• Journal of Korea Port Economic Association
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• v.26 no.3
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• pp.114-129
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• 2010

Busan port's main function is handling container cargo compared to world major ports and the percentage of handling general cargo such as dirty bulk cargo is very low. In other words, although total cargo weight of Busan port that recorded the handling result of 13.29million TEU in 2008 reached 113.05million ton, total cargo weight of general cargo was 15.31million ton, so container cargo accounted for 88.1% of whole cargo weight. However, it is the time to create high added value by the increase of handling and marketing dirty bulk cargo. Originally, the dirty bulk cargo was not the avoided object from the first. Somehow, it is a very high added value cargo, and is surely essential strategic material to basic industries of nation. However, it becomes dirty bulk cargo as the companies are reluctant to handle it because of environmental problem, distinct characteristic in handling, uncertain break even point due to imbalance between supply and demand compared to container cargo. However, items that are classified as dirty bulk cargo now are certainly necessary strategic materials to national basic industries or national life. Besides it seems to be a high added value cargo here and now. Therefore, it is time that increasing of handling dirty bulk cargo by marketing it and the system for efficient handling such as constructing the exclusive use wharf in Busan port, modernizing of facilities and equipments, stable secure of place for holding and handling through development of distribution complex by item, efficient data processing and closer cooperation by setting up a SCM of related authorities are needful.

• Composites Research
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• v.31 no.5
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• pp.209-214
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• 2018

This paper explain about the development of environmental friendly, low cost and stable supply material i.e., rice husk and shell were used as micro incorporating bio waste filler. Those were processed by ball mill and analyzed through micro observation by FE-SEM, EDS and particle size distribution. The obtained filler was mixed with epoxy resin for the manufacturing of CFRP composite and study tensile properties. In EDS analysis main contents of rice husk and rice husk ash are C, O and Si. When rice husk was burned C and Si ration were increased. Shell powder has C, O and Ca. It caused $CaCO_3$ from shell. Surface weighted mean of rice husk powder is $6.19{\mu}m$ and volume weighted mean is $14.77{\mu}m$. And it has rod type particles which caused hair and husk structure parts. Surface weighted mean of rice husk ash powder is $1.55{\mu}m$ and volume weighted means is $8.20{\mu}m$. Surface weighted mean of shell powder is $2.53{\mu}m$ and volume weighted mean is $5.79{\mu}m$. The tensile decreased with increasing the content of micro filler in CFRP composites. In case of rice husk, the significant decrement of tensile strength was observed. and in case of shell powder, there is no effect of changes take place in tensile strength.

• Journal of Korean Society of Forest Science
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• v.96 no.1
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• pp.107-114
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• 2007

Establishments of the seedlings inside the natural forest from adjacent artificial forests would be an important factor in forest stand dynamics. This study was conducted to see the invasion of Korean pine (Pinus koraiensis) seedlings which is not native in this region, into the natural deciduous broad-leaved forest in Gwangneung, Korea. There is no mother tree at the I ha study site while the number of naturally regenerated P. koraiensis seedlings was 345 trees and 56% of them were clumped with more than two seedlings at each point. Applying the image segmentation method to IKONOS satellite image of January, 2003, the distance from the center of 1 ha study site to the nearest mother tree and plantation of Korean pine were 200 m and 270 m, respectively. The average height and root-collar diameter of the seedlings were 34 em and 7 mm, respectively and the age of 207 seedlings (60%) were below 5 years old. Most abundant range of soil moisture gradient and LAl (leaf area index) were from 16 to 20% and those of LAI were from 3.1 to 3.5. To understand the dynamics and seed dispersal pattern of Korean pine in the Gwangneung natural deciduous broad-leaved forests, additional studies not only long-term monitoring of growth and mortality of naturally regenerated Korean pine seedlings but also application of stable isotope analysis and molecular genetic techniques was recommended.

• Proceedings of the Microbiological Society of Korea Conference
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• 1991.04a
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• pp.225-236
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• 1991

The genes encoding the thermostable $\alpha$-amylase and maltogenic amylase from Bacillus lichenciformis were cloned and expressed in E. coli. The recombinant plasmid pTA322 was found to contain a 3.1kb EcoRI genomic DNA fragment of the thermostable $\alpha$-amylase. The cloned $\alpha$-amylase was compared with the B. licheniformis native $\alpha$-amylase. Both $\alpha$-amylase have the same optimal temperature of $70^{\circ}C$ and are stable in the pH range of 6 and 9. The complete nucleotide sequences of the thermostable $\alpha$-amylase gene were determined. It was composed of one open reading rame of 1,536 bp. Start and stop codons are ATG and TAG. From the amino acid sequence deduced from the nucleotide sequence, the cloned thermostable $\alpha$-amylase is composed of 483 amino acid residues and its molecular weight is 55,200 daltons. The content of guanine and cytosine is $47.46mol\%$ and that of third base codon was $53_41mol\%$. The recombinant plasmid, pIJ322 encoding the maltogenic amylase contains a 3.5kb EcoRI-BamHI genomic DNA fragment. The optimal reaction temperature and pH of the maltogenci amylase were $50^{\circ}C$ and 7, respectively. The maltogenic amylase was capable of hydrolysing pullulan, starch and cyclodextrin to produce maltose from starch and panose from pullulan. The maltogenic amylase also showed the transferring activity. The maltogenic amylase gene is composed of one open reading frame of 1,734bp. Start and stop codons are ATG and ATG. At 2bp upstream from start codon, the nucleotide sequence AAAGGGGGAA seems to be the ribosome-binding site(RBS, Shine-Dalgarno sequence). A putative promoter(-35 and-10 regions) was found to be GTTAACA and TGATAAT. From deduced amino acid sequence from the nucleotide srquence, this enzyme was comosed of 578 amino acid residues and its molecular weight was 77,233 daltons. The content of guanine and cytosine was $48.1mol\%$. The new recombinant plasmid, pTMA322 constructed by inserting the thermostable $\alpha$-amylase gene in the EcoRI site of pIJ322 to produce both the thermostable $\alpha$-amylase and the maltogenic amylase were expressed in the E. coli. The two enzymes expressed from E. coli containing pTMA322 was reacted with the $15\%$ starch slurry at $40^{\circ}C$ for 24hours. The distribution of the branched oligosaccharides produced by the single-step process was of the ratio 50 : 50 between small oligosaccharide up DP3 and large oligosaccharide above DP3.

• Microbiology and Biotechnology Letters
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• v.41 no.3
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• pp.320-326
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• 2013

In a previous study, we investigated the antioxidative and cellular protective effects of Parthenocissus tricuspidata stem extracts. In this study, we prepared nano-emulsion containing P. tricuspidata stem extract to improve skin permeation. The particle size of the nano-emulsion using the microfluidizer was 302 nm. Its loading efficiency was over 86%. The size distribution of the nano-emulsion took a monodispersed form and the nano-emulsion was more stable than typical emulsion without using microfluidizer during a 2 week period. In vitro skin permeation study of nano-emulsion containing P. tricuspidata stem extracts was carried out using Franz diffusion cell. The 1,3-butylene glycol used as a control group had 32.59% skin permeation efficiency. The skin permeation efficiency of the nano-emulsion was 42.47%. Also, we observed the antibacterial activity of the ethyl acetate fraction on skin flora for prospective applications as a natural antimicrobial. The ethyl acetate fraction had antibacterial activities higher than methyl paraben on Staphylococcus aureus, and Bacillus subtilis. These results indicate that nano-emulsion containing P. tricuspidata stem extracts could possess valued applications in cosmetic formulations for improving skin permeation. Also, based on the antibacterial activities on skin flora, antioxidative and cellular protective effects shown in our previous study, we suggest that P. tricuspidata stem extracts could be used as functional cosmetic materials.

• Journal of Energy Engineering
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• v.10 no.3
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• pp.223-232
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• 2001

To study the stabilization characteristics of diffusion flame formed in the wake of a cylindrical bluff body with fuel injection, the flame stability limits, length and temperature of recirculation zone of flame, turbulence intensity distribution near the recirculation zone of flame were measured and analyzed. The length of recirculation zone is independent on main fuel injection quantity, but it is dependent on fuel injection angles, air stream velocity, and auxiliary fuel injection into recirculation zone. For diffusion flame, in general, the flame stabilization is deteriorated with increase of he length of recirculation zone, but if the turbulence generator is installed, the flame stabilization is improved with increase of the length of recirculation zone. The temperature of recirculation zone is dependent on fuel injection angles, auxiliary fuel injection into recirculation zone, turbulence generators, and it dependent on fuel injection angles, auxiliary fuel injection into recirculation zone, turbulence generators, and it has a maximum value at the condition of each theoretical mixture. In general, the more temperature of recirculation zone is low, the more flame is stable. But when the turbulence generator is installed, the more temperature of recirculation zone is low, the more flame is unstable. The turbulence intensity in the wake of bluff body is increased with increase of diameter or blockage ratio of grid. The more turbulence intensity is increased by installation of turbulence generator, the more flame is unstable. Finally, It is clear that the stabilization characteristics of diffuser flame can be controlled by some parameters such as fuel injection angles, auxiliary fuel injection into recirculation zone, turbulence generators.

• Journal of Bio-Environment Control
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• v.15 no.2
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• pp.138-148
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• 2006

This study was carried out to evaluate the structural safety fur both the attached wind-protecting wall in greenhouse and the detached one installed outside. Regarding the attached wind-protecting wall in greenhouse, the analysis was conducted by doing a fluid-structure coupled field analysis using both CFX-5.7 and ANSYS 8.1 and also under the design condition of an instantaneous maximum wind velocity of $30.9m{\cdot}s^{-1}$. Three kinds of the width ranged from 30 to 90cm were considered in this study. With regard to the detached wind-protecting wall, the structural saffty was analyzed under the pressure difference of 1,117 Pa which corresponded to a wind velocity of $50m{\cdot}s^{-1}$ and the analytical results were also compared with theoretical ones. The result showed that there was little difference in the distribution of velocity overall and total pressure on the lateral side according to the width of the attached wind-protecting wall, but greenhouse with wind-protecting widths of 30 to 60cm has been reinforced to the extent of about 11% when compared with the case of being without the wall. The result also showed that the detached wind-protecting wall with a main-column interval of 3m was not stable so that it was necessary for the detached wind-protecting wall to be adequately reinforced to secure structural stability. Finally, there was great difference between analytical results and theoretical studies. The difference meant that there was some possibility of including errors when a theoretical study was done in three dimensional structure.

• KOMUNHWA
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• no.70
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• pp.35-62
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• 2007

Songguk-ri site gained academic recognition by the excavation of stone coffin tomb with a bronze dagger in 1974. And it is confirmed that this site is epoch-making in Korean bronze age through the following excavation started in 1975. But the excavation reports published until now do not have even overall view of this site, thus it is difficult to get the whole picture of this site. Thus, in this paper the author reexamined all reports on this site and by making the map of overall view and distribution of archaeological features as a basic research. Moreover, I analyzed also artifacts from this site, and compared with the recent papers written by other researchers about the chronology and character. Songguk-ri site has livelihood domain which consists of dwelling pits, attached features, storage pits, pot-firing features, wood fence, abatises, buildings above ground, as well as cemetery which consists of stone-coffins, jar-coffins, pit tombs. Trace of making large terrace was also excavated. These features seem to belong to the same archaeological stage, dated about B.C.850-550, according to C14 dating. On the other hand, the intensification of wet-rice cultivation made this group more productive. Based on this financial strength, some influential group emerged they constructed defensive settlement to protect their products safely. Besides it seems that there were frequent occurrence of conflicts. However, we can know that they kept their stable life, through the expansion of living space. Consequently, Songguk-ri site played a role of the summit among some settlement in this area.

• Journal of Korea Entertainment Industry Association
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• v.14 no.8
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• pp.337-344
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• 2020

The purpose of this study was to investigate the effect of theratainment low extremity complex exercise using unstable surface on knee and static plantar pressure in patient with genu varum. 25 adult subjects were recruited and randomized into stable surface exercise group(SEG) and unstable surface exercise group(UEG). Subjects carried out complex exercise(elastic band exercise & squat exercise) for 40 minutes, 4 times per week for 5 weeks. The knee joint distance and Q-angle of all subjects measured, the foot pressure measured distribution on the front and rear of both foots during pre and post. The most outcome at post was significantly improved than the pre outcome in the both group(p<.05). The result of this study show that the low extremity complex exercise using unstable surface may be appropriate for improving structure of knee joint and static plantar pressure in patient with genu varum. This will enable the application of low extremity complex exercise using unstable surface in rehabilitation therapy of genu varum and it will help guide the selection of the therapist as one therapeutic basis.

• Journal of Radiopharmaceuticals and Molecular Probes
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• v.7 no.2
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• pp.105-111
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• 2021

⁸⁹Zr is a positron-emitting radioisotope, which has known as well-suited radioisotope for use in a monoclonal antibody-based imaging agent for immuno-PET. The purpose of this study was to quantitatively evaluate the diagnostic ability of general trastuzumab and thio-trastuzumab as HER2 positive receptors based on Df hexadentate iron chelator. Desferrioxamine-p-SCN (Df-Bz-NCS) and desferroixamine-maleimide (Df-Mal) were purchased from Macrocyclics (Dallas, TX, USA). The trastuzumab was purchased from Roche (Schweiz), and thio-trastuzumab was obtained from professor Hyo-Jeong Hong group (Kangwon National University). The radioisotope ⁸⁹Zr was produced by domestic purification system and KIRAMS using medical cyclotron (50 MeV, Scantronix). The conjugates of Df-trastuzumab and Df-thio-trastuzumab were prepared with Df-Bz-NCS and Df-Mal under basic aqueous solution (pH 8-9) at room temperature, respectively. The conjugates purified by PD-10 column were mixed with dried ⁸⁹Zr chloride. ⁸⁹Zr-labeled conjugates were purified and concentrated by Amicon ultra centrifugal filter. The preparation step and time of ⁸⁹Zr-labeled conjugates was shorted as 4 steps within 2 hours. ⁸⁹Zr-labeled conjugates showed the highly radiochemical purity of over 98%, and were very stable until 7 days by the analysis of radio-ITLC method. Each radio-labeled conjugates were also exhibited the highly stability in both PBS buffer and mouse serum. Immuno-PET imaging of ⁸⁹Zr-labeled conjugates in mice bearing gastric cancer xenograft tumors with HER2 expression showed high tumor uptake in the NCI-N87 HER2-expressing. However, ⁸⁹Zr-Df-Mal-thio-trastuzumab showed a relatively lower tumor-to-background ratio than ⁸⁹Zr-Df-Bz-trastuzumab, as well as whole-body distribution. In the results, ⁸⁹Zr-Df-Bz-trastuzumab was evaluated to have a relatively higher HER2 diagnostic ability than ⁸⁹Zr-Df-Mal-thio-trastuzumab.