• Analytical Science and Technology
• /
• v.32 no.4
• /
• pp.155-162
• /
• 2019

Analysis techniques using DNA profiling are widely used in various fields including forensic science and new technologies such as the Direct PCR amplification method are being developed continuously in order to acquire the DNA profiles efficiently. However, it has a limits such as non-specific amplification according to the quality of crime scene evidence samples. Especially, split peaks caused by excessive DNA samples are one of the important factors that could cause the debate to allow researchers to interpret the DNA profile results. In this study, we confirmed the occurrence rate of split peaks in each STR (short tandem repeats) locus of the $GlobalFiler^{TM}$ kit and investigated the possibility of improving the split peaks using several PCR additives such as DMSO (dimethylsulfoxide), $MgCl_2$, Betaine and Tween-20. As a result, we could make three groups according to the occurrence rate of split peaks in Direct PCR and it was confirmed that the ratio of split peaks could be reduced by DMSO (87.4 %), $MgCl_2$ (84.5 %) and Betaine (86.1 %), respectively. These results indicate that PCR additives such as DMSO, $MgCl_2$ and Betaine can be improve the split peaks in Direct PCR and thereby facilitate subsequently a successful DNA profile results.

• Proceedings of the KIEE Conference
• /
• 2004.07e
• /
• pp.55-57
• /
• 2004

$HgGa_{2}S_{4}Co^{2+}$ single crystal were grown by the chemical transport reaction(CTR) method. In the optical absorption spectrum of the $HgGa_{2}S_{4}Co^{2+}$ single crystal measured at 298K, three groups of impurity optical absorption peaks consisting of three peaks, respectively, were observed at 673nm, 734nm, and 760nm, 1621nm, 1654nm, and 1734nm, and 2544nm, 2650nm, and 2678nm. At 10K, the three peaks(673nm, 734nm, and 760nm) of the first group were split to be twelve peaks. These impurity optical absorption peaks are assigned to be due to the electronic transitions between the split energy levels of $Co^{2+}$ sited in the $S_4$ symmetry point.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
• /
• 2004.05c
• /
• pp.3-7
• /
• 2004

$HgGa_2S_4:CO^{2+}$ single crystal were grown by the chemical transport reaction(CTR) method. 1n the optical absorption spectrum of the $HgGa_2S_4:CO^{2+}$ single crystal measured at 298K, three groups of impurity optical absorption peaks consisting of three peaks, respectively, were observed at 673nm, 734nm, and 760nm, 1621nm, 1654nm, and 1734nm, and 2544nm, 2650nm, and 2678nm. At 10K, the three peaks(673nm, 734nm, and 760nm) of the first group were split to be twelve peaks. These impurity optical absorption peaks are assigned to be due to the electronic transitions between the split energy levels of $Co^{2+}$ sited in the $S_4$ symmetry point.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
• /
• v.16 no.7
• /
• pp.579-583
• /
• 2003

HgGa$_2$S$_4$: Co$^{2＋}$ single crystal were grown by the chemical transport reaction(CTR) method. In the optical absorption spectrum of the HgGa$_2$S$_4$: Co$^{2＋}$ single crystal measured at 298K, three groups of impurity optical absorption peaks consisting of three peaks, respectively, were observed at 673nm, 734nm, and 760nm, 1621nm, 1654nm, and 1734nm, and 2544nm, 2650nm, and 2678nm. At 10K, the three peaks(673nm, 734nm, and 760nm) of the first group were split to be twelve peaks. These impurity optical absolution peaks are assigned to be due to the electronic transitions between the split energy levels of Co$^{2＋}$ sited in the S$_4$ symmetry point.

• Proceedings of the Korean Society For Composite Materials Conference
• /
• 2003.10a
• /
• pp.141-145
• /
• 2003

The FBG sensors are inserted on the liners of the filament wound pressure tanks. The strains near the welding region of the liners are monitored in the hydro-pressurizing tests. The hydro-pressurizing tests consist of the proof tests at 4500 or 3300 psi and repeated test at the operating pressure, 3000 psi. The FBG sensors work well under $3000\mu\varepsilon$, but the strains calculated from the reflected signals are instable at the high strain level. The transverse compression on the sensor head results in the split of the reflected peaks, and the calculating algorism from the split peaks is not robust under the various signal condition. The FBG sensors fracture near $7500\mu\varepsilon$ level and lose their function permanently.

• Journal of Gastric Cancer
• /
• v.3 no.3
• /
• pp.151-157
• /
• 2003

Purpose: In this study, we attempted to ascertain the proton magnetic resonance spectroscopy (${1}^H$ MRS) peak characteristics of human gastric tissue layers and finally to use the metabolic peaks of MRS to distinguish between normal and abnormal gastric specimens. Materials and Methods: Ex-vivo ${1}^H$ MRS examinations of thirty-five gastric specimens were performed to distinguish abnormal gastric tissues invaded by carcinoma cells from normal stomach-wall tissues. High-resolution 400-MHz (9.4-T) ${1}^H$ nuclear magnetic resonance (NMR) spectra of two gastric layers, a proper muscle layer, and a composite mucosasubmucosa layer were compared with those of clinical 64- MHz (1.5-T) MR spectra. Three-dimensional spoiled gradient recalled (SPGR) images were used to determine the size and the position of a voxel for MRS data collection. Results: For normal gastric tissue layers, the metabolite peaks of 400-MHz ${1}^H$ MRS were primarily found to be as follows: lipids at 0.9 ppm and 1.3 ppm; alanine at 1.58 ppm; N-acetyl neuraminic acid (sialic acid) at 2.03 ppm; and glutathione at 2.25 ppm in common. The broad and featureless featureless spectral peaks of the 64-MHz MRS were bunched near 0.9, 1.3, and 2.0, and 2.2 ppm in human specimens without respect to layers. In a specimen (Borrmmann type III) with a tubular adenocarcinoma, the resonance peaks were measured at 1.26, 1.36 and 3.22 ppm. All the peak intensities of the spectrum of the normal gastric tissue were reduced, but for gastric tumor tissue layers, the lactate peak split into 1.26 and 1.39 ppm, and the peak intensity of choline at 3.21 ppm was increased. Conclusion: We found that decreasing lipids, an increasing lactate peak that split into two peaks, 1.26 ppm and 1.36 ppm, and an increasing choline peak at 3.22 ppm were markers of tumor invasion into the gastric tissue layers. This study implies that MR spectroscopy can be a useful diagnostic tool for gastric cancer.

• Journal of the Korean Ceramic Society
• /
• v.33 no.7
• /
• pp.743-754
• /
• 1996

In this study (Ba1-xPbx)TiO3 was synthesized by modified oxalate process in order to prevent vaporization of PbO through low temperature synthesis climinate Cl ion reproducibly substitute Pb for by and uniformly distribute ion (Ba1-xPbx)TiO3 was synthesized by coprecipitation of lead acetate barium acetate and ammonium titanyl oxalate have been used as starting materials. The substitution of Pb for Ba was reproducibly possible synthetic temperature of perovskite structure becomes lowed as the Pb concentration increases and fine partic-les (specific surface are :7.2 cm2/g) were obtained, BaTiO3 powders calcined at 90$0^{\circ}C$ for 3 hours were cubic from in XRD analysis and as Pb content was increases evident split of tetragonal peaks could be observed The optimum conditions to synthesize (Ba,Pb)TiO3 powder are the followings ; synthesis temperature (5$^{\circ}C$)

• Proceedings of the KOSOMBE Conference
• /
• v.1989 no.05
• /
• pp.19-22
• /
• 1989

This paper represents a syntactic peak detection algorithm which detects peaks in the ECG signal. In the algorithm, the input waveform is linearly approximated by "split-and-merge" method, and then each line segment is symbolized with primitive set. The peeks in the symbolized input waveform are recognized by the finite-state automata, which the deterministic finite-state language is parsed by. This proposed algorithm correctly detects peaks in a normal ECG signal as well as in the abnormal ECG signal such as tachycardia and the contaminated signal with noise.

• Korean Journal of Applied Biomechanics
• /
• v.21 no.1
• /
• pp.25-29
• /
• 2011

The purpose of this study was to analyze quantitative and qualitative differences according to shoe type for the grand jete landing in ballet. The subjects for this study were 9 female ballet majors with an average of 12 years of experience. Subjects jumped, performing a front split, and landed on 1 foot, a movement called the grand jete. Analysis was performed on the students' landing. Independent variables were 3 shoe types: split sole, traditional out sole, and 5-toed forefoot shoes, with bare feet as a control group. Dependent variables were vertical passive ground reaction force and qualitative elements. Passive ground reaction force variables(maximum passive peak value, number of passive peaks, passive force-time integral, and center of pressure) were measured by the Kistler 9281B Force Platform. Qualitative elements were comfort, cushioning, pain, and fit. Statistical analysis included both 1-way ANOVA and Tukey's test for follow-up. Finalized data demonstrated that the 5-toed forefoot shoe allows the forefoot to expand and the toes to individually press down upon landing, increasing foot contact with the surface. Five-toed forefoot shoes minimize passive peaks and pain, while increasing comfort, cushioning, and fit. Most ballet movements are composed of jumping, balancing, landing, and spinning. Wearing 5-toed forefoot shoes allows for a natural range of movement in each toe, to improve both technique and balance. Pain and injuries from ballet can be minimized by wearing the correct shoe type. According to this analysis, it is possible to customized ballet shoes to increase the efficiency of techniques and movements.

• Bulletin of the Korean Chemical Society
• /
• v.33 no.5
• /
• pp.1722-1728
• /
• 2012

During the analyzing processes of the compounds, some researchers are puzzled by the analytical signals for some TSNAs (with or without splitting peaks at various pHs and temperatures). In this work, a detailed theoretical study of structural and thermal properties of the $E/Z$ isomers of TSNAs and the corresponding protonated structures was performed using DFT methods. The calculations showed that the $E$ isomers are almost stable than $Z$ isomers, while the $Z$ isomers would be more stable when in protonation. The calculated results predicted the possibility of separation of their $E$ and $Z$ isomer forms and also showed that protonation affects the dipole moment of molecules significantly (0.1-0.5 to 0.7-2.1 Debye). The calculations agreed well with the experiments that the split-up of the HPLC signal for TSNAs into two peaks are very sensitive to the pH and temperature of the mobile-phase.