• Journal of Plant Biology
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• v.11 no.1
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• pp.7-14
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• 1968

Buckwheat seeds produced in previous year (1965) in an experimental pot culture in which nine levels of P-32 ranging from $1.4{\times}10-4 to 3.3{\times}104$\mu$c/pot(as of 27 July 1965)$, with the same specific activity, had been applied to the corresponding pots respectively, were used this year(1996) in water and soil culture as well as in germination test to investigate the feature and extent of possible residual effects of P-32 incorporated upon germination and plant growth, and the following results were obtained: 1. Under the given experimental conditions both stimulative and inhibitory effects of radiation were observed. 2. The germination rate of the seeds was lower at the higher level of P-32 aplication ranging from $3.0{\times}103 to 3.3{\times}104$\mu$c/pot$ and higher at 0.2 $\mu$c P/pot than the control. 3. Among the seeds produced at the higher level of application about 80% was failed to germinate, owing to the radiation injury. The remaining 80% was failed to germinate, owing to the radiation injury. The remaining 80% survived the damaging effect and showed vigorous growth and increased yield. The latter group of seeds thus proved themselves to be more radioresistant than the former. 4. The survived seeds produced later more straw and root on dry weight basis. The higher the level of P-32 applied, the stronger the stimulative effect showed in vegetative growth. 5. No radiation effect on linear growth of the plants was observed in the soil culture. 6. The dry weight of straw produced showed little difference at the moderate range compared with that of control in the soil culture. At high level of application, i.e. over $\mu$c P32/pot, however, the production was increased by 12-37% of control. 7. As for the dry weight of root harvested, the P-32 treatment over 24$\mu$c P32/pot produced 82-155% more than the control, whereas little difference was observed under 2.2$\mu$c P32/pot. 8. The seed production increased in general by the P-32 treatments. Particularly at the moderate level of application the rate of increase amounted to 70% of the control. 9. Those individual plants which survived damaging effects of radiation at the germinating stage shwoed remarkable stimulative effects both in vegetative and in reproductive stage of growth.

• Food Science and Preservation
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• v.9 no.4
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• pp.429-433
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• 2002

The D-xylulokinase from Candida sp. L-16 was purified through a sequence of ammonium sulfate fractionation, DEAE-cellulose chromatography, Sephadex G-100 and Sephadex G-200 gel filtration. The specific activity of the purified Dxylulokinase was increased to 23.2 fold and the yield was 11.2%. The enzyme was showed to be a single protein band by SDS-PAGE. The molecular weight of the enzyme was 150,000 dalton, this enzyme was identified to be a dimer with two subunits. The optimum conditions of the enzyme were pH 8.0 and 40$\^{C}$, respectively. The enzyme was relatively stable between pH 7.0 to pH 9.0, but it was unstable over 30$\^{C}$. The enzyme showed substrate specificity on D-xylulose, D-arabinose and D-ribose, Km value and Vmax for D-xylulose were 0.042 mM and 117 units/ml, respectively. The activation energy of the enzyme was 4.75 Kcal/mol. The one was inhibited by metabolic intermediates such as 6-phosphogluconic acid, 2-keto-gluconic acid. The enzyme was activated by EDTA and thiol compounds such as cysteine-HCI, DTT and glutathione.

• Journal of Radiation Protection and Research
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• v.34 no.4
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• pp.190-194
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• 2009

Gamma spectral results for macroalgae samples taken from the environment of Ulchin nuclear power plants in Korea (east coast), showed 766 keV peaks, which were identified as $^{95}Nb$ by several research institutes. After the enhancement of liquid radioactive waste disposal facility at Ulchin NPP site, the $^{95}Nb$ amount in the liquid radioactive waste outflow has drastically reduced, but the expected reduction in $^{95}Nb$ specific activity from environmental samples did not actually show up on gamma spectroscopy. Detailed re-investigation revealed that along with 766 keV peak, other peaks (63, 92 and 1001 keV) from $^{234}Th-^{234}mPa$ decay series were also detected on spectroscopy, and that the measured half lives of the four peaks were very close to known half life of $^{234}Th-^{234}mPa$ decay series, which is 24.1 day. The measured gamma yield ratios of 766 keV peak to 1001 peak were very close to known ratio 0.35 for $^{234}mPa$. It is concluded that 766 keV peaks on gamma spectroscopy of Ulchin NPP environmental samples were mainly from $^{234}mPa$, which is one of naturally occurring radionuclides.

• Korean Journal of Food Science and Technology
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• v.31 no.6
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• pp.1635-1641
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• 1999

Bacillus subtilis PCA20-3 was isolated from meju and was found to produce a protease. The strain produced the maximum amount of enzyme in the medium containing soytone (0.2%), soluble starch (2%), $(NH_4)_2SO_4\;(0.1%),\;CaCl_2(0.1%),\;yeast\;extract\;(0.01%),\;K_2HPO_4\;(0.1%),\;and\;KH_2PO_4\;(0.1%)$. Protease was first concentrated by ammonium sulfate (80% saturation, w/v) precipitation of culture supernatant. Then the enzyme was purified by column chromatography using CM Sephadex C-50. The collected proteins were rechromatographed using Sephadex G-100 gel filtration column. The fraction with protease active from Sephadex G-100 gel chromatography was found to be pure when examined by SDS-polyacrylamide gel electrophoresis and YMC-pak reverse phase chromatography. Specific activity, yield and purity were 76 U/mg. 2.7%, and 7.6 fold, respectively. The molecular weight of the enzyme was estimated to be 31.5 kDa by SDS-PAGE. The number of amino acids calculated from molecular weight was evaluated about 321 residues. N-terminal sequence of the enzyme was $Val^1-Pro^2-Tyr^3-Gly^4-Val^5-Ser^6-Gln^7-Gly^8-Lys^9-Ala^{10}$.

• Journal of Life Science
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• v.25 no.4
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• pp.425-432
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• 2015

This study was performed to develop high-value-added biomaterials for health and beauty products. Extracts of ethanol and hot water and their subsequent organic solvent fractions were prepared from Lees of Wookukseng (LW), a commercialized Korean traditional rice wine. We investigated their activities on blood coagulation, platelet aggregation, hemolysis against human red blood cells (hRBCs), and anti-oxidation. The water content, pH and brix of the LW were 80.3%, 3.94 and 13.0°, respectively. The yield of ethanol extraction (6.62%) was 3.15 times higher than that of hot-water extraction (2.1%), and the ethyacetate fraction (EAF) of ethanol extract showed the highest content of total polyphenol (128 mg/g) among the various fractions. In anticoagulation activity assay, the EAF of ethanol extract showed a 15-fold extension in TT, PT, and aPTT, indicating that the EAFs contain various inhibitory substances against thrombin, prothrombin and coagulation factors. In anti-platelet aggregation activity assay, the butanol fraction and water residue of ethanol extract showed significant inhibition activity. The activities were comparable to aspirin, a commercial anti-thrombosis agent. The above extracts and fractions did not show hemolysis activity against hRBC up to 5 mg/ml, and had radical scavenging activity against DPPH anion, ABTS cation and nitrite. Our results suggest that the active fractions prepared from LW, which has no specific usage until now, have a high potential as novel resources for anti-thrombosis agents.

• KSBB Journal
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• v.21 no.2
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• pp.133-139
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• 2006

In 'solid-phase' PEGylation, the conjugation reaction occurs as the proteins are attached to a solid matrix, and thus it can have distinct advantages over the conventional, solution-phase process. We report a case study: rhIFN-${\alpha}$-2a was first adsorbed to cation exchange resin and then N-terminally PEGylated by aldehyde mPEG of 5, 10, and 20 kD through reductive alkylation. After the PEGylation, salt gradient elution efficiently recovered the mono-PEGylate in a purified form from the unwanted species such as unmodified IFN, unreacted PEG, and others. The mono-PEGylation and its purification were integrated in a single chromatographic step. Depending on the molecular weight of the mPEG aldehyde used, the mono-PEGylation yield ranged 50-64%. We could overcome the major problems of random, or uncontrollable, multi-PEGylation and the post-PEGylation purification difficulties associated with the solution-phase process. N-terminal sequencing and MALDI-TOF MS confirmed that a PEG molecule was conjugated only to the N-terminus. Compared with the unmodified IFN, the mono-PEGylate showed the reduced anti-viral activity as measured by the cell proliferation assay. The bioactivity was reduced more as the higher molecular weight PEG was conjugated. Immunoreactivity, evaluated indirectly by antibody binding activity using a surface plasmon resonance biosensor, also decreased. Nevertheless, trypsin resistance as well as thermal stability was considerably improved.

• Journal of the Korean association of regional geographers
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• v.15 no.5
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• pp.671-687
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• 2009

This paper aims to link the development strategy of a local government(Guri-si, Kyunggi-do) to a spatial decision support system that is in the early stages of implementation and testing. Spatial Decision for a local governmental authority typically involves multiple stakeholders. To any specific regional problem, stakeholders often have various levels of knowledge and experiences about it, that yield conflicting views. In this point of view, the enhanced access to information can bring the decision process to solve the problem throughout more public participation. Recently, Urban development study was required to improve the decision making of public participation form. This study aim to investigate an analysis local government of Guri-si, can be supported as the suggestion of decision making system. Guri-si has been argued that it would improve citizen's life quality intent theme 'by historical tourism in general dimension' promote region identity, environment friendly and transportational strength, by reflecting various plan of urban development and it executed strategy. There are increasing interest in citizen participation and practical using of policy decision making to respond to uncertain environment and systematic exploration of local government. Focused on the contents of public participation decision making, it is important to investigate how to analysis urban development strategy and geographic information relationship for useful. The paper concludes with a discussion of current issues encountered during development and implementation of spatial decision making(or support) system for the local administrative authority.

• Applied Biological Chemistry
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• v.38 no.3
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• pp.191-195
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• 1995

Production of a high viscosity exoploysaccharide, methylan, by Methylobacterium organophilum from methanol was carried out in fed-batch cultures and the rheological properties of methylan fermentation broth were studied. Bacterial biomass showed little influence on viscosity, but the accumulation of methylan caused the increase of viscosity. With proceeding fermention, the viscosity at the same concentration of methylan was significantly increased and methylan solution showed slightly higher pseudoplasticity. The composition changes of methylan were investigated at various fermentation times. Contents of total sugar, reducing sugar and methylan were decreased but contents of acids(pyruvic acid, uronic acid and acetic acid) were increased with the culture time. It was considered that the increased content of acids resulted in the increase of the hyrodynamic domain in the solution due to charge repulsion. Consequently, the solution viscosity increased in propotion to the acids contents of methylan. Cell growth and methylan production were severely decreased by the limitation of dissolved oxygen. However, the cellular activity for methylan production was almost constant regardless of the level of dissolved oxygen. As a result, the high speed of agitation increased the methylan production, the specific production rate of methylan, and the methylan yield of the cell.

• Korean Journal of Agricultural and Forest Meteorology
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• v.14 no.2
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• pp.79-89
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• 2012

This study was conducted to investigate the chlorophyll contents, photosynthetic characteristics, chlorophyll fluorescence, and growth performance of Parasenecio firmus under changing light environment. Parasenecio firmus was grown under non-treated (full sunlight) and three different shading conditions (88~93%, 65~75% and 45%~55% of full sunlight) for the experiment. Total chlorophyll content, photochemical efficiency (Fv/Fm), T/R ratio, specific leaf area (SLA), leaf area ratio (LAR), and leaf weight ratio (LWR) were increased with increasing shading level, but decreased dark respiration. Therefore, light absorption and light utilization efficiency were improved under the low intensity light. Plants under 65~75% of full sunlight had best maximum photosynthetic rate and net apparent quantum yield in May. On the other hand, the non-treated plants had lower maximum photosynthetic rate, photochemical efficiency, and chlorophyll content than the treated ones. Parasenecio firmus considered to be a sciophyte, is fairly sensitive to high intensity light. If 88-93% of full sunlight lasts for a long period, photosynthetic capacity will be sharply decreased, though limiting light. These results suggest that growth of Parasenecio firmus adapted to 65~75% of full sunlight.

• Journal of the Korean Society of Groundwater Environment
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• v.6 no.3
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• pp.152-157
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• 1999

Oil leaked from underwound storage tanks and leachate from sanitary landfills have been known as contaminant sources of the high-quality groundwater resources. The mobility of contaminants in the aquifer largely depends on the groundwater flow and the determination of associated hydraulic parameters is essential for a proper remediation of contaminated grnundwater. This study aimed at determining an optimum set of hydraulic parameters for an unconfined sandy aquifer of a laboratory scale through comparison of various methods. Results showed that the specific yield obtained from gravity drainage experiment was an average of 0.20 with minor variations in aquifer depths. and the permeabilities obtained from Dupuit approximation and slug test gave similar values of 5.33 cm/min and 5.85 cm/min but the constant head method gave 0.17 cm/min, which is much ion than the other methods. This experimental evidence reveals that the permeability of the unconfined sandy aquifer could be accurately determined by Dupuit assumption or slug tut rather than by constant head method conducted for a disturbed separate soil column.