• Title/Summary/Keyword: stable degree

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Enzymatic Properties of Protease from the Hepatopancreas of Shrimp, Penaeus japonicus

  • Kim Hyeung-Rak
    • Fisheries and Aquatic Sciences
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    • v.3 no.3_4
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    • pp.188-194
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    • 2000
  • A protease purified from hepatopancreas of shrimp, Penaeus japonicus, had maximum activity at $70^{\circ}C$ and in neutral and alkaline pH ranges. Specific activity at optimum reaction condition of the protease was estimated to be approximately 12 U/mg/min. The protease was stable in neutral and alkaline pH ranges and activity was retained after heat treatment at $50^{\circ}C$ for 30 min. Apparent $K_m$ and $V_{max}$ value against casein substrate were estimated to be $0.29\%$ and $7.8see^{-1}$, respectively, and those against N-CBZ-L-tyrosine p-nitropheny1 ester (CBZ­Tyr-NE) were 0.38 mM and $2,400 see^{-1}$, respectively. The N-termina1 sequence of the protease showed high homology to the trypsin from same species and the proteases from shrimp. Myosin heavy chain (MHC) from shrimp tail meat was the most susceptible to the protease and actin/tropomyosin were degraded progressively during 4 hr incubation, but to a lesser degree than MHC.

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Dehydropolycondensation of Aminopenols under the Catalytic Action of Metallic Chelate Compounds (I) Effects of the Solvents and Characteristics of the Oligomers Obtained (金屬킬레이트化合物의 觸媒作用에 依한 Aminophenol 類의 酸化的 重縮合反應 (Ⅰ) 溶媒의 效果와 生成重合體의 特性)

  • Choi, Kyu-Suck
    • Journal of the Korean Chemical Society
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    • v.12 no.3
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    • pp.114-120
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    • 1968
  • Fe-EDTA complex, which is easily formed when Fe salt and EDTA.2Na are mixed in the aqueous medium, is found to be a very effective catalyst in the dehydropolycondensation of aminophenols. In the dehydropolycondensation of aminophenols, the catalyst, Fe(Ⅲ)-EDTA complex (higher oxidation state) is reduced to less stable Fe(Ⅱ)-EDTA complex (lower oxidation state), and the latter is easily oxidized by air to the original higher oxidation state complex, therefore the catalytic action of Fe-EDTA complex is found to be recycled effectively. Under the catalytic action of the above mentioned complex, p-aminophenol is polymerized in the aqueous medium to form the oligomers of p-aminophenol, which the degree of polymerization to be 5 or more. The oligomers formed contain partly quinone nucleus as well as amino and hydroxyl groups. In this study, the effects of the solvents and characteristics of the oligomers are discussed. These types of polymerizations catalyzed by the metallic chelate compounds are considered to be very closely related to the reactions in the living matters.

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A New Penalty Parameter Update Rule in the Augmented Lagrange Multiplier Method for Dynamic Response Optimization

  • Kim, Min-Soo;Choi, Dong-Hoon
    • Journal of Mechanical Science and Technology
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    • v.14 no.10
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    • pp.1122-1130
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    • 2000
  • Based on the value of the Lagrange multiplier and the degree of constraint activeness, a new update rule is proposed for penalty parameters of the ALM method. The theoretical exposition of this suggested update rule is presented by using the algorithmic interpretation and the geometric interpretation of the augmented Lagrangian. This interpretation shows that the penalty parameters can effect the performance of the ALM method. Also, it offers a lower limit on the penalty parameters that makes the augmented Lagrangian to be bounded. This lower limit forms the backbone of the proposed update rule. To investigate the numerical performance of the update rule, it is embedded in our ALM based dynamic response optimizer, and the optimizer is applied to solve six typical dynamic response optimization problems. Our optimization results are compared with those obtained by employing three conventional update rules used in the literature, which shows that the suggested update rule is more efficient and more stable than the conventional ones.

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Characterization of Photoresist Processing by Statistical Design of Experiment (DOE)

  • Kim, Gwang-Beom;Park, Jae-Hyun;Soh, Dae-Wha;Hong, Sang-Jeen
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2005.11a
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    • pp.43-44
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    • 2005
  • SU-8 is a epoxy based photoresist designed for MEMS applications, where a thick, chemically and thermally stable image is desired. But SU-8 has proven to be very sensitive to variation in processing variables and hence difficult to use in the fabrication of useful structures. In this paper, negative SU-8 photoresist processed has been characterized in terms of delamination. Based on a full factorial designed experiment. Employing the design of experiment (DOE), a process parameter is established, and analyzing of full factional design is generated to investigate degree of delamination associated with three process parameters: post exposure bake (PEB) temperature, PEB time, and exposure energy. These results identify acceptable ranges of the three process variables to avoid delamination of SU-8 film, which in turn might lead to potential defects in MEMS device fabrication.

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해양에서 분리한 Bacillus subtilis SH-1이 분비하는 용균효소의 정제 및 특성

  • 진성현;정영기;류병호
    • Microbiology and Biotechnology Letters
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    • v.24 no.2
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    • pp.191-196
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    • 1996
  • The bacteriolytic enzyme produced from Bacillus subtilis SH-1 was purified and characterized, and its molecular weight was determined. The bacteriolytic enzyme activity was increased about 66.5 times via purification with recovery yield of 18.5%. The optimum pH and temperature of this enzyme were 9.0 and 50$\circ$C. The enzyme was stable within a pH range of 6.0-10.0 and unstable above 60 . The molecular weight of the enzyme was estimated to be 23,000 dalton in a form of monomer with no other subunits. Effect of the enzyme on the lysis of bacteria engaged in food posion was tested. The lysis degree was below 31% against Gram negative bacteria and above 48% in Gram positive bacteria. The values higher than 73% were obtained against Vibrio sp. and Listeria sp. As the turbidity of dissolved peptidoglycan clecreases, the free amino group levels were increased. And, based on hydrolysis of casein, this enzyme was thought to be an endopeptidase.

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Development of Biped Walking Robot Capable of Supporting Heavy Weight (고중량 지지 가능한 이족보행로봇의 개발)

  • Choi H.S.;Lee S.J.;Oh J.H.;Kang Y.H.
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2006.05a
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    • pp.63-64
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    • 2006
  • In this paper, design modification was performed to improve the structure of ex-developed 12 D.O.F Biped walking robot, KUBIR-1 similar with human beings. The motion of KUBIR-1 was slow and had a limited walking space. Hence I designed an improved BWR named KUBIR-2 with 12 degree of freedom. KUBIR-2 was designed to solve the following problems of KUBIR-1. First, KUBIR-2 was more simply designed in the four-bar-link mechanism, and its weight was reduced. Second, it had the built-in controller and motor driver. Third, walking velocity of KUBIR-2 was increased by improvement of speed and motion joint angle range. In addition to these, we modified the structure of the foot for more stable walking.

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Effect of S on Arc Stability of MAG Welding (MAG용접의 Arc안정성에 미치는 S의 영향)

  • 안영호;이종봉;엄동석
    • Journal of Welding and Joining
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    • v.17 no.1
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    • pp.55-61
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    • 1999
  • The effect of S content in welding wires on the arc stability was investigated, in the region of short circuit transfer and spray transfer. In the region of short circuit transfer, with increasing S content, average arcing time and average short circuit time were decreased. Therefore, droplet transfer frequency was increased, due to the reduction of arcing time and peak current at the moment of arc-reiginition was decreased, due to the decrease of short circuit time. In the region of spray transfer, the fluctuation degree of arc current and arc voltage became more stable, with increasing S content.

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Development & Verification of Frequency-Strain Dependence Curve (주파수-변형률 곡선의 개발 및 검증)

  • Jeong, Chang-Gyun;Kwak, Dong-Yeop;Park, Du-Hee
    • Proceedings of the Korean Geotechical Society Conference
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    • 2009.03a
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    • pp.146-153
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    • 2009
  • One dimensional site response analysis is widely used in prediction of the ground motion that is induced by earthquake. Equivalent linear analysis is the most widely used method due to its simplicity and ease of use. However, the equivalent linear method has been known to be unreliable since it approximates the nonlinear soil behavior within the linear framework. To consider the nonlinearity of the ground at frequency domain, frequency dependent algorithms that can simulate shear strain - frequency dependency have been proposed. In this study, the results of the modified equivalent linear analysis are compared to evaluate the degree of improvement and the applicability of the modified algorithms. Results show the novel smoothed curve that is proposed by this study indicates the most stable prediction and can enhance the accuracy of the prediction.

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Anesthetic considerations for a pediatric patient with Wolf-Hirschhorn syndrome: a case report

  • Tsukamoto, Masanori;Yamanaka, Hitoshi;Yokoyama, Takeshi
    • Journal of Dental Anesthesia and Pain Medicine
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    • v.17 no.3
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    • pp.231-233
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    • 2017
  • Wolf-Hirschhorn syndrome is a rare hereditary disease that results from a 4p chromosome deletion. Patients with this syndrome are characterized by craniofacial dysgenesis, seizures, growth delay, intellectual disability, and congenital heart disease. Although several cases have been reported, very little information is available on anesthetic management for patients with Wolf-Hirschhorn syndrome. We encountered a case requiring anesthetic management for a 2-year-old girl with Wolf-Hirschhorn syndrome. The selection of an appropriately sized tracheal tube and maintaining intraoperatively stable hemodynamics might be critical problems for anesthetic management. In patients with short stature, the tracheal tube size may differ from what may be predicted based on age. The appropriate size ( internal diameter ) of tracheal tubes for children has been investigated. Congenital heart disease is frequently associated with Wolf-Hirschhorn syndrome. Depending on the degree and type of heart disease, careful monitoring of hemodynamics is important.

A Diagnostic Method of Control-in/out in the Glass Furnace

  • Cho, Jin-Hyung;Lee, Sae-Jae;Jang, Do-Soo
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.29 no.1
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    • pp.151-154
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    • 2006
  • The high degree of viscosity and the non-Newtonian fluid dynamics characterizes the process inside a glass furnace. Because the temperature is fluctuating in very short time-intervals, it is hard to determine that the status of its fluctuation is stable or unstable. Usually Shewhart-chart is used to determine the control status. However because of the characteristics of the temperature fluctuations in the glass furnace it does not directly serve the purpose here. Therefore we suggest using ARIMA to diagnose control status and confirm that the method using ARIMA can be a better tool than Shewhart-chart.