• Research in Plant Disease
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• v.15 no.3
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• pp.222-229
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• 2009

A Pseudomonas strain SG3 producing biosurfactant and showing antifungal and insecticidal activities was isolated from agricultural soil severely contaminated with machine oils. The antagonistic bacterium inhibited mycelial growth of all of the tested fungal pathogens. The fermentation broth of SG3 also effectively suppressed the development of various plant diseases including rice blast, tomato gray mold, tomato late blight, wheat leaf rust, barley powdery mildew and red pepper anthracnose. An antifungal substance was isolated from the fermentation broth of SG3 by ethyl acetate partitioning, silica gel column chromatography and preparative HPLC under the guide of bioassay. The chemical structure of the antifungal substance was determined to be rhamnolipid B by mass and NMR spectral analyses. The antifungal biosurfactant showed a potent in vivo antifungal activity against gray mold and late blight on tomato plants. In addition, rhamnolipid B inhibited mycelial growth of B. cinerea causing tomato gray mold and zoospore germination and mycelial growth of P. infestans causing tomato late blight. Pseudomonas sp. SG3 producing rhamnolipid B could be used as a new biocontrol agent for the control of plant diseases occurring on tomato plants.

• Tunnel and Underground Space
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• v.19 no.5
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• pp.432-439
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• 2009

For more reliable estimation of seismic source, attenuation properties and dynamic ground property, site amplification function should be considered. Among various estimation methods, this study used the Nakamura's method (1989) for estimating site amplification characteristics. This method was originally applied to the surface waves of background noise and therefore there are some limitations in applying to general wave energy. However, recently this method has been extended and applied to the S wave energy successfully. This study applied the method to S wave and Coda wave energy, which is equivalent to the backscattered S wave energy. We used more than 60 observed ground motions from 5 earthquakes which were occurred recently, with magnitude range from 3.6 to 5.1. Each station showed characteristic site amplification property in low-, high- and resonance frequencies. Some of the stations showed as high as 4 times of site amplification in the range of specific frequencies, which may imply abnormal small scale geologic strata below the station or development of various trapped modes in the basin structure. Moreover, removal of site amplification can give us more reliable seismic source and attenuation parameters, addition to the seismic hazard estimation.

• Journal of the Korean Society for Nondestructive Testing
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• v.26 no.2
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• pp.77-83
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• 2006

Ultrasonic nonlinearity has been considered as a solution for the detection of microcracks or interfacial delamination in a layered structure. The distinguished phenomenon in nonlinear ultrasonics is the generation of higher-order harmonic waves during the propagation. Therefore, in order to quantify the nonlinearity, the conventional method measures a parameter defined as the amplitude ratio of a second-order harmonic component and a fundamental frequency component included in the propagated ultrasonic wave signal. However, its application In field inspection is not easy at the present stage because no standard methodology has yet been made to accurately estimate this parameter. Thus, the aim of this paper is to propose an advanced signal processing technique for the precise estimation of a nonlinear ultrasonic parameter, which is based on power spectral and bispectral analysis. The method of estimating power spectrum and bispectrum of the pulse-like ultrasonic wave signal used in the commercial SAM (scanning acoustic microscopy) equipment is especially considered in this study The usefulness of the proposed method Is confirmed by experiments for a Newton ring with a continuous air gap between two glasses and a real semiconductor sample with local delaminations. The results show that the nonlinear parameter obtained tv the proposed method had a good correlation with the delamination.

• Applied Biological Chemistry
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• v.23 no.2
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• pp.123-130
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• 1980

Thiamine derivatives of thiamine tetrahydrofurfuryl disulfide (TTFD) and thiamine propyl disulfide (TPD) were obtained in a good yield and purity by use of thiothiamine $(SB_1)$ and also described in details for the preparing sodium tetrahydrofurfuryl thiosulphate (Bunte's salt) with and without KI. The optimum reaction conditions for the preparation of TTFD was set in which the Box-Wilson plan was applied. The reaction conditions are as follows; 1. pH value of aqueous solution of thiol type of $B_1{\cdot}HCl;12.09$ 2. Quantity of Bunte's salt (to $B_1{\cdot}HCl\;20G$); 35.01g 3. Reaction temperature; $15.59^{\circ}C$ Studies on ultraviolet absorption were made at various pH and showed that the absorption maxima are shifted with change of pH. The absorption maxima are at $244{\sim}246nm,\;234{\sim}235nm$ in TTFD, and $245{\sim}246nm,\;233{\sim}235nm$ in TPD. The structure was proved by the infrared spectral evidence. Quantitative determination was studied.

• Journal of Sensor Science and Technology
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• v.9 no.5
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• pp.341-349
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• 2000

CsI(Br) single crystals doped with 1, 3, 5 or 10 mole% $Br^-$ ions, as an activator, were grown by Czochralski method. The lattice structure of grown CsI(Br) single crystal was bcc and its lattice constant was $4.568\;{\AA}$. The absorption edge of the CsI(Br) single crystals was observed at 243 nm. The spectral range of the luminescence excited by 243 nm of wavelength was $300{\sim}600\;nm$, and its peak emission appeared at 440 nm. The luminescence intensity was maximum when CsI(Br) was doped with 3 mole % $Br^-$ ions. The energy resolutions of the CsI(Br) scintillator doped with 3 mole % $Br^-$ ions were 15.0% for $^{137}Cs$(662 keV), 13.1% for $^{54}Mn$(835 keV), and 18.0% and 6.3% for $^{22}Na$(511 keV and 1275 keV), respectively. The decay curves had fast and slow components, and the fast component was about 41 ns independent on the concentration of the $Br^-$ ions. The time resolution of CsI(Br) scintillators decreased with increasing of the concentration of $Br^-$ ions.

• Applied Chemistry for Engineering
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• v.18 no.1
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• pp.77-83
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• 2007

It is essential that second order nonlinear optical materials have low optical propagation losses in the wavelengths of second harmonic generation for practical applications in waveguides. Three dipolar chromophores substituted with nitro, cyano, and alkyl sulfone as an electron withdrawing group were prepared. The UV-Vis absorption spectra of the cyano and alkylsulfone chromophores showed a blue-shift compared to the nitro chromophore. The introduction of oxadiazole segment in the chromophore structure led to similar spectral shift. The blue-shift can produce low optical loses at second harmonics. The chromophores were successfully attached to a polyimide, yielding side chain polymers. The nonlinear optical property of the prepared optical polymers was determined by measuring electro-optic coefficient at 1.55 mm. The polymers exhibited high glass transition temperature of over $185^{\circ}C$ and thermal stability to $300^{\circ}C$ through differential scanning calorimeter analysis and thermal gravimetric analysis.

• The Korean Journal of Food And Nutrition
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• v.27 no.3
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• pp.532-537
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• 2014

Cortex Phellodendri (CP) is derived from the dried bark of Phellodendron amurense. It has been widely used as a drug in traditional Korea medicine for treating diarrhea, jaundice, swelling pains in the knees and feet, urinary tract infections, and infections of the body surface. Many analytical methods have been used to study oriental herbal medicines, such as thin-layer chromatography, column liquid chromatography, and high performance liquid chromatography (HPLC). In this study, preparative centrifugal partition chromatography (CPC) was successfully carried out in order to separate pure compounds from a CP methanol extract. The optimum two-phase CPC solvent system was composed of n-butanol: acetic acid: water (4:1:5 v/v/v). The flow rate of the mobile phase was 3 mL/min in ascending mode with rotation at 1,000 rpm. The CPC-separated fraction and purification procedures were carried out by preparatory HPLC. The $^1H$ NMR spectrum revealed that the resonances at ${\delta}$ 4.10 and 4.20 ppm corresponded to three protons ($-OCH_3$), whereas those at ${\delta}$ 6.10 ppm corresponded to two protons ($-OCH_2O-$). Further, two aromatic protons (H-11 and H-12) conveys a doublet-doublet pattern. The H-11 doublet and H-12 doublet appear at ${\delta}$ 7.98 and 8.11, respectively. The $^{13}C$ NMR. spectrum showed a tetrasubstituted with a methylenedioxy group at C2 and C3, and two methoxy groups at C9 and C10. The chemical structure of the berberine was identified by $^1H$, $^{13}C$-nuclear magnetic resonance and electrospray ionization-mass spectroscopy spectral data analysis.

• Korean Journal of Food Science and Technology
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• v.30 no.1
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• pp.230-236
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• 1998

We have screened total 32 herbal drugs to find the highest activity against human cariogenic enzyme, glucosyltransferase (GTase) from the extracts of Magnoliae bark. The extracts were separated into three phases, i. e. water, n-butanol and ethylacetate according to their solvent polarity. Among them, ethylacetate fraction had approximately more than 70% of total activities, and the active principle was further isolated by prep. HPLC following silicagel column chromatography to yield single compound as white powder. The chemical structure of the compound was finally elucidated to be 4,4'-dihydroxy-3,3'-dimethoxylignan from the spectral data of FAB-MS. $^1H-\;and\;^{13}C-NMR$ spectrometries. The compound was also shown to have relatively strong antibacterial activity against ten types of cariogenic oral bacteria and one kind of Actinomyces sp.

• Geophysics and Geophysical Exploration
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• v.22 no.4
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• pp.210-224
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• 2019

Surface wave (SW) surveys, which have been applied to numerous application fields ranging from micro-scale ultrasonic analysis to geological scale analysis, are widely used to monitor near-surface stability. The survey method is basically made through analysis on dispersion of SW propagating along the earth surface, in order to delineate shear velocity structure of subsurface. SW survey data are inverted with assuming one-dimensional (1D) layered-earth in order to recover shear wave velocities of each layer, after being analyzed to make the dispersion curve that shows phase velocity of SW with respect to frequency. This study reviews surface wave surveys with explaining the basic theory including the characteristics of dispersion and the procedure of general data processing. Even though surface wave surveys can be categorized into active and passive methods, this paper focuses only on active surface wave methods which includes continuous SW (CSW), spectral analysis of SW (SASW) and multichannel analysis of SW (MASW). Passive method will be reviewed in the subsequent paper.

• Korean Journal of Optics and Photonics
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• v.19 no.1
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• pp.20-24
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• 2008

A color filter was proposed and demonstrated by incorporating a subwavelength patterned 1-dimensional grating in poly silicon. It was produced by employing the laser interference lithography method, providing much wider effective area compared to the conventional e-beam lithography. A $SiO_2$ layer was introduced on top of the silicon grating layer as a mask for the etching of the silicon, facilitating the etching of the silicon layer. It was theoretically found that the selectivity of the filter was also improved thanks to the oxide layer. The parameters for the designed device include the grating pitch of 450 nm, the grating height of 100 nm and the oxide-layer height of 200 nm. As for the fabricated filter, the spectral pass band corresponded to the blue color centered at 470 nm and the peak transmission was about 40%. Within the effective area of $3{\times}3mm^2$, the variation in the relative transmission efficiency and in the center wavelength was less than 10% and 2 nm respectively. Finally, the influence of the angle of the incident beam upon the transfer characteristics of the device was investigated in terms of the rate of the relative transmission efficiency, which was found to be equivalent to 1.5%/degree.