• Journal of Microbiology and Biotechnology
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• v.19 no.7
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• pp.658-665
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• 2009

Quality control QC for spot-uniformity is a critical point in fabricating an oligonucleotide array, and quantification of targets is very important in array analysis. We developed two new types of QC probes as a means of confirming the quality of the uniformity of attached probes and the quantification of targets. We compared the signal intensities and fluorescent images of the QC and target-specific probes of arrays containing only target-specific probes and those containing both QC and target-specific probes. In a comparison of quality control methods, it was found that the arrays containing QC probes could check spot-uniformity or spot defects during all processes of array fabrication, including after spotting, after washing, and after hybridization. In a comparison of quantification results, the array fabricated by the method using QC probes showed linear and regular results because it was possible to normalize variations in spot size and morphology and amount of attached probe. This method could avoid errors originating in probe concentration and spot morphology because it could be normalized by QC probes. There were significant differences in the signal intensities of all mixtures (P<0.05). This result indicates that the method using QC probes is more useful than the ordinary method for quantification of mixed target. In the quantification of mixed targets, this method could determine a range for mixed targets of various amounts. Our results suggest that methods using QC probes for array fabrication are very useful to the quality control of spots in the fabrication processes of quantitative oligonucleotide arrays.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2004.11a
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• pp.551-554
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• 2004

LED는 소비전력이 적고, 수명이 길어 LCD Backlight 용 광원으로 주목받고 있다. 현재 휴대전화기용 LCD에 많이 사용되고 있는 소형 LED Backlight의 경우 입광부에 LED의 개수가 육안으로 확인 가능할 정도의 Hot Spot이 존재한다. 이에 본 논문에서는 Reflector를 사용하여 광의 손실을 줄이고 도광판의 입광부에 산란 패턴을 설계하여 적용함으로써 도광판 입광부에서 122.7%의 휘도 상승효과와 73.4%의 휘도 균일도를 얻을 수 있었으며 이로 인해 Hot Spot을 감소시킬 수 있었다.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2007.11a
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• pp.1395-1400
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• 2007

Tire flat spot is a deformation which occurs around the contact patch during long-period parking and does hardly recovered even after driving. The deformation makes a tire self-excited and ride comfort gets worse. In this study, it is shown that the flat spot can be evaluated by measuring change in radial run out or force. Its effects on vibration at vehicle floor and steering wheel are also revealed. Finally it is shown that the flat spot is likely to occur if the inflation pressure is low and the tire is suppressed by a heavy load at a high temperature.

• Transactions of the Korean hydrogen and new energy society
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• v.22 no.6
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• pp.878-883
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• 2011

Effect of flow uniformity on the reaction characteristics of a catalytic combustor for high temperature fuel cell system has been experimentally investigated in the present study. One of the most important factor in designing catalytic combustion is to avoid hot spot in catalysts. In this regard, it is very important to secure flow uniformity of combustor inlet. A couple of perforated plates were applied at the front of catalyst region as flow uniformity device with minimal pressure drop. Results show that the velocity and temperature profile became more uniform when applying the flow uniformity device. CO and $CH_4$ emissions at the combustor exit were decreased and the average exit temperature was slightly increased with the flow uniformity device.

• Journal of the Optical Society of Korea
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• v.15 no.1
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• pp.22-29
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• 2011

We analyze aberrations in an optical laser printer system in order to know how to determine an allowable non-uniformity of the movement of a light spot, how to determine allowed variation of spot sizes, and how to minimize the influence of these deviations on technological errors. In this paper, the correction and the tolerance of distortion are analyzed by using the concept of zonal and global distortions. The tolerance of field curvature is also obtained from Gaussian beam properties. In order to reduce the change of the entrance pupil position and to make a more compact laser printer system the minimum size of the rotator is exactly derived from the geometry with the introduction of the shift angle of the input beam.

• Transactions of the Korean hydrogen and new energy society
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• v.18 no.4
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• pp.406-412
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• 2007

An experimental study on the design of a catalytic combustor for 1.6 kW MCFC system has been performed. The roles of the catalytic combustor are to completely burn anode off-gas and to supply sufficient $CO_2$ to cathode channels. In order to avoid hot spot or fuel slippage, flow uniformity at the catalyst inlet was achieved by installing two crossing perforated plates between the catalyst and the mixing chamber with minimal pressure drop. A Pd/Ce/Ni-$Al_2O_3$ catalyst was used for complete combustion of the off-gas at GHSV=36,000.

• Journal of the Optical Society of Korea
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• v.4 no.1
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• pp.30-36
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• 2000

In this paper, a method based on the Genetic Algorithm(GA) for phase optimization is proposed. The programmable hybrid optical interconnection system implemented by the spatial light modula-tor is tested for a near-real-time optical processing. Designed diffractive grating has a 74.7[%] high diffraction efficiency and a $1.73 {\times}10^{-1}$ uniformity quantitatively. The dependence of characteris-tics on several parameters in the grating design are analyzed. Also, as a result of the geometrical transformation to obtain quantitative data for $3 {\times} 3$ spot beams, an objective optical experiment using CCD array detector produces the mean of beam intensity as a gray level of 202, the maximum value is 225, the minimum value is 186, and uniformity is quantitatively $1.93 {\times} 10^{-1}$, similar to the simulation result.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2006.11a
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• pp.273-273
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• 2006

Over the past years, a large number of acrylate polymers have been developed and the overcoat thin layer containing acrylate polymers have been used for TFT-LCD color filter. As forming thin layer using acrylate polymers, the existing acrylate polymers have some problems such as low hardness by low Tg temperature, coating uniformity and solubility in organic solvent. To solve these problems, we synthesized new polymer(Scheme.), containing olefin monomer, which has high Tg temperature, good coating uniformity and good solubility in organic solvent. The overcoat thin layer containing new polymer resulted in good coating uniformity, stain, spot, scratch, heat resistance, DOP(Degree Of Planarization) on RGB glass, transparency, hardness, adhesion, anti-chemicals(anti-acid, anti-base, anti-organic solvent), insulation and anti-humidity. Scheme. The structure of new polymer X = Olefin monomer contains ketone, ester, hydroxy, ether, halogen, nitrile, alkoxy, phenyl functional group $R_1$ and $R_2$= H or $CH_3$. Ratio=0<[1/(1+m+n)]<0.7,0.1[$\leq$[n/(1+m+n)]<0.5.

• Korean Journal of Optics and Photonics
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• v.9 no.3
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• pp.205-211
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• 1998

The MTF(modulation transfer function) measuring system with a linear CCD(charge coupled-device) was constructed to cvaluate a color CRT(catode ray tube). The measured MTF values were corrected by considering the spectral response and the pixel sizes of CCD. The effects of a spot size, video bandwidth, pitch of shadow mask holes, display luminance, and ambient illumination on image quality were studied. The uniformity of resolution and the contrast Ioss by ambient light of the color CRT monitor were measured, and the results were analyzed by MTFA(modulation threshold area).

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.35 no.9
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• pp.792-798
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• 2007

catalytic combustion is accomplished by the chemical reaction between fuel and oxidizer at the catalyst surface, different from conventional combustion. Therefore, it is important that the fuel and air stream are well mixed and supplied uniformly prior to the combustion region. If the flow is maldistributed, a hot spot may occur that can lead to subsequent catalyst and substrate damage. Therefore, in order to enhance the mixing and flow uniformity, in this study, the perforated plate is used. A numerical simulation is performed to investigate the variation of flow characteristics by changing various parameters. Under each condition, the uniformity of the flow stream at the entrance of the catalyst section is evaluated and compared. The results show that the uniformity can be effectively improved for most of the case by using the well-designed perforated plates.