• Horticultural Science & Technology
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• v.34 no.4
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• pp.607-615
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• 2016

This research was conducted to investigate the influence of different kinds of acids added to irrigation water containing high levels of bicarbonate on the growth and daughter plant production during the propagation of 'Seolhyang' strawberry. Fertigation solution was prepared with equal concentrations of essential nutrients as found in Hoagland solution, plus $240mg{\cdot}L^{-1}\;HCO_3{^-}$. The concentrations of $HCO_3{^-}$ in the treatment solution were decreased to $60mg{\cdot}L^{-1}$ by the addition of $HNO_3$, $H_3PO_4$, $H_2SO_4$, $HNO_3+H_3PO_4$, or salicylic acid. The mother plants transplanted to raised beds were treated with a specific treatment solution for 126 days and growth and daughter plant productions were monitored. The fresh weight of mother plants in the -control treatment ($240mg{\cdot}L^{-1}\;HCO_3{^-}$) was significantly lower than those in acid containing treatments. The number of daughter plants produced per mother plant was 13 in the -control treatment, but 19.4, 20.1, 18.6, 22.4, and 18.9 in the treatments of $HNO_3$, $H_3PO_4$, $H_2SO_4$, $HNO_3+H_3PO_4$ and salicylic acid, respectively. The substrate pHs after 126 days of treatment were 8.2 and 7.3 in the -control and salicylic acid treatments, respectively, but 5.5, 5.4, 5.3, and 5.5 in the treatments of $HNO_3$, $H_3PO_4$, $H_2SO_4$, and $HNO_3+H_3PO_4$ treatments, respectively. The tissue Ca and Mg contents of mother plants were significantly higher in +control ($90mg{\cdot}L^{-1}\;HCO_3{^-}$) and various acid blending treatments compared to the -control treatment. By contrast, Ca and Mg contents were not significantly different among treatments with acids. The Fe, Mn, Zn, and Cu contents in plant tissue of -control were also significantly lower than those in all other treatments.

• Journal of Plant Biotechnology
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• v.42 no.3
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• pp.204-214
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• 2015

SHI-RELATED SEQUENCE (SRS) genes are plant-specific transcription factors that contain a zinc-binding RING finger motif, which play a critical role in plant growth and development. Among Brassica rapa SRS genes, BrSRS7 and BrLRP1 genes, isolated from shoot apical regions are important regulators of plant growth and development. In order to explore the function of BrSRS genes in horticultural plant growth and development, two constructs containing BrSRS7 and BrLRP1 under the control of a cauliflower mosaic virus 35S promoter were introduced into petunia by Agrobacterium-mediated transformation. The resulting transgenic plants were dwarf and compact plants with reduced plant height and diameter. Additionally, these transgenic plants had upward-curled leaves of narrow width and short internodes. Interestingly, the flower shapes of petunia were different among transgenic plants harboring different kinds of SRS genes. These phenotypes were stably inherited through generations $T_2$ and $T_3$. Semi-quantitative RT-PCR analyses of transgenic plants revealed that BrSRS7 and BrLRP1 regulate expression of gibberellin (GA)- and auxinrelated genes, PtAGL15- and PtIAMT1-related, involved in shoot morphogenesis. These results indicate that the overexpression of BrSRS7 and BrLRP1 genes suppressed the growth and development of petunia by regulating expression of GA- and auxin-related genes. From these data, we deduce that BrSRS7 and BrLRP1 genes play an important role in the regulation of plant growth and development in petunia. These findings suggest that transformation with the BrSRS genes can be applied to other species as a tool for growth retardation and modification of plant forms.

• Journal of Korean Society of Forest Science
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• v.106 no.2
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• pp.130-149
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• 2017

The subalpine zone which is vulnerable to climate change could be functioning as a habitat for northern plant species especially in the Korean peninsula. In this study, classification of phytosociological community type and investigation on flora of vascular plants of Daecheongbong area in Mt. Seorak were carried out in order to provide a basic information for an ecological restoration and management of the vegetation through community ecological approach. Vegetation data were collected from 31 of quadrates from May to October, 2016. We analyzed vegetation types with phytosociological method, importance value with Curtis & McIntosh method, CCA using PC-ORD program tool, and flora of vascular plants. As a result, the forest vegetation was classified into Abies nephrolepis community. The community was divided 3 groups such as Pinus pumila group, Rhododendron brachycarpum group and Angelica decursiva group. Therefore, the vegetation had 3 kinds of units. The differential species group, it was classified into total of 7 species group units including Thuja koraiensis and Smilacina bicolor differential species group. The mean importance percentage (MIP) in VU 1 was shown Pinus koraiensis (24.1), A. nephrolepis (23.6) and Betula ermanii (10.5), and in VU 2, it was P. koraiensis (26.2), A. nephrolepis (20.8) and B. ermanii (10.5), and in VU 3, it was P. koraiensis (22.2), A. nephrolepis (16.4), B. ermanii (13.5) in the order, respectively. As for importance values of P. koraiensis and A. nephrolepis representative competition species, were different at each layers. The former (46.7) was higher than the latter (26.1) in tree layer, but the latter was higher than the former in subtree and, shrub and herb layers of VU 1. VU 2 and VU 3 also indicated the same tendency. P. pumila group and A. decursiva group were shown to have positive correlation with altitude and present species, respectively. The designated as the endemic plant was 11 taxa and, as the rare plant 24 taxa and 62.5% of vascular plant was northern plant species. It is necessary to continue further long-term monitoring of 3 vegetation units, IV of main competition species (P. koraiensis and A. nephrolepis) with stratum and main specific species (rare plants and endemic plants) considering vegetation science and, population and community ecology approaches.

• Korean Journal of Plant Taxonomy
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• v.45 no.1
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• pp.96-107
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• 2015

This study presents a comprehensive account of the flora of hydrophytes and hygrophytes on Jeju Island. This approach aims to contribute to fundamental information about the conservation of plant diversity in wetlands. Field surveys were carried out from May of 2010 to September of 2014 in various types of wetlands on Jeju Island. A total of 189 taxa, including 52 families, 98 genera, 181 species, 6 varieties, 2 forma and 189 taxa, were found. Among them, 99 taxa (38 families, 60 genera, 96 species and 3 varieties) were hydrophytes and 90 taxa (23 families, 45 genera, 85 species, 3 varieties and 2 forma) were hygrophytes. In addition, Eleocharis${\times}$yezoensis H. Hara (Cyperaceae) was newly added to the flora of Korea. The protected wild plants designated by the Ministry of the Environment were 5 taxa, including Brasenia schreberi J. F. Gmel. and Dysophylla yatabeana Makino. The endangered species found included one Critically Endangered Species (i.e., Mankyua chejuense B.-Y. Sun, M. H. Kim & C. H. Kim), 5 Endangered Species (e.g., Lysimachia leucantha Miq.) and 7 Vulnerable Species (e.g., Tillaea aquatica L.). The floristic regional indicator plants found in this area were 44 taxa comprising 10 taxa of grade V, 7 taxa grade IV, 15 taxa of grade III, 5 taxa of grade II, and 7 taxa of grade I. The identified naturalized plants were 10 taxa; 8 families, 8 genera, 9 species and 1 variety. In the wetlands on Jeju Island, high plant species diversity was shown, and a number of rare plants and phytogeographically important plants were found. For these reasons, we consider that the wetlands on Jeju Island are very important areas from a botanical point of view. It is necessary to establish specific protection and maintenance policies in an effort to conserve these wetlands and species.

• Asian Journal of Turfgrass Science
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• v.9 no.4
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• pp.293-316
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• 1995

Nitrogen fertilization and cutting practice were studied on turfgrasses and cover plants to investigate the possibility of maintaining green color during the growing season. Research also involved the effect of the nitrogen on a few morphological characteristics of leaf performance elements which might give an information to coloration and life-span of turf leaves. Treatments in the first experiment undertaken on pot included one N level: 350kgN /ha applied as compound fertilizer in split applications of one-half in mid-May and the rest both in late June and August, and four spring-summer cuts: late May, late June, late July and late August. The soil filled in pot a moderately well-drained sandy loam. In the second experiment(field observation) leaf length and width, inflorescence and flowering, and color performance were also investigated. With nitrogen fertilizer applied on turfs, desirable turf color was maintained during a period of poor coloration in specific seasons such as mid-summer for cool season grasses and late fall for warm season grasses comparing to the non-treatment. However, this was not stimulated by cutting treatment to nitrogen status existed. Cutting effect on coloration was more remarkable in both Korean lawngrass and Manilagrass than in cool season turfgrasses such as Italian rye-grass, perennial ryegrass and tall fescue. Especially down-slide of leaf color in cool season turfgrasses could he detected in mid-summer /early fall season ranging up to mid-September. In early November as well as mid-September, Italian ryegrass, perennial ryegrass and tall fes-cue retained a high level of green color as followed by nitrogen application and cutting treatment, and little detectable variation of leaf color notation between cool season turfgrasses was obtained. However, Korean la'vngrass and Manilagrass failed to retain the green color until early November. Color notations in cool season turfgrasses investigated early November on the final date of the experiment ranged from 5 GY 3/1 to 4/8 in 'Ramultra' Italian ryegrass, 'Reveile' perennial ryegrass and 'Arid' tall fescue, but those in Zoysiagrasses were 7.5 YR 4/8 in Korean lawngrass and 2.5 y 5 /6 in Manilagrass. Life-span of leaves was shorter in Italian ryegrass, perennial ryegrass and tall fescue than in beth Korean lawngrass and Manilagrass with and without nitrogen application. In general, leaves appeared in early May had a long life-span than those appeared in late April or mid-June. Nitrogen application significantly prolonged the green color retaining period in perennial ryegrass, Italian ryegrass, Korean lawngrass and Manilagrass, and this was contrasted with the fact that there was no prolonged life-span of leaves emerging in early May and mid-June in tall fescue. SPAD reading values in 48 turfs and cover plants investigated in the field trial were increasing until late June and again decreasing till September. Increasing trends of reading value could be observed in the middle of October in most of grasses. On the other hand, clovers and reed canarygrasses did not restore their color values even in October. Color differences between inter-varieties, and inter-species occurred during the growing season under the field condition implicated that selection of species and /or cultivars for mixture should be taken into consideration. In Munsell color notation investigated in the final date in the middle of November, 32 cultivars belonged under the category of 5 GY and 10 cultivars under the category of 7.5 GY. This was implying that most of cool season turfs and cover plants grown in the center zone of Korean Peninsula which are able to utilize for landscape use can bear their reasonable green color by early or mid-November when properly managed. The applicable possibilities of SPAD readings and Munsell color notation to determine the color status of turfgrasses and cover plants used in this study were discussed.

• Journal of Intelligence and Information Systems
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• v.17 no.4
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• pp.213-226
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• 2011

In global supply chain, the scheduling problems of large products such as ships, airplanes, space shuttles, assembled constructions, and/or automobiles are complicated by nature. New scheduling systems are often developed in order to reduce inherent computational complexity. As a result, a problem can be decomposed into small sub-problems, problems that contain independently small scheduling systems integrating into the initial problem. As one of the authors experienced, DAS (Daewoo Shipbuilding Scheduling System) has adopted a two-layered hierarchical architecture. In the hierarchical architecture, individual scheduling systems composed of a high-level dock scheduler, DAS-ERECT and low-level assembly plant schedulers, DAS-PBS, DAS-3DS, DAS-NPS, and DAS-A7 try to search the best schedules under their own constraints. Moreover, the steep growth of communication technology and logistics enables it to introduce distributed multi-nation production plants by which different parts are produced by designated plants. Therefore vertical and lateral coordination among decomposed scheduling systems is necessary. No standard coordination mechanism of multiple scheduling systems exists, even though there are various scheduling systems existing in the area of scheduling research. Previous research regarding the coordination mechanism has mainly focused on external conversation without capacity model. Prior research has heavily focuses on agent-based coordination in the area of agent research. Yet, no scheduling domain has been developed. Previous research regarding the agent-based scheduling has paid its ample attention to internal coordination of scheduling process, a process that has not been efficient. In this study, we suggest a general framework for agent-based coordination of multiple scheduling systems in global supply chain. The purpose of this study was to design a standard coordination mechanism. To do so, we first define an individual scheduling agent responsible for their own plants and a meta-level coordination agent involved with each individual scheduling agent. We then suggest variables and values describing the individual scheduling agent and meta-level coordination agent. These variables and values are represented by Backus-Naur Form. Second, we suggest scheduling agent communication protocols for each scheduling agent topology classified into the system architectures, existence or nonexistence of coordinator, and directions of coordination. If there was a coordinating agent, an individual scheduling agent could communicate with another individual agent indirectly through the coordinator. On the other hand, if there was not any coordinating agent existing, an individual scheduling agent should communicate with another individual agent directly. To apply agent communication language specifically to the scheduling coordination domain, we had to additionally define an inner language, a language that suitably expresses scheduling coordination. A scheduling agent communication language is devised for the communication among agents independent of domain. We adopt three message layers which are ACL layer, scheduling coordination layer, and industry-specific layer. The ACL layer is a domain independent outer language layer. The scheduling coordination layer has terms necessary for scheduling coordination. The industry-specific layer expresses the industry specification. Third, in order to improve the efficiency of communication among scheduling agents and avoid possible infinite loops, we suggest a look-ahead load balancing model which supports to monitor participating agents and to analyze the status of the agents. To build the look-ahead load balancing model, the status of participating agents should be monitored. Most of all, the amount of sharing information should be considered. If complete information is collected, updating and maintenance cost of sharing information will be increasing although the frequency of communication will be decreasing. Therefore the level of detail and updating period of sharing information should be decided contingently. By means of this standard coordination mechanism, we can easily model coordination processes of multiple scheduling systems into supply chain. Finally, we apply this mechanism to shipbuilding domain and develop a prototype system which consists of a dock-scheduling agent, four assembly- plant-scheduling agents, and a meta-level coordination agent. A series of experiments using the real world data are used to empirically examine this mechanism. The results of this study show that the effect of agent-based platform on coordinated scheduling is evident in terms of the number of tardy jobs, tardiness, and makespan.

• Journal of Plant Biotechnology
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• v.30 no.1
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• pp.13-18
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• 2003

This study was carried out to investigate the expression patterns of foreign gene that controlled by tuber-specific patatin promoter in transgenic potatoes. Potato leaf disc cultured in vitro were transformed by the Agrobacterium strain LBA4404 containing pBl121 or pATGUS from potato cv. Desiree. In order to select the transgenic lines, gene-specific primers deduced from the NPTII were synthesized and used for polymerase chain reaction. The down part of the putative transgenic potatoes was transplanted weekly onto sucrose-enriched medium to accelerate the microtuber formation. RNA gel blot analysis was performed on the total RNAs obtained from tuber that had been harvested at a week interval. Also, histochemical assay was observed in the explants transformed with either pBI121 or pATGUS. Results showed that the transgenic plant containing pATGUS expressed GUS transcripts mainly at the tuber, not in stem, with the highest expression level in 5 weeks-grown microtubers. In contrast to pATGUS plants, the transformed plants with pBI121 showed an equal expression pattern throughout the whole developing stages. Consistent with RNA gel blot analysis, histochemical GUS staining and enzyme activity exhibited pATGUS transcripts were at the highest level in 5 weeks cultures. From these results, we suggest that the best stage to analyze the foreign gene introduced by patatin promoter into potato plants is at 5 weeks cultures after tuber formation.

• Research in Plant Disease
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• v.14 no.1
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• pp.15-20
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• 2008

Three isolates of Cucumber mosaic virus (CMV) were isolated from weed hosts showing typical mosaic symptoms, and some properties of the viruses were investigated. CMV isolates, designated as Is-CMV, Jd-CMV and Pla-CMV from Isodon inflexus, Jeffersonia dubia and Phryma leptostachya var. asiatica, respectively, were identified and characterized by biological reaction in several host plants, serological property, dsRNA analysis, reverse transcription-polymerase chain reaction (RT-PCR), restriction fragment-length polymorphism (RFLP). All isolates systemically infected in Nicotiana benthamiana, Cucurbita pepo cv. Black beauty and Cucumis sativus, and did not reveal any differences in these host plants between the isolates. However, remarkable difference in the symptoms was found between the CMVs in Capsicum annuum. Is-CMV induced an asymptomatic symptoms, while Jd-CMV and Pla-CMV produced severe mosaic symptoms in C. annuum plants. In dsRNA analysis, all isolates revealed four major bands with estimated molecular size of 3.4, 3.2, 2.1 and 1.0 kbp. The cDNAs of coat protein gene of the isolates were amplified by RT-PCR using a genus-specific single pair primers that designed to amplify a DNA fragment of approximately ranging from 938 to 966 bp. By restriction mapping analysis using RFLP of the RT-PCR products as well as by serological properties of gel diffusion test, the CMV isolates belong to a typical members of CMV subgroup IA. This is the first report on the occurrence of CMV in the three weed hosts.

• Journal of Bio-Environment Control
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• v.32 no.1
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• pp.23-33
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• 2023

Sucrose (suc) is a disaccharide that consists of glucose (glu) and fructose (fru). It is a carbohydrate source that acts as a nutrient molecule and a molecular signal that regulates gene expression and alters metabolites. This study aimed to evaluate whether suc-specific signaling induces an increase in bioactive compounds by exogenous suc absorption via roots or whether other factors, such as osmotic stress or biotic stress, are involved. To compare the osmotic stress induced by suc treatment, 4-week-old cultured mugwort plants were subjected to Hoagland nutrient solution with 10 mM, 30 mM, and 50 mM of suc or mannitol (man) for 3 days. Shoot fresh weight in suc and man treatments was not significantly different from the control. Both man and suc treatments increased the content of bioactive compounds in mugwort, but they displayed different enhancement patterns compared to the suc treatments. Mugwort extract treated with suc 50 mM effectively protected HepG2 liver cells damaged by ethanol and t-BHP. To compare the biotic stress induced by suc treatment, 3-week-old mugwort plants were subjected to microorganism and/or suc 30 mM with Hoagland nutrient solution. Microorganisms and/or suc 30 mM treatments showed no difference about the shoot fresh weight. However, sugar content in mugwort treated with suc 30 mM and microorganism with suc 30 mM treatment was significantly higher than that of the control. Suc 30 mM and microorganism with suc 30 mM were effective in enhancing bioactive compounds than microorganism treatment. These results suggest that mugwort plants can absorb exogenous suc via roots and the enhancement of bioactive compounds by suc treatment may result not from osmotic stress or biotic stress because of microorganism, but by suc-specific signaling.

• Journal of Bio-Environment Control
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• v.4 no.2
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• pp.136-143
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• 1995

Using a PEG- dextran two phase partition method, plasma and intracellular membrane separated from microsomal membrane of canola (Brassica napus) leaves have been fractionated by centrifugation. $K^{+}$- ATPase specific activity in the plasma membrane (U$_2$ phase) of plants grown at $25^{\circ}C$ and 1$0^{\circ}C$ were 6.6 and 4.6 times, respectively that of the microsomal membrane. Plasma membrane had a lower cytochrome- c- oxidase specific activity than the microsomal membrane or intracellular membrane, while intracellular membrane (L$_2$ phase) had a high cytochrome-c- oxidase but little $K^{+}$- ATPase specific activity. The plasma membrane of canola grown at 1$0^{\circ}C$ had higher 18:3 to 18:2 (linolenic to linoleic acid) ratio (29.2% ) and higher degree of unsaturation than that grown at $25^{\circ}C$ The double bond index of plasma membrane from canola grown at 1$0^{\circ}C$ increased by 8.9% relative to canola grown at $25^{\circ}C$. Similar, intracellular membrane increased by 19.7% at 1$0^{\circ}C$. Canola grown at 1$0^{\circ}C$ was lower in chlorophyll contents (17.3%) than that grown at $25^{\circ}C$. These changes in fatty acid unsaturation were attributable largely to change in Cl8 fatty acid, with major changes occurring in linolenic acid (18 :3) which might have a physiological role of membrane to adaptation on low temperature.ure.