• Title/Summary/Keyword: spike

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In Vitro Wheat Immature Spike Culture Screening Identified Fusarium Head Blight Resistance in Wheat Spike Cultured Derived Variants and in the Progeny of Their Crosses with an Elite Cultivar

  • Huang, Chen;Gangola, Manu P.;Kutcher, H. Randy;Hucl, Pierre;Ganeshan, Seedhabadee;Chibbar, Ravindra N.
    • The Plant Pathology Journal
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    • v.36 no.6
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    • pp.558-569
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    • 2020
  • Fusarium head blight (FHB) is a devastating fungal disease of wheat (Triticum aestivum L.). The lack of genetic resources with stable FHB resistance combined with a reliable and rapid screening method to evaluate FHB resistance is a major limitation to the development of FHB resistant wheat germplasm. The present study utilized an immature wheat spike culture method to screen wheat spike culture derived variants (SCDV) for FHB resistance. Mycotoxin concentrations determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS) correlated significantly (P < 0.01) with FHB severity and disease progression during in vitro spike culture. Selected SCDV lines assessed for FHB resistance in a Fusarium field disease nursery in Carman, Manitoba, Canada in 2016 showed significant (P < 0.01) correlation of disease severity to the in vitro spike culture screening method. Selected resistant SCDV lines were also crossed with an elite cv. CDC Hughes and the progeny of F2 and BC1F2 were screened by high resolution melt curve (HRM) analyses for the wheat UDP-glucosyl transferase gene (TaUGT-3B) single nucleotide polymorphism to identify resistant (T-allele) and susceptible (G-allele) markers. The progeny from the crosses were also screened for FHB severity using the immature spike culture method and identified resistant progeny grouped according to the HRM genotyping data. The results demonstrate a reliable approach using the immature spike culture to screen for FHB resistance in progeny of crosses in early stage of breeding programs.

Flicker Prevention and Noise Reduction Using Edge-Spike Modulation in Visible Light Communication

  • Lee, Seong-Ho
    • Journal of Sensor Science and Technology
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    • v.27 no.3
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    • pp.143-149
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    • 2018
  • In this paper, we introduce an edge-spike modulation method for visible light communication (VLC). This method is effective in preventing LED flicker and 120 Hz noise interference in base-band VLC. In the VLC transmitter, edge-spikes are generated by passing the digital data through a simple RC-high pass filter (HPF). The LED modulation of the edge-spikes does not change the average power of the LED light; thus it prevents LED flicker. In the VLC receiver, the 120 Hz noise from other lighting lamps is easily eliminated by RC-HPF, while the edge-spike signal is detected normally. In our experiment, the message of an air-quality sensor was successfully transmitted using edge-spike modulation. This structure is useful in constructing, e.g., wireless gas monitoring sensor systems to warn and prevent harmful gas leakage accidents in buildings using LED light.

Stall Inception Characteristics of Axial Compressor Varying IGV Stagger (축류압축기의 입구안내깃 각도에 따른 스톨선구신호 특성 연구)

  • Bae, Hyo-Jo;Lim, Hyung-Soo;Song, Seung-Jin;Kang, Shin-Hyoung;Yang, Soo-Seok
    • The KSFM Journal of Fluid Machinery
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    • v.15 no.1
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    • pp.52-57
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    • 2012
  • Stall inception characteristics are researched to understand stall well. To realize different stall inception patterns, IGV stagger angle was changed. At design IGV stagger angle, spike, which is short length scale, is observed. Decreasing IGV stagger angle, spike changes to mode, which is long length scale, and further decreasing get multi cell. Compressor maps for each IGV stagger are shown to compare different stall inceptions. The characteristics of both spike and mode are confirmed in this experiment. Furthermore, transient from spike to mode is find. multi cell has 4cells and is little bit faster than mode. and multi cell shows 2nd, 3rd characteristics on compressor map.

Root Cause and Countermeasure on the Spike Vibration of a 550MW Class USC(Ultra Super Critical) Steam Turbine (550MW급 초초임계압(USC, Ultra Super Critical) 증기터빈의 Spike Vibration 에 관한 원인 규명 및 대책)

  • Yang, Seong-Heon;Kim, Yong-Seok;Nah, Un-Hak
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2007.05a
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    • pp.442-447
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    • 2007
  • A very abnormal vibration was occurred at the LP(low pressure) turbine continuously during the pre-operation for a 550MW class USC(ultra super critical) steam turbine. This vibration was initiated at the rotating speed of about 3,450rpm and then the vibration amplitude was highly increased the number by $2{\sim}3$ times with the increase of the rotating speed to the rated speed(3,600rpm). In this paper, this abnormal vibration named spike vibration. This spike vibration was caused by the rubbing between the rotating bucket tip seal and the Lower Half of spill strip. Also, this paper presents the mechanism of the spike vibration and the proper method to eliminate this abnormal vibration problem. This result would be good practice to find the solution of similar high vibration in the USC steam turbines for power plant as well as industrial rotating machineries.

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Micro-tissue collecting tool for diagnosis of micro-spike biopsy (마이크로 스파이크로 채취한 조직의 진단을 위한 미세 조직회수도구)

  • Jeong, Hyo-Young;Koo, Kyo-In;Lee, Sang-Min;Ban, Jae-Won;Park, Ho-Soo;Bang, Seoung-Min;Song, Si-Young;Cho, Dong-Il Dan
    • Journal of Sensor Science and Technology
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    • v.18 no.2
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    • pp.122-127
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    • 2009
  • We have developed and reported several micro-spikes for minimally invasive biopsy. In this paper, a micro-tissue collecting tool for tissue diagnosis extracted by micro-spike is presented. Using proposed polydimethy-siloxane (PDMS) micro-tissue collecting tool, which has a negative micro-spike structure in a porous chamber, the extracted tissue in a micro-spike is effectively detached. The gastro-intestinal tissue of a pig is extracted in an in vivo environment, and then it is detached from a micro-spike using the PDMS micro-tissue collecting tool. A fine clinical picture of the detached tissue is acquired.

Root Cause and Countermeasure on the Spike Vibration of a 550MW Class USC(ultra super critical) Steam Turbine (550MW급 초초임계압(USC, ultra super critical) 증기터빈의 Spike Vibration에 관한 원인 규명 및 대책)

  • Yang, Seong-Heon;Kim, Yong-Seok;Nah, Un-Hak;Park, Jong-Geun
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.17 no.12
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    • pp.1238-1245
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    • 2007
  • A very abnormal vibration was occurred at the LP(low pressure) turbine continuously during the pre-operation for a 550MW class USC(ultra super critical) steam turbine. This vibration was initiated at the rotating speed of about 3,450 rpm and then the vibration amplitude was highly increased the number by $2{\sim}3$ times with the increase of the rotating speed to the rated speed (3,600 rpm). In this paper, this abnormal vibration named spike vibration. This spike vibration was caused by the rubbing between the rotating bucket tip seal and the lower half of spill strip. Also, this paper presents the mechanism of the spike vibration and the proper method to eliminate this abnormal vibration problem. This result would be good practice to find the solution of similar high vibration in the USC steam turbines for power plant as well as industrial rotating machineries.

Determination of Li by Isotope Dilution Inductively Coupled Plasma Mass Spectrometry

  • Park, Chang J.;Chung, Bag S.
    • Analytical Science and Technology
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    • v.8 no.4
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    • pp.427-434
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    • 1995
  • Inductively coupled plasma mass spectrometry combined with the isotope dilution method is used for the determination of lithium. The isotope dilution method is based on the addition of a known amount of enriched isotope (spike) to a sample. The analyte concentration is obtained by measuring the altered isotope ratio. The spike solution is calibrated through so called reverse isotope dilution with a primary standard. The spike calibration is an important step to minimize error in the determined concentration. It has been found essential to add spike to a sample and the primary standard so that the two isotope ratios should be as dose as possible. Since lithium is neither corrosive nor toxic, lithium is used as a chemical tracer in the nuclear power plants to measure feedwater flow rate. 99.9% $^7Li$ was injected into a feedwater line of an experimental system and sample were taken downstream to be spiked with 95% $^6Li$ for the isotope dilution measurements. Effects of uncertainties in the spike enrichment and isotope ratio measurement error at various spike-to-sample ratios are presented together with the flow rate measurement results in comparison with a vortex flow meter.

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Neuronal Spike Train Decoding Methods for the Brain-Machine Interface Using Nonlinear Mapping (비선형매핑 기반 뇌-기계 인터페이스를 위한 신경신호 spike train 디코딩 방법)

  • Kim, Kyunn-Hwan;Kim, Sung-Shin;Kim, Sung-June
    • The Transactions of the Korean Institute of Electrical Engineers D
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    • v.54 no.7
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    • pp.468-474
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    • 2005
  • Brain-machine interface (BMI) based on neuronal spike trains is regarded as one of the most promising means to restore basic body functions of severely paralyzed patients. The spike train decoding algorithm, which extracts underlying information of neuronal signals, is essential for the BMI. Previous studies report that a linear filter is effective for this purpose and there is no noteworthy gain from the use of nonlinear mapping algorithms, in spite of the fact that neuronal encoding process is obviously nonlinear. We designed several decoding algorithms based on the linear filter, and two nonlinear mapping algorithms using multilayer perceptron (MLP) and support vector machine regression (SVR), and show that the nonlinear algorithms are superior in general. The MLP often showed unsatisfactory performance especially when it is carelessly trained. The nonlinear SVR showed the highest performance. This may be due to the superiority of the SVR in training and generalization. The advantage of using nonlinear algorithms were more profound for the cases when there are false-positive/negative errors in spike trains.

Genetic Analyses on Quantitative Characters of F1 Generation in Malting Barley (맥주맥 F1세대의 양적형질에 대한 유전분석)

  • 정원복;오주성;황필성
    • Journal of Life Science
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    • v.13 no.1
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    • pp.29-33
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    • 2003
  • The study was conducted to get genetic information on quantitative characters of $F_1$ in barley through diallel crosses using seven malting barley. The results obtained were summarized as follows. Additive, dominant, maternal, and reciprocal effort were observed in culm length, spike length, awn length, culm diameter, grain length, grain width, ]lumber of grains per spike, and 1000 grain weight. Based on the Vr-Wr graphical analysis, culm length, spike length, awn length, grain length, and number of grains per spike were found to be inherited over dominance. Dominant effects were higher than additive effects in culm length, spike length, awn length, culm diameter, grain length, grain width, number of grains per spike, and 1000 grain weight. The narrow- sense heritability showed high value as 40.06% for spike length.

Effects of Boron Applications on Flower Spike Dieback of Statice (Limonium spp.)

  • Choi, Chang-Hak;Jeong, Dong-Chun;Lee, Jin-Jae;Song, Young-Ju;Ahn, Byung-Koo;Lee, Jin-Ho
    • Korean Journal of Soil Science and Fertilizer
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    • v.46 no.3
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    • pp.209-215
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    • 2013
  • This study was conducted to investigate the effects of boron treatments on flower spike dieback of Statice (Limonium spp.) grown in soilless hydroponic and soil cultures under rain shelter system. The growth of Statice was gradually improved with increasing boron applications in the hydroponics, but not in soil culture with boron treatment as foliar spray or soil application. The degree of flower spike dieback in 6 levels (0, no dieback incidence to 5, very severe dieback incidence) ranged between 0.5 with boron application and 4.4 with no boron treatment. The content of boron in Statice flower spike increased with increasing rates of boron applications in the hydroponics, but the contents of P, Mg, and N were not affected by the boron application. However, K content was highest with no boron treatment. In soil culture, incidence of flower spike dieback decreased with foliar spray or soil application of boron. Therefore, boron application was effective in reducing flower spike dieback and improving cut-flower productivity and its quality, and the recommended rates of boron application were $50{\sim}80{\mu}gL^{-1}$ for hydroponics culture whereas 0.2% borex or ${\geq}0.4kg\;10a^{-1}$ boric acid by foliar spray application for soil culture.