• 제목/요약/키워드: starch size

검색결과 309건 처리시간 0.034초

산마늘 다신초 증식과 인경형성에 효율적인 생물반응기 배양방식 (High Efficiency Bioreactor Culture System for Mass Proliferation and Bulblet Formation of Allium victorialis var. platyphyllum Makino)

  • 박소영;이위영;안진권;권영진;박혜진
    • Journal of Plant Biotechnology
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    • 제31권2호
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    • pp.127-132
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    • 2004
  • 본 실험은 산마늘의 산초 증식과 인경 형성에 적합한 생물반응기 배양방식과 인경 형성시 배양방식에 따른 당 대사를 구명하고자 실시되었다. 다신초 증식에는 생물반응기에 망을 걸고 배지에 절편체를 접하게 배양한 RC와 MRC (13-15개)에서 가장 좋은 결과를 얻었다. 인경형성과 비대에는 간헐적으로 배지를 공급해준 E&FS에서 93.4%의 인경형성이 이루어졌고 크기에 있어서도 균일하였다. RC와 MRC에서 형성된 인경은 뿌리가 무성하였으며 인경의 크기도 균일하지 않았다. 배양방식별로 수확한 인경내 유리당 함량은 전반적으로 E&FS.에서 낮았던데 반해 전분 함량은 높았다. 배지내 sucrose, glucose와 fructose는 인경 비대 시기에 감사되었는데 이는 첨가한 sucrose가 가수분해 되기도 전에 glucose나 fructose와 함께 식물체로 바로 이용됨을 보여주는 것이었다.

Source-sink Relationships of Soybean as Influenced by Drought Stress during the Pod and Seed-developing Stage

  • Shin Seong-Hyu;Park Keum-Yong;Shin Sang-Ouk;Lim Sea-Gyu;Ha Tae-Joung;Kim Do-Soon
    • 한국작물학회지
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    • 제51권4호
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    • pp.310-317
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    • 2006
  • This study was conducted to investigate the influence of drought stress during the pod developing and seed filling stage on source-sink relationships of soybean (Glycine max). Drought treatments were imposed by withholding water at the full-pod stage, 19 days after flowering, and then limited watering was relieved at 15 days after the initiation of drought treatment. Soybean seed yield was reduced by 39% mainly due to decreased pod number under drought stress, but the 100-seed weight was relatively less reduced. In spite of the 15-day drought during the full-pod stage, soybean produced good seeds showing similar l00-seed weight, protein, starch and soluble sugar content to those from the well-watered. Although drought during the full-pod stage caused source limitations; i.e. accelerated leaf senescence and reduced leaf soluble sugars, it did not cause limitations of other source characteristics such as SGR and leaf starch level. This is because the reduction in size of sinks, such as pod and seed abortions compensated for source limitations, resulting in balanced source-sink as expressed by LAR and the ratio of leaf area to seed dry weight. Drought stress during the pod developing and seed filling stage did not disrupt the source-sink balance

밀가루의 수침기간이 전분 및 배접용 풀의 특성에 미치는 영향 (Effect of the Fermentation Time of the Fermented Wheat Starch and Paste on the Properties for Pasting)

  • 백영미;조경실;이영희
    • 보존과학회지
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    • 제25권1호
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    • pp.39-47
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    • 2009
  • 본 연구는 전통적인 방법으로 배접용 밀가루 수침 풀의 수침기간에 따른 전분의 구조 및 풀의 접착 특성에 미치는 영향을 고찰한 결과이다. 본 연구는 향후 회화문화재의 보존처리용 천연접착제 개발을 위한 기초 자료로 제시하고자 한다. 따라서 본 연구에서는 고급표구(장황)에 사용하는 밀가루 풀의 수침기간이 2년8개월, 4년8개월, 7년인 세 가지 시료를 준비하여 총당함량, 아밀로오스 함량, 결정화도, 입자의 형태 및 크기, 호화특성, 점도, pH, 박리강도 등을 측정하였다. 그 결과 수침기간이 길어질수록 총당함량, 결정화정도, 최고점도, 최저점도, 최종점도, 강하점도, 및 치반점도는 증가하는 경향을 나타내었고 아밀로오스 함량, 호화온도, pH는 감소하는 경향을 나타내었다. 접착강도는 줄어드는 경향이 있었으나 7년 삭힌 시료의 경우 오히려 박리강도가 증가하였다.

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벼품종 ″Pokhareli Mashino′에서 유래된 Opaque형질의 배유특성 (Characteristics of Opaque Endosperm Originated from a Rice Cultivar ′Pokhareli Mashino′)

  • 김용권;허문회
    • 한국작물학회지
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    • 제34권2호
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    • pp.155-162
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    • 1989
  • 수도 품질다양화를 위한 품종육종에 이용하고자 India 품종 Pokhareli Mashino에서 발견된 저 amlose opaque형질에 대한 배유의 이화학적 특성을 조사하였다. 얻어진 결과를 요약하면 다음과 같다. 1. Opaque 특성을 가진 계통 CB 243은 쌀의 외관적 특성은 찰벼와 같았으나 요드용액에 대한 반응은 자청색으로 메벼와 같았다. 2. Opaque쌀의 전분형태는 찰·메벼와 차이가 없이 다각형입자이었으나 전분입의 크기는 타 품종에 비하여 현저히 작았다. 3. Opaque 쌀 전분입의 결정구조는 A형을 나타냈다. 4. Opaque쌀의 총 단백질함량은 13.06%로서 다른 품종들에 비해 약 50%정도 높았으나 아미노산 조성비율은 차이가 없었다. 5. Opaque쌀의 알카리 붕괴도는 1.7% KOH 용액에서 1.8정도로 다른 품종에 비해 매우 낮았다. 6. Opaque쌀의 전체 수분흡수량과 흡수속도는 찰과 고 amylose계통에 비하여 적고 늦었으며 메벼 통일과 비슷하였다. 7. Opaque쌀의 amylogram 특성은 호화 개시시간이 36분, 최고점도 980BU,최저점도 500BU, Steback - 260BU, Consistency 200BU로 찰과 메의 중간값을 보였다.

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Xylanase를 생산하는 호알칼리성 균주의 분리 및 동정 (Isolation and Identification of Alkalophilic Microorganism Producing Xylanase)

  • 최지휘;배동훈
    • 산업식품공학
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    • 제14권3호
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    • pp.263-270
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    • 2010
  • Xylanase를 생산하는 호알칼리성 균주를 토양으로부터 분리하여 동정하였다. 토양으로부터 분리한 호알칼리성 균주 3000여종 중 xylanase 활성이 가장 높은 균주인 strain DK-2386 균주를 선별하였다. Gram 염색과 SEM을 통한 형태학적 특성을 관찰하였으며, Methods for General and Molecular Bacteriology와 Bergey's manual of systematic bacteriology, 그리고 Biochemical tests for identification of medical bacteria에 준하여 생화학적 특성을 확인한 결과 배양액의 색은 붉은 빛을 나타내었으며, Gram양성 간균, 내생포자형성, catalase 양성, oxidase 양성, 혐기성 양성, glucose로부터 gas 형성 양성, casein 가수분해 양성, starch 가수분해 양성, CMC 가수분해 양성, xylan 가수분해 양성, nitrate 환원능 양성이며 10% NaCl 농도에서도 생육하는 것을 확인하였다. 균체의 지방산 조성 분석 결과 $C_{15:0\;ISO}$ 27.35%와 $C_{15:0\;ANTEISO}$ 31.54%, $C_{16:0}$ 9.70%로 이루어져 Bacillus속으로 동정되었으며, 16S rDNA 염기서열 분석결과 B. agaradhaerens와 100%의 유사성을 갖는 것을 확인하였다. (사)한국종균협회로부터 분양받은 Bacillus agaradhaerens 40952, B. alcalophilus와 strain DK-2386 균주사이의 생화학적 특성을 비교한 결과 B. agaradhaerens와 속과 종이 같으나 미세하게 특성이 다른 균주로 판단되어 B. agarad haerens DK-2386이라 명명하였다.

Characterization of Purple-discolored, Uppermost Leaves of Soybean; QTL Mapping, HyperspectraI Imaging, and TEM Observation

  • JaeJin Lee;Jeongsun Lee;Seongha Kwon;Heejin You;Sungwoo Lee
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2022년도 추계학술대회
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    • pp.187-187
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    • 2022
  • Purple-discoloration of the uppermost leaves has been observed in some soybean cultivars in recent years. The purpose of this study was to characterize the novel phenotypic changes between the uppermost and middle leaves via multiple approaches. First, quantitative trait loci mapping was conducted to detect loci associated with the novel phenotype using 85 recombinant inbred lines (RILs) of the 'Daepung' × PI 96983 population. 180K SNP data, a major quantitative trait locus (QTL) was identified at around 60 cM of chromosome 6, which accounts for 56% of total phenotypic variance. The genomic interval is about ~700kb, and a list of annotated genes includes the T-gene which is known to control pubescence and seed coat color and is presumed to encode flavonoid 35-hydroxylase (F3'H). Based on Hyperspectral imaging, the reflectance at 528-554 nm wavelength band was extremely reduced in the uppermost leaves compared to the middle (green leaves), which is presumed die to the accumulation of anthocyanins. In addition, purple-discolored leaf tissues were observed and compared to normal leaves using a transmission electronic microscope (TEM). Base on observations of the cell organelles, the purple-discolored uppermost leaves had many pigments formed in the epidermal cells unlike the normal middle leaves, and the cell wall thickness was twice as thick in the discolored leaves. The thickness of the thylakoid layer in the chloroplast the number of starch grains, the size of starch all decreased in the discolored leaves, while the number of plastoglobule and mitochondria increased.

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Changes in Growth and Quality of Melon (Cucumis melo L.) and in Soil Nitrogen Forms due to Organic Fertilizer Application

  • Park, Yang Ho;Seo, Beom Seok
    • 한국토양비료학회지
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    • 제45권6호
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    • pp.1009-1016
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    • 2012
  • The purpose of this study was to determine the effects of organic fertilizers on soil properties and growth and quality of melon. Organic fertilizer was applied in soil at the rate of 0, 0.5, 1, 2N according to Rural Development Administration guideline in Korea. The fertilizer had no effects on plant growth-rate parameters, including plant height, leaf number, and leaf size. There were minor effects on the fruit quality parameters such as fruit weight, fruit length, fruit width, placenta and seed weights, sugar content, and starch content. Ascorbic acid level was decreased as fertilizer level was increased. The level of nitrate in groundwater increased with increased levels of N.

Dissolution Characteristics of Biphenyl Dimethyl Dicarboxylate from Solid Dispersions with Copolyvidone

  • Moon, Jee-Hyun;Chun, In-Koo
    • 한국응용약물학회:학술대회논문집
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    • 한국응용약물학회 1997년도 춘계학술대회
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    • pp.118-118
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    • 1997
  • Solid dispersions were used to increase the dissolution rate of biphenyl dimethyl dicarboxylate (DDB) in water, with the ultimate goal of optimizing its bioavailability when incoporated into pharmaceuticals. Carriers used were Kollidon 30, Kollidon VA 64, 2-hydroxypropyl-${\beta}$-cyclodextrin (HPCD), sodium salicylate or sodium benzoate. DDB solid dispersions were prepared at drug to carrier proportions ranging from 1 : 5 to 1 : 20 (w/w) by solvent evaporation method. DDB tablets (7.5 mg) were prepared by compressing the powder mixture composed of solid dispersions, lactose, corn starch, crospovidone and magnesium stearate using a single-punch press. DDB capsules (7.5 mg) were prepared by filing the mixture into empty hard gelatin capsules (size #1). Dissolution studies of DDB from powdered solid dispersions, tablets and capsules were performed in 900 $m\ell$ of water at 100 rpm and 37$^{\circ}C$ by the paddle method. The dissolved amount was assayed by HPLC and expressed as the mean(%)of three determinations.

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인삼 엽록체의 미세구조와 Photobleaching (Ultrastructural Feature and Photobleaching of ginseng Chloroplasts)

  • 양덕조;김명원
    • Journal of Ginseng Research
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    • 제14권3호
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    • pp.416-420
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    • 1990
  • Ultrastructural and anatomical features of the leaf were studied in Panax ginseng C.A. Meyer(ginseng). The ginseng leaf poorly developed palisade tissue and the size of mesophyll cell was larger and the chloroplast density was lower than that of Glycine max (soyben). Ginseng chloroplast was filled with highly stacked grana and condensely-arrayed thylakoid, so the stroma space was hardly absorbed. However, ginseng mesophyll tissue and chloroplast array did not reduce light energy entering the mesophyll chloroplast, and the high LHCP/CP ratio of ginseng thylakoid resulted in the absorption of excess photon. It is reasonable to assume that 1O1-photogenearation by excess light energy partially resulted from the anatomical and ultrastructural characteristics of the ginseng leaf.

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Genetic Variations of Natural and Hatchery Populations of Korean Ayu (Plecoglossus altivelis) by Isozyme Markers

  • Han, Hyon-Sob;Jin, Deuk-Hee;Lee, Jong-Kwan
    • 한국양식학회지
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    • 제16권2호
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    • pp.69-75
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    • 2003
  • Genetic variability and population structure of 11 natural ayu, Plecoglossus altivelis populations and one hatchery stock were assessed by starch gel electrophoretic analysis with 10 enzyme coding loci. Three loci were polymorphic (lower than 0.95 in major allele frequency) in natural populations,2 loci in hatchery stock. The average number of alleles per locus was 1.38. Observed heterozygosities ranged from 0.0235 to 0.088 (0.055 on the average) in natural population while 0.0925 in hatchery stock. The genetic distance among natural populations measured 0.000047-0.005407 and no significant differentiation was observed among them. On the other hand, a signifcant genetic distance was found between natural populations and the hatchery stock with measuring 0.002032-0.O08605. The results in this study suggest that the hatchery stock has diverged from natural populations, and also that careful to maintain sustainable and effective population size (parents number) should be made.