• Title/Summary/Keyword: standard cell

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Encapsulated Animal Cell Culture for the Production of Monoclonal Antibody(MAb)

  • Kim, Sung-Koo;Son, Jeong-Hwan;Yu, Sun-Hee
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.2 no.2
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    • pp.73-76
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    • 1997
  • Biopolymer membrane was prepared using two oppositely charged natural biopolymer. The biopolymer membrane was used for the encapsulation of two hybridoma cell lines(ATCC CRL-1606, ATCC BH-8852) to produce monoclonal antibodies. In order to reduce the down stream steps, the pore size of the membrane was controlled to retain the monoclonal antibodies in the capsules based on the diffusion experiments with standard proteins. T-flask culture showed cell densities of 8$\times$107cells/mL 3$\times$107cells/mL, and MAb concentrations of 506 $\mu\textrm{g}$/mL and 109$\mu\textrm{g}$/mL for encapsulated ATCC CRL-1606 and HB-8852, respectively. Two liter perfusion culture with encapsulated ATCC HB-8852 was performed to enhance the MAb production. The MAb production of the encapsulated hybridoma increased considerably comparing to the culture using silicone tubing for oxygen transfer.

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Development of Type3 Composite Cylinder for Fuel Cell Vehicle (연료전지 차량용 TYPE3 복합재 압력용기 개발)

  • Park, Ji-Sang;Cheung, Sang-Su;Chung, Jae-Han;Cho, Sung-Min;Kim, Tae-Wook
    • New & Renewable Energy
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    • v.4 no.3
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    • pp.51-57
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    • 2008
  • The objective of this study is to develop and validate a compressed hydrogen storage system for fuel cell vehicles. The type3 composite cylinder consists of full wrapped composites on a seamless aluminum liner. The key technologies, including design, analysis, and optimized fabrication process for 350bar composite cylinder, were established and verified, and the facilities for fabrication and validation testing have been constructed. Prototype cylinders were fabricated and validated through burst test and ambient cycling test in accordance with international standard.

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Estimation of Cell Concentration by Light Transmitter During the Culture of Methylotrophic Yeast Pichia pastoris

  • Choi, Du-Bok;Park, Enoch Y.S.;Lee, Yong-Bo;Na, Young-Hee;Lim, Chae-Kyu
    • Mycobiology
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    • v.31 no.4
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    • pp.226-228
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    • 2003
  • The multiple correlation coefficient between the values determined by dry weight and those determined by fluorometer was observed with r=0.96 and the standard error of calibration was 0.034. Using the best calibration data, in order to reconfirm the reliability of the fluorometer results in comparison with those obtained by dry weight on the cell concentration, fedbatch cultures were carried out. The results obtained by fluorometer measurements were in good agreement with those obtained by dry weight. The on-line monitoring of cell concentration by the fermentor system linked to a computer equipped with fluorometer was successfully carried out.

Fresnel lens optics simulation with middle sized linear concentration without secondary optics (2차 광학계가 필요없는 프레넬 렌즈를 이용한 중집광 광학계 시뮬레이션)

  • Kang, Sung-Won;Kim, Yong-Sik;Sim, Chang-Ho
    • 한국태양에너지학회:학술대회논문집
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    • 2011.11a
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    • pp.27-33
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    • 2011
  • HCPV(High Concentrated PV) systems have well known for CPV market all over the world. Low concentration type silicon based modules have been introduced in the market. But low cost of standard flat silicon modules made them useless nowadays. High cost of compound semiconductor solar cell reduced cost effective cpv module production than that of recently silicon solar cell. In order to overcome increasing cost of CPV module, we study middle concentration type fresnel lens simulation using concentrated type silicon based solar cell. Linear type fresnel lens made production of CPV module without secondary optics such as light pipe or light tunnel. This type of fresnel lens design makes more cost effective solution for cpv niche market.

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Synthesis of Novel Organic Dyes Containing Coumarin Moiety for Solar Cell

  • Choi, Hyun-Bong;Baik, Chul;Kim, Hyun-Jun;Kim, Jeum-Jong;Song, Ki-Hyung;Kang, Sang-Ook;Ko, Jae-Jung
    • Bulletin of the Korean Chemical Society
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    • v.28 no.11
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    • pp.1973-1979
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    • 2007
  • Novel organic dyes, JK-34, JK-35 and JK-36 containing bis-dimethylfluorenyl amino coumarin unit are designed and synthesized. Nanocrystalline TiO2 dye-sensitized solar cells were fabricated using these dyes. Under standard global AM 1.5 solar condition, the JK-34 sensitized cell gave a short circuit photocurrent density of 10.05 mA cm?2, open circuit voltage of 0.65 V, and a fill factor of 0.68, corresponding to an overall conversion efficiency η of 4.54%. We found that the power conversion efficiency was shown to be quite sensitive to the structural modifications of bridging thiophene moiety.

Photovoltaic Characteristics of Low-density Concentration GaAs Solar Cells with/without Anti-reflective Coating

  • Noh, Sam Kyu;Kim, Jong Soo;Kim, Jin Soo;Yu, Jae Su
    • Applied Science and Convergence Technology
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    • v.23 no.1
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    • pp.27-33
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    • 2014
  • We have studied photovoltaic characteristics of single-junction GaAs solar cells with/without an $MgF_2/ZnS$ anti-reflective coating (ARC) illuminated by low-density concentration (<10 suns). By the ARC deposition, the short-circuit current density ($J_{SC}$) and the fill factor (FF) are increased by $5mA/cm^2$ and 5% at a standard illumination (1 sun), respectively, and the resulted conversion efficiency is enhanced by 45%. In contrast with the cell with no ARC showing a rapid degradation with increasing concentration power, the efficiency of ARC-deposited cell remains almost constant as ($17.7{\pm}0.3$)% regardless of the concentration. It informs that ARC treatment is very effective in GaAs concentrator solar cells.

Virus-cell Fusion Inhibitory Activity for the Polysaccharides from Various Korean Edible Clams

  • Woo, Eun-Rhan;Kim, Wan-Seok;Kim, Yeong-Shik
    • Archives of Pharmacal Research
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    • v.24 no.6
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    • pp.514-517
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    • 2001
  • In order to find potent virus-cell fusion inhibitory components from Korean edible clams, thirteen prepared polysaccharides were introduced to syncytia formation inhibition assay, which is based on the interaction between the HIV-1 envelope protein gp 120/41 and the cellular membutane protein CD4 of T lymphocytes. Among them, Meretrix petechialis showed a potent viruscell fusion inhibitory activity. Fusion index (F1) and percent (%) fusion inhibition of the polysaccharide of this clam were $0.21{\pm}0.02$, and $67.52{\pm}4.09$ at 100781m1, respectively. It exhibited almost equivalent virus-cell fusion inhibitory activity to that of dextran sulfate which was used as a standard control.

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A Meta-Analysis Comparing Lobectomy versus Segmentectomy in Stage I Non-Small Cell Lung Cancer

  • Lim, Tae Yoon;Park, Samina;Kang, Chang Hyun
    • Journal of Chest Surgery
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    • v.52 no.4
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    • pp.195-204
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    • 2019
  • Lobectomy is considered the standard strategy for early-stage non-small cell lung cancer (NSCLC). However, sublobar resection for NSCLC has recently received increased attention. The objective of this study was to compare 5-year survival, recurrence-free survival, postoperative mortality, and postoperative morbidities in patients who received segmentectomy versus those who received lobectomy through a meta-analysis. Sixteen studies were included and the combined hazard ratios or odds ratios were calculated. The results revealed that the 5-year survival rate after segmentectomy was comparable to that of lobectomy for stage IA NSCLC. However, segmentectomy for stage I NSCLC had lower rates of postoperative mortality and morbidities than lobectomy.

Small Cell Communication Analysis based on Machine Learning in 5G Mobile Communication

  • Kim, Yoon-Hwan
    • Journal of Integrative Natural Science
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    • v.14 no.2
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    • pp.50-56
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    • 2021
  • Due to the recent increase in the mobile streaming market, mobile traffic is increasing exponentially. IMT-2020, named as the next generation mobile communication standard by ITU, is called the 5th generation mobile communication (5G), and is a technology that satisfies the data traffic capacity, low latency, high energy efficiency, and economic efficiency compared to the existing LTE (Long Term Evolution) system. 5G implements this technology by utilizing a high frequency band, but there is a problem of path loss due to the use of a high frequency band, which is greatly affected by system performance. In this paper, small cell technology was presented as a solution to the high frequency utilization of 5G mobile communication system, and furthermore, the system performance was improved by applying machine learning technology to macro communication and small cell communication method decision. It was found that the system performance was improved due to the technical application and the application of machine learning techniques.

Aggressive cutaneous squamous cell carcinoma of the scalp

  • Suk, Sangwoo;Shin, Hyun Woo;Yoon, Kun Chul;Kim, Junekyu
    • Archives of Craniofacial Surgery
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    • v.21 no.6
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    • pp.363-367
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    • 2020
  • Cutaneous squamous cell carcinoma (cSCC) is the second most common nonmelanoma skin cancer, and its incidence is increasing globally. In Korea, there were 12,516 diagnosed cases of cSCC between 1999 and 2014. Surgical treatment, for which several options are available, is the standard of care for cSCC and securing a sufficient surgical resection margin is always important. cSCC of the scalp sometimes exhibits unusually aggressive behavior. In this article, we report a case of cSCC of the scalp with invasion into the skull and dura mater.