• Plant Biotechnology Reports
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• 제2권1호
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• pp.75-85
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• 2008

Prune dwarf virus (PDV) is an Ilarvirus systemically infecting almond trees and other Prunus species and spreading through pollen, among other means. We have studied strategies based on coat protein (cp) gene to block PDV replication in host plant cells. A Portuguese isolate of PDV was obtained from infected almond leaves and used to produce the cDNA of the cp gene. Various constructs were prepared based on this sequence, aiming for the transgenic expression of the original or modified PDV coat protein (cpPDVSense and cpPDVMutated) or for the expression of cpPDV RNA (cpPDVAntisense and cpPDVwithout start codon). All constructs were tested in a PDV host model, Nicotiana benthamiana, and extensive molecular characterization and controlled infections were performed on transformants and their progenies. Transgenic plants expressing the coat protein RNA were able to block the proliferation of a PDV isolate sharing only 91% homology with the isolate used for cpPDV cloning, as evaluated by DAS-ELISA on newly developed leaves. With cp expression, the blockage of PDV proliferation in newly developed leaves was only achieved with the construct cpPDV Mutated, where the coat protein has a substitution in the 14th aa residue, with arginine replaced by alanine. This result points to a possible role of the mutated amino acid in the virus ability to replicate and proliferate. This work reveals the possibility of achieving protection against PDV through either coat protein RNA or mutated cp sequence.

• 방송공학회논문지
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• 제19권4호
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• pp.491-501
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• 2014

최근 전 세계적으로 지진, 쓰나미와 같이 막대한 인명과 재산 피해를 야기시키는 자연재난이 증가함에 따라 재난에 효과적으로 대응하기 위한 방송 시스템에 대한 요구가 증가하고 있다. 재난 방송시스템에서는 재난 발생 시 재난정보를 보다 빠르고 효과적으로 전달하기 위하여, 단말을 깨워 재난 방송 채널로 자동 변환 시켜주는 대기모드해제(wake-up) 신호가 사용된다. 본 논문에서는, 재난 방송시스템을 위한 대기모드해제 검출 방법을 제안한다. 재난방송용 대기모드 해제신호는 기존 디지털TV 시스템과의 간섭을 줄이기 위하여 저전력/협대역에서 고려되어야 하며, 좋은 자기 상관 특성을 가져야한다. 우선, 본 논문에서의 자기상관특성이 우수한 단일 m-시퀀스와 상보부호(complementary code)를 이용한 대기모드해제의 적합성을 분석하고, 신호 대 잡음비에 따른 성능을 검증한다. 다음으로 대기모드 해제신호의 검출 성능을 향상시키기 위하여, 의사잡음 계열의 Barker 부호 및 Walsh-Hadamard 부호를 이용한 직접확산 방식의 신호 전송 포맷을 제안한다. 제안된 직접확산방식에 의한 대기모드 해제신호 검출 방법은 확산 이득으로 인하여 단일 m-시퀀스와 Golay 부호(이진 상보부호)를 이용한 방법보다 뛰어난 검출 성능을 나타낸다.

• 대한전자공학회논문지TC
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• 제46권8호
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• pp.1-9
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• 2009

본 논문은 무인기용 대전자전 송수신 장치 개발에 대한 연구로 재밍(jamming) 환경에 강건한 대역확산(DSSS) 송수신기 개발에 대해 기술한다. 대역확산 방식은 데이터의 전송률 보다는 재밍마진이 중요하기 때문에 송수신 장치의 개발 목표로 재밍마진이 15dB 이상이고, 확산코드를 변경 가능한 송수신기를 제작하는 것이다. 멀티패스 성분을 이용하기 위한 레이크 수신기와, FEC로 부호이득이 7.2dB인 터보 코드를 적용하였다. 본 논문에서는 대역확산 송수신 장치의 전체적인 구조와 설계방법, 기능시험 결과를 기술하고 결론을 맺는다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.114.2-115
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• 2003

Rice stripe virus causes severe damage to rice in Korea, Japan and China. RSV is a type member of the tenuivirus group and transmitted by the small brown planthopper, Laodeiphax striatellus, in a persistant manner. Until now, occurrence of RSV is limited in of southern part of Korea. But recently occurrence of RSV is increasing and spreading in central part of Korea including Chungcheong and Kyonggi province. So we analyzed recent occurrence trend of RSV which is increased and cloned and sequenced coat protein gene for isolating of RSV strain. Infected rice of each species(Ilpumbyeo, Sindongjinbyeo, Keumobyeo-2, Dongjinbyeo, Jongnambyeo, Samcheonbyeo, etc.)is collected, we extracted total RNA from infected leaves and detected RSV viral RNA by reverse transcription(RT)-PCR using specific primer of coat protein gene. The result of RT-PCR, we observed specific band. We already cloned cDNA from the band, is analyzing sequence variety and homology of each species.

• 한국환경농학회지
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• 제28권4호
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• pp.435-441
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• 2009

In the present study, we analyzed the defensin protein deduced from Korean radish (Raphanus sativus L.) seeds.To express the genes in E. coli, we constructed a recombinant expression vector with a defensin gene, named rKRs-AFP gene isolated from Korean radish seeds. Over expressed rKRs-AFP proteins was separated by SDS-PAGE to determine the purity, and protein concentration was determined by the Bradford method. Antifungal activity was assessed by disk assay method against the tested fungi. As a result, when 500 mL of cell culture were disrupted by sonicator, 32.5 mg total proteins were obtained. The purified protein showed a single band on SDS-PAGE with estimated molecular weight about 6 KDa, consistent with the molecular mass calculated from the deduced amino acid sequence. The purified rKRs-AFP protein showed remarkable antifungal activities against several fungi including Aspergillus niger, Botrytis cinerea causing the gray mold disease, and Candida albicans. In field tests using the purified rKRs-AFP protein, the protein showed the reducing activity of disease spot and the mitigating effect of spreading of disease like agrichemicals. The immuno-assay of rKRs-AFP protein showed that the purified protein entirely accumulated at B. cinerea cytoplasm through the hyphal septa shown by fluorescence imaging. There was no fluorescence inside the cell, when the hypha was incubated without the protein. These all results indicate that the recombinant rKRs-AFP proteins can be utilized as a potential antifungal drug to control harmful plant fungal pathogens.

• 한국통신학회논문지
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• 제31권9C호
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• pp.817-824
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• 2006

본 논문은 이진(binary) 상보형 수열 쌍(complementary pair)을 대역확산 부호로 사용하여 16-QAM 변조된 2개의 심볼을 동시에 전송하는 MC-CDMA(Multi-Carrier Code Division Multiple Access) 신호의 전력 포락선 특성 분석에 대한 것이다. 전력 포락선 분석을 통해 그 특성을 결정짓는 16-QAM 심볼 조합의 관계를 파라미터로 도출하고, 256개의 가능한 전송 심볼 조합 가운데 오직 60개의 서로 다른 전력 포락선이 존재함을 보였다. 또한 복소 평면에서 직관적 등가 변환 관계를 고찰하고 60개의 전력 포락선이 결국 16개의 서로 다른 전력 포락선 변이도(crest factor)를 유발함을 확인하였다. 동일한 변조 기법을 채택한 단일 반송파 신호와 비교하기 위하여 전력 포락선 변이도를 두 전송 심볼의 조합에 의해 발생하는 평균 전력에 따라 분류하였을 때, 전송 심볼의 모든 조합에 대하여 전력 포락선의 변이도가 3dB 이하임을 증명하였다.

• 대한임상검사과학회지
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• 제43권2호
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• pp.68-74
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• 2011

Pseudomonas aeruginosa is an aerobic, Gram-negative, glucose-nonfermenting bacterium, which has emerged as a serious opportunistic pathogen. Recently, outbreaks of carbapenem resistant P. aeruginosa give rise to significant therapeutic challenges for treating nosocomial infections. The genes of metallo-${\beta}$-lactamase (MBL), a powerful carbapenemase, are carried as a part of the mobile gene cassettes inserted into integrons playing an important role in rapid dissemination of antibiotic resistance genes among bacterial isolates. In this study, we investigated the prevalence of integron in imipenem resistant P. aeruginosa isolates. A total of 61 consecutive, non-duplicate, and imipenem resistant P. aeruginosa strains were isolated from a university hospital in the Chungcheong province of Korea. We employed repetitive extragenic palindromic sequence-based PCR (rep-PCR) method for the selection of clonally different P. aerusinosa strains. PCR and DNA sequencing were conducted for the detection of integrons. Twenty-one clonally different P. aeruginosa strains were isolated. Only one (P28) of the strains harbored $bla_{VIM-2}$ that was found as gene cassettes in class 1 integrons. Four of 21 carbapenem resistant P. aeruginosa strains harbored class 1 integron containing aminoglycoside resistance determinant. All of the integrons detected in the study contained more than one resistance gene cassette, which can mediate resistance to multiple antibiotics. To prevent further spreading of the multi-drug resistant P. aeruginosa, conseguent monitoring and clinical polices are required.

• 한국통신학회논문지
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• 제29권6A호
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• pp.631-639
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• 2004

본 논문에서는 다중전송속도를 갖는 DS-CDMA (direct sequence code division multiple access) 하향링크에서 코드의 그룹화와 그룹단위의 역상관을 이용하는 새로운 다중사용자 간섭 억압기법을 제시한다. 다중전송속도를 지원하기 위해 사용되는 OVSF (orthogonal variable spreading factor) 코드에서 모코드와 자코드 간의 상관관계를 이용하여 코드그룹화가 수행된다. 그룹단위의 역상관을 이용하여 제안된 수신기는 기존에 제안된 다른 수신기들보다 비교적 적은 연산복잡도를 가지며, 간섭을 일으키는 다른 사용자에 대한 정보(코드, 데이터, 진폭 등)가 요구되지 않는다. 컴퓨터 모의실험을 통하여 제안된 수신기는 다중경로 채널 환경에서 기존의 간섭제거방식의 수신기보다 월등한 성능향상을 제공함을 알 수 있었다.

• 대한수의학회지
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• 제43권1호
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• pp.95-102
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• 2003

Because Staphylococcus aureus (S. aureus) has variable number of short sequence repeat region in coagulase gene, it has been used to investigate the relatedness of S. aureus isolates. In this study, we isolated S. aureus strains from 20 dairy farms with bovine mastitis from September 2000 to August 2001. PCR-RFLP analysis of coagulase gene revealed 10 different patterns. Most of the S. aureus isolates showed only one coagulase gene RFLP pattern per farm. However, there were several S. aureus clones spreading between dairy farms. All the farms showed poor management conditions of milking machine and milker, indicating that managements for mastitis control program include use of proper milking matching, premilking sanitation, and segregation in the S. aureus infection herd. Our data suggest that PCR-RFLP analysis of coagulase gene might be applicable for the epidemiological investigations of S. aureus isolated from bovine mastitis cows.

• IEIE Transactions on Smart Processing and Computing
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• 제3권4호
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• pp.181-190
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• 2014

This paper reports a cognitive radio network architecture based on overloaded multicarrier direct sequence code division multiple access (O-MC-DS-CDMA). The O-MC-DSCDMA technique combines CDMA with a multicarrier modulation technique to overcome the channel fading effects. In this technique, secondary users are enabled to share the available bandwidth with the existing primary users. Two sets of orthogonal Gold codes are used to support the primary and secondary users simultaneously. The orthogonality between the spreading codes is lost due to the non-zero cross correlation between the codes and the timing synchronization error in the uplink transmission, which causes interference between primary and secondary users. This paper proposes two modified hybrid parallel/successive interference cancellation techniques for primary and secondary user base station receivers with multiple antennas to suppress the interference among users. Interference among the same group of users is cancelled by parallel interference cancellation and the interference among groups is cancelled using successive interference cancellation. The simulation results confirmed that the proposed modified interference cancellation techniques show better BER performance over conventional interference cancellation techniques.