• 제목/요약/키워드: spot-uniformity

검색결과 39건 처리시간 0.024초

Quality Control Probes for Spot-Uniformity and Quantitative Analysis of Oligonucleotide Array

  • Jang, Hyun-Jung;Cho, Mong;Kim, Hyung-Hoi;Kim, Cheol-Min;Park, Hee-Kyung
    • Journal of Microbiology and Biotechnology
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    • 제19권7호
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    • pp.658-665
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    • 2009
  • Quality control QC for spot-uniformity is a critical point in fabricating an oligonucleotide array, and quantification of targets is very important in array analysis. We developed two new types of QC probes as a means of confirming the quality of the uniformity of attached probes and the quantification of targets. We compared the signal intensities and fluorescent images of the QC and target-specific probes of arrays containing only target-specific probes and those containing both QC and target-specific probes. In a comparison of quality control methods, it was found that the arrays containing QC probes could check spot-uniformity or spot defects during all processes of array fabrication, including after spotting, after washing, and after hybridization. In a comparison of quantification results, the array fabricated by the method using QC probes showed linear and regular results because it was possible to normalize variations in spot size and morphology and amount of attached probe. This method could avoid errors originating in probe concentration and spot morphology because it could be normalized by QC probes. There were significant differences in the signal intensities of all mixtures (P<0.05). This result indicates that the method using QC probes is more useful than the ordinary method for quantification of mixed target. In the quantification of mixed targets, this method could determine a range for mixed targets of various amounts. Our results suggest that methods using QC probes for array fabrication are very useful to the quality control of spots in the fabrication processes of quantitative oligonucleotide arrays.

Hot spot 감소를 위한 Edge Type LED Backlight 설계 (Design of Edge Type LED Backlight System for reduce Hot spot)

  • 김용숙;임성규
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2004년도 추계학술대회 논문집 Vol.17
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    • pp.551-554
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    • 2004
  • LED는 소비전력이 적고, 수명이 길어 LCD Backlight 용 광원으로 주목받고 있다. 현재 휴대전화기용 LCD에 많이 사용되고 있는 소형 LED Backlight의 경우 입광부에 LED의 개수가 육안으로 확인 가능할 정도의 Hot Spot이 존재한다. 이에 본 논문에서는 Reflector를 사용하여 광의 손실을 줄이고 도광판의 입광부에 산란 패턴을 설계하여 적용함으로써 도광판 입광부에서 122.7%의 휘도 상승효과와 73.4%의 휘도 균일도를 얻을 수 있었으며 이로 인해 Hot Spot을 감소시킬 수 있었다.

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타이어 플랫 스팟에 의한 차량진동 연구 (Vehicle Vibration Study by Tire Flat Spot)

  • 박주표;최정현;이상주
    • 한국소음진동공학회:학술대회논문집
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    • 한국소음진동공학회 2007년도 추계학술대회논문집
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    • pp.1395-1400
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    • 2007
  • Tire flat spot is a deformation which occurs around the contact patch during long-period parking and does hardly recovered even after driving. The deformation makes a tire self-excited and ride comfort gets worse. In this study, it is shown that the flat spot can be evaluated by measuring change in radial run out or force. Its effects on vibration at vehicle floor and steering wheel are also revealed. Finally it is shown that the flat spot is likely to occur if the inflation pressure is low and the tire is suppressed by a heavy load at a high temperature.

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5 kW급 고온형 연료전지 촉매 연소기 유동 균일화 장치가 연소 특성에 미치는 영향 (Effect of Flow Uniformity Device on the Catalytic Combustor for 5 kW High Temperature Fuel Cell System)

  • 이상민;우현탁;안국영
    • 한국수소및신에너지학회논문집
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    • 제22권6호
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    • pp.878-883
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    • 2011
  • Effect of flow uniformity on the reaction characteristics of a catalytic combustor for high temperature fuel cell system has been experimentally investigated in the present study. One of the most important factor in designing catalytic combustion is to avoid hot spot in catalysts. In this regard, it is very important to secure flow uniformity of combustor inlet. A couple of perforated plates were applied at the front of catalyst region as flow uniformity device with minimal pressure drop. Results show that the velocity and temperature profile became more uniform when applying the flow uniformity device. CO and $CH_4$ emissions at the combustor exit were decreased and the average exit temperature was slightly increased with the flow uniformity device.

Analysis of Specific Problems in Laser Scanning Optical System Design

  • Joo, Won-Don
    • Journal of the Optical Society of Korea
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    • 제15권1호
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    • pp.22-29
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    • 2011
  • We analyze aberrations in an optical laser printer system in order to know how to determine an allowable non-uniformity of the movement of a light spot, how to determine allowed variation of spot sizes, and how to minimize the influence of these deviations on technological errors. In this paper, the correction and the tolerance of distortion are analyzed by using the concept of zonal and global distortions. The tolerance of field curvature is also obtained from Gaussian beam properties. In order to reduce the change of the entrance pupil position and to make a more compact laser printer system the minimum size of the rotator is exactly derived from the geometry with the introduction of the shift angle of the input beam.

MCFC Off-gas 촉매연소기 설계에 관한 연구 (A Study on the Design of MCFC Off-Gas Catalytic Combustor)

  • 이상민;이영덕;안국영;홍동진;김만영
    • 한국수소및신에너지학회논문집
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    • 제18권4호
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    • pp.406-412
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    • 2007
  • An experimental study on the design of a catalytic combustor for 1.6 kW MCFC system has been performed. The roles of the catalytic combustor are to completely burn anode off-gas and to supply sufficient $CO_2$ to cathode channels. In order to avoid hot spot or fuel slippage, flow uniformity at the catalyst inlet was achieved by installing two crossing perforated plates between the catalyst and the mixing chamber with minimal pressure drop. A Pd/Ce/Ni-$Al_2O_3$ catalyst was used for complete combustion of the off-gas at GHSV=36,000.

Optimization of Diffractive Optical Elements by Genetic Algorithm

  • Yoon, Jin-Seon;Kim, Nam
    • Journal of the Optical Society of Korea
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    • 제4권1호
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    • pp.30-36
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    • 2000
  • In this paper, a method based on the Genetic Algorithm(GA) for phase optimization is proposed. The programmable hybrid optical interconnection system implemented by the spatial light modula-tor is tested for a near-real-time optical processing. Designed diffractive grating has a 74.7[%] high diffraction efficiency and a $1.73 {\times}10^{-1}$ uniformity quantitatively. The dependence of characteris-tics on several parameters in the grating design are analyzed. Also, as a result of the geometrical transformation to obtain quantitative data for $3 {\times} 3$ spot beams, an objective optical experiment using CCD array detector produces the mean of beam intensity as a gray level of 202, the maximum value is 225, the minimum value is 186, and uniformity is quantitatively $1.93 {\times} 10^{-1}$, similar to the simulation result.

LCD용 컬러필터 보호막 (Thin layer(Overcoat) for TFT-LCD color filter)

  • 김명구;박주현;임영택
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2006년도 추계학술대회 논문집 Vol.19
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    • pp.273-273
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    • 2006
  • Over the past years, a large number of acrylate polymers have been developed and the overcoat thin layer containing acrylate polymers have been used for TFT-LCD color filter. As forming thin layer using acrylate polymers, the existing acrylate polymers have some problems such as low hardness by low Tg temperature, coating uniformity and solubility in organic solvent. To solve these problems, we synthesized new polymer(Scheme.), containing olefin monomer, which has high Tg temperature, good coating uniformity and good solubility in organic solvent. The overcoat thin layer containing new polymer resulted in good coating uniformity, stain, spot, scratch, heat resistance, DOP(Degree Of Planarization) on RGB glass, transparency, hardness, adhesion, anti-chemicals(anti-acid, anti-base, anti-organic solvent), insulation and anti-humidity. Scheme. The structure of new polymer X = Olefin monomer contains ketone, ester, hydroxy, ether, halogen, nitrile, alkoxy, phenyl functional group $R_1$ and $R_2$= H or $CH_3$. Ratio=0<[1/(1+m+n)]<0.7,0.1[$\leq$[n/(1+m+n)]<0.5.

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MTFA에 의한 칼라 CRT의 화질 평가 (Evaluation of color CRT monitor by MTFA)

  • 김태희
    • 한국광학회지
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    • 제9권3호
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    • pp.205-211
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    • 1998
  • 칼라 CRT(cathode ray tube)의 화질을 평가하기 위하여 일차원 CCD(charge coupled-device)를 사용한 MTF(modulation transfer function)측정장치를 구성하였다. CCD의 분광감도와 화소의 크기와 간격을 고려하여 MTF 측정값을 보정하였다. Spot 크기, 비디오 대역폭, 새도우 마스크, 디스플레이 휘도, 그리고 주변 조명이 디스플레이의 미치는 영향에 대하여 연구하였다. 칼라 CRT 모니터에서 화면의 균일성과 주변 조명에 따른 화질 평가하였으며, 그 결과를 MTFA(modulation transfer function area)를 사용하여 나타내었다.

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가스터빈용 촉매 연소기의 유동 특성에 관한 연구 (A Study on the Flow Characteristics of the Catalytic Combustor for the Gas Turbine)

  • 홍동진;김종민;김만영
    • 한국항공우주학회지
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    • 제35권9호
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    • pp.792-798
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    • 2007
  • 촉매 연소는 통상의 연소 방식과는 달리 촉매 표면에서 연료와 산화제의 화학 반응에 의한 연소 방식이다. 따라서 연소 영역 이전에 연료와 공기가 잘 혼합되어야 하며, 균일한 공급이 이루어져야 한다. 만약 불균일한 유동 분포가 발생하게 되면, 국부적인 고온 지역이 발생하게 되고 이는 촉매와 지지체에 손상을 초래하게 된다. 본 연구에서는 촉매 연소기의 혼합 및 유동 균일도를 향상시키기 위해 다공판을 사용하였으며, 다양한 파라메터의 변화에 따른 유동 특성의 변화를 조사하기 위해 수치적인 방법을 사용하였다. 각각의 조건하에서 촉매부의 입구에서 유동의 균일도를 조사하고 평가하였으며, 그 결과 적절히 설계된 다공판을 사용하였을 경우 대부분의 해석 조건에 대해 효과적인 균일도 향상을 나타냄을 확인할 수 있었다.