• Title/Summary/Keyword: spectroscopic techniques

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Selective Cytotoxicity of Novel Platinum(II) Coordination Complexes Containing DL-2-Hydroxy 3-Methylbutyric Acid (DL-2 하이드록시 3-메틸 부틸산물 배위자로 한 새로운 항암성 백금(II) 착체의 위암세포와 정상신장세포에 대한 선택적 세포독성)

  • 정지창;홍언표;최승기;장성구;육창수;노영수
    • Biomolecules & Therapeutics
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    • v.11 no.2
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    • pp.91-98
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    • 2003
  • A new series of highly water soluble platinum(II) complexes[Pt(II)(DL-2-hydroxy-3-methylbutyrate)(trans-l-1,2-dimninocyc1ohexane)] (PC-1) and [Pt(II)DL-2-hydroxy-3-methylbutyrate](cis-1,2-diaminocyclohexane)](PC-2) were synthesized and characterized by their elemental analysis and by various spectroscopic techniques [infrared(IR), $^{13}C$-nuclear magnetic resonance (NMR)]. In vitro antitumor activity of new Pt(II)complexes was tested against MKN-45, MKN/ADM and MKN/CDDP human gastric adenocarcinoma cell lines using colorimetric MTT[3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyltetrazoliumbromide] assay for cell survival and proliferation. PC-1 and PC-2 showed active against MKN-45/P, MKN/ADM and MKN/CDDP human gastric cancer cell lines, and the antitumor activity of these compounds were comparable or superior to that of cisplatin. The nephrotoxicities of PC-1 and PC-2 were found quite less then that of cisplatin using MTT and [$^3H$] thymidine uptake tests in rabbit proximal tubule cells, human kidney cortical cells human renal cortical tissues. Based on these results, these novel platinum(II) complex compounds(PC-1 & PC-2) represent a valuable lead in the development of the new anticancer chemotherapeutic agents capable of improving antitumor activity and low nephrotoxicity.

Effect of Thiourea on the Copper Electrodeposition (구리 전기 도금에 Thiourea가 미치는 효과)

  • Lee, Joo-Yul;Yim, Seong-Bong;Hwang, Yang-Jin;Lee, Kyu-Hwan
    • Journal of Surface Science and Engineering
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    • v.43 no.6
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    • pp.289-296
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    • 2010
  • The effect of organic additives, thiourea (TU), on the copper electroplated layer of large rectangular size was investigated through physical and various electrochemical techniques. It was found that TU had strong adsorption characteristics on the Ni substrate and affected the initial electroplating process by inducing surface reaction instead of mass transfer in the bulk solution. TU additives had its critical micelle concentration at 200 ppm in copper sulphate solution and showed abrupt change in morphological and electrochemical impedance spectroscopic results around this concentration, which could be related with the destruction of adsorption structure of TU-Cu(I) complex formed at the Ni substrate surface. By conducting a commercial electroplating simulation, when TU additives was included at cmc in the plating solution, it acted as a depolarizer for copper electrodeposition and was effective to reduce the unevenness of copper deposits between centre and edge region at high current densities of 10 ASD.

Design and Engineering of Antimicrobial Peptides Based on LPcin-YK3, an Antimicrobial Peptide Derivative from Bovine Milk

  • Kim, Ji-Sun;Jeong, Ji-Ho;Kim, Yongae
    • Journal of Microbiology and Biotechnology
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    • v.28 no.3
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    • pp.381-390
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    • 2018
  • We have previously derived a novel antimicrobial peptide, LPcin-YK3(YK3), based on lactophoricin and have successfully studied and reported on the relationship between its structure and function. In this study, antimicrobial peptides with improved antimicrobial activity, less cytotoxicity, and shorter length were devised and characterized on the basis of YK3, and named YK5, YK8, and YK11. The peptide design was based on a variety of knowledge, and a total of nine analog peptides consisted of one to three amino acid substitutions and C-terminal deletions. In detail, tryptophan substitution improved the membrane perturbation, lysine substitution increased the net charge, and excessive amphipathicity decreased. The analog peptides were examined for structural characteristics through spectroscopic analytical techniques, and antimicrobial susceptibility tests were used to confirm their activity and safety. We expect that these studies will provide a platform for systematic engineering of new antibiotic peptides and generate libraries of various antibiotic peptides.

Study on UV Absorption Materials Derived from Red Algae Gloiopeltis fucatas and Mazzaella sp. in Russia

  • Lee, Moon-Hee;Kim, Yeon-Kye;Yoon, Na Young;Shim, Kil Bo;Aminina, Natalya M.;Kadnikova, Irina A.;Lim, Chi-Won
    • Fisheries and Aquatic Sciences
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    • v.15 no.4
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    • pp.361-363
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    • 2012
  • We investigated ultraviolet (UV) absorption materials from Russian seaweeds. First, the UV absorptivities of five seaweeds Gloiopeltis fucatas, Mazzaella sp., Mastocarpus pacificus, Laminaria cichorioides, Saccharina japonica were evaluated by a UV spectrometer. Of these seaweeds, Gloiopeltis fucatas and Mazzaella sp. showed high levels of UV absorption. Column chromatography of active 50% aqueous ethanol extracts from Gloiopeltis fucatas and Mazzaella sp. resulted in the purification of two known compounds. Spectroscopic techniques identified their structures as shinorine and palythine. These materials exhibited UV absorptive capabilities at wavelengths of 333 and 320 nm, respectively. These results suggest that Gloiopeltis fucatas and Mazzaella sp. may be useful as natural cosmeceutical sources.

Acetylcholinesterase Inhibitors from the Aerial Parts of Corydalis speciosa

  • Kim, Dae-Keun;Lee, Ki-Taek;Kim, Sung-Hoon;Park, Hee-Wook;Lim, Jong-Pil;Shin, Tae-Yong;Eom, Dong-Ok;Yang, Jae-Heon;Eun, Jae-Soon;Baek, Nam-In
    • Archives of Pharmacal Research
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    • v.27 no.11
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    • pp.1127-1131
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    • 2004
  • In a bioassay-guided search for acetylcholinesterase inhibitors from Korean natural resources, four isoquinoline alkaloids, corynoxidine (1), protopine (2), palmatine (3), and berberine (4) have been isolated from the methanolic extract of the aerial parts of Corydalis speciosa. Structures of these compounds were elucidated on the basis of spectroscopic techniques. These compounds inhibited acetylcholinesterase activity in a dose-dependent manner, and the $IC_50$ values of compounds 1-4 were 89.0, 16.1, 5.8, and 3.3 $\mu$ M, respectively.

The Allosteric Transition of the Chaperonin GroEL from Escherichia coli as Studied by Solution X-Ray Scattering

  • Kuwajima Kunihiro;Inobe Tomonao;Arai Munehito
    • Macromolecular Research
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    • v.14 no.2
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    • pp.166-172
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    • 2006
  • This is a short review article of our recent studies on the ATP-induced, allosteric conformational transition of the chaperonin GroEL complex by solution X-ray scattering. We used synchrotron X-ray scattering with a two-dimensional, charge-coupled, device-based X-ray detector to study (1) the specificity of the chaperonin GroEL for its ligand that induced the allosteric transition, and (2) the identification of the allosteric transition of GroEL in its complicated kinetics induced by ATP. Due to the dramatically increased sensitivity of the X-ray scattering technique based on the use of the two dimensional X-ray detector and synchrotron radiation, different allosteric conformational states of GroEL populated under different conditions were clearly distinguished from each other. It was concluded that solution X-ray scattering is an extremely powerful tool for investigating the equilibrium and kinetics of cooperative conformational transitions of oligomeric protein complex, especially when combined with other spectroscopic techniques such as fluorescence spectroscopy.

Two New Phenolic Compounds from the Fruiting Bodies of Ganoderma tropicum

  • Hu, Li-Li;Ma, Qing-Yun;Huang, Sheng-Zhuo;Guo, Zhi-Kai;Guo, Jian-Chun;Dai, Hao-Fu;Zhao, You-Xing
    • Bulletin of the Korean Chemical Society
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    • v.34 no.3
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    • pp.884-886
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    • 2013
  • Chemical investigation of the fruiting bodies of Ganoderma tropicum led to the isolation of two new phenolic compounds, ganodermatropins A (1) and B (2). Their structures were elucidated by spectroscopic techniques (MS, 1D and 2D NMR). Ganodermatropin A exhibited antimicrobial activity against Staphylococcus aureus.

Phenolic Components from the Fruits of Pourthiaea villosa (윤노리나무 과실의 페놀성 성분)

  • Lee, Hyun Jin;Ahn, Dalrae;Lee, Eun Byeol;Lee, Tae Gwan;Kim, Dae Keun
    • Korean Journal of Pharmacognosy
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    • v.44 no.1
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    • pp.16-21
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    • 2013
  • The fruits of Pourthiaea villosa were extracted with methanol and its extract was fractionated with n-hexane, methylene chloride, ethyl acetate and n-butanol. Repeated column chromatography of silica gel, sephadex LH-20 and HPLC led to the isolation of nine phenolic compounds from ethyl acetate soluble fraction. The chemical structures were elucidated as kaemferol-3-O-${\beta}$-D-glucopyranoside (astragalin) (1), isorhamnetin-3-O-${\beta}$-D-glucopyranoside (2), kaempferol-3-O-${\beta}$-D-xylopyranosyl($1{\rightarrow}2$)-${\alpha}$-L-rhamnopyranoside (3), caffeic acid (4), quercetin-3-O-${\beta}$-D-xylopyranosyl($1{\rightarrow}2$)-a-L-rhamnopyranoside (5), quercetin-3-O-${\beta}$-D-xylopyranosyl($1{\rightarrow}2$)-${\beta}$-D-glucopyranoside (6), quercetin-3-O-${\beta}$-D-xylopyranosyl($1{\rightarrow}2$)-${\beta}$-D-galactopyranoside (7), quercetin-3-O-${\alpha}$-L-rhamnopyranoside (quercitrin) (8), and kaempferol-3-O-${\alpha}$-L-rhamnopyranoside (afzelin) (9) by spectroscopic techniques. These compounds were isolated from this plant for the first time.

Selective catalytic reduction of NO by hydrocarbons over $Cu/Al_2O_3$ catalysts

  • Nam, Chang-Mo;Bernard M. Gibbs
    • Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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    • v.4 no.4
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    • pp.201-208
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    • 2000
  • The reduction of NO by hydrocarbons was investigated over Cu/Al$_2$O$_3$catalysts using a stainless steel flow reactor under highly oxidising diesel exhaust conditions(up to 15%). Three different Cu loadings(1,5 and 10wt.%) on an $Al_2$O$_3$support were prepared and characterized using spectroscopic techniques. The catalytic activity tests show that different Cu loadings as well as temperature, oxygen, and hydrocarbon concentration levels significantly influence the NO reduction. Increasing Cu loadings up to 5 and 10wt.% decreases the catalytic activities for NO reduction due to the formation of a bulk crystalline CuO phase, as observed from XRD and SEM images. In particular, the visualization of the copper dispersion on the surface using the SEM-BEI technique provides information on the extent of copper saturation, particle size, and the effects on NO reduction. However, the lower Cu loading(1 wt.%) increases the catalytic activity with a temperature window of 720-810K, thereby favoring the formation of well dispersed isolated Cu species, e.g. Cu(sup)2+ ions, which is related to selective NO reduction. The effects of other reaction parameters, such as oxygen, the hydrocarbon level and type, and byproduct emissions are further discussed.

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Purification and Spectroscopic Characterization of the Human Protein Tyrosine Kinase-6 SH3 Domain

  • Koo, Bon-Kyung;Kim, Min-Hyung;Lee, Seung-Taek;Lee, Weon-Tae
    • BMB Reports
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    • v.35 no.3
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    • pp.343-347
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    • 2002
  • The human protein tyrosine kinase-6 (PTK6) polypeptide that is deduced from the cDNA sequence contains a Src homology (SH) 3 domain, SH2 domain, and catalytic domain of tyrosine kinase. We initiated biochemical and NMR characterization of PTK6 SH3 domain in order to correlate the structural role of the PTK6 using circular dichroism and heteronuclear NMR techniques. The circular dichroism data suggested that the secondary structural elements of the SH3 domain are mainly composed of $\beta$-sheet conformations. It is most stable when the pH is neutral based on the pH titration data. In addition, a number of cross peaks at the low-field area of the proton chemical shift of the NMR spectra indicated that the PTK6 SH3 domain retains a unique and folded conformation at the neutral pH condition. For other pH conditions, the SH3 domain became unstable and aggregated during NMR measurements, indicating that the structural stability is very sensitive to pH environments. Both the NMR and circular dichroism data indicate that the PTK6 SH3 domain experiences a conformational instability, even in an aqueous solution.