• 한국미생물·생명공학회지
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• 제20권4호
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• pp.483-490
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• 1992

수계 환경에서 일어나는 유전자의 전이행방을 이해하기 위하여, conjugation에 의하여 전이되는 kanamycin 내성 ($Km^r$) 유전자에 대하여 DNA probe를 이용하여 Southern hybridization 방법으로 추적하였다. 자연계로부터 분리한 $Km^r$ 세균과 $Km^r$ 유전자를 유전자 조작기법으로 변형시킨 GMM 균주들을 donor로 하여 conjugation을 했을 때, $Km^r$ 유전자는 자연계 분리 균주에서보다 수질환경에 관계없이 10~00배 잘 전이되었다. LB 배지에서 GMM 균주의 $Km^r$ 유전자가 전이된 conjugant에서는 새로 생성되는 plasmid가 많이 나타났고 AW와 FW에서는 conjugation 시간에 따라 plasmid의 재배열 현상이 다양하였다. LB에서 얻은 conjugant들의 plasmid에 대하여 $Km^r$ DNA probe로 Southern analysis를 한 결과, plasmid의 재배열이 다양함에도 불구하고 conjugant들의 $Km^r$ plasmid는 donor에서와 같은 위치에서 hybridization signal이 나타났다. 그러나 AW에서 50시간 conjugation시켰을 때 DKI의 pDK101과 DKC601의 pDT529, 그리고 AW에서 30시간 conjugation 시켰을 때 DKC600의 pDK101은 전혀 나타나지 않고, 소실되었다. 또 전이된 $Km^r$ plasmid의 크기는 AW와 FW의 수질에 따라 약간 변화되어 나타났다. 그러므로 DNA probe에 의한 Southern hybridization 방법은 수질환경에서 특정 유전자의 전이행방을 추적하는데 매우 유용하다고 판단된다.

• 공업화학
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• 제27권2호
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• pp.210-216
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• 2016

본 연구에서는 무전해 니켈 도금 시간을 달리하여 제조한 자동차 시트용 탄소섬유 발열체의 발열 및 전기적 특성에 관하여 고찰하였다. 무전해 니켈 도금된 탄소섬유의 비저항 및 비열은 4-point probe method 및 differential scanning calorimetry (DSC)를 이용하여 분석하였으며, 표면 형상 및 표면 온도는 scanning electron microscope (SEM) 및 열화상 카메라를 이용하여 관찰하였다. 실험 결과, 도금시간의 증가에 따라 니켈 도금 두께 및 표면 온도는 증가하였으며, 반면에 비열 및 비저항은 도금시간이 증가함에 따라 감소하였다. 결과적으로 무전해 니켈 도금은 자동차 시트용 탄소섬유 발열체의 저항 발열 및 전기적 특성을 크게 향상시키는 것으로 판단된다.

• 동력기계공학회지
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• 제15권2호
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• pp.13-18
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• 2011

This thesis is about the result of conducting a specific experiment for the development of noncontact vibration displacement sensor for measuring the spindle vibration that is used for conditional monitoring of machinery. One should be careful when using the eddy current type displacement sensor because the sensitivity of it is different according to the quality of the material. While the probe used for nondestructive inspection adopts the effect of transmitting the material by using the high frequency domain, the eddy current type displacement sensor uses the lower frequency of around 1MHz. Also, while the nondestructive probe uses the method of enhancing output by using the resonance zone, the vibration displacement sensor utilizes the stable zone by avoiding the resonance zone. Since the oscillator of the converter uses the "L" element as Probe, its characteristic changes with the variation of a relevant impedance. In other words, if the length of Probe's Cable gets extended (Impedance increase), the sensitivity declines accordingly. The effect of surrounding temperature was small, but the influence of the quality of Sensor Coil used was high. Moreover, following an experimental demonstration of the phenomenon where the sensitivity decreases as the frequency of the tested material increases from a frequency response test, the maximum frequency that could be measured was approximately 1KHz. It was noted that the degree of precision could be maintained by using the gap of the probe in the linear zone at the installation site.

• 대한의생명과학회지
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• 제25권4호
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• pp.407-416
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• 2019

Recent years have seen an increase in the incidence of candidiasis caused by non-albicans Candida (NAC) species. In fact, C. glabrata is now second only to C. albicans as the most common cause of invasive candidiasis. Therefore, the rapid genotyping specifically for C. glabrata is required for early diagnosis and treatment of candidiasis. A number of genotyping assays have been developed to differentiate C. glabrata sequence types (STs), but they have several limitations. In the previous study, multi-locus sequence typing (MLST) has performed with a total of 101 C. glabrata clinical isolates to analyze the prevalent C. glabrata STs in Korea. A total of 11 different C. glabrata STs were identified and, among them, ST-138 was the most commonly classified. Thus, a novel probe-based quantitative PCR (qPCR) assay was developed and evaluated for rapid and accurate identification of the predominant C. glabrata ST-138 in Korea. Two primer pairs and hybridization probe sets were designed for the amplification of internal transcribed spacer 1 (ITS1) region and TRP1 gene. Analytical sensitivity of the probe-based qPCR assay was 100 ng to 10 pg and 100 ng to 100 pg (per 1 μL), which target ITS1 region and TRP1 gene, respectively. This assay did not react with any other Candida species and bacteria except C. glabrata. Of the 101 clinical isolates, 99 cases (98%) were concordant with MLST results. This novel probe-based qPCR assay proved to be rapid, sensitive, highly specific, reproducible, and cost-effective than other genotyping assay for C. glabrata ST-138 identification.

• 한국정보통신학회논문지
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• 제26권4호
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• pp.612-623
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• 2022

실시간으로 유동인구를 계측하는 기술은 다양한 산업 분야에서 공간 밀집도에 대한 통찰력을 제공하여 더 좋은 서비스 환경을 만들어준다. 이에 따라 여러 기업과 학계에서는 특정 공간의 유동인구 데이터를 계측하기 위해 오랫동안 다양한 연구를 시도해왔으며, 최근에는 스마트시티와 디지털트윈의 일환으로 Wi-Fi 신호를 활용한 유동인구 분석 사업화가 더욱 활발한 추세이다. 본 논문에서는 사람들이 소지한 스마트폰으로부터 수집되는 MAC-address 값 기반의 유동인구 추정 방법을 제시하는데, 추정값의 정확도를 분석하기 위해 Real MAC-address와 Random MAC-address 값을 구분한 뒤, Real MAC-address가 추출된 실제 스마트폰 기기 수와 CCTV 화면에 집계된 사람 수를 비교하는 실험을 진행한다. 그 결과 두 데이터 간의 유사한 상관 계수가 나타났다. 이러한 결과에 근거하여 MAC-address 분류를 통한 Wi-Fi Probe Request 기반 유동인구 분석 방법을 제시한다.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2021년도 추계학술대회
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• pp.24-34
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• 2021

실시간으로 유동인구를 계측하는 기술은 다양한 산업 분야에서 공간 밀집도에 대한 통찰력을 제공하여 더 좋은 서비스 환경을 만들어준다. 이에 따라 여러 기업과 학계에서는 특정 공간의 유동인구 데이터를 계측하기 위해 오랫동안 다양한 연구를 시도해왔으며, 최근에는 스마트시티와 디지털트윈의 일환으로 Wi-Fi 신호를 활용한 유동인구 분석 사업화가 더욱 활발한 추세이다. 본 논문에서는 유동인구 데이터를 활용하는 일반 수요자(비전문가)의 관점에서 유동인구 계측 시스템에 대해 쉽게 이해할 수 있도록 설명한다. 구체적으로는 사람들이 소지한 스마트폰으로부터 수집되는 MAC-address 값 기반의 유동인구 추정 방법을 제시하는데, 추정값의 정확도를 분석하기 위해 Real MAC-address와 Random MAC-address 값을 구분한 뒤, Real MAC-address가 추출된 실제 스마트폰 기기 수와 CCTV 화면에 집계된 사람 수를 비교하는 실험을 진행한다. 그 결과 두 데이터 간의 유사한 상관 계수가 나타났다. 이러한 결과에 근거하여 MAC-address 분류를 통한 Wi-Fi Probe Request 기반 유동인구 분석 방법을 제시한다.

• Journal of Microbiology and Biotechnology
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• 제17권6호
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• pp.993-1001
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• 2007

The technique of serological analysis of antigens by recombinant cDNA expression library (SEREX) uses autologous patient sera as a screening probe to isolate tumor-associated antigens for various tumor types. Isolation of tumor-associated antigens that are specifically reactive with patient sera, but not with normal sera, is important to avoid false-positive and autoimmunogenic antigens for the cancer immunotherapy. Here, we describe a selection methodology to isolate patient sera-specific antigens from a yeast surface-expressed cDNA library constructed from 15 patient lung tissues with non-small cell lung cancer (NSCLC). Several rounds of positive selection using patient sera alone as a screening probe isolated clones exhibiting comparable reactivity with both patient and normal sera. However, the combination of negative selection with allogeneic normal sera to remove antigens reactive with normal sera and subsequent positive selection with patient sera efficiently enriched patient sera-specific antigens. Using the selection methodology described here, we isolated 3 known and 5 unknown proteins, which have not been isolated previously, but and potentially associated with NSCLC.

• Fisheries and Aquatic Sciences
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• 제3권1호
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• pp.64-70
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• 2000

DNA probes for the l6S rRNA have been designed for the detection of Edwardsiella tarda. In order to accomplish this purpose, the l6S rRNA gene from E. tarda has been cloned and sequenced. Two highly feasible oligonucleotide probe sites have been determined by the database analysis programs presented by PCGENE and BLAST. These two probes have been evaluated by slot blot hybridization analysis. Hetero- and homo-trimeric templates have been synthesized using these two probe sites. The templates have been further multimerized by PCR to generate between 150 and 300 bp long DIG-11-dUTP labeled probes. Unlike 3' end labeled oligonucleotide probes or templates, multimerized probes showed no cross­hybridization in the given experimental condition. Furthermore, a significant increase in sensitivity has been observed with these probes. This method, we presented here, may be useful for the designing of probes for the detection of other fish pathogenic microorganisms also.

• Journal of electromagnetic engineering and science
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• 제15권3호
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• pp.181-184
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• 2015

Evaluation of radiating radiofrequency fields from hand-held and body-mounted wireless communication devices to human bodies are conducted by measuring the specific absorption rate (SAR). The uncertainty of system validation and probe calibration in SAR measurement depend on the variation of RF power used for the validation and calibration. RF input power for system validation or probe calibration is controlled manually during the test process of the existing systems in the laboratories. Consequently, a long time is required to reach the stable power needed for testing that will cause less uncertainty. The standard uncertainty due to this power drift is typically 2.89%, which can be obtained by applying IEC 62209 in a normal operating condition. The principle of the Automatic Input Power Level Control System (AIPLC), which controls the equipment by a program that maintains a stable input power level, is suggested in this paper. The power drift is reduced to less than ${\pm}1.16dB$ by AIPLC, which reduces the standard uncertainty of power drift to 0.67%.

• 한국식물병리학회지
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• 제14권2호
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• pp.177-183
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• 1998

A DNA fragment which is involved in tolassin production was cloned to obtain a molecular marker of Pseudomonas tolaasii, a casual agent of bacterial brown blotch disease of oyster mushroom (Pleurotus ostreatus). Tolaasin is a lipodepsipeptide toxin and known as a primary disease determinant of the P. tolaasii. It is responsible for formation of white line in agar when P. tolaasii were cultured against white line reacting organisms (WLROs). White line negative mutants (WL-) were generated by conjugation between rifampicin resistant strain of P. tolaasii and E. coli carrying suicidal plasmid pSUP2021 : : Tn5. The ability of tolaasin production of the WL- mutants was examined by hemolysis test, pathogenicity test, and high pressure liquid chromatography (HPLC) analysis of culture filtrate. All of the WL- mutants were lost the ability of tolaasin production (Tol-). Genomic library of the Tol- mutant was constructed in pLAFR3 and the cosmid clone containing Tn5 was selected. DNA fragment fro franking region of Tn5 was cloned from the plasmid and used as a probe in Southern blot. DNA-DNA hybridization with the probe to total DNA from group of bacteria ecologically similar to P. tolaasii including WLORs, fluorescent Pseudomonads isolated from oyster mushroom, P. agarici, P. gingeri, and some of other species of Psedomonas showed that some of the tested bacteria do not have any hybridized band and others have bands sowing RFLP. The cloned DNA fragment or its nucleotide sequence will be useful in detection and identification of the P. tolaasii.