• Asian Pacific Journal of Cancer Prevention
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• v.16 no.14
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• pp.6073-6080
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• 2015

Background: Melanoma differentiation-associated gene-7 (MDA-7)/interleukin-24 (IL-24), a unique tumor suppressor gene, has killing activity in a broad spectrum of cancer cells. Herein, plasmids producing mda-7 proteins fused to different RGD peptides (full RGD4C and shortened RGD, tRGD) were evaluated for apoptosis induction with a hepatocellular carcinoma cell line, Hep-G2. The study aim was to improve the apoptosis potency of mda-7 by tethering to RGD peptides. Materials and Methods: Three plasmids including mda-7, mda-7-RGD and mda-7-tRGD genes beside a control vector were transfected into Hep-G2 cells. After 72 hours incubation, cell viability was evaluated by MTT assay. In addition, the rate of apoptosis was analyzed by flow cytometry using PI/annexin staining. To detect early events in apoptosis, 18 hours after transfection, expression of the BAX gene was quantified by real time PCR. Modeling of proteins was also performed to extrapolate possible consequences of RGD modification on their structures and subsequent attachment to receptors. Results and Conclusions: In MTT assays, while all mda-7 forms showed measurable inhibition of proliferation, unmodified mda-7 protein exhibited most significant effect compared to control plasmid (P<0.001). Again, flow cytometry analysis showed a significant apoptosis induction by simple mda-7 gene but not for those RGD-fused mda-7 proteins. These findings were also supported by expression analysis of BAX gene (P<0.001). Protein modelling analysis revealed that tethering RGD at the end of IL-24/Mda7 disrupt attachment to cognate receptor, IL-20R1/IL-20R2. In conclusion, fusion of RGD4C and shortened RGD peptides to carboxyl terminal of mda7, not only reduce apoptosis property in vitro but also disrupt receptor attachment as demonstrated by protein modelling.

• Journal of Korea Multimedia Society
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• v.13 no.5
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• pp.710-721
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• 2010

Recently, for the advancement of embedded technology about mobile device, a new kind of service, mash-up is appeared. It is service or application combining multimedia content making tool or device and web-GIS(geographic information system) service in the mobile environment. This service can be ease to use for casual user and can apply in various ways. So, It is served in web 2.0 environment actively. But, in the mashup service, because generated multimedia contents linked with web map are new type of multimedia contents which include user's migration routes in the space such as GPS coordinates. Thus, there are no protection ways for intellectual property created by GIS web-map service users and user's privacy. In this paper, we proposed a location and user information hiding scheme for GIS web-map service. This scheme embeds location and user information into a picture that is taken by camera module on the mobile phone. It is not only protecting way for user's privacy but is also tracing way against illegal photographer who is peeping person through hidden camera. And than, we also realized proposed scheme on the mobile smart phone. For minimizing margin of error about location coordinate value against contents manipulating attacks, GPS information is embedded into chrominance signal of contents considering weight of each digit about binary type of GPS coordinate value. And for tracing illegal photographer, user information such as serial number of mobile phone, phone number and photographing date is embedded into frequency spectrum of contents luminance signal. In the experimental results, we confirmed that the error of extracted information against various image processing attacks is within reliable tolerance. And after file format translation attack, we extracted embedded information from the attacked contents without no damage. Using similarity between extracted one and original templete, we also extracted whole information from damaged chrominance signal of contents by various image processing attacks.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2017.05a
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• pp.77-77
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• 2017

Titanium and its alloys offer attractive properties in a variety of applications. These are widely used for the field of biomedical implants because of its good biocompatibility and high corrosion resistance. Titanium anodizing is often used in the metal finishing of products, especially those can be used in the medical devices with dense oxide surface. Based on SAE/AMS (Society of Automotive Engineers/Aerospace Material Specification) 2488D, it has the specification for industrial titanium anodizing that have three different types of titanium anodization as following: Type I is used as a coating for elevated temperature forming; Type II is used as an anti-galling coating without additional lubrication or as a pre-treatment for improving adherence of film lubricants; Type III is used as a treatment to produce a spectrum of surface colours on titanium. In this study, we have focused on Type II anodization for the medical (dental and orthopedic) application, the anodized surface was modified with gray color under alkaline electrolyte. The surface characteristics were analyzed with Focused Ion Beam (FIB), Scanning Electron Microscopy (SEM), surface roughness, Vickers hardness, three point bending test, biocompatibility, and corrosion (potentiodynamic) test. The Ti-6Al-4V alloy was used for specimen, the anodizing procedure was conducted in alkaline solution (NaOH based, pH>13). Applied voltage was range between 20 V to 40 V until the ampere to be zero. As results, the surface characteristics of anodic oxide layer were analyzed with SEM, the dissecting layer was fabricated with FIB method prior to analyze surface. The surface roughness was measured by arithmetic mean deviation of the roughness profile (Ra). The Vickers hardness was obtained with Vickers hardness tester, indentation was repeated for 5 times on each sample, and the three point bending property was verified by yield load values. In order to determine the corrosion resistance for the corrosion rate, the potentiodynamic test was performed for each specimen. The biological safety assessment was analyzed by cytotoxic and pyrogen test. Through FIB feature of anodic surfaces, the thickness of oxide layer was 1.1 um. The surface roughness, Vickers hardness, bending yield, and corrosion resistance of the anodized specimen were shown higher value than those of non-treated specimen. Also we could verify that there was no significant issues from cytotoxicity and pyrogen test.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.36 no.1
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• pp.33-44
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• 2000

Ships in a seaway will encounter dangerous situation, such as slamming, stranding, and capsizing. The number of capsizing is small, but the loss due to them is very large from the viewpoint of human life, property, and the environmental pollution. The number of capsizing of fishing vessels is about 62% of total number of capsizing, and the half of them is originated from the operational mistake in a seaway. So the dynamics and the capsizing phenomena are to be studied, and the guide for the safe operation of a fishing vessel in a seaway are to be specified. The hydrodynamic forces consist of radiation forces (which are due to the motion of a ship), Froude-Krylov forces (which is due to the incoming waves), and diffraction forces (which is due to the wave and ship interaction). These forces are calculated by well-known strip method. Using the calculated forces, the motion of a ship in a regular sea is obtained. In the real seaway, the waves are very irregular, therefore the statistical analysis is very helpful. In this paper, using the results of the motion in a regular seaway and the wave spectrum, the motion in a irregular seaway are obtained and analyzed.

• Journal of Conservation Science
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• v.33 no.5
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• pp.391-398
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• 2017

This study was conducted to investigate lichen as a typical biomass damage on the surfaces of flagpole-supporting stones in the Beopjusa temple. The lichens present on the flagpole-supporting stones were limited to five species. Two dominant lichen species were identified: Aspicilia sp. and Pertusaria flavicans. One foliose species and one fruticose species, which are rarely observed on crustose lichens, were identified as Xanthoparmelia conspersa and Ramalina sekika, respectively. The lichen inhabiting the black algae layer was confirmed as Leprocaulon textum. ATR-FTIR was performed to analyze the secondary metabolites synthesized by the lichens. By comparing the FTIR spectra of Xanthoparmelia conspersa and Ramalina sekika, the synthesized organic acids were confirmed to differ from each other. Furthermore, the spectral changes and characteristics due to functional groups in the molecules were confirmed.

• Polymer(Korea)
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• v.37 no.1
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• pp.5-14
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• 2013

In this paper the effect of UV (ultraviolet) exposure on HDPE (high density polyethylene)-smooth and f-PP (flexible polypropylene) geomembranes is evaluated under UVB-313 (ultraviolet wavelength 290-315 nm) exposure. Tensile property, melt flow index (MFI), oxidation induction time (OIT), both standard-OIT and high pressure-OIT and Fourier transform infrared spectroscopy/attenuated total reflectance (FTIR/ATR) results are discussed. Although tensile properties of the exposed geomembrane samples remained unchanged, the depletion of antioxidants was found higher for f-PP than for HDPE geomembrane. Arrhenius model by extrapolation was used on the data to predict the antioxidant lifetime to a typical site temperature of $20^{\circ}C$. There was no significant difference between the MFI value of the virgin and UV exposed HDPE geomembrane samples but a decrease in MFI was found in f-PP geomembrane that signifies that crosslinking has occurred. From FTIR spectra, the small peak (near $1750\;cm^{-1}$) observed in the spectrum of UV exposed sample corresponds to a carbonyl (C=O) linkage, which suggests that oxidation has occurred in the polymer structure, and another new band for f-PP between 3100 and $3500\;cm^{-1}$ is attributed to a hydroxyl bond and/or hydroperoxide bond.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.17 no.5
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• pp.1213-1218
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• 2013

In this study, Ga-doped ZnO (GZO) thin films were fabricated on transparent sapphire substrate by RF magnetron sputtering method and then investigated the effect of various substrate temperature on the electrical, optical properties and characteristic of crystallization of the GZO thin films. The electrical property indicated that the lowest resistivity ($4.18{\times}10^{-4}{\Omega}cm$), the highest carrier concentration ($6.77{\times}10^{20}cm^{-3}$) and Hall mobility ($22cm^2/Vs$) were obtained in the GZO thin film fabricated at $300^{\circ}C$. And for this condition, the highest c-axis orientation and (002) diffraction peak which exhibits a FWHM of $0.34^{\circ}$ were obtained. From the results of AFM measurements, it is known that the highest crystallinity is observed at $300^{\circ}C$. The transmittance spectrum in the visible range was approximately 80 % regardless of substrate temperature. The optical band-gap showed the blue-shift as increasing the substrate temperature to $300^{\circ}C$, and they are all larger than the band gap of bulk ZnO (3.3 eV). It can be explained by the Burstein-Moss effect.

• Applied Chemistry for Engineering
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• v.22 no.2
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• pp.138-143
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• 2011

To investigate the electromagnetic interference shielding efficiency (EMI SE) property based on heat treatment effects of carbon fibers in various temperatures, the polyacrilonitrle-based carbon fibers were prepared by electrospinning method and treated at 1073, 1323, 1873 and 2573 K. The surface morphology of carbon fibers was investigated by using FE-SEM and the carbon crystallization was studied by Raman spectroscopy based on effects of reaction temperatures. The electrical conductivity was obtained by measuring the surface resistance with four probe method on carbon crystallization. The permittivity, permeability and EMI SE were investigated by using S-parameter in the range of 800~4500 MHz. In case of carbon fibers treated at 2573 K, the improved carbon crystallization was confirmed by Raman spectrum and the enhanced electrical conductivity showing 54.7 S/cm was also observed. The permittivity was dramatically improved by factor of 4 based on effect of high reaction temperature. Eventually, the highly improved EMI SE value was obtained showing around 41.7 dB.

• Journal of the Earthquake Engineering Society of Korea
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• v.26 no.5
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• pp.191-202
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• 2022

Markov envelope as a theoretical solution of the parabolic wave equation with Markov approximation for the von Kármán type random medium is studied and approximated with the convolution of two probability density functions (pdf) of normal and gamma distributions considering the previous studies on the applications of Radiative Transfer Theory (RTT) and the analysis results of earthquake records. Through the approximation with gamma pdf, the constant shape parameter of 2 was determined regardless of the source distance r_o. This finding means that the scattering process has the property of an inhomogeneous single-scattering Poisson process, unlike the previous studies, which resulted in a homogeneous multiple-scattering Poisson process. Approximated Markov envelope can be treated as the normalized mean square (MS) envelope for ground acceleration because of the flat source Fourier spectrum. Based on such characteristics, the path duration is estimated from the approximated MS envelope and compared to the empirical formula derived by Boore and Thompson. The results clearly show that the path duration increases proportionately to r_o^1/2-r_o², and the peak value of the RMS envelope is attenuated by exp (-0.0033r_o), excluding the geometrical attenuation. The attenuation slope for r_o≤100 km is quite similar to that of effective attenuation for shallow crustal earthquakes, and it may be difficult to distinguish the contribution of intrinsic attenuation from effective attenuation. Slowly varying dispersive delay, also called the medium effect, represented by regular pdf, governs the path duration for the source distance shorter than 100 km. Moreover, the diffraction term, also called the distance effect because of scattering, fully controls the path duration beyond the source distance of 300 km and has a steep gradient compared to the medium effect. Source distance 100-300 km is a transition range of the path duration governing effect from random medium to distance. This means that the scattering may not be the prime cause of peak attenuation and envelope broadening for the source distance of less than 200 km. Furthermore, it is also shown that normal distribution is appropriate for the probability distribution of phase difference, as asserted in the previous studies.

• Geomechanics and Engineering
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• v.32 no.3
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• pp.307-319
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• 2023

Ground fissures have a huge effect on the integrity of surface structures. In high-intensity ground fissure regions, however, land resource would be wasted and city building and economic development would be limited if the area avoiding principle was used. In view of this challenge, to reveal the seismic response and seismic failure characteristics of ground fissure sites, a shaking table test on model soil based on a 1:15 scale experiment was carried out. In the test, the spatial distribution characteristics of acceleration response and Arias intensity were obtained for a site exposed to earthquakes with different characteristics. Furthermore, the failure characteristics and damage evolution of the model soil were analyzed. The test results indicated that, with the increase in the earthquake acceleration magnitude, the crack width of the ground fissure enlarged from 0 to 5 mm. The soil of the hanging wall was characterized by earlier cracking and a higher abundance of secondary fissures at 45°. Under strong earthquakes, the model soil, especially the soil near the ground fissure, was severely damaged and exhibited reduced stiffness. As a result, its natural frequency also decreased from 11.41 Hz to 8.05 Hz, whereas the damping ratio increased from 4.8% to 9.1%. Due to the existence of ground fissure, the acceleration was amplified to nearly 0.476 m/s², as high as 2.38 times of the input acceleration magnitude. The maximum of acceleration and Arias intensity appeared at the fissure zone, which decreased from the main fissure toward both sides, showing hanging wall effects. The seismic intensity, duration and frequency spectrum all had certain effects on the seismic response of the ground fissure site, but their influence degrees were different. The seismic response of the site induced by the seismic wave that had richer low-frequency components and longer duration was larger. The discrepancies of seismic response between the hanging wall and the footwall declined obviously when the magnitude of the earthquake acceleration increased. The research results will be propitious to enhancing the utilizing ratio of the limited landing resource, alleviation of property damages and casualties, and provide a good engineering application foreground.