• Journal of Korean Society of Forest Science
• /
• v.96 no.1
• /
• pp.7-13
• /
• 2007

This study was done to develop PDA based application system for forest geographic information with GPS. The major results obtained in this study were as follows. A PDA based application program was developed to work on $Microsoft^{TM}$ PocketPC 2002 and 2003 operating system. The screen of PDA displays a 1:25,000 digital topographical map adopted DXF format converted from PC, and the map data with 1:2,500 to 1:30,000 on PDA can be zoomed in or out to five levels. Current position and navigating path received from GPS can be displayed on the screen and be saved in PDA. Information selected among layers of digital topographic map in DXF format can be converted into binary files which can be used on forest geographic information software. This can compress DXF files to 90% in size, and the processing speed of PDA was improved. The forest geographic information management system can be used to manage sample plots on which forest inventory is done, with the help of the sub-menus and grid index values with position information received from GPS. Forest workers can in quire forest geographic information such as forest type, location, forest roads, soil erosion control dams using forest geographic information management system in the field. The forest geographic information management system can provide current position and mobile path information to people who enjoy forest related activities like mountain-climbing, sightseeing, and visiting to historic spots.

• Genomics & Informatics
• /
• v.6 no.1
• /
• pp.18-28
• /
• 2008

Single nucleotide polymorphisms (SNPs) are the most abundant forms of human genetic variations and resources for mapping complex genetic traits and disease association studies. We have constructed a linkage disequilibrium (LD) map of chromosome 22 in Korean samples and compared it with those of other populations, including Yorubans in Ibadan, Nigeria (YRI), Centre d'Etude du Polymorphisme Humain (CEPH) reference families (CEU), Japanese in Tokyo (JPT) and Han Chinese in Beijing (CHB) in the HapMap database. We genotyped 4681 of 111,448 publicly available SNPs in 90 unrelated Koreans. Among genotyped SNPs, 4167 were polymorphic. Three hundred and five LD blocks were constructed to make up 18.6% (6.4 of 34.5 Mb) of chromosome 22 with 757 tagSNPs and 815 haplotypes (frequency $\geq$ 5.0%). Of 3430 common SNPs genotyped in all five populations, 514 were monomorphic in Koreans. The CHB + JPT samples have more than a 72% overlap with the monomorphic SNPs in Koreans, while the CEU + YRI samples have less than a 38% overlap. The patterns of hot spots and LD blocks were dispersed throughout chromosome 22, with some common blocks among populations, highly concordant between the three Asian samples. Analysis of the distribution of chimpanzee-derived allele frequency (DAF), a measure of genetic differentiation, Fst levels, and allele frequency difference (AFD) among Koreans and the HapMap samples showed a strong correlation between the Asians, while the CEU and YRI samples showed a very weak correlation with Korean samples. Relative distance as a quantitative measurement based upon DAF, Fst, and AFD indicated that all three Asian samples are very proximate, while CEU and YRI are significantly remote from the Asian samples. Comparative genome-wide LD studies provide useful information on the association studies of complex diseases.

• Asian-Australasian Journal of Animal Sciences
• /
• v.25 no.11
• /
• pp.1540-1545
• /
• 2012

Steroidogenesis requires coordination of the anabolic and catabolic pathways of lipid metabolism, but the profile of proteins associated with progesterone synthesis in cyclic and pregnant corpus luteum (CL) is not well-known in cattle. In Experiment 1, plasma progesterone level was monitored in cyclic cows (n = 5) and pregnant cows (n = 6; until d-90). A significant decline in the plasma progesterone level occurred at d-19 of cyclic cows. Progesterone level in abbatoir-derived luteal tissues was also determined at d 1 to 5, 6 to 13 and 14 to 20 of cyclic cows, and d-60 and -90 of pregnant cows (n = 5 each). Progesterone level in d-60 CL was not different from those in d 6 to 13 CL and d-90 CL, although the difference between d 6 to 13 and d-90 was significant. In Experiment 2, protein expression pattern in CL at d-90 (n = 4) was compared with that in CL of cyclic cows at d 6 to 13 (n = 5). Significant changes in the level of protein expression were detected in 32 protein spots by two-dimensional polyacrylamide gel electrophoresis (2-DE), and 23 of them were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Six proteins were found only in pregnant CL, while the other 17 proteins were found only in cyclic CL. Among the above 6 proteins, vimentin which is involved in the regulation of post-implantation development was included. Thus, the protein expression pattern in CL was disorientated from cyclic luteal phase to mid pregnancy, and alterations in specific CL protein expression may contribute to the maintenance of pregnancy in Korean native cows.

• Asian Pacific Journal of Cancer Prevention
• /
• v.17 no.1
• /
• pp.197-200
• /
• 2016

Background: Wedge filters are commonly used in radiation oncology for eliminating hot spots and creating a uniform dose distribution in optimizing isodose curves in the target volume for clinical aspects. These are some limited standard physical wedges ($15^{\circ}$, $30^{\circ}$, $45^{\circ}$, $60^{\circ}$),or creating an arbitrary wedge angle, like motorized wedge or dynamic wedge,${\ldots}$ The new formulation is presented by the combination of wedge fields for determining an arbitrary effective wedge angles. The isodose curves also are derived for these wedges. Materials and Methods: we performed the dosimetry of Varian Clinac 2100C/D with Scanditronix Wellhofer water blue phantom, CU500E, OmniPro - Accept software and 0.13cc ionization chamber for 6Mv photon beam in depth of 10cm (reference depth) for universal physical wedges ($15^{\circ}$, $30^{\circ}$, $45^{\circ}$, and $60^{\circ}$) and reference field $10.10cm^2$. By combining the isodose curve standard wedge fields with compatible weighting dose for each field, the effective isodose curve is calculated for any wedge angle. Results: The relation between a given effective wedge angle and the weighting of each combining wedge fields was derived. A good agreement was found between the measured and calculated wedge angles and the maximum deviation did not exceed $3^{\circ}$. The difference between the measured and calculated data decreased when the combined wedge angles were closer. The results are in agreement with the motorized single wedge appliance in the literature. Conclusions: This technique showed that the effective wedge angle that is obtained from this method is adequate for clinical applications and the motorized wedge formalism is a special case of this consideration.

• Asian Pacific Journal of Cancer Prevention
• /
• v.15 no.7
• /
• pp.3311-3317
• /
• 2014

Background: The consequence of Rho GDP dissociation inhibitor alpha (RhoGDI${\alpha}$) activity on migration and invasion of estrogen receptor positive ($ER^+$) and negative ($ER^-$) breast cancer cells has not been studied using the proteomic approach. Changes in expression of RhoGDI${\alpha}$ and other proteins interacting directly or indirectly with RhoGDI${\alpha}$ in MCF7 and MDA-MB-231, with different metastatic potentials is of particular interest. Materials and Methods: $ER^+$ MCF7 and ER- MDA-MB-231 cell lines were subjected to two-dimensional electrophoresis (2-DE) and spots of interest were identified by matrix-assisted laser desorption/ionization time of- flight/time-of-flight (MALDI-TOF/TOF) mass spectrometry (MS) analysis after downregulation of RhoGDI${\alpha}$ using short interfering RNA (siRNA) and upregulated using GFP-tagged ORF clone of RhoGDI${\alpha}$. Results: The results showed a total of 35 proteins that were either up- or down-regulated in these cells. Here we identifed 9 and 15 proteins differentially expressed with silencing of RhoGDI${\alpha}$ in MCF-7 and the MDA-MB-231 cells, respectively. In addition, 10 proteins were differentially expressed in the upregulation of RhoGDI${\alpha}$ in MCF7, while only one protein was identified in the upregulation of RhoGDI${\alpha}$ in MDA-MB-231. Based on the biological functions of these proteins, the results revealed that proteins involved in cell migration are more strongly altered with RhoGDI-${\alpha}$ activity. Although several of these proteins have been previously indicated in tumorigenesis and invasiveness of breast cancer cells, some ohave not been previously reported to be involved in breast cancer migration. Hence, these proteins may serve as useful candidate biomarkers for tumorigenesis and invasiveness of breast cancer cells. Conclusions: Future studies are needed to determine the mechanisms by which these proteins regulate cell migration. The combination of RhoGDI${\alpha}$ with other potential biomarkers may be a more promising approach in the inhibition of breast cancer cell migration.

• Journal of Korean Tunnelling and Underground Space Association
• /
• v.17 no.2
• /
• pp.127-140
• /
• 2015

A fault is one of the critical factors that may lead to a possible ground collapse occurring in construction site. A fault core, however, possibly acting as a failure plane in whole fault zone, is composed of fractured rock and gouge nonuniformly distributed and thus can be characterized by its wide range of shear strength which is generally acquired by experimental method for stability analysis. In this study, we performed direct shear test and grain size distribution analysis for 62 fault core samples cropped from 12 different spots located in the vicinity of Kyongju and Ulsan, Korea. As a result, the range of shear strength representing the characteristics of fault cores in the study regions is determined with regard to vertical stress using a regression analysis for experiment data. The weight ratio of gravels in the samples is proportional to the shear strength and that of silt and clay is in inverse proportion to the shear strength. For most samples, the coefficient of determination is over 0.7 despite of inhomogeneity of them and consequently we determined the lower limit and upper limit of the shear strength with regard to the weight ratio by setting the confidence interval of 95%.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.9 no.4
• /
• pp.273-280
• /
• 1976

Morphological changes of early larval stages of Lophius litulon (Jordan) are described based on 861 individuals collected at the intertidal zone at Haeundae, Busan, on the 20th of June, 1976. Particular emphasis is paid on the development of dorsal spinous rays, ventral fin rays, chromatophore patterns, alimentary canal, gills, branchiostegal rays and teeth. The premordial spinous fin ray of the first dorsal fin appears at the newly hatched larva of around 2.70mm in total length, the second spinous ray at around 5.82mm, and the third spinous ray at around 6.45 nm. Premordial fin rays which develope as a semicircle to cudgel form of the ventral fin appear posterior to the pectoral fin at the larva of around 2.70mm in total length. When the larvae grow up to 6.35mm in total length, the length of the ventral fin is more or less half of the total length of the body. In the early stages$(2.70\~3.75mm)$ melanophores are scattered irregularly on the tail . In the later stages(4.25mm) melanophores are aggregated to form three black spots on medio-lateral part of the tail. The newly hatched larvae have the premordial alimentary canal with mouth and intestine. With decreasing yolk mass the intestine appears more distinctly with curvature, and mouth seems to open. Gills appear at the larvae of 6.35mm in total length; branchiostegal rays appear at the larvae of 6.45mm. Teeth appear at the larvae of 6.25mm.

• The Journal of Korean Medicine
• /
• v.28 no.2 s.70
• /
• pp.93-113
• /
• 2007

Objective : To research Trends of studies about anti-inflammation and pain relief, obesity, and safety of herbal acupuncture therapy by analyzing domestic theses, published since 2001, about herbal acupuncture therapy. Methods : Domestic theses, published since 2001, mentioning anti-inflammation or pain relief, obesity, or safety of herbal acupuncture therapy were reviewed and analyzed. These theses were then classified by university, year, and subject. Results : The following results were obtained in this study. 1. Among published theses related to anti-inflammation effects of herbal-acupuncture, studies about arthritis comprised the most part, 52. In theses relating to causes of arthritis, 16 were about adjuvant, which was the most, followed by Type II collagen, LPS and carrageenan. Blood test, reactions of inflammation and revelation of cytokine and immune cellswere methods for evaluating anti-inflammation effect. The tendency of experimental methods was to focus on molecular biologic method. 2. In theses related to pain relief, many clinical attempts with herbal injection were carried out, and Carthami Flos and Scolopendrawere used most. Observing reduction of pain inducing factor and checking behavioral change were methods for evaluating pain relief. 3. In theses related to obesity, research focused on effects in association with spots on the body suitable for acupuncture. There were also attempts comparing effectiveness between single injections and complex injections. Astraball Radix, Angelica Gigantis Radix, Coicis Semen and Taeumjowetang were used. Evaluation of anti-obesity effects were by weight loss, food efficiency, blood lipid profile and evaluation of liver function. 4. In theses related to safety of herbal-acupuncture, Herba Chelidonii Chaenonelis Fructus, Clematis Florida Thunb, Corydalidis Tuber, Paeoniae Radix, and Carthami Flos which marked 2 theses each were most studied. Methods of evaluating safety were mostly by observing liver and kidney functions based on blood test, and by applying herbal injections to clinical treatment. Conclusion : Herbal acupuncture is being used in various ways associating with its anti-inflammation, pain relief and anti-obesity effect. Studies on efficacy and mechanism of herbal acupuncture are being conducted even at the molecular biology level.

• Journal of Korean Society for Atmospheric Environment
• /
• v.23 no.5
• /
• pp.596-611
• /
• 2007

Resuspended dust from paved roads in Seoul and Incheon metropolitan areas is regarded as one of the major $PM_{10}$ sources in these areas, according to the recent emission estimates using the emission factors compiled in AP-42. It is well known that the AP-42 model for estimating $PM_{10}$ emissions from paved roads requires information on silt loadings of particular paved roads. The conventional AP-42 method (vacuum swept method) for road silt sampling, however, is expensive, time consuming, and dangerous. These drawbacks led us to develop a Mobile Dust Monitoring System (MDMS) capable of doing real time measurements of silt loading of paved roads, thereby we could get higher resolution silt loading data both in terms of time and space without too much human efforts and danger. In this study, for the real-time measurement of silt loading of paved roads, the principle used in the TRAKER method of U.S. Desert Research Institute was employed and the entire sampling systems including data acquisition system were designed for theses purpose and mounted on a SUV. The correlation between the silt loading measured by vacuum swept method and the speed corrected ${\Delta}Dust$ was derived for the vehicle-based silt loading measurements, and then the variations of silt loading on paved roads were surveyed using the MDMS in test routes of Seoul and Incheon. From the results of real-time measurements, temporal and spatial variations of silt loading data together with the existence of hot spots were observed for paved roads in Seoul and Incheon. The result of this study will be employed to estimate fugitive dust emissions from paved roads.

• Journal of Korean Society of Forest Science
• /
• v.87 no.3
• /
• pp.334-340
• /
• 1998

This research was conducted to identify plant regulatory regions by gene tagging method. A promoterless GUS coding sequence was introduced to Populus nigra ${\times}$ P. maximowiczii via Agrobacterium strains(LBA4404/EHA101), and putative transgenic poplars were selected by culturing on medium containing G418($60mg/{\ell}$) and by GUS assay. Among them one positive plant was to amplify the native sequences flanking to the introduced GUS gene in plant genome by inverse PCR method and from this 730 by DNA product was obtained. After subcloning and sequencing, it has 88% homology to the Eucalyptus gunnii CAD(cinnamyl alcohol dehydrogenase) gene. The GUS gene fused with the putative promoter reinserted into poplar leaves by particle bombardment method to test the funtional promoter activity. Upon staining with X-gluc, many blue spots appeared on the leaf segments bombarded by the chimeric gene 2-3 days, thus the isolated DNA fragment contain some possible coding region as well as a putative regulatory sequences of poplar CAD gene.