclusion. Cephalometric radiographs were taken in natural head position, and statistical analysis was performed and method error of 6 postural variables were estimated to evaluate the reproducibility of the natural head position. The following results were obtained : 1. In the reproducibility of the natural head position, postural variables had no statistical significance In male and female (P>0.05). 2. In the reproducibility of the natural head position, postural variables had no statistical significance in Class I, II, III, and total group (P>0.05). 3. The reproducibility of natural head position using method error was excellent in all groups.

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Development of a Reproducibility Index for cDNA Microarray Experiments

  • Kim, Byung-Soo;Rha, Sun-Young
    • Proceedings of the Korean Statistical Society Conference
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    • 2002.05a
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    • pp.79-83
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    • 2002
  • Since its introduction in 1995 by Schena et al. cDNA microarrays have been established as a potential tool for high-throughput analysis which allows the global monitoring of expression levels for thousands of genes simultaneously. One of the characteristics of the cDNA microarray data is that there is inherent noise even after the removal of systematic effects in the experiment. Therefore, replication is crucial to the microarray experiment. The assessment of reproducibility among replicates, however, has drawn little attention. Reproducibility may be assessed with several different endpoints along the process of data reduction of the microarray data. We define the reproducibility to be the degree with which replicate arrays duplicate each other. The aim of this note is to develop a novel measure of reproducibility among replicates in the cDNA microarray experiment based on the unprocessed data. Suppose we have p genes and n replicates in a microarray experiment. We first develop a measure of reproducibility between two replicates and generalize this concept for a measure of reproducibility of one replicate against the remaining n-1 replicates. We used the rank of the outcome variable and employed the concept of a measure of tracking in the blood pressure literature. We applied the reproducibility measure to two sets of microarray experiments in which one experiment was performed in a more homogeneous environment, resulting in validation of this novel method. The operational interpretation of this measure is clearer than Pearson's correlation coefficient which might be used as a crude measure of reproducibility of two replicates.

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Quantitive Evaluation of Reproducibility of Embankment for Full Scale Test through Statistical Analysis of Physical Properties of Soil (지반물성치 통계분석을 통한 실규모 시험용 제방축조의 재현성에 관한 정량적 평가)

  • Lee, Heemin;Moon, Junho;Kim, Minjin;Kim, Younguk
    • Journal of the Korean GEO-environmental Society
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    • v.23 no.6
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    • pp.19-23
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    • 2022
  • For the substantiation and verification of studies related to the construction of a levee using riverbed soil, real-scale levee construction and experimental studies are essential. One of the most important factors in the experimental study is the reproducibility of the multiple levees with the same initial conditions. Quantitative analysis of the reproducibility should be presented. In this study, a number of physical properties (specific gravity test, sieving test, liquid-plastic limit test, compaction test, on-site Density test) for multiple embankments built with fine-grained bed soil was obtained. The collected data then used to obtain the possibility of reproducing levee through statistical analysis to suggest a process of indicating a numeric initial condition of the real-scale test. As a result of statistical analysis to verify the aforementioned process, it was confirmed that it was possible to quantitatively evaluate the reproducibility of the construction under the same conditions of embankments. This is expected to be a basic data for a full-scale embankment test using riverbed soil including other soil based real-scale tests.

Analysis of Reproducibility of Constitutions dy IGM(InyoungGigu Macjin:人迎氣口脈法) and Agreement Rate between Sasang Constitutions dy QSCCII and Constitutions by IGM (인영기구맥법을 이용한 한의사 A씨의 체질감별 재현성 및 QSCCII체질과의 일치율 분석)

  • Park Soo-Jin;Kwon Young-Kyu
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.20 no.1
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    • pp.262-267
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    • 2006
  • We verified the reproducibility of constitution by IGM and the agreement rate between sasang constitution by QSCCII and constitution by IGM. We analyzed the results of 64 subject who were judged as identical sasang constitution by repeated QSCC II. First, we compared the two results of constitutions by IGM hat peformed by one O.M.D(Oriental Medicine Doctor) at an interval of one year. Second, we examined hypothesis established by one O.M.D, that is about relation of constitutions by IGM and QSCCII, such as I type-Taeeumin, II type-Soyangin and III type-Soeumin in constitutions. There were no statistical significances in reproducibility of constitution by IGM. Although there was a tendency of relevance of sasang constitution by QSCC II with constitution by IGM, there was no statistical significance. Hypothesis that is established by clinical experiences must be verified by an effective statistical method. And there is a necessity to complement the method including mechanical measurements about constitution classification which are performed by a diagnosis relying on individual intuition.

A Brief Guide to Statistical Analysis and Presentation for the Plant Pathology Journal

  • Jeon, Junhyun
    • The Plant Pathology Journal
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    • v.38 no.3
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    • pp.175-181
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    • 2022
  • Statistical analysis of data is an integral part of research projects in all scientific disciplines including the plant pathology. Appropriate design, application and interpretation of statistical analysis are also, therefore, at the center of publishing and properly evaluating studies in plant pathology. A survey of research works published in the Plant Pathology Journal, however, cast doubt on high standard of statistical analysis required for scientific rigor and reproducibility in the journal. Here I first describe, based on the survey of published works, what mistakes are commonly made and what components are often lacking during statistical analysis and interpretation of its results. Next, I provide possible remedies and suggestions to help guide researchers in preparing manuscript and reviewers in evaluating manuscripts submitted to the Plant Pathology Journal. This is not aiming at delineating technical and practical details of particular statistical methods or approaches.

Three-dimensional evaluation on the repeatability and reproducibility of dental scanner-based digital models (치과용 스캐너로 채득한 디지털 모형의 반복성 및 재현성에 관한 3차원적 평가)

  • Lee, Gyeong-Tak;Kim, Jae-Hong;Kim, Woong-Chul;Kim, Ji-Hwan
    • Journal of Technologic Dentistry
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    • v.34 no.3
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    • pp.213-220
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    • 2012
  • Purpose: The aim of this study was to determine the repeatability and reproducibility of two dental scanners. Methods: The master die and the stone replicas(Kavo, Germany) were digitized in touch-probe scanner(Incise, Renishaw, UK), white light scanner(Identica, Medit, Korea) to create 3-dimensional surface-models. The number of points in the point clouds from each reading were calculated and used as the CAD reference model(CRM). Discrepancies between the points in the 3-dimensional surface models and the corresponding CRM were measured by a matching-software(Power-Inspect R2, Delcam Plc, UK). The t-student test for one samples were used for statistical analysis. Results: The reproducibility of both scanner was within $3{\mu}m$, based on mean value. The mean value between measurements made directly on the touch probe scanner digital models and those made on the white light scanner digital models was $2.20-2.90{\mu}m$, and was statistically significant(P<0.05). Conclusion: With respect to adequate data acquisition, the reproducibility of dental scanner differs. Three-dimensional analysis can be applied to differential quality analysis of the manufacturing process as well as to evaluation of different analysis methods.

Uncertainty in Determination of Menthol from Mentholated Cigarette (담배 중 멘톨 분석에 대한 불확도 측정)

  • 장기철;이운철;백순옥;한상빈
    • Journal of the Korean Society of Tobacco Science
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    • v.22 no.1
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    • pp.91-98
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    • 2000
  • This study was carried out to evaluate the uncertainty in the analysis of menthol content from the mentholated cigarette. Menthol in the sample cigarette was extracted with methanol containing an anethole as an internal standard, and then analyzed by gas chromatography. As the sources of uncertainty associated with the analysis of menthol, were the following points tested, such as the weighing of sample, the preparation of extracting solution, the pipetting of extracting solution into the sample, the preparation of standard solution, the precision of GC injections for standard & sample solution, the GC response factor of standard solution, the reproducibility of menthol analysis, and the determination of water content in tobacco, etc. For calculating the uncertainties, type A of uncertainty was evaluated by the statistical analysis of a series of observation, and type B by the information based on supplier's catalogue and/or certificated of calibration. Sources of uncertainty were subsequently included and mathematically combined with the uncertainty arising from the assessment of accuracy to provide the overall uncertainty. It was shown that the main source of uncertainty came from the errors in the reproducibility of menthol and water determination, the purity of menthol reference material in the preparation of standard solution, and the precision of GC injections for sample solution. The errors in sample weighing and volume measurement contributed relatively little to the overall uncertainty. The expanded uncertainty in the mentholated cigarettes, Korean and American brand, at 0.95 level of statistical confidence was $\pm$0.06 and $\pm$0.07 mg/g for a menthol content of 1.89 and 2.32 mg/g, respectively.

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