• Title/Summary/Keyword: standard culture methods

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Chardin's Genre Paintings of Child Education: The Enlightenment Views on Children of the French Bourgeois Class in the 18th Century (샤르댕의 아동 교육 장르화 - 18세기 프랑스 부르주아의 계몽주의적 아동관)

  • Ko, Yu-Kyoung
    • The Journal of Art Theory & Practice
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    • no.8
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    • pp.33-58
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    • 2009
  • This paper examines four genre paintings on the subject of child education by Jean-Baptiste-Sim${\'{e}}$on Chardin(1699-1779). The Governess, The Diligent Mother, Saying Grace, and The Morning Toilette garnered critical attention after they were exhibited in the Salon from 1739 to 1741. After the exhibition, the paintings were made into prints and frequently sold to members of the bourgeois class in Paris. The iconographical details of Chardin's genre paintings have, thus far, been compared to Dutch genre pictures of the seventeenth century. Further, most studies conducted on Chardin's paintings focus on formal analysis rather than the historical and social contexts. Through attempting social-contextual readings of Chardin's educational series, this paper argues that the significance of Chardin's painting series of child education lies in his representation of the ideal French bourgeois family and the standard of early childhood education in the eighteenth-century French Enlightenment period. In each of the four child education paintings, Chardin depicted a mother with children in a domestic space. Even though this theme derives from traditional Dutch genre paintings in the seventeenth century, the visual motifs, the pictorial atmosphere and the painting techniques of Chardin all project the social culture of eighteenth century France. Each painting in the child education series exemplifies respectively the attire of a French gentlemen, the social view on womanhood and the education of girls, newly established table manners, and the dressing up culture in a 'toilette' in eighteenth century France. Distinct from other educational scenes in previous genre paintings, Chardin accentuated the naive and innocent characteristics of a child and exemplified the mother's warmth toward that child in her tender facial expressions and gesturing. These kinds of expressions illustrate the newly structured standard of education in the French Enlightenment period. Whereas medieval people viewed children as immature and useless, people in the eighteenth century began to recognize children for their more positive features. They compared children to a blank piece of paper (tabula rasa), which signified children's innocence, and suggested that children possess neither good nor bad virtues. This positive perspective on children slowly transformed the pedagogical methods. Teaching manuals instructed governesses and mothers to respect each child's personality rather than be strict and harsh to them. Children were also allotted more playtimes, which explains the display of various toys in the backgrounds of Chardin's series of four paintings. Concurrently, the interior, where this exemplary education was executed, alludes to the virtue of the bourgeois's moderate and thrifty daily life in eighteenth century France. While other contemporary painters preferred to depict the extravagant living space of a French bourgeoisie, Chardin portrayed a rather modest and cozy home interior. In contrast to the highly decorated living space of aristocrats, he presented the realistic, humble domestic space of a bourgeois, filled with modern household objects. In addition, the mother is exceptionally clad in working clothes instead of fashionable dresses of the moment. Fit to take care of household affairs and children, the mother represents the ideal virtues of a bourgeois family. It can be concluded that the four genre paintings of child education by Chardin articulate the new standards of juvenile education in eighteenth century France as well as the highly recognized social virtues between French bourgeois families. Thus, Chardin's series of child education would have functioned as a demonstration of the ideal living standards of the bourgeois class and their emphasis on early childhood education in the French Enlightenment period.

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Comparison of Noise Power Spectrum Methodologies in Measurements by Using Various Electronic Portal Imaging Devices in Radiation Therapy (방사선치료시 전자포털영상장치를 이용한 잡음전력스펙트럼 방법론 측정비교)

  • Son, Soon-Yong;Choi, Kwan-Woo;Jeong, Hoi-Woun;Kwon, Kyung-Tae;Kim, Ki-Won;Lee, Young-Ah;Son, Jin-Hyun;Min, Jung-Whan
    • Journal of radiological science and technology
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    • v.39 no.1
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    • pp.99-105
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    • 2016
  • The noise power spectrum (NPS) is one of the most general methods for measuring the noise amplitude and the quality of an image acquired from a uniform radiation field. The purpose of this study was to compare different NPS methodologies by using megavoltage X-ray energies. The NPS evaluation methods in diagnostic radiation were applied to therapy using the International Electro-technical Commission standard (IEC 62220-1). Various radiation therapy (RT) devices such as TrueBeam$^{TM}$(Varian), BEAMVIEW$^{PLUS}$(Siemens), iViewGT(Elekta) and Clinac$^R$ iX (Varian) were used. In order to measure the region of interest (ROI) of the NPS, we used the following four factors: the overlapping impact, the non-overlapping impact, the flatness and penumbra. As for NPS results, iViewGT(Elekta) had the higher amplitude of noise, compared to BEAMVIEW$^{PLUS}$ (Siemens), TrueBeam$^{TM}$(Varian) flattening filter, Clinac$^{R}$iXaS1000(Varian) and TrueBeam$^{TM}$(Varian) flattening filter free. The present study revealed that various factors could be employed to produce megavoltage imaging (MVI) of the NPS and as a baseline standard for NPS methodologies control in MVI.

A Study on Sexual Violence Consciousness and Cognition of Sexual Violence in University Students (대학생의 성폭력 인식 및 의식에 관한 기초조사)

  • Kim, Hui-Yeong;Na, Se-Hee;Nam, Kyoung-Min;Nam, Sun-Hyo;Noh, Ju-Hee;Doo, Song-Ee;Moon, Ji-Ye;Kim, Eun-Hee;Kim, Jin-A;Ahn, Seong-Ah
    • Journal of Korean Clinical Health Science
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    • v.1 no.1
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    • pp.63-74
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    • 2013
  • Purpose. The purpose of this study was to contribute to the development of the sexual education program by examining status of Sexual Violence Consciousness and Cognition of Sexual Violence of University students Methods. The subjects were 240 University students located in J-city. Data were collected during the period from Nov. 12, 2012 to Nov. 13, 2012. For the data analysis, SPSS 12.0 K program was utilized to get frequency number, percentage, average, standard deviation and t-test. Results. The results present different degrees of man and women's cause of sexual violence cognition and consciousness were as following ; The several scores of women higher more than man parts were 'Think of the woman as a sexual target(t=3.86, p<0.001)','Men's sexual urges(t=3.98, p<0.001)', 'The low status of women in society (t=3.85, p<0.001)', 'Male-dominated society and culture(t=5.62, p<0.001)', 'Acceptance of men aggression and activism(t=5.19, p<0.001)','Weak punishment (t=7.25, p<0.001)'and Man higher more than women parts were 'overexposure of women(t=3.88, p<0.001)'. Conclusion. Results suggest that sexual education program should strategies to increase a positive Consciousness and Cognition of Sexual Violence in their education programs in order to improve Consciousness and Cognition of Sexual Violence in University students.

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Functional Properties of Yogurt Fermented by Bacteriocin-producing Pediococcus acidilactici (박테리오신 생성 Pediococcus acidilactici 를 적용한 요거트 특성 및 항균성 연구)

  • Hyun, In Kyung;Kim, Min Young;Kim, Seo-Yeon;Lee, Jee-Su;Choi, Ah-Rang;Kang, Seok-Seong
    • Journal of Dairy Science and Biotechnology
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    • v.38 no.3
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    • pp.154-160
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    • 2020
  • Physical and sensory characteristics of commercial yogurts are important aspects for consumer acceptability. In addition, beneficial functions of commercial yogurts are also emphasized for the probiotic dairy products. The aim of this study was to investigate the functional properties of yogurts with the combination of bacteriocin-producing Pediococcus acidilactici. Yogurts fermented with commercial starter culture (control yogurt) and control yogurt together with P. acidilactici HW01 (yogurt+HW01), P. acidilactici JM01 (yogurt+JM01), or P. acidilactici K10 (yogurt+K10) were prepared. During 28 days after fermentation, the viability of lactic acid bacteria, pH, and brix, in the yogurt samples were assessed with standard methods. Moreover, to investigate the antilisterial activity of the yogurt samples, Listeria monocytogenes was simultaneously inoculated when the yogurts were prepared with lactic acid bacteria, and the viability of L. monocytogenes was determined. Although yogurt+K10 did not completely remove L. monocytogenes, control yogurt, yogurt+HW01, and yogurt+JM01 eradicated L. monocytogenes at day 2 after fermentation. However, yogurt+K10 also removed L. monocytogenes at day 3 after fermentation. Taken together, these findings suggest that the combination of yogurt with P. acidilactici does not affect its quality and they may consequently aid in the development of new probiotic yogurt.

A Study on the Five Colors Appearing in the Traditional Korean Bojaki of the Era of Chosun Dynasty (조선시대 보자기에 나타나는 오방색에 관한 고찰)

  • Noh Eun-Hee
    • Journal of Science of Art and Design
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    • v.6
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    • pp.81-99
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    • 2004
  • For making something to use as a tool since the emergence of mankind, the initial pragmatic purpose has transformed into a new genre of art over time. Things defined like this have rooted in our culture as tradition so far. In the midst of today's various trends, a reflection of tradition and a modern search for tradition by re-creating it are much more needed. To any people in any time, a new trend would undeniably develop on the basis of its previous tradition in any form. The colors appearing in such artwork are also an expression of each people's unconscious potentiality as essential grounds for human aesthetic. The traditional Korean Bojaki, which was made out of women's pragmatic mind trying to recycle trashy pieces of cloth in the 19th century of Chosun Dynasty, appears as a symbolization of our nation's original form in unconsciousness. It includes Confucianism, Buddhism, Zen and the Yin-Yang and Five Elements thought, which have been together with naturalism. The five colors appearing in the Yin-Yang and Five Elements are the basis. Fourteen selected samples around the five colors seen in the color scheme of the Chosun era's Bojaki were measured and their color values were found by analyzing them based on HCV(Hue, Chroma, Value)of the five primary colors as well as the five secondary colors. After choosing a few colors with bare eyes close to traditional Primary Colors and Secondary Colors amongst 14 pieces of data which particularly used traditional Five Colors and examining them using spectrophotometer(JX777), the following conclusions were drawn. Comparing only colors in Primary Colors, the result was red 7.11R 4.59/10.69, blue 6.71PB 3.18/6.45, yellow 3.91Y 7.56/6.12, respectively. With regard to Secondary Colors, it was reported that red 7.96RP 5.42/10.3, blue 7.8B 5.16/5.53, green 8.03GY 6.05/4.34, yellow 2.73Y 7.47/4.07, purple 2.39RP 4.69/4.56, respectively. (diagram) As a result, the standard of Five Colors can be used in modern fiber color. There are differences in dyeing material, methods and kinds of fiber of that time, but women of Chosun Dynasty combined and made fabric which was circulated. Consequently, an applicable attribute of the aye-color values was considered.

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Effect of Different Exercise Intensity on Blood Melatonin Density in Sleep Disordered Rats (운동 강도가 수면장애 모델 쥐의 혈 중 멜라토닌 농도에 미치는 영향)

  • Kim, Hee-Jung;Kim, Dong-Hyun
    • Journal of the Korean Society of Physical Medicine
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    • v.9 no.1
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    • pp.45-53
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    • 2014
  • PURPOSE: In this study, we tried to find out what kind of exercise was more effective in sleep disorder by comparing melatonin in blood after applying low intensity with high intensity exercise to sleep disordered rats induced by experiment. METHODS: We used male Sprague-Dawley rats which were 8weeks old and weighted 300g. They were supplied with water and food without any restriction. We kept the room temperature at $25^{\circ}C$ and controld the length of day and night in 12 hours blocks, respectively. We divided the rats 60 into 2 groups. To one group we applied low intensity exercise, and to the other we applied high intensity exercise for 15minutes per day over a period of 4 weeks. We extracted the blood from abdominal aorta before, after exercise, moved into EDTA tube, performed centrifugation. We decanted the serum $200{\mu}l$ from the blood into microcentrifuge tube by samples and moved into polypropylene culture tubes with micro pipette. We split enzyme solution $50{\mu}l$ into the tubes with melatonin direct kits and make them react at $37^{\circ}C$ for 2 hours. We split assay buffer $50{\mu}l$ into each tube and mixed melatonin tracer $50{\mu}l$ and melatonin antiserum $50{\mu}l$, respectively. After we made them react in room temperature, we decanted the superficial layer with a centrifuge and measured the activity for 1 minute by competitive method with ${\gamma}$-counter equipment. We draw a standard curve through logit-log graph with CPM(counts per minute) and counted the melatonin by B/B0. We conducted independent t-test to examine the homogeneous of melatonin value of before low-intensity and high-intensity exercise. We performed paired t-test to compare before and after low-intensity and high-intensity exercise, respectively. We carried out independent t-test to compare melatonin value after low-intensity and high-intensity exercise. Significance level was .05. RESULTS: The results were as follows; firstly melatonin was more increased in the group who was exposed to high intensity exercise when we compared before to after high and low intensity exercise, respectively. Secondly, high intensity exercise was more effective than low intensity exercise when we compared the two. CONCLUSION: In conclusion, secretion of melatonin which is the material of sleep improvement could be promoted by high intensity exercise. Low intensity exercise acted as a stress rather than improving sleep and had a negative effect on the secretion of melatonin because the melatonin was affected by stress.

Heating and Cooling Energy Demand Evaluating of Standard Houses According to Layer Component of Masonry, Concrete and Wood Frame Using PHPP (PHPP를 활용한 조적, 콘크리트, 목조 레이어 구성별 표준주택 냉·난방 에너지 요구량 평가)

  • Kang, Yujin;Lee, Junhee;Lee, Hwayoung;Kim, Sumin
    • Journal of the Korean Wood Science and Technology
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    • v.45 no.1
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    • pp.1-11
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    • 2017
  • A lot of the energy are consumed on heating and cooling in buildings. The buildings need to minimize the heating and cooling loads for $CO_2$ emissions and energy consumption reduction. In recently, also demand of detached houses were increase while the residential culture was changed. The structure of the domestic detached houses can be divided into masonry, concrete, wood frame houses. Therefore, in this study, the heating and cooling load and energy demand were analyzed on the equal area detached house consisting of three structural methods (Masonry, Concrete, Wood frame). Layer of wall, roof, and floor were composited by structure. Thermal transmittance (U-value) of each layer was using the PHPP calculation for considering stud, such as the wood frame wall. In addition, the case of without considering for studs in wood frame wall (Non-studs) was analyzed in order to compare the difference between studs or not. Analysis was performed using self-developed heating and cooling load calculation program (CHLC) based excel and ECO2. The results of cooling and heating load and energy demand showed the highest values in the wood frame structure, and the concrete structure were confirmed to maintain a high value secondly. Two structure were determined to be disadvantageous on the energy consumption. Consequently, the masonry structure have an advantage over the other structure under the identical conditions. It was determined that if the except for thermal bridges due to the studs in the wood frame structure, it can be reduced the energy consumption.

The Evolution of Makeup Methods of Korean Women in Response to Changing Standards of Beauty in the Early 20th Century (20세기 초 미의식의 변화에 따른 국내여성들의 화장법)

  • Lee, Soon-Jae
    • Journal of the Korean Society of Clothing and Textiles
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    • v.34 no.8
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    • pp.1364-1377
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    • 2010
  • Although the human body is a biological subject with definite and distinctive physical features, its actualization and perception differs among societies. The aesthetics of the human body are based on diverse cultural perceptions that must be considered prior to design development. This study establishes the foundations of newly adopted concepts of beauty that are presumed to have been established in the first half of the twentieth century that continue to affect our mindset even now. The research includes human figures in the articles of women's magazines and cosmetic advertisements in the early $20^{th}$ century. The results are as follows: First, the change of perception in the human body: Instead of being a subject of preservation, the body has become a subject of sculpture with emphasis on health in the 1920's and on beauty in the 1930's. The recognition of the importance of the body has created intensive attention on physical training and an increased sense of hygiene. The body exposed to the public perceives itself through the eyes of others that alter one's own perception of oneself as well as become a target of evaluation. There is an additional emphasis on the exotic eroticism of a passive subordinate. Western culture became the standard for modernization along with the dissociation of traditional standards and values. Through the effect of education and western thinking, the awareness of women's rights and self-appreciation was developed. Second, ideal beauty can be summarized as follows: Unprocessed natural beauty was extolled as ideal in the 1920's, but the 1930's, it highlighted big eyes and an aquiline nose that are the characteristics of western women. Taking care of one's appearance was recognized as an important value for every social class. Cosmetics and skin care treatments promised soft and white skin. In contrast to western cosmetics, dark and shiny hair was highly favored. Exercising and traveling, differing seasonal and regional skin treatments were also widely accepted. In its initial stages, the research had originally assumed that the beginning of the twentieth century would be a time in which traditional concepts of beauty and new, westernized aesthetics coexisted. However, as the research progressed, it was clear that the idea of beauty had already adopted occidental ideals by that time. Thus, it seems necessary to continue the study on the shifting paradigms of beauty that must have occurred in the nineteenth and late twentieth century.

Increased Vascular Endothelial Growth Factor in the Ventricular Cerebrospinal Fluid as a Predictive Marker for Subsequent Ventriculoperitoneal Shunt Infection : A Comparison Study among Hydrocephalic Patients

  • Lee, Jeong-Hyun;Back, Dong-Bin;Park, Dong-Hyuk;Cha, Yoo-Hyun;Kang, Shin-Hyuk;Suh, Jung-Keun
    • Journal of Korean Neurosurgical Society
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    • v.51 no.6
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    • pp.328-333
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    • 2012
  • Objective : The aim of this study is to determine the association between the cerebrospinal fluid (CSF) biomarkers and inflammation, and the predictive value of these CSF biomarkers for subsequent shunt associated infection. Methods : We obtained CSF samples from the patients with hydrocephalus during ventriculoperitoneal (VP) shunt operations. Twenty-two patients were enrolled for this study and divided into 3 groups: subarachnoid hemorrhage (SAH)-induced hydrocephalus, idiopathic normal pressure hydrocephalus (INPH) and hydrocephalus with a subsequent shunt infection. We analyzed the transforming growth factor-${\beta}1$, tumor necrosis factor-${\alpha}$, vascular endothelial growth factor (VEGF) and total tau in the CSF by performing enzyme-linked immunosorbent assay. The subsequent development of shunt infection was confirmed by the clinical presentations, the CSF parameters and CSF culture from the shunt devices. Results : The mean VEGF concentration (${\pm}$standard deviation) in the CSF of the SAH-induced hydrocephalus, INPH and shunt infection groups was $236{\pm}138$, $237{\pm}80$ and $627{\pm}391$ pg/mL, respectively. There was a significant difference among the three groups (p=0.01). Between the SAH-induced hydrocephalus and infection groups and between the INPH and infection groups, there was a significant difference of the VEGF levels (p<0.01). However, the other marker levels did not differ among them. Conclusion : The present study showed that only the CSF VEGF levels are associated with the subsequent development of shunt infection. Our results suggest that increased CSF VEGF could provide a good condition for bacteria that are introduced at the time of surgery to grow in the brain, rather than reflecting a sequel of bacterial infection before VP shunt.

Cytotoxicities of Tumor-specific T Lymphocytes Primed by Glioma Apoptotic Body - or Glioma Cell Lysate-pulsed Dendritic Cells

  • Kim, Jong-Tae;Chung, Dong-Sup;Kwak, Seung-Won;Han, Young-Min;Park, Young-Sup;Kim, Moon-Chan
    • Journal of Korean Neurosurgical Society
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    • v.38 no.2
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    • pp.126-131
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    • 2005
  • Objective : The choice of tumor antigen for dendritic cell[DC]-loading has still been an unresolved problem in the DC-based vaccine strategies against malignant gliomas that has not been found well-characterized tumor specific antigens. In this study, we compare tumor-specific T cell response induced by glioma apoptotic body[GAB]-pulsed DCs to response induced by glioma cell lysate-pulsed ones quantitatively. Methods : DCs generated in the presence of granulocyte macrophage-colony stimulating factor and interleukin[IL]-4 from peripheral blood mononuclear cells[PBMCs] of HLA-A2 positive healthy donors were cultured. Each GABs and glioma cell lysate generated from HLA-A2 positive T98G glioblastoma cells were co-incubated with DCs. $CD8^+$ T lymphocytes isolated from PBMCs of same donors were cultured in media containing IL-2 and either stimulated by GAB- or lysate-pulsed DCs three times at a weekly interval. The interferon[IFN]-${\gamma}$ concentrations of each cell culture supernate were measured by enzyme immunoassay technique. Cytolytic activity of the generated cytotoxic $CD8^+$ T cells either stimulated with GAB- or lysate-pulsed DCs was determined by a standard 4-h $^{51}Cr$-release assay. Results : IFN-${\gamma}$ production and cytolytic activity of effector T cells stimulated by GAB-pulsed DCs were significantly higher than those of T cells stimulated by lysate-pulsed ones. Conclusion : These results indicate the choice of antigen is a critical determinant in the induction of antitumor immunity against malignant glioma. Antigen preparations from GABs represent a promising alternative to glioma cell lysate in DC-based glioma vaccine strategies.