• 대한수의학회지
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• 제33권3호
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• pp.387-391
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• 1993

Strains of Staphylococcus hyicus subsp. hyicus were compared and differentiated by staphylococcin sensitivity. Strains were tested by the cross-streaking method on brain heart infusion agar. From 31 staphylococcin producers, a typing set of 9 producers was selected and used to type all of the strains. All 81 strains of Staph hyicus subsp. hyicus tested were typable and were differentiated into 12 different sensitivity patterns. Strains of the same serotype from different sources could be further subdivided by their staphylococcin sensitivity patterns. Other staphylococcus species could also be typed by use of the 9 standard strains of Staph hyicus subsp. hyicus.

• 대한수의학회지
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• 제54권1호
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• pp.49-52
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• 2014

This study was conducted to compare the patterns of mastitic pathogens and the antimicrobial susceptibility of Staphylococcus (S.) aureus from conventional milking (CM) and robotic milking (RM) dairy herds. To accomplish this, the minimum inhibitory concentrations of 14 antimicrobial agents were tested against S. aureus by the microdilution method. Regardless of the milking system, S. aureus, coagulase negative staphylococcus, and Streptococcus uberis were isolated. Additionally, significant differences in the antimicrobial susceptibility of S. aureus isolates between RM and CM farms were only observed in response to tetracycline.

• Journal of the Korean Wood Science and Technology
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• 제30권3호
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• pp.26-33
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• 2002

The seven strains, Pseudomonas paucimobilis, Pseudomonas cepacia, Staphylococcus auricularis, Staphylococcus saprophyticus, Acidovorax spp., Acinetobacter calcoaceticus, and Actinobacillus capsulatus were tested with three slimicides. Most of the tested bacteria were inhibited with slimicide K (an isothiazolin based compound), even at its low concentration, except for Actinobacillus capsulatus and Staphylococcus auricularis. Both slimicides B (an organic bromine based compound) and S (aldehydes) also couldn't prevent these two strains even at their highest concentration. Five different sizes of plasmid DNA were isolated from Actinobacillus capsulatus. Staphylococcus auricularis, a gram-positive bacteria, showed the slimy substances around its cell distinctively. The results suggest that two strains, Actinobacillus capsulatus, Staphylococcus auricularis, have presumably developed a resistance to the slimicide, by plasmid DNA or slimy substance. Our findings also suggest that not only gram-negative bacteria, but also gram-positive bacteria should not be neglected

• Journal of Oral Medicine and Pain
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• 제23권4호
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• pp.343-352
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• 1998

This experiment was performed to investigate effects of zinc containing solution on the major normal flora Staphylococccus aureus, Streptococus mutans and Candida albicans and to observe the variation according to anionic change and concentration difference. Zinc chloride, zinc iodide and zinc acetate solution were added to werially diluted broth culture so that each final concentration might be 0.25%, 0.5%. 1%. After that, 100ul of each aliquot was spreaded on each selective media plate( Mannitol Salts Agar plate for Staphylococcus aureus, Mitis Salivarius Agar plate for Streptococcus mutans and Sabouraud Destrose Agar plate for Candida albicans). The % killing was calculated bu CFU count after incubation under the appropriate condition. 1. zinc iodide, zinc chloride, and zinc acetate solutions showed inhibitory effects on Staphylococcus aureus, Streptococcus mutans and Candida albicans. 2. The inhibitory effects on Staphylococcus aureus were ranked in order of ainc iodide, zinc chloride and zinc actate. 3. The inhibitory effects on Streptococcus mutans were ranked in orfer of zinc iodide, zinc chloride and zinc acetate. 4. the inhibitory effects on Candida albicans showed no difference among zinc iodide, zinc chloride and zinc acetate. 5. The inhibitory effects of zinc chloride and zinc acetate on Staphylococcus aureus and Streptococcus mutnas showed increasing pattern as the concentration increase. But the inhibitory effects of zinc iodide on Staphylococcus aureus and Streptococcus mutans showed no apparent difference according to concentrations and it was the case with the inhibitory effects of zinc iodide, zinc chloride and zinc acetate on Candida albicans.

• 한국식품위생안전성학회지
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• 제21권2호
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• pp.70-75
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• 2006

서울시 송파구 가락동에 위치한 가락 농수산물 도매 시장에서 구입한 건포류 32건(오징어류 14건, 명태류 10건, 쥐치포 4건, 대구포 그리고 조개류 각각 2건씩)을 조사한 결과를 보면, 그 평균치가 수분 17.28%, 회분 4.92%, 일반 세균수 $1.1{\times}10^7$ CFU/ml, 대장균군수 $9.0{\times}10^3$ CFU/ml, 그리고 포도상구균수 $5.4{\times}10^6$ CFU/ml로 각각 나타났다. 분리 동정된 111주를 살펴보면, 72주의 대장균군속균에서는 21주의 Pantoea spp, 14주의 Enterobacter cloacae, 그리고 13주의 Ranella aquatilis 등이 주로 나타났고, 포도상구균속균 12주 중에서는 Staphylococcus xylosus가 4주로 가장 많았다.

• 한국식품위생안전성학회지
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• 제8권3호
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• pp.141-145
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• 1993

수종의 병원성 미생물에 대한 39여종의 생약 열수추출액의 항균성을 조사한 결과 다음과 같은 결과를 얻었다. 황백 Phellodendron amurense 및 황령 Coptis chinensis 는 Staphylococcus aureus균의 생육을 억제시켰으며 복분자 Rubus creanus는 Staphylococcus aureus 및 Pseudomonas aeruginosa에 항균력을 나타내었다. 진피 Citrus unshiu는 E.coli에 대해서 산수유 Cornus officinalis는 Candida albicans의 생육을 억제시켰고 우슬 Achyranthes japonica 및 감초 Clycyrrhiza uralsis는 Aspergillus niger에 뚜렷한 항균효과를 나타내었다. 또한 감초는 Staphylococcus Aureus, E. coli, Pseudomonas aeruginosa , Aspergillus niger 모두에 항균력을 나타내었다.

• Journal of Korean Biological Nursing Science
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• 제10권2호
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• pp.147-153
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• 2008

Purpose: Methicillin Resistant Staphylococcus aureus (MRSA) has become a major clinical problem and one of the major nosocomial pathogen worldwide. The aim of the study was to investigate the epidemiological characteristics of genotypes of MRSA isolated in the A-hospital ICU. Methods: In the period between December 2007 and May 2008, MRSA was isolated from ICU patients and its surrounding environment. Polymerase Chain Reaction (PCR) was conducted for the detection of MRSA gene. The incidence of MRSA in the clinical isolates of Staphylococcus aureus was examined by using a multiplex PCR. The spa gene of Staphylococcus aureus encodes protein A and is used for typing of MRSA. We used sequence typing of the spa gene repeat region to study the epidemiology of MRSA at a hospital. Results: Two different genotypes of MRSA were identified with 90 isolated from the patients and its surrounding environments in the ICU. Conclusion: This study may contribute to the development of effective strategies for preventing nosocomial infections. Genotyping may have more general application for the study of MRSA epidemic outbreak in hospital and community infection.

• 대한수의학회지
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• 제47권1호
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• pp.43-50
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• 2007

Staphylococci are Gram-positive, facultatively anaerobic cocci, normally found on the skin andmucosal surfaces of most warm-blooded animals and often involved in a wide variety of diseases in animals.Staphylococcal infections are treated with antibiotics and, consequently, antibiotic resistance and/or acquiredresistance have developed. Staphylococcus (S.) intermedius and Staphylococcus (S.) aureus are 2 comonveterinary isolates that are frequently associated with suppurative infections. This study was undertaken toexamine antimicrobial susceptibility of S. aureus (23 isolates) and S. intermedius (160 isolates) isolatedfrom dogs in Gwangju, Korea and investigate whether the antibiotic resistance of S. aureus and S.intermedius is efected by the site of isolation, age, and sex of dogs. More isolates were isolated fromadult dogs (71.3%) than juveniles (20.5%). Antimicrobial resistance was commonly found for Penicillin,Tetracycline, Trimethoprim-Sulphamethoxazole in both Staphylococcus species. All of the isolates weresusceptible to Amoxicillin/Clavulanic Acid, Cephalothin, Oxacilin, Neomycin, and Vancomycin. Appropriateprotocol for antibiotic use and strategies to reduce antimicrobial resistance rate will be needed. Periodicsubstitution of antimicrobial agents and limitation of antibiotic use should also be considered.

• 한국동물위생학회지
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• 제35권1호
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• pp.63-67
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• 2012

An 8-months-old young Maltese bitch was presented for investigation of anorexia and occasional vomiting. The body weight was 2 kg. Abdominal sonography demonstrated abnormal enlargement of uterus. The dog had been clinically and pathologically examined. Also, ovariohystectomy was performed. Staphylococcus aureus was identified in the pus-like materials of the uterine lumen. This case was confirmed as pyometra induced by Staphylococcus aureus. Gross examination of the dissected uterus revealed enlarged uterine horn, which had yellowish or brownish pus-like materials in the lumen. Also, there was several segments formation between the enlarged areas of left and right uterine horns. Histopathologically, endometritis and necrosis were present in uterine wall. The bitch made a complete recovery following an ovariohystectomy. This case was spontaneous pyometra induced by Staphylococcus species. On our knowledge, this is a very rare report of pyometra occurrence in young dog and Staphylococcus species-induced pyometra. This study may provide a valuable data on the study of canine pyometra.

• Applied Biological Chemistry
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• 제38권6호
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• pp.577-580
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• 1995

다제내성 Staphylococcus aureus균주에 강력한 항균활성을 나타내는 CNU30122 균주를 선발하였다. 선발된 CNU30122균주의 배양액 추출물로부터 Diaion HP-20 column chromatograpy, ethylacetate 추출, silica gel 및 Sephadex LH-20 column chromatography, HPLC 등에 의해 백색분말의 순수한 화합물을 분리정제 하였다. 본 물질의 구조분석을 위하여 $^1H$ 및 $^{13}C\;NMR,\;DEPT,\;^1H-^1H\;COSY,\;^1H-^{13}C\;COSY$ 및 HMBC spectrum을 분석한 결과 본 물질은 분자량 516, 분자식 $C_{31}H_{48}O_6$의 tetracyclic triterpenoic acid 계열인 fusidic acid로 동정 되었다. 본화합물은 streptomycin, pecicillin, kanamycin, tetracycline 등의 약제에 내성균주인 Staphylococcus aureus R209 균주에 선택적으로 강한 항균활성을 나타내었다.